Acinetobacter brisouii CIP 110357

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Moraxellaceae; Acinetobacter; Acinetobacter brisouii

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2927 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|V2UKC3|V2UKC3_9GAMM Thioredoxin domain-containing protein OS=Acinetobacter brisouii CIP 110357 OX=1341683 GN=P255_02785 PE=4 SV=1
YYY2 pKa = 10.09IDDD5 pKa = 3.76IQFSDDD11 pKa = 3.65TTLDDD16 pKa = 3.0EEE18 pKa = 4.35VKKK21 pKa = 10.81LVLLGTEEE29 pKa = 4.07EE30 pKa = 4.81DDD32 pKa = 3.42LRR34 pKa = 11.84AYYY37 pKa = 7.05TGSTISGEEE46 pKa = 4.02GDDD49 pKa = 5.61DD50 pKa = 3.89IHHH53 pKa = 6.68NIGSDDD59 pKa = 3.37IYYY62 pKa = 10.67GDDD65 pKa = 4.18QDDD68 pKa = 4.14LYYY71 pKa = 10.37DDD73 pKa = 4.59GNDDD77 pKa = 3.6HH78 pKa = 7.14EEE80 pKa = 4.43GADDD84 pKa = 3.58DDD86 pKa = 3.53LYYY89 pKa = 10.74GNGNDDD95 pKa = 3.52LIGGSGNDDD104 pKa = 4.32LDDD107 pKa = 4.64GDDD110 pKa = 5.53SDDD113 pKa = 3.46LYYY116 pKa = 10.83GTGDDD121 pKa = 4.79DD122 pKa = 4.15LNGGAGNDDD131 pKa = 4.01LDDD134 pKa = 4.96GDDD137 pKa = 5.43SDDD140 pKa = 3.82LNGGIGDDD148 pKa = 5.04DD149 pKa = 3.64LVGGTGDDD157 pKa = 3.68DD158 pKa = 3.77LIGGQGNDDD167 pKa = 3.85YYY169 pKa = 11.18FDDD172 pKa = 5.74GWGQDDD178 pKa = 3.58IQYYY182 pKa = 8.96EE183 pKa = 4.8KKK185 pKa = 10.38QTDDD189 pKa = 3.02DDD191 pKa = 4.07IEEE194 pKa = 5.16ININPDDD201 pKa = 3.33DD202 pKa = 5.11IVRR205 pKa = 11.84KKK207 pKa = 9.9GQDDD211 pKa = 3.06VVMHHH216 pKa = 7.58ITGDDD221 pKa = 3.27LIVEEE226 pKa = 4.82QFSDDD231 pKa = 3.88YY232 pKa = 11.34SNKKK236 pKa = 9.06INKKK240 pKa = 8.54RR241 pKa = 11.84FDDD244 pKa = 4.92DD245 pKa = 3.66TEEE248 pKa = 3.93DDD250 pKa = 3.74EE251 pKa = 5.06DD252 pKa = 3.87LNVQAVKKK260 pKa = 9.33TEEE263 pKa = 3.82DDD265 pKa = 4.5DD266 pKa = 4.96IEEE269 pKa = 4.5TTDDD273 pKa = 3.02HH274 pKa = 7.24DD275 pKa = 4.87IHHH278 pKa = 7.08GAGDDD283 pKa = 4.2DD284 pKa = 3.89IYYY287 pKa = 9.92RR288 pKa = 11.84PVYYY292 pKa = 10.85EEE294 pKa = 4.02PEEE297 pKa = 3.9QYYY300 pKa = 11.19YY301 pKa = 9.74YYY303 pKa = 10.74GEEE306 pKa = 4.45NDDD309 pKa = 3.57KK310 pKa = 10.43YYY312 pKa = 10.21TGYYY316 pKa = 10.28DDD318 pKa = 4.73GVGDDD323 pKa = 5.42DD324 pKa = 4.12EE325 pKa = 5.5EEE327 pKa = 4.54SGHHH331 pKa = 6.78LGGEEE336 pKa = 4.32NDDD339 pKa = 3.23LSGQGVLEEE348 pKa = 4.41QSGNDDD354 pKa = 2.9LTGQGKKK361 pKa = 10.37YYY363 pKa = 10.96GDDD366 pKa = 3.66QDDD369 pKa = 3.22LTLTGLANDDD379 pKa = 3.98EE380 pKa = 4.76GLLSGGAGDDD390 pKa = 5.21DD391 pKa = 3.66LTIDDD396 pKa = 3.98NQMDDD401 pKa = 4.72VNEEE405 pKa = 3.84YY406 pKa = 10.54QDDD409 pKa = 2.96FYYY412 pKa = 11.08DDD414 pKa = 2.86DDD416 pKa = 4.09YYY418 pKa = 11.4HH419 pKa = 7.31IQDDD423 pKa = 3.41EEE425 pKa = 4.06TEEE428 pKa = 4.06QRR430 pKa = 11.84AVYYY434 pKa = 10.64EEE436 pKa = 4.62GQGNDDD442 pKa = 3.31IYYY445 pKa = 11.02SFGDDD450 pKa = 4.01EE451 pKa = 3.66YYY453 pKa = 10.96FNLGDDD459 pKa = 3.93KKK461 pKa = 9.88DD462 pKa = 3.82IIEEE466 pKa = 4.08PAGQNYYY473 pKa = 10.37NVAVSFDDD481 pKa = 3.55LRR483 pKa = 11.84FGQGITTIDDD493 pKa = 3.38SLHHH497 pKa = 6.34YYY499 pKa = 7.24TDDD502 pKa = 3.25VIKKK506 pKa = 8.73HH507 pKa = 6.03NQTDDD512 pKa = 3.26ITIQNYYY519 pKa = 7.09TDDD522 pKa = 3.41HHH524 pKa = 6.35HH525 pKa = 6.55KK526 pKa = 10.54NEEE529 pKa = 3.91QFADDD534 pKa = 3.49TIWNNAYYY542 pKa = 9.63EEE544 pKa = 4.06HHH546 pKa = 5.01TYYY549 pKa = 10.53HH550 pKa = 5.82TANNDDD556 pKa = 3.53EE557 pKa = 4.13WGYYY561 pKa = 11.35NTDDD565 pKa = 3.05EE566 pKa = 4.47FEEE569 pKa = 4.23DDD571 pKa = 3.7GDDD574 pKa = 3.85DD575 pKa = 3.7KK576 pKa = 11.71YYY578 pKa = 11.0GAGNDDD584 pKa = 3.83IYYY587 pKa = 9.75QAGEEE592 pKa = 4.75DD593 pKa = 3.8LWGQAGNDDD602 pKa = 3.63LYYY605 pKa = 11.24GIGADDD611 pKa = 3.18YY612 pKa = 11.34EEE614 pKa = 5.01NEEE617 pKa = 4.94DDD619 pKa = 4.56DD620 pKa = 4.85LYYY623 pKa = 10.73DDD625 pKa = 4.04DDD627 pKa = 3.41DDD629 pKa = 3.2LYYY632 pKa = 10.55GAGNDDD638 pKa = 3.2LYYY641 pKa = 10.42GQGDDD646 pKa = 4.52DD647 pKa = 3.12LRR649 pKa = 11.84GGTCHHH655 pKa = 6.9DD656 pKa = 4.36LDDD659 pKa = 4.12GAGNDDD665 pKa = 3.12YYY667 pKa = 11.47YYY669 pKa = 11.2YY670 pKa = 10.68ADDD673 pKa = 3.59TEEE676 pKa = 4.76IDDD679 pKa = 3.38TGGGTDDD686 pKa = 3.74LWLMDDD692 pKa = 3.49GITEEE697 pKa = 4.39DD698 pKa = 3.78IKKK701 pKa = 10.0TKKK704 pKa = 10.13EE705 pKa = 3.39NDDD708 pKa = 4.24IVTIDDD714 pKa = 3.6NPNQSVRR721 pKa = 11.84VKKK724 pKa = 10.81DD725 pKa = 3.55HH726 pKa = 6.43LGGEEE731 pKa = 3.97KK732 pKa = 10.07ISSVQPNGGYYY743 pKa = 9.12ITAAQIAVKKK753 pKa = 10.46NASAGGLSDDD763 pKa = 4.31EEE765 pKa = 5.64DDD767 pKa = 3.75IYYY770 pKa = 10.78YYY772 pKa = 8.18TGAMTITEEE781 pKa = 3.96SGNDDD786 pKa = 3.02VLFKKK791 pKa = 11.37GITFSQVGNYYY802 pKa = 7.2TKKK805 pKa = 10.89GDDD808 pKa = 3.74DD809 pKa = 4.56ILKKK813 pKa = 10.0NGSNTNKKK821 pKa = 8.44TIKKK825 pKa = 10.89FFLAGNYYY833 pKa = 9.38VEEE836 pKa = 4.23LQFEEE841 pKa = 4.55GGQLTAEEE849 pKa = 4.37IFGAFGLTIPSGTTPIPPNPVGDDD873 pKa = 3.69TYYY876 pKa = 10.57YYY878 pKa = 11.79SGEEE882 pKa = 3.9TITEEE887 pKa = 3.75SGNDDD892 pKa = 3.11KK893 pKa = 11.23IFKKK897 pKa = 10.95GITFNQVGNYYY908 pKa = 6.74TKKK911 pKa = 10.89GDDD914 pKa = 3.74DD915 pKa = 4.56ILKKK919 pKa = 10.08NGSDDD924 pKa = 3.22NTVIVKKK931 pKa = 10.0FFLAGQYYY939 pKa = 10.14VEEE942 pKa = 4.28FEEE945 pKa = 4.38EEE947 pKa = 3.96GGKKK951 pKa = 7.63TAEEE955 pKa = 4.06IFGAFGITMPQQSAPANTSPEEE977 pKa = 3.99MDDD980 pKa = 4.28DDD982 pKa = 4.27SNTIYYY988 pKa = 10.66YYY990 pKa = 10.66SGSMIIDDD998 pKa = 3.39EE999 pKa = 4.69LGTDDD1004 pKa = 3.13VVFGNGISFSQVGNYYY1020 pKa = 7.45TQSGDDD1026 pKa = 3.73DD1027 pKa = 4.87ILKKK1031 pKa = 10.05NGSNSNKKK1039 pKa = 8.24TVKKK1043 pKa = 10.9DD1044 pKa = 4.06FLGGAHHH1051 pKa = 5.56EE1052 pKa = 4.3EEE1054 pKa = 4.48FNFEEE1059 pKa = 4.06GGSISSQQIYYY1070 pKa = 8.4VFGVDDD1076 pKa = 3.37HH1077 pKa = 6.6TNAEEE1082 pKa = 4.12DD1083 pKa = 3.71EE1084 pKa = 4.58TSIVVGDDD1092 pKa = 3.89DDD1094 pKa = 6.12DD1095 pKa = 5.24ILSSNAAVSEEE1106 pKa = 4.02FVLNEEE1112 pKa = 4.04NDDD1115 pKa = 3.51LEEE1118 pKa = 4.55LLNASGEEE1126 pKa = 4.36AVDDD1130 pKa = 3.62YY1131 pKa = 10.4TDDD1134 pKa = 4.16DDD1136 pKa = 4.03TEEE1139 pKa = 4.18DD1140 pKa = 4.04IDDD1143 pKa = 4.06SQILDDD1149 pKa = 3.74QATSSNLSDDD1159 pKa = 4.25YY1160 pKa = 11.36EEE1162 pKa = 3.82TYYY1165 pKa = 9.87ANAKKK1170 pKa = 9.29NTLSLRR1176 pKa = 11.84DDD1178 pKa = 3.76LNVTVKKK1185 pKa = 10.84DD1186 pKa = 3.61LIFTNQAEEE1195 pKa = 4.36LSITDDD1201 pKa = 3.68MMNQAIIY

Molecular weight:
129.95 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|V2UQ88|V2UQ88_9GAMM Uncharacterized protein OS=Acinetobacter brisouii CIP 110357 OX=1341683 GN=P255_01293 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.55RR3 pKa = 11.84TFQPSEE9 pKa = 3.97LKK11 pKa = 10.13RR12 pKa = 11.84KK13 pKa = 8.98RR14 pKa = 11.84VHH16 pKa = 6.36GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4AGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.18GRR39 pKa = 11.84HH40 pKa = 5.1SLTVV44 pKa = 3.06

Molecular weight:
5.18 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2927

0

2927

896350

30

1625

306.2

34.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.925 ± 0.048

1.011 ± 0.015

5.033 ± 0.039

5.626 ± 0.043

4.17 ± 0.034

6.487 ± 0.051

2.618 ± 0.025

6.688 ± 0.038

5.159 ± 0.038

10.692 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.513 ± 0.023

4.157 ± 0.035

3.981 ± 0.027

6.056 ± 0.068

4.445 ± 0.035

5.974 ± 0.038

5.253 ± 0.031

6.746 ± 0.039

1.285 ± 0.02

3.181 ± 0.025

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski