Acinetobacter brisouii CIP 110357
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2927 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|V2UKC3|V2UKC3_9GAMM Thioredoxin domain-containing protein OS=Acinetobacter brisouii CIP 110357 OX=1341683 GN=P255_02785 PE=4 SV=1
YYY2 pKa = 10.09 IDDD5 pKa = 3.76 IQFSDDD11 pKa = 3.65 TTLDDD16 pKa = 3.0 EEE18 pKa = 4.35 VKKK21 pKa = 10.81 LVLLGTEEE29 pKa = 4.07 EE30 pKa = 4.81 DDD32 pKa = 3.42 LRR34 pKa = 11.84 AYYY37 pKa = 7.05 TGSTISGEEE46 pKa = 4.02 GDDD49 pKa = 5.61 DD50 pKa = 3.89 IHHH53 pKa = 6.68 NIGSDDD59 pKa = 3.37 IYYY62 pKa = 10.67 GDDD65 pKa = 4.18 QDDD68 pKa = 4.14 LYYY71 pKa = 10.37 DDD73 pKa = 4.59 GNDDD77 pKa = 3.6 HH78 pKa = 7.14 EEE80 pKa = 4.43 GADDD84 pKa = 3.58 DDD86 pKa = 3.53 LYYY89 pKa = 10.74 GNGNDDD95 pKa = 3.52 LIGGSGNDDD104 pKa = 4.32 LDDD107 pKa = 4.64 GDDD110 pKa = 5.53 SDDD113 pKa = 3.46 LYYY116 pKa = 10.83 GTGDDD121 pKa = 4.79 DD122 pKa = 4.15 LNGGAGNDDD131 pKa = 4.01 LDDD134 pKa = 4.96 GDDD137 pKa = 5.43 SDDD140 pKa = 3.82 LNGGIGDDD148 pKa = 5.04 DD149 pKa = 3.64 LVGGTGDDD157 pKa = 3.68 DD158 pKa = 3.77 LIGGQGNDDD167 pKa = 3.85 YYY169 pKa = 11.18 FDDD172 pKa = 5.74 GWGQDDD178 pKa = 3.58 IQYYY182 pKa = 8.96 EE183 pKa = 4.8 KKK185 pKa = 10.38 QTDDD189 pKa = 3.02 DDD191 pKa = 4.07 IEEE194 pKa = 5.16 ININPDDD201 pKa = 3.33 DD202 pKa = 5.11 IVRR205 pKa = 11.84 KKK207 pKa = 9.9 GQDDD211 pKa = 3.06 VVMHHH216 pKa = 7.58 ITGDDD221 pKa = 3.27 LIVEEE226 pKa = 4.82 QFSDDD231 pKa = 3.88 YY232 pKa = 11.34 SNKKK236 pKa = 9.06 INKKK240 pKa = 8.54 RR241 pKa = 11.84 FDDD244 pKa = 4.92 DD245 pKa = 3.66 TEEE248 pKa = 3.93 DDD250 pKa = 3.74 EE251 pKa = 5.06 DD252 pKa = 3.87 LNVQAVKKK260 pKa = 9.33 TEEE263 pKa = 3.82 DDD265 pKa = 4.5 DD266 pKa = 4.96 IEEE269 pKa = 4.5 TTDDD273 pKa = 3.02 HH274 pKa = 7.24 DD275 pKa = 4.87 IHHH278 pKa = 7.08 GAGDDD283 pKa = 4.2 DD284 pKa = 3.89 IYYY287 pKa = 9.92 RR288 pKa = 11.84 PVYYY292 pKa = 10.85 EEE294 pKa = 4.02 PEEE297 pKa = 3.9 QYYY300 pKa = 11.19 YY301 pKa = 9.74 YYY303 pKa = 10.74 GEEE306 pKa = 4.45 NDDD309 pKa = 3.57 KK310 pKa = 10.43 YYY312 pKa = 10.21 TGYYY316 pKa = 10.28 DDD318 pKa = 4.73 GVGDDD323 pKa = 5.42 DD324 pKa = 4.12 EE325 pKa = 5.5 EEE327 pKa = 4.54 SGHHH331 pKa = 6.78 LGGEEE336 pKa = 4.32 NDDD339 pKa = 3.23 LSGQGVLEEE348 pKa = 4.41 QSGNDDD354 pKa = 2.9 LTGQGKKK361 pKa = 10.37 YYY363 pKa = 10.96 GDDD366 pKa = 3.66 QDDD369 pKa = 3.22 LTLTGLANDDD379 pKa = 3.98 EE380 pKa = 4.76 GLLSGGAGDDD390 pKa = 5.21 DD391 pKa = 3.66 LTIDDD396 pKa = 3.98 NQMDDD401 pKa = 4.72 VNEEE405 pKa = 3.84 YY406 pKa = 10.54 QDDD409 pKa = 2.96 FYYY412 pKa = 11.08 DDD414 pKa = 2.86 DDD416 pKa = 4.09 YYY418 pKa = 11.4 HH419 pKa = 7.31 IQDDD423 pKa = 3.41 EEE425 pKa = 4.06 TEEE428 pKa = 4.06 QRR430 pKa = 11.84 AVYYY434 pKa = 10.64 EEE436 pKa = 4.62 GQGNDDD442 pKa = 3.31 IYYY445 pKa = 11.02 SFGDDD450 pKa = 4.01 EE451 pKa = 3.66 YYY453 pKa = 10.96 FNLGDDD459 pKa = 3.93 KKK461 pKa = 9.88 DD462 pKa = 3.82 IIEEE466 pKa = 4.08 PAGQNYYY473 pKa = 10.37 NVAVSFDDD481 pKa = 3.55 LRR483 pKa = 11.84 FGQGITTIDDD493 pKa = 3.38 SLHHH497 pKa = 6.34 YYY499 pKa = 7.24 TDDD502 pKa = 3.25 VIKKK506 pKa = 8.73 HH507 pKa = 6.03 NQTDDD512 pKa = 3.26 ITIQNYYY519 pKa = 7.09 TDDD522 pKa = 3.41 HHH524 pKa = 6.35 HH525 pKa = 6.55 KK526 pKa = 10.54 NEEE529 pKa = 3.91 QFADDD534 pKa = 3.49 TIWNNAYYY542 pKa = 9.63 EEE544 pKa = 4.06 HHH546 pKa = 5.01 TYYY549 pKa = 10.53 HH550 pKa = 5.82 TANNDDD556 pKa = 3.53 EE557 pKa = 4.13 WGYYY561 pKa = 11.35 NTDDD565 pKa = 3.05 EE566 pKa = 4.47 FEEE569 pKa = 4.23 DDD571 pKa = 3.7 GDDD574 pKa = 3.85 DD575 pKa = 3.7 KK576 pKa = 11.71 YYY578 pKa = 11.0 GAGNDDD584 pKa = 3.83 IYYY587 pKa = 9.75 QAGEEE592 pKa = 4.75 DD593 pKa = 3.8 LWGQAGNDDD602 pKa = 3.63 LYYY605 pKa = 11.24 GIGADDD611 pKa = 3.18 YY612 pKa = 11.34 EEE614 pKa = 5.01 NEEE617 pKa = 4.94 DDD619 pKa = 4.56 DD620 pKa = 4.85 LYYY623 pKa = 10.73 DDD625 pKa = 4.04 DDD627 pKa = 3.41 DDD629 pKa = 3.2 LYYY632 pKa = 10.55 GAGNDDD638 pKa = 3.2 LYYY641 pKa = 10.42 GQGDDD646 pKa = 4.52 DD647 pKa = 3.12 LRR649 pKa = 11.84 GGTCHHH655 pKa = 6.9 DD656 pKa = 4.36 LDDD659 pKa = 4.12 GAGNDDD665 pKa = 3.12 YYY667 pKa = 11.47 YYY669 pKa = 11.2 YY670 pKa = 10.68 ADDD673 pKa = 3.59 TEEE676 pKa = 4.76 IDDD679 pKa = 3.38 TGGGTDDD686 pKa = 3.74 LWLMDDD692 pKa = 3.49 GITEEE697 pKa = 4.39 DD698 pKa = 3.78 IKKK701 pKa = 10.0 TKKK704 pKa = 10.13 EE705 pKa = 3.39 NDDD708 pKa = 4.24 IVTIDDD714 pKa = 3.6 NPNQSVRR721 pKa = 11.84 VKKK724 pKa = 10.81 DD725 pKa = 3.55 HH726 pKa = 6.43 LGGEEE731 pKa = 3.97 KK732 pKa = 10.07 ISSVQPNGGYYY743 pKa = 9.12 ITAAQIAVKKK753 pKa = 10.46 NASAGGLSDDD763 pKa = 4.31 EEE765 pKa = 5.64 DDD767 pKa = 3.75 IYYY770 pKa = 10.78 YYY772 pKa = 8.18 TGAMTITEEE781 pKa = 3.96 SGNDDD786 pKa = 3.02 VLFKKK791 pKa = 11.37 GITFSQVGNYYY802 pKa = 7.2 TKKK805 pKa = 10.89 GDDD808 pKa = 3.74 DD809 pKa = 4.56 ILKKK813 pKa = 10.0 NGSNTNKKK821 pKa = 8.44 TIKKK825 pKa = 10.89 FFLAGNYYY833 pKa = 9.38 VEEE836 pKa = 4.23 LQFEEE841 pKa = 4.55 GGQLTAEEE849 pKa = 4.37 IFGAFGLTIPSGTTPIPPNPVGDDD873 pKa = 3.69 TYYY876 pKa = 10.57 YYY878 pKa = 11.79 SGEEE882 pKa = 3.9 TITEEE887 pKa = 3.75 SGNDDD892 pKa = 3.11 KK893 pKa = 11.23 IFKKK897 pKa = 10.95 GITFNQVGNYYY908 pKa = 6.74 TKKK911 pKa = 10.89 GDDD914 pKa = 3.74 DD915 pKa = 4.56 ILKKK919 pKa = 10.08 NGSDDD924 pKa = 3.22 NTVIVKKK931 pKa = 10.0 FFLAGQYYY939 pKa = 10.14 VEEE942 pKa = 4.28 FEEE945 pKa = 4.38 EEE947 pKa = 3.96 GGKKK951 pKa = 7.63 TAEEE955 pKa = 4.06 IFGAFGITMPQQSAPANTSPEEE977 pKa = 3.99 MDDD980 pKa = 4.28 DDD982 pKa = 4.27 SNTIYYY988 pKa = 10.66 YYY990 pKa = 10.66 SGSMIIDDD998 pKa = 3.39 EE999 pKa = 4.69 LGTDDD1004 pKa = 3.13 VVFGNGISFSQVGNYYY1020 pKa = 7.45 TQSGDDD1026 pKa = 3.73 DD1027 pKa = 4.87 ILKKK1031 pKa = 10.05 NGSNSNKKK1039 pKa = 8.24 TVKKK1043 pKa = 10.9 DD1044 pKa = 4.06 FLGGAHHH1051 pKa = 5.56 EE1052 pKa = 4.3 EEE1054 pKa = 4.48 FNFEEE1059 pKa = 4.06 GGSISSQQIYYY1070 pKa = 8.4 VFGVDDD1076 pKa = 3.37 HH1077 pKa = 6.6 TNAEEE1082 pKa = 4.12 DD1083 pKa = 3.71 EE1084 pKa = 4.58 TSIVVGDDD1092 pKa = 3.89 DDD1094 pKa = 6.12 DD1095 pKa = 5.24 ILSSNAAVSEEE1106 pKa = 4.02 FVLNEEE1112 pKa = 4.04 NDDD1115 pKa = 3.51 LEEE1118 pKa = 4.55 LLNASGEEE1126 pKa = 4.36 AVDDD1130 pKa = 3.62 YY1131 pKa = 10.4 TDDD1134 pKa = 4.16 DDD1136 pKa = 4.03 TEEE1139 pKa = 4.18 DD1140 pKa = 4.04 IDDD1143 pKa = 4.06 SQILDDD1149 pKa = 3.74 QATSSNLSDDD1159 pKa = 4.25 YY1160 pKa = 11.36 EEE1162 pKa = 3.82 TYYY1165 pKa = 9.87 ANAKKK1170 pKa = 9.29 NTLSLRR1176 pKa = 11.84 DDD1178 pKa = 3.76 LNVTVKKK1185 pKa = 10.84 DD1186 pKa = 3.61 LIFTNQAEEE1195 pKa = 4.36 LSITDDD1201 pKa = 3.68 MMNQAIIY
Molecular weight: 129.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.682
IPC2_protein 3.656
IPC_protein 3.719
Toseland 3.478
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.656
Rodwell 3.541
Grimsley 3.389
Solomon 3.719
Lehninger 3.681
Nozaki 3.821
DTASelect 4.101
Thurlkill 3.528
EMBOSS 3.668
Sillero 3.846
Patrickios 1.049
IPC_peptide 3.706
IPC2_peptide 3.821
IPC2.peptide.svr19 3.754
Protein with the highest isoelectric point:
>tr|V2UQ88|V2UQ88_9GAMM Uncharacterized protein OS=Acinetobacter brisouii CIP 110357 OX=1341683 GN=P255_01293 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.55 RR3 pKa = 11.84 TFQPSEE9 pKa = 3.97 LKK11 pKa = 10.13 RR12 pKa = 11.84 KK13 pKa = 8.98 RR14 pKa = 11.84 VHH16 pKa = 6.36 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 AGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.18 GRR39 pKa = 11.84 HH40 pKa = 5.1 SLTVV44 pKa = 3.06
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.402
IPC2_protein 10.862
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.31
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.047
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.077
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2927
0
2927
896350
30
1625
306.2
34.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.925 ± 0.048
1.011 ± 0.015
5.033 ± 0.039
5.626 ± 0.043
4.17 ± 0.034
6.487 ± 0.051
2.618 ± 0.025
6.688 ± 0.038
5.159 ± 0.038
10.692 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.513 ± 0.023
4.157 ± 0.035
3.981 ± 0.027
6.056 ± 0.068
4.445 ± 0.035
5.974 ± 0.038
5.253 ± 0.031
6.746 ± 0.039
1.285 ± 0.02
3.181 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here