Imjin virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Hantaviridae; Mammantavirinae; Thottimvirus; Imjin thottimvirus

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A075IK44|A0A075IK44_9VIRU Replicase OS=Imjin virus OX=467989 PE=4 SV=1
MM1 pKa = 6.87AQSRR5 pKa = 11.84ITRR8 pKa = 11.84EE9 pKa = 4.31DD10 pKa = 3.12IQKK13 pKa = 10.87LEE15 pKa = 4.13DD16 pKa = 3.25AKK18 pKa = 11.07KK19 pKa = 10.39QIEE22 pKa = 4.27IQLSSSIQKK31 pKa = 10.2LATEE35 pKa = 4.91TEE37 pKa = 4.38QFGPDD42 pKa = 3.8PDD44 pKa = 5.6PIQLQAVRR52 pKa = 11.84QRR54 pKa = 11.84SSVIEE59 pKa = 4.4GYY61 pKa = 9.73KK62 pKa = 10.44EE63 pKa = 3.74KK64 pKa = 10.92LRR66 pKa = 11.84DD67 pKa = 3.68LEE69 pKa = 4.43GQLSIAYY76 pKa = 7.64ATFADD81 pKa = 4.23EE82 pKa = 4.21EE83 pKa = 4.44EE84 pKa = 4.13EE85 pKa = 4.04RR86 pKa = 11.84KK87 pKa = 9.87KK88 pKa = 10.73KK89 pKa = 10.7RR90 pKa = 11.84EE91 pKa = 3.84VPGVEE96 pKa = 3.78EE97 pKa = 5.05GDD99 pKa = 3.83YY100 pKa = 11.11LSQKK104 pKa = 10.51SSLRR108 pKa = 11.84YY109 pKa = 10.4GNVIDD114 pKa = 5.82LNPLNLEE121 pKa = 4.1EE122 pKa = 5.2PAGQTANWNRR132 pKa = 11.84IFEE135 pKa = 4.6YY136 pKa = 10.11IATITQVLLLKK147 pKa = 10.48GLYY150 pKa = 9.91ILTTRR155 pKa = 11.84GRR157 pKa = 11.84QTSKK161 pKa = 10.99DD162 pKa = 3.61NKK164 pKa = 7.3GTRR167 pKa = 11.84MKK169 pKa = 10.89LKK171 pKa = 10.31DD172 pKa = 4.19DD173 pKa = 3.86SCMEE177 pKa = 4.57TINGIQRR184 pKa = 11.84HH185 pKa = 4.93KK186 pKa = 10.49FLYY189 pKa = 10.03ISLPTSQSSIQDD201 pKa = 3.63DD202 pKa = 4.15EE203 pKa = 4.38LTPGRR208 pKa = 11.84FRR210 pKa = 11.84TIVSGLLPNEE220 pKa = 4.18IKK222 pKa = 10.51SKK224 pKa = 11.03KK225 pKa = 10.11LMSPVMGVIGFLNLTEE241 pKa = 3.85VWMDD245 pKa = 3.68IIDD248 pKa = 5.36RR249 pKa = 11.84IMTDD253 pKa = 2.47QCDD256 pKa = 3.55YY257 pKa = 8.19MTQDD261 pKa = 3.57KK262 pKa = 11.07SNPTASTNRR271 pKa = 11.84AYY273 pKa = 10.54FKK275 pKa = 10.58EE276 pKa = 4.04RR277 pKa = 11.84QNTINSLNLPDD288 pKa = 5.17LKK290 pKa = 10.58QLRR293 pKa = 11.84TDD295 pKa = 3.93AEE297 pKa = 4.33RR298 pKa = 11.84KK299 pKa = 9.72HH300 pKa = 5.93YY301 pKa = 10.83LPDD304 pKa = 5.31DD305 pKa = 3.91IEE307 pKa = 4.63TEE309 pKa = 4.21CVPWIFANAPDD320 pKa = 4.11RR321 pKa = 11.84CPPTVLLVAGIPEE334 pKa = 4.66LGAFFALMQDD344 pKa = 2.92IRR346 pKa = 11.84SGILASNLRR355 pKa = 11.84GTAEE359 pKa = 3.79EE360 pKa = 4.37KK361 pKa = 10.25IARR364 pKa = 11.84KK365 pKa = 9.81SSFYY369 pKa = 10.31QSYY372 pKa = 10.27IRR374 pKa = 11.84RR375 pKa = 11.84TQSMGLNCDD384 pKa = 2.99QKK386 pKa = 10.76IIHH389 pKa = 7.06IYY391 pKa = 9.25MDD393 pKa = 3.71YY394 pKa = 11.21LGTFCVDD401 pKa = 3.59HH402 pKa = 6.9FNLGDD407 pKa = 5.38DD408 pKa = 4.26MDD410 pKa = 5.66PDD412 pKa = 4.46LKK414 pKa = 10.77IKK416 pKa = 10.53AQALLDD422 pKa = 3.93KK423 pKa = 10.22KK424 pKa = 10.79VKK426 pKa = 10.2EE427 pKa = 4.29ISTQEE432 pKa = 4.13PIKK435 pKa = 10.86LL436 pKa = 3.59

Molecular weight:
49.72 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A075II01|A0A075II01_9VIRU Nucleocapsid protein OS=Imjin virus OX=467989 PE=3 SV=1
MM1 pKa = 7.46NNGFIIVLLQSLLQVYY17 pKa = 10.0CSTHH21 pKa = 5.54SRR23 pKa = 11.84ITLEE27 pKa = 4.17CPHH30 pKa = 7.08YY31 pKa = 10.28GVSDD35 pKa = 3.85AEE37 pKa = 4.46YY38 pKa = 9.21VWGKK42 pKa = 10.16VKK44 pKa = 10.43IGRR47 pKa = 11.84FTIDD51 pKa = 4.48DD52 pKa = 3.44IKK54 pKa = 10.93HH55 pKa = 5.21IQFEE59 pKa = 4.59NTCPFSITGHH69 pKa = 5.32NVQTGMKK76 pKa = 9.15IISWTEE82 pKa = 3.8KK83 pKa = 10.19EE84 pKa = 4.21NHH86 pKa = 6.97DD87 pKa = 3.8SSAVYY92 pKa = 9.41FDD94 pKa = 4.33KK95 pKa = 11.28VATHH99 pKa = 5.87SEE101 pKa = 3.88KK102 pKa = 10.49TGDD105 pKa = 3.76FKK107 pKa = 11.43SLCGSVSYY115 pKa = 8.86LTYY118 pKa = 9.87PFKK121 pKa = 11.05SGSSYY126 pKa = 11.08CLNLHH131 pKa = 6.86CNQTVCDD138 pKa = 3.79TKK140 pKa = 11.22VIVLIKK146 pKa = 10.19AISWVAYY153 pKa = 9.0DD154 pKa = 4.13SCVLYY159 pKa = 10.92HH160 pKa = 7.21KK161 pKa = 10.11DD162 pKa = 3.59IKK164 pKa = 10.89VQLDD168 pKa = 3.74FKK170 pKa = 10.23KK171 pKa = 7.54THH173 pKa = 5.8CVKK176 pKa = 10.73SVLHH180 pKa = 6.09EE181 pKa = 4.4KK182 pKa = 10.5KK183 pKa = 10.45CFQPDD188 pKa = 3.14FTTAFNPKK196 pKa = 9.36LHH198 pKa = 6.59MKK200 pKa = 10.39FSLPSVCLFMPSDD213 pKa = 3.9GNAEE217 pKa = 4.02GTKK220 pKa = 10.09EE221 pKa = 3.96IVGQDD226 pKa = 2.96SCTDD230 pKa = 2.86IKK232 pKa = 11.36LNGWYY237 pKa = 9.93SCIVGHH243 pKa = 6.02YY244 pKa = 11.07SMIFVHH250 pKa = 6.73SNGKK254 pKa = 8.06MRR256 pKa = 11.84QIHH259 pKa = 6.8LNTKK263 pKa = 8.53FRR265 pKa = 11.84KK266 pKa = 9.29NPQGDD271 pKa = 3.59DD272 pKa = 3.44HH273 pKa = 8.55SEE275 pKa = 3.87LRR277 pKa = 11.84SGSTHH282 pKa = 6.62IIGYY286 pKa = 10.49KK287 pKa = 10.68NMTTVSNKK295 pKa = 9.66VNTLAAFKK303 pKa = 10.73GATDD307 pKa = 3.9YY308 pKa = 11.8SSLFYY313 pKa = 10.85NIQNTDD319 pKa = 3.25KK320 pKa = 11.15KK321 pKa = 10.04PLLLAGYY328 pKa = 7.5TPTFHH333 pKa = 6.82WRR335 pKa = 11.84EE336 pKa = 4.38CGTHH340 pKa = 7.38LIPAVWTSHH349 pKa = 5.35IDD351 pKa = 3.1VWGTIIEE358 pKa = 4.13SDD360 pKa = 3.64EE361 pKa = 4.37CNIFCTLSGPGASCEE376 pKa = 4.11AFSPTGIFLLNSTTCLIPHH395 pKa = 5.49THH397 pKa = 6.41RR398 pKa = 11.84FKK400 pKa = 11.42GLGDD404 pKa = 3.85QITFICQRR412 pKa = 11.84VDD414 pKa = 2.86TDD416 pKa = 5.05LEE418 pKa = 5.04FYY420 pKa = 11.17CNGRR424 pKa = 11.84HH425 pKa = 6.35KK426 pKa = 10.52IIRR429 pKa = 11.84TKK431 pKa = 9.94TLIIGQCIQTFTSFFSLFPGVAHH454 pKa = 6.56SLAVEE459 pKa = 4.11LCVPGFHH466 pKa = 7.18GFITVCIVSTFCFGWLWIPGLTWLILQLLKK496 pKa = 10.89FIVLFVSTNSVDD508 pKa = 3.57QRR510 pKa = 11.84FKK512 pKa = 11.25QILQKK517 pKa = 10.56IKK519 pKa = 10.54AEE521 pKa = 3.92YY522 pKa = 10.05RR523 pKa = 11.84LTIGDD528 pKa = 4.32TSCEE532 pKa = 4.09FCKK535 pKa = 10.71HH536 pKa = 5.05EE537 pKa = 4.59CQTSLEE543 pKa = 4.26SDD545 pKa = 3.51SHH547 pKa = 8.4ILYY550 pKa = 10.09CKK552 pKa = 9.88QGKK555 pKa = 9.1CPYY558 pKa = 8.94CLNEE562 pKa = 4.61IYY564 pKa = 10.2PSPLALQEE572 pKa = 4.18HH573 pKa = 6.68YY574 pKa = 9.62KK575 pKa = 10.61VCMLTDD581 pKa = 3.66RR582 pKa = 11.84FTRR585 pKa = 11.84QIKK588 pKa = 8.82EE589 pKa = 3.74KK590 pKa = 10.47IMNVEE595 pKa = 4.51TKK597 pKa = 9.81PHH599 pKa = 5.54SGLYY603 pKa = 9.94KK604 pKa = 10.09RR605 pKa = 11.84LCVFQYY611 pKa = 11.15KK612 pKa = 9.24NRR614 pKa = 11.84CYY616 pKa = 10.55IFTIWIFLLIFQMLVWAASAEE637 pKa = 4.29VITFEE642 pKa = 5.64KK643 pKa = 10.04EE644 pKa = 3.69WNDD647 pKa = 3.24NVHH650 pKa = 6.96GIGSEE655 pKa = 3.95KK656 pKa = 10.75LITDD660 pKa = 4.42LEE662 pKa = 4.66LDD664 pKa = 3.99FSIPSSSGFTYY675 pKa = 9.92TRR677 pKa = 11.84NLEE680 pKa = 4.12GPIPNQKK687 pKa = 9.83LHH689 pKa = 6.44FALKK693 pKa = 10.66LSRR696 pKa = 11.84QKK698 pKa = 9.5TRR700 pKa = 11.84ATIQKK705 pKa = 9.39LGHH708 pKa = 6.01WVDD711 pKa = 3.52ARR713 pKa = 11.84WNVRR717 pKa = 11.84TVFHH721 pKa = 7.23CYY723 pKa = 9.22GACSKK728 pKa = 10.94FEE730 pKa = 4.22YY731 pKa = 8.91PWKK734 pKa = 10.55SATCSRR740 pKa = 11.84EE741 pKa = 3.36KK742 pKa = 10.88DD743 pKa = 3.41FEE745 pKa = 4.35YY746 pKa = 9.64QTGWGCNPGDD756 pKa = 3.99CPGINTGCTACGLYY770 pKa = 10.18LDD772 pKa = 4.82KK773 pKa = 10.53PRR775 pKa = 11.84SIATVIKK782 pKa = 10.34LIQLDD787 pKa = 4.04YY788 pKa = 10.87EE789 pKa = 4.41RR790 pKa = 11.84EE791 pKa = 3.96VCIQIGTHH799 pKa = 5.46SEE801 pKa = 4.16CKK803 pKa = 10.37KK804 pKa = 9.65ITGNDD809 pKa = 3.49CLSTHH814 pKa = 5.79GVKK817 pKa = 9.68ICLLSTTVKK826 pKa = 9.67LTATDD831 pKa = 3.47TLVFFGPLQQGAVVFKK847 pKa = 10.05NWCTSTCAYY856 pKa = 9.95GDD858 pKa = 4.14PGDD861 pKa = 5.04IMLTEE866 pKa = 4.28NGEE869 pKa = 4.35YY870 pKa = 10.31NCPDD874 pKa = 3.1FTGSFEE880 pKa = 4.12RR881 pKa = 11.84TCRR884 pKa = 11.84FGQTPVCEE892 pKa = 4.09YY893 pKa = 10.44NGNNIGGYY901 pKa = 9.34KK902 pKa = 9.97RR903 pKa = 11.84YY904 pKa = 9.62LATRR908 pKa = 11.84DD909 pKa = 3.44SFFSINMTEE918 pKa = 4.17PIIDD922 pKa = 3.63KK923 pKa = 10.3TKK925 pKa = 11.13LEE927 pKa = 4.14WFDD930 pKa = 4.11PDD932 pKa = 3.55STSRR936 pKa = 11.84DD937 pKa = 3.64HH938 pKa = 8.15INVQVSKK945 pKa = 11.21DD946 pKa = 3.29VDD948 pKa = 4.19FEE950 pKa = 4.44NLGNNPCRR958 pKa = 11.84LTLKK962 pKa = 7.86TTGIEE967 pKa = 4.26GAWGSEE973 pKa = 3.84TGFSLTCKK981 pKa = 10.34ISLLEE986 pKa = 4.3CNSFVTMIKK995 pKa = 10.38ACDD998 pKa = 3.18KK999 pKa = 10.71AMCYY1003 pKa = 9.84GAYY1006 pKa = 8.76STTLQRR1012 pKa = 11.84GDD1014 pKa = 3.65NNVIIQGRR1022 pKa = 11.84GGHH1025 pKa = 6.0SGSSFKK1031 pKa = 10.81CCHH1034 pKa = 6.52NDD1036 pKa = 3.25DD1037 pKa = 4.21CSEE1040 pKa = 4.13TGVLADD1046 pKa = 4.31APHH1049 pKa = 6.97LSRR1052 pKa = 11.84LKK1054 pKa = 10.98SQDD1057 pKa = 2.83GDD1059 pKa = 3.36NSEE1062 pKa = 4.62IYY1064 pKa = 10.57SDD1066 pKa = 3.84GANEE1070 pKa = 4.57CRR1072 pKa = 11.84ITCWFNKK1079 pKa = 7.63TSEE1082 pKa = 4.12WVMGMLSGNWLVILVLVGIMLFSVFLFCFFCPAKK1116 pKa = 9.62THH1118 pKa = 5.42QAA1120 pKa = 3.31

Molecular weight:
126.37 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

3705

436

2149

1235.0

140.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.723 ± 0.456

2.699 ± 1.179

5.668 ± 0.515

6.019 ± 0.676

5.155 ± 0.648

5.479 ± 0.682

2.618 ± 0.541

7.368 ± 0.325

7.152 ± 0.235

9.474 ± 0.54

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.97 ± 0.26

4.534 ± 0.117

3.59 ± 0.29

4.238 ± 0.584

4.022 ± 0.617

7.26 ± 0.219

6.478 ± 0.82

6.046 ± 0.891

1.592 ± 0.288

3.914 ± 0.364

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski