Imjin virus
Average proteome isoelectric point is 6.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A075IK44|A0A075IK44_9VIRU Replicase OS=Imjin virus OX=467989 PE=4 SV=1
MM1 pKa = 6.87 AQSRR5 pKa = 11.84 ITRR8 pKa = 11.84 EE9 pKa = 4.31 DD10 pKa = 3.12 IQKK13 pKa = 10.87 LEE15 pKa = 4.13 DD16 pKa = 3.25 AKK18 pKa = 11.07 KK19 pKa = 10.39 QIEE22 pKa = 4.27 IQLSSSIQKK31 pKa = 10.2 LATEE35 pKa = 4.91 TEE37 pKa = 4.38 QFGPDD42 pKa = 3.8 PDD44 pKa = 5.6 PIQLQAVRR52 pKa = 11.84 QRR54 pKa = 11.84 SSVIEE59 pKa = 4.4 GYY61 pKa = 9.73 KK62 pKa = 10.44 EE63 pKa = 3.74 KK64 pKa = 10.92 LRR66 pKa = 11.84 DD67 pKa = 3.68 LEE69 pKa = 4.43 GQLSIAYY76 pKa = 7.64 ATFADD81 pKa = 4.23 EE82 pKa = 4.21 EE83 pKa = 4.44 EE84 pKa = 4.13 EE85 pKa = 4.04 RR86 pKa = 11.84 KK87 pKa = 9.87 KK88 pKa = 10.73 KK89 pKa = 10.7 RR90 pKa = 11.84 EE91 pKa = 3.84 VPGVEE96 pKa = 3.78 EE97 pKa = 5.05 GDD99 pKa = 3.83 YY100 pKa = 11.11 LSQKK104 pKa = 10.51 SSLRR108 pKa = 11.84 YY109 pKa = 10.4 GNVIDD114 pKa = 5.82 LNPLNLEE121 pKa = 4.1 EE122 pKa = 5.2 PAGQTANWNRR132 pKa = 11.84 IFEE135 pKa = 4.6 YY136 pKa = 10.11 IATITQVLLLKK147 pKa = 10.48 GLYY150 pKa = 9.91 ILTTRR155 pKa = 11.84 GRR157 pKa = 11.84 QTSKK161 pKa = 10.99 DD162 pKa = 3.61 NKK164 pKa = 7.3 GTRR167 pKa = 11.84 MKK169 pKa = 10.89 LKK171 pKa = 10.31 DD172 pKa = 4.19 DD173 pKa = 3.86 SCMEE177 pKa = 4.57 TINGIQRR184 pKa = 11.84 HH185 pKa = 4.93 KK186 pKa = 10.49 FLYY189 pKa = 10.03 ISLPTSQSSIQDD201 pKa = 3.63 DD202 pKa = 4.15 EE203 pKa = 4.38 LTPGRR208 pKa = 11.84 FRR210 pKa = 11.84 TIVSGLLPNEE220 pKa = 4.18 IKK222 pKa = 10.51 SKK224 pKa = 11.03 KK225 pKa = 10.11 LMSPVMGVIGFLNLTEE241 pKa = 3.85 VWMDD245 pKa = 3.68 IIDD248 pKa = 5.36 RR249 pKa = 11.84 IMTDD253 pKa = 2.47 QCDD256 pKa = 3.55 YY257 pKa = 8.19 MTQDD261 pKa = 3.57 KK262 pKa = 11.07 SNPTASTNRR271 pKa = 11.84 AYY273 pKa = 10.54 FKK275 pKa = 10.58 EE276 pKa = 4.04 RR277 pKa = 11.84 QNTINSLNLPDD288 pKa = 5.17 LKK290 pKa = 10.58 QLRR293 pKa = 11.84 TDD295 pKa = 3.93 AEE297 pKa = 4.33 RR298 pKa = 11.84 KK299 pKa = 9.72 HH300 pKa = 5.93 YY301 pKa = 10.83 LPDD304 pKa = 5.31 DD305 pKa = 3.91 IEE307 pKa = 4.63 TEE309 pKa = 4.21 CVPWIFANAPDD320 pKa = 4.11 RR321 pKa = 11.84 CPPTVLLVAGIPEE334 pKa = 4.66 LGAFFALMQDD344 pKa = 2.92 IRR346 pKa = 11.84 SGILASNLRR355 pKa = 11.84 GTAEE359 pKa = 3.79 EE360 pKa = 4.37 KK361 pKa = 10.25 IARR364 pKa = 11.84 KK365 pKa = 9.81 SSFYY369 pKa = 10.31 QSYY372 pKa = 10.27 IRR374 pKa = 11.84 RR375 pKa = 11.84 TQSMGLNCDD384 pKa = 2.99 QKK386 pKa = 10.76 IIHH389 pKa = 7.06 IYY391 pKa = 9.25 MDD393 pKa = 3.71 YY394 pKa = 11.21 LGTFCVDD401 pKa = 3.59 HH402 pKa = 6.9 FNLGDD407 pKa = 5.38 DD408 pKa = 4.26 MDD410 pKa = 5.66 PDD412 pKa = 4.46 LKK414 pKa = 10.77 IKK416 pKa = 10.53 AQALLDD422 pKa = 3.93 KK423 pKa = 10.22 KK424 pKa = 10.79 VKK426 pKa = 10.2 EE427 pKa = 4.29 ISTQEE432 pKa = 4.13 PIKK435 pKa = 10.86 LL436 pKa = 3.59
Molecular weight: 49.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.457
IPC2_protein 5.575
IPC_protein 5.537
Toseland 5.575
ProMoST 5.728
Dawson 5.588
Bjellqvist 5.69
Wikipedia 5.499
Rodwell 5.525
Grimsley 5.537
Solomon 5.588
Lehninger 5.563
Nozaki 5.753
DTASelect 5.919
Thurlkill 5.664
EMBOSS 5.626
Sillero 5.83
Patrickios 4.393
IPC_peptide 5.601
IPC2_peptide 5.804
IPC2.peptide.svr19 5.972
Protein with the highest isoelectric point:
>tr|A0A075II01|A0A075II01_9VIRU Nucleocapsid protein OS=Imjin virus OX=467989 PE=3 SV=1
MM1 pKa = 7.46 NNGFIIVLLQSLLQVYY17 pKa = 10.0 CSTHH21 pKa = 5.54 SRR23 pKa = 11.84 ITLEE27 pKa = 4.17 CPHH30 pKa = 7.08 YY31 pKa = 10.28 GVSDD35 pKa = 3.85 AEE37 pKa = 4.46 YY38 pKa = 9.21 VWGKK42 pKa = 10.16 VKK44 pKa = 10.43 IGRR47 pKa = 11.84 FTIDD51 pKa = 4.48 DD52 pKa = 3.44 IKK54 pKa = 10.93 HH55 pKa = 5.21 IQFEE59 pKa = 4.59 NTCPFSITGHH69 pKa = 5.32 NVQTGMKK76 pKa = 9.15 IISWTEE82 pKa = 3.8 KK83 pKa = 10.19 EE84 pKa = 4.21 NHH86 pKa = 6.97 DD87 pKa = 3.8 SSAVYY92 pKa = 9.41 FDD94 pKa = 4.33 KK95 pKa = 11.28 VATHH99 pKa = 5.87 SEE101 pKa = 3.88 KK102 pKa = 10.49 TGDD105 pKa = 3.76 FKK107 pKa = 11.43 SLCGSVSYY115 pKa = 8.86 LTYY118 pKa = 9.87 PFKK121 pKa = 11.05 SGSSYY126 pKa = 11.08 CLNLHH131 pKa = 6.86 CNQTVCDD138 pKa = 3.79 TKK140 pKa = 11.22 VIVLIKK146 pKa = 10.19 AISWVAYY153 pKa = 9.0 DD154 pKa = 4.13 SCVLYY159 pKa = 10.92 HH160 pKa = 7.21 KK161 pKa = 10.11 DD162 pKa = 3.59 IKK164 pKa = 10.89 VQLDD168 pKa = 3.74 FKK170 pKa = 10.23 KK171 pKa = 7.54 THH173 pKa = 5.8 CVKK176 pKa = 10.73 SVLHH180 pKa = 6.09 EE181 pKa = 4.4 KK182 pKa = 10.5 KK183 pKa = 10.45 CFQPDD188 pKa = 3.14 FTTAFNPKK196 pKa = 9.36 LHH198 pKa = 6.59 MKK200 pKa = 10.39 FSLPSVCLFMPSDD213 pKa = 3.9 GNAEE217 pKa = 4.02 GTKK220 pKa = 10.09 EE221 pKa = 3.96 IVGQDD226 pKa = 2.96 SCTDD230 pKa = 2.86 IKK232 pKa = 11.36 LNGWYY237 pKa = 9.93 SCIVGHH243 pKa = 6.02 YY244 pKa = 11.07 SMIFVHH250 pKa = 6.73 SNGKK254 pKa = 8.06 MRR256 pKa = 11.84 QIHH259 pKa = 6.8 LNTKK263 pKa = 8.53 FRR265 pKa = 11.84 KK266 pKa = 9.29 NPQGDD271 pKa = 3.59 DD272 pKa = 3.44 HH273 pKa = 8.55 SEE275 pKa = 3.87 LRR277 pKa = 11.84 SGSTHH282 pKa = 6.62 IIGYY286 pKa = 10.49 KK287 pKa = 10.68 NMTTVSNKK295 pKa = 9.66 VNTLAAFKK303 pKa = 10.73 GATDD307 pKa = 3.9 YY308 pKa = 11.8 SSLFYY313 pKa = 10.85 NIQNTDD319 pKa = 3.25 KK320 pKa = 11.15 KK321 pKa = 10.04 PLLLAGYY328 pKa = 7.5 TPTFHH333 pKa = 6.82 WRR335 pKa = 11.84 EE336 pKa = 4.38 CGTHH340 pKa = 7.38 LIPAVWTSHH349 pKa = 5.35 IDD351 pKa = 3.1 VWGTIIEE358 pKa = 4.13 SDD360 pKa = 3.64 EE361 pKa = 4.37 CNIFCTLSGPGASCEE376 pKa = 4.11 AFSPTGIFLLNSTTCLIPHH395 pKa = 5.49 THH397 pKa = 6.41 RR398 pKa = 11.84 FKK400 pKa = 11.42 GLGDD404 pKa = 3.85 QITFICQRR412 pKa = 11.84 VDD414 pKa = 2.86 TDD416 pKa = 5.05 LEE418 pKa = 5.04 FYY420 pKa = 11.17 CNGRR424 pKa = 11.84 HH425 pKa = 6.35 KK426 pKa = 10.52 IIRR429 pKa = 11.84 TKK431 pKa = 9.94 TLIIGQCIQTFTSFFSLFPGVAHH454 pKa = 6.56 SLAVEE459 pKa = 4.11 LCVPGFHH466 pKa = 7.18 GFITVCIVSTFCFGWLWIPGLTWLILQLLKK496 pKa = 10.89 FIVLFVSTNSVDD508 pKa = 3.57 QRR510 pKa = 11.84 FKK512 pKa = 11.25 QILQKK517 pKa = 10.56 IKK519 pKa = 10.54 AEE521 pKa = 3.92 YY522 pKa = 10.05 RR523 pKa = 11.84 LTIGDD528 pKa = 4.32 TSCEE532 pKa = 4.09 FCKK535 pKa = 10.71 HH536 pKa = 5.05 EE537 pKa = 4.59 CQTSLEE543 pKa = 4.26 SDD545 pKa = 3.51 SHH547 pKa = 8.4 ILYY550 pKa = 10.09 CKK552 pKa = 9.88 QGKK555 pKa = 9.1 CPYY558 pKa = 8.94 CLNEE562 pKa = 4.61 IYY564 pKa = 10.2 PSPLALQEE572 pKa = 4.18 HH573 pKa = 6.68 YY574 pKa = 9.62 KK575 pKa = 10.61 VCMLTDD581 pKa = 3.66 RR582 pKa = 11.84 FTRR585 pKa = 11.84 QIKK588 pKa = 8.82 EE589 pKa = 3.74 KK590 pKa = 10.47 IMNVEE595 pKa = 4.51 TKK597 pKa = 9.81 PHH599 pKa = 5.54 SGLYY603 pKa = 9.94 KK604 pKa = 10.09 RR605 pKa = 11.84 LCVFQYY611 pKa = 11.15 KK612 pKa = 9.24 NRR614 pKa = 11.84 CYY616 pKa = 10.55 IFTIWIFLLIFQMLVWAASAEE637 pKa = 4.29 VITFEE642 pKa = 5.64 KK643 pKa = 10.04 EE644 pKa = 3.69 WNDD647 pKa = 3.24 NVHH650 pKa = 6.96 GIGSEE655 pKa = 3.95 KK656 pKa = 10.75 LITDD660 pKa = 4.42 LEE662 pKa = 4.66 LDD664 pKa = 3.99 FSIPSSSGFTYY675 pKa = 9.92 TRR677 pKa = 11.84 NLEE680 pKa = 4.12 GPIPNQKK687 pKa = 9.83 LHH689 pKa = 6.44 FALKK693 pKa = 10.66 LSRR696 pKa = 11.84 QKK698 pKa = 9.5 TRR700 pKa = 11.84 ATIQKK705 pKa = 9.39 LGHH708 pKa = 6.01 WVDD711 pKa = 3.52 ARR713 pKa = 11.84 WNVRR717 pKa = 11.84 TVFHH721 pKa = 7.23 CYY723 pKa = 9.22 GACSKK728 pKa = 10.94 FEE730 pKa = 4.22 YY731 pKa = 8.91 PWKK734 pKa = 10.55 SATCSRR740 pKa = 11.84 EE741 pKa = 3.36 KK742 pKa = 10.88 DD743 pKa = 3.41 FEE745 pKa = 4.35 YY746 pKa = 9.64 QTGWGCNPGDD756 pKa = 3.99 CPGINTGCTACGLYY770 pKa = 10.18 LDD772 pKa = 4.82 KK773 pKa = 10.53 PRR775 pKa = 11.84 SIATVIKK782 pKa = 10.34 LIQLDD787 pKa = 4.04 YY788 pKa = 10.87 EE789 pKa = 4.41 RR790 pKa = 11.84 EE791 pKa = 3.96 VCIQIGTHH799 pKa = 5.46 SEE801 pKa = 4.16 CKK803 pKa = 10.37 KK804 pKa = 9.65 ITGNDD809 pKa = 3.49 CLSTHH814 pKa = 5.79 GVKK817 pKa = 9.68 ICLLSTTVKK826 pKa = 9.67 LTATDD831 pKa = 3.47 TLVFFGPLQQGAVVFKK847 pKa = 10.05 NWCTSTCAYY856 pKa = 9.95 GDD858 pKa = 4.14 PGDD861 pKa = 5.04 IMLTEE866 pKa = 4.28 NGEE869 pKa = 4.35 YY870 pKa = 10.31 NCPDD874 pKa = 3.1 FTGSFEE880 pKa = 4.12 RR881 pKa = 11.84 TCRR884 pKa = 11.84 FGQTPVCEE892 pKa = 4.09 YY893 pKa = 10.44 NGNNIGGYY901 pKa = 9.34 KK902 pKa = 9.97 RR903 pKa = 11.84 YY904 pKa = 9.62 LATRR908 pKa = 11.84 DD909 pKa = 3.44 SFFSINMTEE918 pKa = 4.17 PIIDD922 pKa = 3.63 KK923 pKa = 10.3 TKK925 pKa = 11.13 LEE927 pKa = 4.14 WFDD930 pKa = 4.11 PDD932 pKa = 3.55 STSRR936 pKa = 11.84 DD937 pKa = 3.64 HH938 pKa = 8.15 INVQVSKK945 pKa = 11.21 DD946 pKa = 3.29 VDD948 pKa = 4.19 FEE950 pKa = 4.44 NLGNNPCRR958 pKa = 11.84 LTLKK962 pKa = 7.86 TTGIEE967 pKa = 4.26 GAWGSEE973 pKa = 3.84 TGFSLTCKK981 pKa = 10.34 ISLLEE986 pKa = 4.3 CNSFVTMIKK995 pKa = 10.38 ACDD998 pKa = 3.18 KK999 pKa = 10.71 AMCYY1003 pKa = 9.84 GAYY1006 pKa = 8.76 STTLQRR1012 pKa = 11.84 GDD1014 pKa = 3.65 NNVIIQGRR1022 pKa = 11.84 GGHH1025 pKa = 6.0 SGSSFKK1031 pKa = 10.81 CCHH1034 pKa = 6.52 NDD1036 pKa = 3.25 DD1037 pKa = 4.21 CSEE1040 pKa = 4.13 TGVLADD1046 pKa = 4.31 APHH1049 pKa = 6.97 LSRR1052 pKa = 11.84 LKK1054 pKa = 10.98 SQDD1057 pKa = 2.83 GDD1059 pKa = 3.36 NSEE1062 pKa = 4.62 IYY1064 pKa = 10.57 SDD1066 pKa = 3.84 GANEE1070 pKa = 4.57 CRR1072 pKa = 11.84 ITCWFNKK1079 pKa = 7.63 TSEE1082 pKa = 4.12 WVMGMLSGNWLVILVLVGIMLFSVFLFCFFCPAKK1116 pKa = 9.62 THH1118 pKa = 5.42 QAA1120 pKa = 3.31
Molecular weight: 126.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.641
IPC2_protein 6.766
IPC_protein 6.634
Toseland 6.517
ProMoST 7.234
Dawson 7.249
Bjellqvist 7.644
Wikipedia 7.176
Rodwell 7.263
Grimsley 6.59
Solomon 7.249
Lehninger 7.278
Nozaki 7.995
DTASelect 7.527
Thurlkill 7.571
EMBOSS 7.541
Sillero 7.819
Patrickios 3.223
IPC_peptide 7.263
IPC2_peptide 7.22
IPC2.peptide.svr19 6.969
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
3705
436
2149
1235.0
140.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.723 ± 0.456
2.699 ± 1.179
5.668 ± 0.515
6.019 ± 0.676
5.155 ± 0.648
5.479 ± 0.682
2.618 ± 0.541
7.368 ± 0.325
7.152 ± 0.235
9.474 ± 0.54
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.97 ± 0.26
4.534 ± 0.117
3.59 ± 0.29
4.238 ± 0.584
4.022 ± 0.617
7.26 ± 0.219
6.478 ± 0.82
6.046 ± 0.891
1.592 ± 0.288
3.914 ± 0.364
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here