Hymenobacter daecheongensis DSM 21074
Average proteome isoelectric point is 7.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3657 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M6I3H0|A0A1M6I3H0_9BACT Uncharacterized protein OS=Hymenobacter daecheongensis DSM 21074 OX=1121955 GN=SAMN02745146_2777 PE=4 SV=1
MM1 pKa = 7.8 DD2 pKa = 4.78 TLTTAPVALDD12 pKa = 3.72 SPAAPAATPGITPGQQTFIQNMQDD36 pKa = 3.25 QVGKK40 pKa = 10.14 FLSTKK45 pKa = 10.45 LDD47 pKa = 3.66 GPFQAFNYY55 pKa = 9.51 PSGFHH60 pKa = 6.06 KK61 pKa = 10.93 VLMVGNDD68 pKa = 3.52 GYY70 pKa = 11.62 YY71 pKa = 10.58 NPRR74 pKa = 11.84 TLLDD78 pKa = 3.08 IDD80 pKa = 4.0 KK81 pKa = 10.72 LVDD84 pKa = 3.21 EE85 pKa = 4.97 GQPLRR90 pKa = 11.84 LDD92 pKa = 3.37 PTATFSGMYY101 pKa = 10.38 AEE103 pKa = 5.93 LINGAKK109 pKa = 10.0 FQFSKK114 pKa = 11.34 ADD116 pKa = 3.35 AEE118 pKa = 4.82 LIAQEE123 pKa = 4.45 EE124 pKa = 4.48 GDD126 pKa = 3.95 AQEE129 pKa = 4.74 VIPAILSAFTSEE141 pKa = 5.18 VEE143 pKa = 4.37 DD144 pKa = 5.3 FPAGTTNKK152 pKa = 9.8 VGYY155 pKa = 9.85 VYY157 pKa = 11.15 AWLQTNYY164 pKa = 10.91 AGNTDD169 pKa = 4.55 NLPPMYY175 pKa = 10.46 SEE177 pKa = 4.53 LQNALGTYY185 pKa = 7.89 EE186 pKa = 4.23 SKK188 pKa = 10.88 AVNSIRR194 pKa = 11.84 LNKK197 pKa = 9.41 EE198 pKa = 3.39 LNDD201 pKa = 3.24 AMTRR205 pKa = 11.84 LAAAAANVTNPSAANGGLQTGATTWYY231 pKa = 8.62 PAYY234 pKa = 10.45 SLPDD238 pKa = 3.52 AQALSGSLEE247 pKa = 4.19 TATNAVDD254 pKa = 3.28 VHH256 pKa = 6.13 LAISDD261 pKa = 4.26 FSSSSSQVSMSGYY274 pKa = 10.24 GSFGMGIADD283 pKa = 3.19 IFDD286 pKa = 4.05 FGGSGDD292 pKa = 3.61 AQYY295 pKa = 11.53 NSSQFSSSDD304 pKa = 2.9 AAMTIDD310 pKa = 3.21 INYY313 pKa = 9.34 PGVTTFSVSPVALSADD329 pKa = 3.96 NKK331 pKa = 9.11 TGWYY335 pKa = 9.99 DD336 pKa = 3.28 HH337 pKa = 7.47 DD338 pKa = 3.85 IVEE341 pKa = 4.7 GLMNNSGQDD350 pKa = 3.16 KK351 pKa = 9.98 TGYY354 pKa = 10.97 ALISTTQYY362 pKa = 10.61 PVAEE366 pKa = 4.08 YY367 pKa = 10.3 FGTGKK372 pKa = 10.48 AFAHH376 pKa = 6.08 MRR378 pKa = 11.84 TFVLSQQPTITLTLSGSNSSQLSKK402 pKa = 11.29 DD403 pKa = 3.11 LTANANTEE411 pKa = 3.88 VDD413 pKa = 3.19 IMGLFHH419 pKa = 7.23 VGGSGSYY426 pKa = 7.13 QTHH429 pKa = 6.74 SIDD432 pKa = 3.71 DD433 pKa = 3.85 TSSQGSVTVTFGPTPDD449 pKa = 3.8 GSDD452 pKa = 3.17 PAGQVAYY459 pKa = 11.29 VMGGVVSYY467 pKa = 10.3 PPASS471 pKa = 3.28
Molecular weight: 49.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.814
IPC2_protein 4.037
IPC_protein 4.05
Toseland 3.834
ProMoST 4.215
Dawson 4.05
Bjellqvist 4.202
Wikipedia 3.986
Rodwell 3.884
Grimsley 3.745
Solomon 4.037
Lehninger 3.999
Nozaki 4.151
DTASelect 4.418
Thurlkill 3.884
EMBOSS 3.986
Sillero 4.177
Patrickios 1.087
IPC_peptide 4.037
IPC2_peptide 4.151
IPC2.peptide.svr19 4.044
Protein with the highest isoelectric point:
>tr|A0A1M6KTN8|A0A1M6KTN8_9BACT Por secretion system C-terminal sorting domain-containing protein OS=Hymenobacter daecheongensis DSM 21074 OX=1121955 GN=SAMN02745146_3563 PE=4 SV=1
MM1 pKa = 7.42 NAPASSPFTLARR13 pKa = 11.84 TCVFRR18 pKa = 11.84 RR19 pKa = 11.84 YY20 pKa = 10.64 LMLRR24 pKa = 11.84 HH25 pKa = 5.06 YY26 pKa = 8.52 TAAPLAVLVAKK37 pKa = 10.46 AIRR40 pKa = 11.84 RR41 pKa = 11.84 AARR44 pKa = 11.84 KK45 pKa = 8.8 RR46 pKa = 11.84 QKK48 pKa = 10.4 QEE50 pKa = 3.66 AKK52 pKa = 10.11 SEE54 pKa = 4.14 RR55 pKa = 11.84 LRR57 pKa = 11.84 DD58 pKa = 4.12 FYY60 pKa = 11.33 GWCAGCEE67 pKa = 4.05 AYY69 pKa = 10.39 GSIFF73 pKa = 3.46
Molecular weight: 8.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.339
IPC2_protein 9.809
IPC_protein 10.643
Toseland 10.511
ProMoST 10.292
Dawson 10.672
Bjellqvist 10.438
Wikipedia 10.906
Rodwell 10.847
Grimsley 10.745
Solomon 10.774
Lehninger 10.73
Nozaki 10.54
DTASelect 10.423
Thurlkill 10.555
EMBOSS 10.921
Sillero 10.613
Patrickios 10.599
IPC_peptide 10.76
IPC2_peptide 9.692
IPC2.peptide.svr19 8.301
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3657
0
3657
1260074
23
3238
344.6
37.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.832 ± 0.058
0.701 ± 0.012
4.872 ± 0.032
5.237 ± 0.045
4.125 ± 0.026
7.637 ± 0.044
2.037 ± 0.022
4.423 ± 0.035
3.993 ± 0.046
11.07 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.886 ± 0.018
3.708 ± 0.03
5.298 ± 0.03
4.59 ± 0.034
6.251 ± 0.036
5.43 ± 0.036
6.362 ± 0.061
7.005 ± 0.032
1.156 ± 0.016
3.388 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here