Desulfobacterium vacuolatum DSM 3385

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfobacterales; Desulfobacteraceae; Desulfobacterium; Desulfobacterium vacuolatum

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4083 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W1YN33|A0A1W1YN33_9DELT Acetoin utilization protein AcuB OS=Desulfobacterium vacuolatum DSM 3385 OX=1121400 GN=SAMN02746065_101204 PE=4 SV=1
MM1 pKa = 7.56KK2 pKa = 10.68KK3 pKa = 8.27LTRR6 pKa = 11.84YY7 pKa = 9.09IAIAVTCLFFVAAGANAMTFKK28 pKa = 10.74AGDD31 pKa = 3.59MGEE34 pKa = 4.15MQINAAFKK42 pKa = 10.53ASFGATQYY50 pKa = 11.72LDD52 pKa = 4.35FVDD55 pKa = 5.85DD56 pKa = 3.92YY57 pKa = 12.07SKK59 pKa = 11.79GDD61 pKa = 4.02DD62 pKa = 3.67SDD64 pKa = 4.89FDD66 pKa = 3.74ATRR69 pKa = 11.84EE70 pKa = 4.01LSFKK74 pKa = 10.61AQWILNKK81 pKa = 9.82NLKK84 pKa = 10.3GVVSFQIGEE93 pKa = 4.31GSTGGYY99 pKa = 9.76FGSTDD104 pKa = 3.21ALVGGEE110 pKa = 4.14EE111 pKa = 4.42DD112 pKa = 3.7GDD114 pKa = 5.05LILEE118 pKa = 4.74LDD120 pKa = 3.54NLYY123 pKa = 10.18IDD125 pKa = 4.52FTTDD129 pKa = 2.35SGINFKK135 pKa = 10.42IGSQGFSLAEE145 pKa = 3.47IAYY148 pKa = 9.36GSNLFYY154 pKa = 10.47EE155 pKa = 5.03VPAGVTFSAPLTNSSSLQAGWFRR178 pKa = 11.84MADD181 pKa = 3.51LMDD184 pKa = 5.27DD185 pKa = 4.17SNSNTDD191 pKa = 3.34DD192 pKa = 3.54QADD195 pKa = 3.64FLYY198 pKa = 10.96AKK200 pKa = 10.4LPVKK204 pKa = 9.96MDD206 pKa = 5.3AISFTPWAAYY216 pKa = 10.52ANIQEE221 pKa = 4.63DD222 pKa = 4.68VIANAPSHH230 pKa = 5.25WRR232 pKa = 11.84YY233 pKa = 10.45AYY235 pKa = 10.2FDD237 pKa = 3.72YY238 pKa = 11.11PGFLTGANSAVIDD251 pKa = 3.84PTATDD256 pKa = 5.46DD257 pKa = 3.64VSAWYY262 pKa = 10.24LGVSFGYY269 pKa = 9.62TMDD272 pKa = 4.21ALSIQASATYY282 pKa = 11.08GDD284 pKa = 4.69MDD286 pKa = 4.03WEE288 pKa = 4.42TATVDD293 pKa = 3.62ASIAGYY299 pKa = 9.29FADD302 pKa = 5.56LVIDD306 pKa = 3.69YY307 pKa = 10.67KK308 pKa = 10.64MDD310 pKa = 3.21GFTPEE315 pKa = 4.27FFAFYY320 pKa = 11.02GNGPDD325 pKa = 5.52ANDD328 pKa = 3.74EE329 pKa = 4.61DD330 pKa = 5.9FDD332 pKa = 4.13MMPVLIGGPTYY343 pKa = 9.7TSSYY347 pKa = 10.28FGGSRR352 pKa = 11.84FNDD355 pKa = 3.34NMFDD359 pKa = 4.34SYY361 pKa = 11.8DD362 pKa = 3.52STYY365 pKa = 9.48ATSMWAVGFKK375 pKa = 10.86VKK377 pKa = 10.4DD378 pKa = 3.19IKK380 pKa = 10.27TGEE383 pKa = 4.22KK384 pKa = 10.05LSHH387 pKa = 6.67EE388 pKa = 4.33FQIMYY393 pKa = 10.77AEE395 pKa = 4.62GTAEE399 pKa = 3.85DD400 pKa = 5.01TIFQSPNDD408 pKa = 3.36ILMNEE413 pKa = 4.39DD414 pKa = 3.38EE415 pKa = 5.54SVLEE419 pKa = 4.06VNFNSEE425 pKa = 4.12YY426 pKa = 10.83QIMKK430 pKa = 9.65HH431 pKa = 5.92LLFATEE437 pKa = 4.53LGYY440 pKa = 7.79MTFDD444 pKa = 3.59EE445 pKa = 5.08DD446 pKa = 3.94SDD448 pKa = 4.31YY449 pKa = 11.94NEE451 pKa = 4.51ALNGSVEE458 pKa = 4.58DD459 pKa = 4.18FWKK462 pKa = 10.24VACAIEE468 pKa = 4.5LSFF471 pKa = 5.5

Molecular weight: 51.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W1ZTE0|A0A1W1ZTE0_9DELT Oxaloacetate decarboxylase gamma chain OS=Desulfobacterium vacuolatum DSM 3385 OX=1121400 GN=SAMN02746065_103163 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84IKK11 pKa = 10.49RR12 pKa = 11.84ARR14 pKa = 11.84KK15 pKa = 9.05HH16 pKa = 5.9GFLKK20 pKa = 10.66RR21 pKa = 11.84MSTAAGRR28 pKa = 11.84RR29 pKa = 11.84IVNSRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.9GRR39 pKa = 11.84KK40 pKa = 9.09KK41 pKa = 9.67LTAA44 pKa = 4.2

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4083

0

4083

1418271

29

3901

347.4

38.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.836 ± 0.032

1.383 ± 0.016

5.498 ± 0.029

6.089 ± 0.033

4.561 ± 0.028

7.407 ± 0.033

2.299 ± 0.018

7.23 ± 0.033

6.538 ± 0.036

9.616 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.192 ± 0.019

4.031 ± 0.02

4.323 ± 0.023

3.316 ± 0.021

4.839 ± 0.027

5.833 ± 0.024

5.477 ± 0.032

6.661 ± 0.034

1.038 ± 0.014

2.834 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski