Cloacibacillus sp. An23

Taxonomy: cellular organisms; Bacteria; Synergistetes; Synergistia; Synergistales; Synergistaceae; Cloacibacillus; unclassified Cloacibacillus

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2713 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y4GCD7|A0A1Y4GCD7_9BACT Uncharacterized protein OS=Cloacibacillus sp. An23 OX=1965591 GN=B5F39_08965 PE=4 SV=1
MM1 pKa = 7.38TMTGRR6 pKa = 11.84TYY8 pKa = 10.97SGLFGYY14 pKa = 9.96IGTGGIIKK22 pKa = 10.33NLTVAEE28 pKa = 4.07ADD30 pKa = 3.21ITASSGNSMGIIAGVNTGDD49 pKa = 4.36IEE51 pKa = 4.43NCTVSGGITTTSEE64 pKa = 3.74EE65 pKa = 5.0GYY67 pKa = 11.15SNASYY72 pKa = 10.83LGGIAGQNEE81 pKa = 4.56SSGKK85 pKa = 8.09ITNCVNRR92 pKa = 11.84ADD94 pKa = 3.66VYY96 pKa = 11.24GYY98 pKa = 10.79DD99 pKa = 3.57FLGGVVGSNNGGTVEE114 pKa = 3.84NCRR117 pKa = 11.84NYY119 pKa = 9.9GTITGIYY126 pKa = 9.18FYY128 pKa = 10.44IGGIAGYY135 pKa = 10.66GRR137 pKa = 11.84GDD139 pKa = 3.55YY140 pKa = 10.78KK141 pKa = 10.17ITNCANEE148 pKa = 4.22GSVSGASVADD158 pKa = 3.65SRR160 pKa = 11.84PQNIGGIVGYY170 pKa = 10.24GGAGSVTNCVNSGKK184 pKa = 10.3VSGSSDD190 pKa = 3.53FVGGIMGQSSGGDD203 pKa = 3.1KK204 pKa = 9.47ITNCVSTGEE213 pKa = 4.06VSGNGSSYY221 pKa = 9.73VGGIIGHH228 pKa = 6.17TGGATLGNCAWLEE241 pKa = 4.07GTAGKK246 pKa = 10.55GVGGGTQPGGVTMIGTSADD265 pKa = 3.23VSGIATTLSASIDD278 pKa = 3.38KK279 pKa = 9.37TAINPNAATGDD290 pKa = 3.6ADD292 pKa = 3.8YY293 pKa = 11.15SATISLVPFPGKK305 pKa = 10.24LADD308 pKa = 3.65VYY310 pKa = 10.68GTGVKK315 pKa = 10.3DD316 pKa = 4.48AGATTNSGIINITDD330 pKa = 3.38NGNGTFTVTPTGAASGSAQITVTADD355 pKa = 3.3LYY357 pKa = 11.18KK358 pKa = 10.59YY359 pKa = 10.59DD360 pKa = 4.41FASSSYY366 pKa = 11.11SGTEE370 pKa = 3.55YY371 pKa = 9.96TQYY374 pKa = 10.91TFTLNVIVSDD384 pKa = 4.32IEE386 pKa = 4.04LTQITLSPASDD397 pKa = 3.73DD398 pKa = 3.43VSMKK402 pKa = 10.28IGEE405 pKa = 4.34TQTFSVSYY413 pKa = 10.48KK414 pKa = 8.8PTNATNKK421 pKa = 9.53SVTWSASPSGVVSITDD437 pKa = 3.56SGNGAATVAAVAAGEE452 pKa = 4.33TTLTVTSQANSALTDD467 pKa = 3.34TCRR470 pKa = 11.84ITVTPVYY477 pKa = 10.32AEE479 pKa = 4.26NVTISPDD486 pKa = 3.47VTEE489 pKa = 4.1TLYY492 pKa = 11.33LDD494 pKa = 4.5GSSHH498 pKa = 6.42TFTATVSPANAANRR512 pKa = 11.84NVTWSLTDD520 pKa = 3.08VSGTLVQNGDD530 pKa = 3.34VSISGGATDD539 pKa = 4.36NPVTVSTKK547 pKa = 9.61NAASAGSVKK556 pKa = 9.66LTATAAGGDD565 pKa = 4.04PQSGQPSGEE574 pKa = 3.99LTLDD578 pKa = 3.69FALPNVSGLALNAEE592 pKa = 4.41TLDD595 pKa = 3.12IDD597 pKa = 5.12LNVAAYY603 pKa = 10.24GEE605 pKa = 4.57LEE607 pKa = 4.25AKK609 pKa = 10.23VSPSNAQFSAVTWSVEE625 pKa = 3.76PPIASISYY633 pKa = 10.71DD634 pKa = 3.05GDD636 pKa = 3.43KK637 pKa = 8.94QTKK640 pKa = 7.35VTITPSKK647 pKa = 10.63GGTATVTASVGGYY660 pKa = 10.1SDD662 pKa = 3.56TCALTVTPIYY672 pKa = 10.22ATGLTLSSGSLSLEE686 pKa = 4.23SGTTAALTAAVTPDD700 pKa = 3.09NATDD704 pKa = 4.4RR705 pKa = 11.84SVTWTTSDD713 pKa = 3.3AAVASVDD720 pKa = 3.57EE721 pKa = 4.66NGTVSAHH728 pKa = 6.81DD729 pKa = 4.47AGSATITATANGARR743 pKa = 11.84EE744 pKa = 4.14GAEE747 pKa = 3.68LTARR751 pKa = 11.84CIVTVTLPPVPVEE764 pKa = 5.43DD765 pKa = 4.06IDD767 pKa = 3.94IDD769 pKa = 4.06ASGSTALTVGGTVKK783 pKa = 9.6FTATVTPPNATNPAVTWASSDD804 pKa = 3.53EE805 pKa = 4.4SVATVDD811 pKa = 4.55AEE813 pKa = 4.42GLVTARR819 pKa = 11.84GEE821 pKa = 4.24GTATITATSIDD832 pKa = 3.69GSVAASVTVTVAAAEE847 pKa = 4.23PAPAPQPSGGGGGGCSAGWGALALLAIVPLAMKK880 pKa = 10.16RR881 pKa = 11.84RR882 pKa = 11.84KK883 pKa = 9.38

Molecular weight: 87.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y4GHC6|A0A1Y4GHC6_9BACT Hydrolase_4 domain-containing protein OS=Cloacibacillus sp. An23 OX=1965591 GN=B5F39_12855 PE=4 SV=1
MM1 pKa = 7.53TGGISQRR8 pKa = 11.84RR9 pKa = 11.84GANTARR15 pKa = 11.84KK16 pKa = 9.12APPGVLRR23 pKa = 11.84HH24 pKa = 5.62APARR28 pKa = 11.84RR29 pKa = 11.84NIPKK33 pKa = 9.71RR34 pKa = 11.84RR35 pKa = 11.84LRR37 pKa = 11.84AGRR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84SPNEE46 pKa = 3.47SAPFRR51 pKa = 11.84ALSRR55 pKa = 11.84AGSATVV61 pKa = 2.97

Molecular weight: 6.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2713

0

2713

847574

34

3283

312.4

34.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.615 ± 0.063

1.593 ± 0.022

5.178 ± 0.041

7.03 ± 0.06

4.075 ± 0.036

8.549 ± 0.062

1.602 ± 0.022

6.079 ± 0.043

5.231 ± 0.045

9.233 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.945 ± 0.025

3.197 ± 0.038

4.277 ± 0.034

2.268 ± 0.02

6.04 ± 0.051

5.739 ± 0.035

4.886 ± 0.037

7.146 ± 0.035

1.159 ± 0.016

3.16 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski