Amino acid dipepetide frequency for Cloacibacillus sp. An23

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.232AlaAla: 15.232 ± 0.228
1.474AlaCys: 1.474 ± 0.045
5.106AlaAsp: 5.106 ± 0.086
8.236AlaGlu: 8.236 ± 0.104
3.843AlaPhe: 3.843 ± 0.069
9.414AlaGly: 9.414 ± 0.138
1.526AlaHis: 1.526 ± 0.046
5.036AlaIle: 5.036 ± 0.09
5.191AlaLys: 5.191 ± 0.085
11.008AlaLeu: 11.008 ± 0.139
3.068AlaMet: 3.068 ± 0.059
2.609AlaAsn: 2.609 ± 0.065
3.997AlaPro: 3.997 ± 0.095
2.768AlaGln: 2.768 ± 0.066
6.205AlaArg: 6.205 ± 0.125
5.831AlaSer: 5.831 ± 0.095
3.412AlaThr: 3.412 ± 0.073
8.548AlaVal: 8.548 ± 0.113
1.051AlaTrp: 1.051 ± 0.036
2.6AlaTyr: 2.6 ± 0.064
0.0AlaXaa: 0.0 ± 0.0
Cys
1.918CysAla: 1.918 ± 0.063
0.34CysCys: 0.34 ± 0.019
0.794CysAsp: 0.794 ± 0.032
0.949CysGlu: 0.949 ± 0.036
0.675CysPhe: 0.675 ± 0.029
2.089CysGly: 2.089 ± 0.056
0.282CysHis: 0.282 ± 0.017
0.786CysIle: 0.786 ± 0.03
0.503CysLys: 0.503 ± 0.026
1.129CysLeu: 1.129 ± 0.036
0.394CysMet: 0.394 ± 0.02
0.395CysAsn: 0.395 ± 0.023
0.74CysPro: 0.74 ± 0.038
0.228CysGln: 0.228 ± 0.017
1.085CysArg: 1.085 ± 0.035
0.979CysSer: 0.979 ± 0.034
0.717CysThr: 0.717 ± 0.029
1.287CysVal: 1.287 ± 0.044
0.208CysTrp: 0.208 ± 0.017
0.428CysTyr: 0.428 ± 0.023
0.0CysXaa: 0.0 ± 0.0
Asp
5.341AspAla: 5.341 ± 0.092
0.719AspCys: 0.719 ± 0.028
2.723AspAsp: 2.723 ± 0.076
4.452AspGlu: 4.452 ± 0.079
2.432AspPhe: 2.432 ± 0.055
5.446AspGly: 5.446 ± 0.1
0.707AspHis: 0.707 ± 0.033
3.643AspIle: 3.643 ± 0.063
2.79AspLys: 2.79 ± 0.059
3.738AspLeu: 3.738 ± 0.074
1.829AspMet: 1.829 ± 0.05
1.52AspAsn: 1.52 ± 0.049
2.092AspPro: 2.092 ± 0.05
0.772AspGln: 0.772 ± 0.034
2.5AspArg: 2.5 ± 0.053
2.366AspSer: 2.366 ± 0.058
2.237AspThr: 2.237 ± 0.055
3.932AspVal: 3.932 ± 0.066
0.676AspTrp: 0.676 ± 0.032
1.869AspTyr: 1.869 ± 0.048
0.0AspXaa: 0.0 ± 0.0
Glu
7.314GluAla: 7.314 ± 0.119
0.913GluCys: 0.913 ± 0.039
3.418GluAsp: 3.418 ± 0.077
5.511GluGlu: 5.511 ± 0.108
2.298GluPhe: 2.298 ± 0.054
4.767GluGly: 4.767 ± 0.082
1.241GluHis: 1.241 ± 0.041
4.88GluIle: 4.88 ± 0.096
4.98GluLys: 4.98 ± 0.098
6.914GluLeu: 6.914 ± 0.119
2.14GluMet: 2.14 ± 0.049
2.84GluAsn: 2.84 ± 0.064
2.416GluPro: 2.416 ± 0.064
1.844GluGln: 1.844 ± 0.047
4.992GluArg: 4.992 ± 0.101
3.276GluSer: 3.276 ± 0.067
3.679GluThr: 3.679 ± 0.073
3.758GluVal: 3.758 ± 0.071
0.826GluTrp: 0.826 ± 0.032
2.291GluTyr: 2.291 ± 0.053
0.0GluXaa: 0.0 ± 0.0
Phe
4.389PheAla: 4.389 ± 0.072
0.853PheCys: 0.853 ± 0.033
2.303PheAsp: 2.303 ± 0.062
2.244PheGlu: 2.244 ± 0.055
1.95PhePhe: 1.95 ± 0.057
3.567PheGly: 3.567 ± 0.067
0.661PheHis: 0.661 ± 0.026
2.651PheIle: 2.651 ± 0.072
1.47PheLys: 1.47 ± 0.046
3.471PheLeu: 3.471 ± 0.068
1.193PheMet: 1.193 ± 0.038
1.163PheAsn: 1.163 ± 0.038
1.776PhePro: 1.776 ± 0.051
0.785PheGln: 0.785 ± 0.031
2.284PheArg: 2.284 ± 0.051
2.884PheSer: 2.884 ± 0.064
2.216PheThr: 2.216 ± 0.06
2.993PheVal: 2.993 ± 0.053
0.532PheTrp: 0.532 ± 0.026
1.362PheTyr: 1.362 ± 0.037
0.0PheXaa: 0.0 ± 0.0
Gly
9.018GlyAla: 9.018 ± 0.129
1.541GlyCys: 1.541 ± 0.055
4.196GlyAsp: 4.196 ± 0.085
5.824GlyGlu: 5.824 ± 0.087
3.488GlyPhe: 3.488 ± 0.058
8.264GlyGly: 8.264 ± 0.168
1.402GlyHis: 1.402 ± 0.039
5.399GlyIle: 5.399 ± 0.099
4.422GlyLys: 4.422 ± 0.074
7.603GlyLeu: 7.603 ± 0.094
2.613GlyMet: 2.613 ± 0.055
2.392GlyAsn: 2.392 ± 0.073
2.302GlyPro: 2.302 ± 0.056
1.822GlyGln: 1.822 ± 0.057
5.299GlyArg: 5.299 ± 0.09
4.637GlySer: 4.637 ± 0.082
4.472GlyThr: 4.472 ± 0.111
6.848GlyVal: 6.848 ± 0.1
1.097GlyTrp: 1.097 ± 0.041
2.85GlyTyr: 2.85 ± 0.072
0.0GlyXaa: 0.0 ± 0.0
His
1.438HisAla: 1.438 ± 0.048
0.304HisCys: 0.304 ± 0.02
0.901HisAsp: 0.901 ± 0.032
1.051HisGlu: 1.051 ± 0.036
0.628HisPhe: 0.628 ± 0.029
1.598HisGly: 1.598 ± 0.049
0.296HisHis: 0.296 ± 0.019
1.126HisIle: 1.126 ± 0.04
0.8HisLys: 0.8 ± 0.029
1.34HisLeu: 1.34 ± 0.039
0.466HisMet: 0.466 ± 0.026
0.562HisAsn: 0.562 ± 0.027
0.969HisPro: 0.969 ± 0.036
0.297HisGln: 0.297 ± 0.018
0.854HisArg: 0.854 ± 0.033
0.857HisSer: 0.857 ± 0.034
0.765HisThr: 0.765 ± 0.035
1.08HisVal: 1.08 ± 0.036
0.152HisTrp: 0.152 ± 0.012
0.54HisTyr: 0.54 ± 0.023
0.0HisXaa: 0.0 ± 0.0
Ile
6.318IleAla: 6.318 ± 0.092
1.075IleCys: 1.075 ± 0.037
3.597IleAsp: 3.597 ± 0.073
3.945IleGlu: 3.945 ± 0.08
2.67IlePhe: 2.67 ± 0.066
4.789IleGly: 4.789 ± 0.095
0.926IleHis: 0.926 ± 0.034
3.783IleIle: 3.783 ± 0.083
3.413IleLys: 3.413 ± 0.061
5.039IleLeu: 5.039 ± 0.101
1.733IleMet: 1.733 ± 0.049
2.055IleAsn: 2.055 ± 0.057
2.966IlePro: 2.966 ± 0.073
1.333IleGln: 1.333 ± 0.041
3.027IleArg: 3.027 ± 0.067
3.879IleSer: 3.879 ± 0.076
3.49IleThr: 3.49 ± 0.081
4.205IleVal: 4.205 ± 0.074
0.582IleTrp: 0.582 ± 0.028
1.963IleTyr: 1.963 ± 0.054
0.0IleXaa: 0.0 ± 0.0
Lys
4.68LysAla: 4.68 ± 0.078
0.683LysCys: 0.683 ± 0.036
2.816LysAsp: 2.816 ± 0.07
4.08LysGlu: 4.08 ± 0.08
1.859LysPhe: 1.859 ± 0.046
3.444LysGly: 3.444 ± 0.069
0.858LysHis: 0.858 ± 0.03
3.82LysIle: 3.82 ± 0.073
3.716LysLys: 3.716 ± 0.085
4.889LysLeu: 4.889 ± 0.096
1.712LysMet: 1.712 ± 0.043
2.268LysAsn: 2.268 ± 0.046
1.968LysPro: 1.968 ± 0.051
1.201LysGln: 1.201 ± 0.039
3.124LysArg: 3.124 ± 0.066
2.804LysSer: 2.804 ± 0.063
2.775LysThr: 2.775 ± 0.061
3.071LysVal: 3.071 ± 0.064
0.583LysTrp: 0.583 ± 0.029
1.954LysTyr: 1.954 ± 0.057
0.0LysXaa: 0.0 ± 0.0
Leu
10.312LeuAla: 10.312 ± 0.122
1.769LeuCys: 1.769 ± 0.051
4.881LeuAsp: 4.881 ± 0.074
5.853LeuGlu: 5.853 ± 0.098
3.912LeuPhe: 3.912 ± 0.075
7.549LeuGly: 7.549 ± 0.093
1.495LeuHis: 1.495 ± 0.041
4.918LeuIle: 4.918 ± 0.088
4.503LeuLys: 4.503 ± 0.083
8.252LeuLeu: 8.252 ± 0.131
2.604LeuMet: 2.604 ± 0.056
2.793LeuAsn: 2.793 ± 0.061
4.498LeuPro: 4.498 ± 0.083
1.806LeuGln: 1.806 ± 0.048
5.965LeuArg: 5.965 ± 0.113
6.346LeuSer: 6.346 ± 0.099
5.025LeuThr: 5.025 ± 0.084
6.055LeuVal: 6.055 ± 0.083
1.031LeuTrp: 1.031 ± 0.039
2.758LeuTyr: 2.758 ± 0.059
0.0LeuXaa: 0.0 ± 0.0
Met
2.95MetAla: 2.95 ± 0.069
0.374MetCys: 0.374 ± 0.023
1.461MetAsp: 1.461 ± 0.037
2.019MetGlu: 2.019 ± 0.057
0.868MetPhe: 0.868 ± 0.042
2.204MetGly: 2.204 ± 0.05
0.425MetHis: 0.425 ± 0.024
1.638MetIle: 1.638 ± 0.051
2.023MetLys: 2.023 ± 0.047
3.025MetLeu: 3.025 ± 0.076
0.833MetMet: 0.833 ± 0.032
1.161MetAsn: 1.161 ± 0.035
1.608MetPro: 1.608 ± 0.048
0.704MetGln: 0.704 ± 0.029
2.023MetArg: 2.023 ± 0.051
1.864MetSer: 1.864 ± 0.049
1.791MetThr: 1.791 ± 0.053
1.554MetVal: 1.554 ± 0.046
0.258MetTrp: 0.258 ± 0.02
0.671MetTyr: 0.671 ± 0.031
0.0MetXaa: 0.0 ± 0.0
Asn
3.066AsnAla: 3.066 ± 0.079
0.513AsnCys: 0.513 ± 0.031
1.615AsnAsp: 1.615 ± 0.047
1.796AsnGlu: 1.796 ± 0.042
1.403AsnPhe: 1.403 ± 0.043
2.968AsnGly: 2.968 ± 0.089
0.488AsnHis: 0.488 ± 0.023
2.449AsnIle: 2.449 ± 0.05
1.536AsnLys: 1.536 ± 0.048
2.842AsnLeu: 2.842 ± 0.064
0.998AsnMet: 0.998 ± 0.038
1.101AsnAsn: 1.101 ± 0.049
1.707AsnPro: 1.707 ± 0.049
0.65AsnGln: 0.65 ± 0.028
1.572AsnArg: 1.572 ± 0.04
1.765AsnSer: 1.765 ± 0.062
1.482AsnThr: 1.482 ± 0.06
2.539AsnVal: 2.539 ± 0.074
0.376AsnTrp: 0.376 ± 0.02
1.097AsnTyr: 1.097 ± 0.039
0.0AsnXaa: 0.0 ± 0.0
Pro
4.149ProAla: 4.149 ± 0.081
0.584ProCys: 0.584 ± 0.023
2.623ProAsp: 2.623 ± 0.062
4.006ProGlu: 4.006 ± 0.077
1.838ProPhe: 1.838 ± 0.053
3.516ProGly: 3.516 ± 0.075
0.773ProHis: 0.773 ± 0.029
1.935ProIle: 1.935 ± 0.046
1.983ProLys: 1.983 ± 0.051
4.106ProLeu: 4.106 ± 0.073
0.978ProMet: 0.978 ± 0.035
1.219ProAsn: 1.219 ± 0.036
1.675ProPro: 1.675 ± 0.061
1.226ProGln: 1.226 ± 0.041
2.307ProArg: 2.307 ± 0.06
2.478ProSer: 2.478 ± 0.062
1.786ProThr: 1.786 ± 0.047
3.599ProVal: 3.599 ± 0.067
0.552ProTrp: 0.552 ± 0.025
1.437ProTyr: 1.437 ± 0.042
0.0ProXaa: 0.0 ± 0.0
Gln
2.172GlnAla: 2.172 ± 0.056
0.298GlnCys: 0.298 ± 0.021
1.013GlnAsp: 1.013 ± 0.035
1.471GlnGlu: 1.471 ± 0.049
0.848GlnPhe: 0.848 ± 0.029
1.54GlnGly: 1.54 ± 0.044
0.363GlnHis: 0.363 ± 0.019
1.613GlnIle: 1.613 ± 0.055
1.492GlnLys: 1.492 ± 0.053
2.091GlnLeu: 2.091 ± 0.047
0.829GlnMet: 0.829 ± 0.033
0.958GlnAsn: 0.958 ± 0.036
0.967GlnPro: 0.967 ± 0.04
0.759GlnGln: 0.759 ± 0.033
1.424GlnArg: 1.424 ± 0.049
1.274GlnSer: 1.274 ± 0.04
1.2GlnThr: 1.2 ± 0.043
1.281GlnVal: 1.281 ± 0.04
0.277GlnTrp: 0.277 ± 0.017
0.803GlnTyr: 0.803 ± 0.034
0.0GlnXaa: 0.0 ± 0.0
Arg
6.227ArgAla: 6.227 ± 0.118
0.963ArgCys: 0.963 ± 0.041
3.117ArgAsp: 3.117 ± 0.071
4.949ArgGlu: 4.949 ± 0.103
2.481ArgPhe: 2.481 ± 0.06
5.247ArgGly: 5.247 ± 0.092
1.012ArgHis: 1.012 ± 0.036
3.508ArgIle: 3.508 ± 0.068
2.819ArgLys: 2.819 ± 0.057
5.621ArgLeu: 5.621 ± 0.085
1.803ArgMet: 1.803 ± 0.057
1.684ArgAsn: 1.684 ± 0.046
2.387ArgPro: 2.387 ± 0.057
1.37ArgGln: 1.37 ± 0.047
4.941ArgArg: 4.941 ± 0.115
2.952ArgSer: 2.952 ± 0.076
2.57ArgThr: 2.57 ± 0.054
3.99ArgVal: 3.99 ± 0.069
0.744ArgTrp: 0.744 ± 0.032
2.015ArgTyr: 2.015 ± 0.05
0.0ArgXaa: 0.0 ± 0.0
Ser
5.969SerAla: 5.969 ± 0.104
0.949SerCys: 0.949 ± 0.036
2.793SerAsp: 2.793 ± 0.061
3.609SerGlu: 3.609 ± 0.07
2.71SerPhe: 2.71 ± 0.06
5.841SerGly: 5.841 ± 0.108
0.908SerHis: 0.908 ± 0.032
3.16SerIle: 3.16 ± 0.073
2.413SerLys: 2.413 ± 0.049
5.592SerLeu: 5.592 ± 0.09
1.631SerMet: 1.631 ± 0.052
1.567SerAsn: 1.567 ± 0.048
2.617SerPro: 2.617 ± 0.06
1.273SerGln: 1.273 ± 0.042
3.422SerArg: 3.422 ± 0.075
3.35SerSer: 3.35 ± 0.074
2.355SerThr: 2.355 ± 0.055
4.68SerVal: 4.68 ± 0.082
0.713SerTrp: 0.713 ± 0.032
1.837SerTyr: 1.837 ± 0.046
0.0SerXaa: 0.0 ± 0.0
Thr
5.355ThrAla: 5.355 ± 0.075
0.562ThrCys: 0.562 ± 0.027
2.58ThrAsp: 2.58 ± 0.059
3.194ThrGlu: 3.194 ± 0.058
1.863ThrPhe: 1.863 ± 0.053
4.626ThrGly: 4.626 ± 0.09
0.822ThrHis: 0.822 ± 0.029
2.911ThrIle: 2.911 ± 0.058
2.21ThrLys: 2.21 ± 0.057
5.0ThrLeu: 5.0 ± 0.081
1.231ThrMet: 1.231 ± 0.04
1.446ThrAsn: 1.446 ± 0.071
2.878ThrPro: 2.878 ± 0.059
1.222ThrGln: 1.222 ± 0.035
2.369ThrArg: 2.369 ± 0.061
2.407ThrSer: 2.407 ± 0.057
2.248ThrThr: 2.248 ± 0.062
3.994ThrVal: 3.994 ± 0.078
0.517ThrTrp: 0.517 ± 0.029
1.428ThrTyr: 1.428 ± 0.048
0.0ThrXaa: 0.0 ± 0.0
Val
6.365ValAla: 6.365 ± 0.103
1.332ValCys: 1.332 ± 0.043
3.314ValAsp: 3.314 ± 0.064
4.351ValGlu: 4.351 ± 0.074
3.092ValPhe: 3.092 ± 0.066
4.749ValGly: 4.749 ± 0.113
1.084ValHis: 1.084 ± 0.035
4.907ValIle: 4.907 ± 0.091
3.8ValLys: 3.8 ± 0.076
6.692ValLeu: 6.692 ± 0.102
2.222ValMet: 2.222 ± 0.057
2.487ValAsn: 2.487 ± 0.065
3.462ValPro: 3.462 ± 0.061
1.562ValGln: 1.562 ± 0.04
4.278ValArg: 4.278 ± 0.068
4.941ValSer: 4.941 ± 0.082
4.374ValThr: 4.374 ± 0.088
5.438ValVal: 5.438 ± 0.094
0.822ValTrp: 0.822 ± 0.032
2.19ValTyr: 2.19 ± 0.05
0.0ValXaa: 0.0 ± 0.0
Trp
0.952TrpAla: 0.952 ± 0.034
0.162TrpCys: 0.162 ± 0.013
0.648TrpAsp: 0.648 ± 0.033
0.732TrpGlu: 0.732 ± 0.034
0.473TrpPhe: 0.473 ± 0.029
0.925TrpGly: 0.925 ± 0.032
0.269TrpHis: 0.269 ± 0.019
0.643TrpIle: 0.643 ± 0.026
0.681TrpLys: 0.681 ± 0.028
1.22TrpLeu: 1.22 ± 0.044
0.324TrpMet: 0.324 ± 0.022
0.525TrpAsn: 0.525 ± 0.028
0.405TrpPro: 0.405 ± 0.02
0.382TrpGln: 0.382 ± 0.023
0.852TrpArg: 0.852 ± 0.035
0.636TrpSer: 0.636 ± 0.028
0.591TrpThr: 0.591 ± 0.026
0.592TrpVal: 0.592 ± 0.029
0.203TrpTrp: 0.203 ± 0.016
0.374TrpTyr: 0.374 ± 0.023
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.987TyrAla: 2.987 ± 0.057
0.52TyrCys: 0.52 ± 0.026
1.881TyrAsp: 1.881 ± 0.051
2.027TyrGlu: 2.027 ± 0.053
1.413TyrPhe: 1.413 ± 0.04
2.944TyrGly: 2.944 ± 0.06
0.485TyrHis: 0.485 ± 0.027
1.987TyrIle: 1.987 ± 0.053
1.56TyrLys: 1.56 ± 0.046
2.754TyrLeu: 2.754 ± 0.058
0.854TyrMet: 0.854 ± 0.028
1.222TyrAsn: 1.222 ± 0.053
1.34TyrPro: 1.34 ± 0.042
0.673TyrGln: 0.673 ± 0.03
1.876TyrArg: 1.876 ± 0.048
1.862TyrSer: 1.862 ± 0.051
1.679TyrThr: 1.679 ± 0.048
2.019TyrVal: 2.019 ± 0.048
0.387TyrTrp: 0.387 ± 0.021
1.128TyrTyr: 1.128 ± 0.04
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2713 proteins (847574 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski