Butyrivibrio sp. ob235

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Butyrivibrio; unclassified Butyrivibrio

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4267 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H7P0W4|A0A1H7P0W4_9FIRM Uncharacterized protein OS=Butyrivibrio sp. ob235 OX=1761780 GN=SAMN04487770_10889 PE=4 SV=1
MM1 pKa = 7.73KK2 pKa = 10.18KK3 pKa = 10.24RR4 pKa = 11.84LFILIAACLIMSGCGNSTSTEE25 pKa = 4.0RR26 pKa = 11.84PALPTPSEE34 pKa = 4.4TEE36 pKa = 4.19TVSDD40 pKa = 3.66QSADD44 pKa = 3.47EE45 pKa = 4.43TPTMNATDD53 pKa = 4.67DD54 pKa = 4.58GSDD57 pKa = 3.36SSAPASDD64 pKa = 4.7FAQAPSSDD72 pKa = 3.53NASEE76 pKa = 4.65DD77 pKa = 3.67FGDD80 pKa = 3.79TGSPSTSFEE89 pKa = 3.78SDD91 pKa = 3.25APDD94 pKa = 3.18MSEE97 pKa = 4.41PEE99 pKa = 4.49SIGDD103 pKa = 3.7DD104 pKa = 3.59SSPSSSGVATPSGQSPSGVEE124 pKa = 4.08SNFDD128 pKa = 3.44NDD130 pKa = 2.54IDD132 pKa = 4.16EE133 pKa = 4.98PVTASEE139 pKa = 4.89NEE141 pKa = 4.28TEE143 pKa = 4.01SDD145 pKa = 3.29EE146 pKa = 4.6AGSEE150 pKa = 4.11KK151 pKa = 10.89EE152 pKa = 3.78EE153 pKa = 3.95AAPAEE158 pKa = 4.24EE159 pKa = 4.53AQNEE163 pKa = 4.27AQNEE167 pKa = 4.21ATEE170 pKa = 4.25ATSEE174 pKa = 4.18EE175 pKa = 4.33EE176 pKa = 4.34TPKK179 pKa = 10.95EE180 pKa = 3.72EE181 pKa = 3.94STGYY185 pKa = 10.84GRR187 pKa = 11.84IFFVGDD193 pKa = 3.19SRR195 pKa = 11.84TVDD198 pKa = 3.25MFDD201 pKa = 3.55GNAEE205 pKa = 4.06EE206 pKa = 5.11IYY208 pKa = 10.57DD209 pKa = 3.86YY210 pKa = 10.69DD211 pKa = 3.9ASGIRR216 pKa = 11.84VFAKK220 pKa = 10.39DD221 pKa = 3.52GCHH224 pKa = 6.18CAYY227 pKa = 9.25LTDD230 pKa = 3.76VIGRR234 pKa = 11.84YY235 pKa = 7.63GTGEE239 pKa = 4.25FDD241 pKa = 4.75TLVSWLGCNDD251 pKa = 3.4NNDD254 pKa = 3.43AALYY258 pKa = 8.58EE259 pKa = 4.38SVYY262 pKa = 10.79EE263 pKa = 4.08SLISQGKK270 pKa = 6.24TVVICTVGPTADD282 pKa = 3.64EE283 pKa = 4.27SLSGEE288 pKa = 4.03FDD290 pKa = 3.34VANYY294 pKa = 9.79PNEE297 pKa = 3.76KK298 pKa = 9.66MIAFNQSVTGWASAHH313 pKa = 4.95GVKK316 pKa = 10.67VIDD319 pKa = 3.79VYY321 pKa = 11.67SYY323 pKa = 11.0VKK325 pKa = 10.83NNVEE329 pKa = 3.82ISPDD333 pKa = 3.91GIHH336 pKa = 6.38YY337 pKa = 10.14NPKK340 pKa = 8.08PTTAIWNYY348 pKa = 9.84IVSNLL353 pKa = 3.72

Molecular weight: 37.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H7RXR3|A0A1H7RXR3_9FIRM Uncharacterized protein OS=Butyrivibrio sp. ob235 OX=1761780 GN=SAMN04487770_11452 PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 9.98QPHH8 pKa = 6.29KK9 pKa = 8.62LQRR12 pKa = 11.84ARR14 pKa = 11.84VHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.36GGRR28 pKa = 11.84KK29 pKa = 8.93VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.52GRR39 pKa = 11.84KK40 pKa = 9.09KK41 pKa = 10.22ISAA44 pKa = 3.72

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4267

0

4267

1415274

39

3414

331.7

37.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.274 ± 0.054

1.384 ± 0.016

6.477 ± 0.03

7.493 ± 0.041

4.351 ± 0.026

6.918 ± 0.034

1.606 ± 0.017

7.699 ± 0.035

7.227 ± 0.034

8.335 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.057 ± 0.021

4.767 ± 0.028

3.111 ± 0.021

2.641 ± 0.02

4.022 ± 0.029

6.264 ± 0.029

5.443 ± 0.033

6.626 ± 0.028

0.909 ± 0.012

4.398 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski