Hydrogenobacter sp. T-8

Taxonomy: cellular organisms; Bacteria; Aquificae; Aquificae; Aquificales; Aquificaceae; Hydrogenobacter; unclassified Hydrogenobacter

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2006 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6C1BPB5|A0A6C1BPB5_9AQUI Uma2 family endonuclease OS=Hydrogenobacter sp. T-8 OX=1632019 GN=G3M65_01435 PE=4 SV=1
MM1 pKa = 8.3IYY3 pKa = 8.64ITKK6 pKa = 10.37QGDD9 pKa = 3.07RR10 pKa = 11.84WDD12 pKa = 4.12TIAYY16 pKa = 9.02KK17 pKa = 10.73LYY19 pKa = 9.82GDD21 pKa = 4.73PYY23 pKa = 11.16AYY25 pKa = 9.77DD26 pKa = 4.43ALLLYY31 pKa = 10.02NPQYY35 pKa = 11.4AGITVFPAGIQLVVPEE51 pKa = 4.94IEE53 pKa = 4.11YY54 pKa = 11.05EE55 pKa = 4.44DD56 pKa = 3.82INEE59 pKa = 4.44VSPPWQTDD67 pKa = 2.64

Molecular weight: 7.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6C1BVT4|A0A6C1BVT4_9AQUI Uncharacterized protein OS=Hydrogenobacter sp. T-8 OX=1632019 GN=G3M65_09055 PE=4 SV=1
MM1 pKa = 7.24ATQRR5 pKa = 11.84NITRR9 pKa = 11.84ISNLRR14 pKa = 11.84RR15 pKa = 11.84KK16 pKa = 9.7RR17 pKa = 11.84KK18 pKa = 9.73SGFLARR24 pKa = 11.84MEE26 pKa = 4.53TKK28 pKa = 10.22SGRR31 pKa = 11.84AIIKK35 pKa = 9.84RR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84QKK40 pKa = 10.17GRR42 pKa = 11.84KK43 pKa = 9.01RR44 pKa = 11.84LTPP47 pKa = 3.95

Molecular weight: 5.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2006

0

2006

571723

36

1565

285.0

32.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.172 ± 0.045

0.953 ± 0.023

4.526 ± 0.034

8.766 ± 0.078

4.818 ± 0.05

6.987 ± 0.053

1.717 ± 0.021

6.65 ± 0.035

7.361 ± 0.05

11.495 ± 0.086

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.259 ± 0.02

3.068 ± 0.035

4.078 ± 0.043

2.707 ± 0.029

6.018 ± 0.04

5.625 ± 0.041

3.977 ± 0.036

7.677 ± 0.055

1.067 ± 0.02

4.08 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski