Amino acid dipepetide frequency for Hydrogenobacter sp. T-8

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	1.277AlaAla: 1.277 ± 0.062	0.95AlaCys: 0.95 ± 0.042	2.512AlaAsp: 2.512 ± 0.066	4.284AlaGlu: 4.284 ± 0.085	3.446AlaPhe: 3.446 ± 0.104	4.808AlaGly: 4.808 ± 0.094	1.126AlaHis: 1.126 ± 0.045	4.976AlaIle: 4.976 ± 0.109	4.752AlaLys: 4.752 ± 0.096	8.161AlaLeu: 8.161 ± 0.14	1.658AlaMet: 1.658 ± 0.058	1.802AlaAsn: 1.802 ± 0.057	1.894AlaPro: 1.894 ± 0.066	2.209AlaGln: 2.209 ± 0.07	3.343AlaArg: 3.343 ± 0.08	3.556AlaSer: 3.556 ± 0.078	2.304AlaThr: 2.304 ± 0.068	5.207AlaVal: 5.207 ± 0.108	0.525AlaTrp: 0.525 ± 0.03	2.93AlaTyr: 2.93 ± 0.079	0.0AlaXaa: 0.0 ± 0.0
Cys	0.603CysAla: 0.603 ± 0.036	0.122CysCys: 0.122 ± 0.015	0.397CysAsp: 0.397 ± 0.028	0.728CysGlu: 0.728 ± 0.038	0.325CysPhe: 0.325 ± 0.025	1.049CysGly: 1.049 ± 0.047	0.243CysHis: 0.243 ± 0.02	0.554CysIle: 0.554 ± 0.033	0.633CysLys: 0.633 ± 0.036	0.897CysLeu: 0.897 ± 0.04	0.233CysMet: 0.233 ± 0.02	0.301CysAsn: 0.301 ± 0.024	0.672CysPro: 0.672 ± 0.038	0.257CysGln: 0.257 ± 0.02	0.481CysArg: 0.481 ± 0.031	0.539CysSer: 0.539 ± 0.032	0.383CysThr: 0.383 ± 0.024	0.663CysVal: 0.663 ± 0.031	0.087CysTrp: 0.087 ± 0.013	0.359CysTyr: 0.359 ± 0.026	0.0CysXaa: 0.0 ± 0.0
Asp	2.708AspAla: 2.708 ± 0.071	0.453AspCys: 0.453 ± 0.029	1.226AspAsp: 1.226 ± 0.051	2.771AspGlu: 2.771 ± 0.066	2.891AspPhe: 2.891 ± 0.075	2.823AspGly: 2.823 ± 0.072	0.663AspHis: 0.663 ± 0.031	4.208AspIle: 4.208 ± 0.101	3.075AspLys: 3.075 ± 0.087	5.636AspLeu: 5.636 ± 0.112	1.221AspMet: 1.221 ± 0.043	1.126AspAsn: 1.126 ± 0.046	2.41AspPro: 2.41 ± 0.075	0.931AspGln: 0.931 ± 0.046	2.872AspArg: 2.872 ± 0.081	1.774AspSer: 1.774 ± 0.053	2.06AspThr: 2.06 ± 0.06	3.4AspVal: 3.4 ± 0.089	0.68AspTrp: 0.68 ± 0.035	2.335AspTyr: 2.335 ± 0.062	0.0AspXaa: 0.0 ± 0.0
Glu	4.518GluAla: 4.518 ± 0.1	0.631GluCys: 0.631 ± 0.037	5.148GluAsp: 5.148 ± 0.104	10.736GluGlu: 10.736 ± 0.204	3.495GluPhe: 3.495 ± 0.084	7.128GluGly: 7.128 ± 0.14	1.301GluHis: 1.301 ± 0.05	5.237GluIle: 5.237 ± 0.105	7.261GluLys: 7.261 ± 0.144	9.996GluLeu: 9.996 ± 0.174	1.464GluMet: 1.464 ± 0.053	3.001GluAsn: 3.001 ± 0.077	2.582GluPro: 2.582 ± 0.069	1.774GluGln: 1.774 ± 0.064	6.173GluArg: 6.173 ± 0.121	3.367GluSer: 3.367 ± 0.083	2.676GluThr: 2.676 ± 0.075	7.463GluVal: 7.463 ± 0.125	0.805GluTrp: 0.805 ± 0.041	2.904GluTyr: 2.904 ± 0.085	0.0GluXaa: 0.0 ± 0.0
Phe	2.666PheAla: 2.666 ± 0.079	0.497PheCys: 0.497 ± 0.028	2.13PheAsp: 2.13 ± 0.064	3.787PheGlu: 3.787 ± 0.09	2.666PhePhe: 2.666 ± 0.086	3.406PheGly: 3.406 ± 0.077	0.827PheHis: 0.827 ± 0.041	3.203PheIle: 3.203 ± 0.086	2.771PheLys: 2.771 ± 0.081	6.222PheLeu: 6.222 ± 0.133	1.324PheMet: 1.324 ± 0.048	1.41PheAsn: 1.41 ± 0.049	1.851PhePro: 1.851 ± 0.064	1.163PheGln: 1.163 ± 0.05	2.209PheArg: 2.209 ± 0.068	3.474PheSer: 3.474 ± 0.079	2.069PheThr: 2.069 ± 0.059	3.663PheVal: 3.663 ± 0.082	0.574PheTrp: 0.574 ± 0.029	2.276PheTyr: 2.276 ± 0.063	0.0PheXaa: 0.0 ± 0.0
Gly	4.012GlyAla: 4.012 ± 0.085	0.731GlyCys: 0.731 ± 0.041	3.234GlyAsp: 3.234 ± 0.075	5.041GlyGlu: 5.041 ± 0.111	4.112GlyPhe: 4.112 ± 0.088	5.02GlyGly: 5.02 ± 0.118	1.191GlyHis: 1.191 ± 0.051	5.602GlyIle: 5.602 ± 0.116	5.917GlyLys: 5.917 ± 0.115	7.126GlyLeu: 7.126 ± 0.125	1.817GlyMet: 1.817 ± 0.06	2.186GlyAsn: 2.186 ± 0.066	1.457GlyPro: 1.457 ± 0.054	1.835GlyGln: 1.835 ± 0.062	4.042GlyArg: 4.042 ± 0.091	3.671GlySer: 3.671 ± 0.087	2.865GlyThr: 2.865 ± 0.078	5.737GlyVal: 5.737 ± 0.11	0.875GlyTrp: 0.875 ± 0.037	3.4GlyTyr: 3.4 ± 0.082	0.0GlyXaa: 0.0 ± 0.0
His	1.132HisAla: 1.132 ± 0.043	0.222HisCys: 0.222 ± 0.019	0.532HisAsp: 0.532 ± 0.031	1.077HisGlu: 1.077 ± 0.047	0.875HisPhe: 0.875 ± 0.041	1.17HisGly: 1.17 ± 0.044	0.443HisHis: 0.443 ± 0.029	1.481HisIle: 1.481 ± 0.049	0.971HisLys: 0.971 ± 0.039	1.81HisLeu: 1.81 ± 0.059	0.518HisMet: 0.518 ± 0.029	0.505HisAsn: 0.505 ± 0.026	1.083HisPro: 1.083 ± 0.048	0.425HisGln: 0.425 ± 0.031	1.009HisArg: 1.009 ± 0.041	0.906HisSer: 0.906 ± 0.042	0.862HisThr: 0.862 ± 0.047	1.188HisVal: 1.188 ± 0.045	0.192HisTrp: 0.192 ± 0.018	0.77HisTyr: 0.77 ± 0.037	0.0HisXaa: 0.0 ± 0.0
Ile	5.048IleAla: 5.048 ± 0.119	0.518IleCys: 0.518 ± 0.031	3.442IleAsp: 3.442 ± 0.078	5.618IleGlu: 5.618 ± 0.124	2.573IlePhe: 2.573 ± 0.075	4.326IleGly: 4.326 ± 0.08	0.993IleHis: 0.993 ± 0.042	3.621IleIle: 3.621 ± 0.088	5.419IleLys: 5.419 ± 0.094	6.636IleLeu: 6.636 ± 0.122	1.268IleMet: 1.268 ± 0.044	2.585IleAsn: 2.585 ± 0.073	3.147IlePro: 3.147 ± 0.081	1.613IleGln: 1.613 ± 0.05	4.749IleArg: 4.749 ± 0.086	4.032IleSer: 4.032 ± 0.09	3.517IleThr: 3.517 ± 0.078	4.544IleVal: 4.544 ± 0.097	0.512IleTrp: 0.512 ± 0.028	2.344IleTyr: 2.344 ± 0.071	0.0IleXaa: 0.0 ± 0.0
Lys	5.688LysAla: 5.688 ± 0.104	0.388LysCys: 0.388 ± 0.029	4.173LysAsp: 4.173 ± 0.087	8.639LysGlu: 8.639 ± 0.146	2.02LysPhe: 2.02 ± 0.064	5.56LysGly: 5.56 ± 0.111	1.249LysHis: 1.249 ± 0.048	3.822LysIle: 3.822 ± 0.089	5.254LysLys: 5.254 ± 0.121	7.08LysLeu: 7.08 ± 0.13	1.209LysMet: 1.209 ± 0.049	2.527LysAsn: 2.527 ± 0.071	3.343LysPro: 3.343 ± 0.081	1.534LysGln: 1.534 ± 0.056	5.088LysArg: 5.088 ± 0.109	3.671LysSer: 3.671 ± 0.082	3.14LysThr: 3.14 ± 0.074	6.468LysVal: 6.468 ± 0.115	0.532LysTrp: 0.532 ± 0.031	2.225LysTyr: 2.225 ± 0.06	0.0LysXaa: 0.0 ± 0.0
Leu	7.012LeuAla: 7.012 ± 0.13	1.058LeuCys: 1.058 ± 0.043	4.242LeuAsp: 4.242 ± 0.09	9.905LeuGlu: 9.905 ± 0.165	5.531LeuPhe: 5.531 ± 0.138	7.364LeuGly: 7.364 ± 0.139	1.774LeuHis: 1.774 ± 0.063	7.273LeuIle: 7.273 ± 0.118	9.237LeuLys: 9.237 ± 0.145	13.277LeuLeu: 13.277 ± 0.225	2.769LeuMet: 2.769 ± 0.073	3.059LeuAsn: 3.059 ± 0.069	4.719LeuPro: 4.719 ± 0.087	3.337LeuGln: 3.337 ± 0.083	7.6LeuArg: 7.6 ± 0.13	8.711LeuSer: 8.711 ± 0.16	4.703LeuThr: 4.703 ± 0.095	7.357LeuVal: 7.357 ± 0.121	1.401LeuTrp: 1.401 ± 0.056	4.619LeuTyr: 4.619 ± 0.104	0.0LeuXaa: 0.0 ± 0.0
Met	1.754MetAla: 1.754 ± 0.057	0.17MetCys: 0.17 ± 0.014	1.163MetAsp: 1.163 ± 0.049	2.269MetGlu: 2.269 ± 0.067	0.782MetPhe: 0.782 ± 0.043	1.842MetGly: 1.842 ± 0.064	0.43MetHis: 0.43 ± 0.026	0.999MetIle: 0.999 ± 0.044	1.847MetLys: 1.847 ± 0.063	2.503MetLeu: 2.503 ± 0.07	0.313MetMet: 0.313 ± 0.023	0.673MetAsn: 0.673 ± 0.034	1.223MetPro: 1.223 ± 0.044	0.652MetGln: 0.652 ± 0.032	1.336MetArg: 1.336 ± 0.052	1.205MetSer: 1.205 ± 0.049	0.794MetThr: 0.794 ± 0.042	1.854MetVal: 1.854 ± 0.057	0.229MetTrp: 0.229 ± 0.018	0.549MetTyr: 0.549 ± 0.033	0.0MetXaa: 0.0 ± 0.0
Asn	1.959AsnAla: 1.959 ± 0.064	0.38AsnCys: 0.38 ± 0.033	0.775AsnAsp: 0.775 ± 0.038	1.3AsnGlu: 1.3 ± 0.048	1.777AsnPhe: 1.777 ± 0.055	1.478AsnGly: 1.478 ± 0.054	0.539AsnHis: 0.539 ± 0.03	2.485AsnIle: 2.485 ± 0.072	1.725AsnLys: 1.725 ± 0.061	4.637AsnLeu: 4.637 ± 0.089	0.885AsnMet: 0.885 ± 0.035	0.878AsnAsn: 0.878 ± 0.044	2.384AsnPro: 2.384 ± 0.07	0.75AsnGln: 0.75 ± 0.039	1.753AsnArg: 1.753 ± 0.056	1.644AsnSer: 1.644 ± 0.058	1.602AsnThr: 1.602 ± 0.06	1.891AsnVal: 1.891 ± 0.063	0.416AsnTrp: 0.416 ± 0.024	1.419AsnTyr: 1.419 ± 0.055	0.0AsnXaa: 0.0 ± 0.0
Pro	2.564ProAla: 2.564 ± 0.076	0.315ProCys: 0.315 ± 0.026	1.968ProAsp: 1.968 ± 0.062	4.165ProGlu: 4.165 ± 0.088	2.071ProPhe: 2.071 ± 0.068	2.008ProGly: 2.008 ± 0.073	0.876ProHis: 0.876 ± 0.041	2.697ProIle: 2.697 ± 0.062	2.681ProLys: 2.681 ± 0.07	4.088ProLeu: 4.088 ± 0.1	1.041ProMet: 1.041 ± 0.042	1.342ProAsn: 1.342 ± 0.053	1.901ProPro: 1.901 ± 0.083	1.63ProGln: 1.63 ± 0.058	1.49ProArg: 1.49 ± 0.053	2.339ProSer: 2.339 ± 0.07	1.935ProThr: 1.935 ± 0.065	3.255ProVal: 3.255 ± 0.076	0.373ProTrp: 0.373 ± 0.026	2.039ProTyr: 2.039 ± 0.061	0.0ProXaa: 0.0 ± 0.0
Gln	2.074GlnAla: 2.074 ± 0.065	0.212GlnCys: 0.212 ± 0.018	1.268GlnAsp: 1.268 ± 0.044	3.204GlnGlu: 3.204 ± 0.087	0.756GlnPhe: 0.756 ± 0.039	1.669GlnGly: 1.669 ± 0.068	0.427GlnHis: 0.427 ± 0.03	1.728GlnIle: 1.728 ± 0.057	2.517GlnLys: 2.517 ± 0.076	2.085GlnLeu: 2.085 ± 0.055	0.574GlnMet: 0.574 ± 0.032	0.883GlnAsn: 0.883 ± 0.044	0.834GlnPro: 0.834 ± 0.041	0.684GlnGln: 0.684 ± 0.04	2.242GlnArg: 2.242 ± 0.052	1.429GlnSer: 1.429 ± 0.053	1.265GlnThr: 1.265 ± 0.048	2.113GlnVal: 2.113 ± 0.059	0.332GlnTrp: 0.332 ± 0.022	0.771GlnTyr: 0.771 ± 0.036	0.0GlnXaa: 0.0 ± 0.0
Arg	3.871ArgAla: 3.871 ± 0.071	0.455ArgCys: 0.455 ± 0.031	3.127ArgAsp: 3.127 ± 0.078	6.349ArgGlu: 6.349 ± 0.119	2.865ArgPhe: 2.865 ± 0.07	3.817ArgGly: 3.817 ± 0.09	0.997ArgHis: 0.997 ± 0.044	4.091ArgIle: 4.091 ± 0.075	3.955ArgLys: 3.955 ± 0.084	7.584ArgLeu: 7.584 ± 0.126	1.223ArgMet: 1.223 ± 0.046	1.893ArgAsn: 1.893 ± 0.062	1.756ArgPro: 1.756 ± 0.058	1.655ArgGln: 1.655 ± 0.055	3.671ArgArg: 3.671 ± 0.098	2.711ArgSer: 2.711 ± 0.074	1.987ArgThr: 1.987 ± 0.06	5.295ArgVal: 5.295 ± 0.086	0.747ArgTrp: 0.747 ± 0.04	2.132ArgTyr: 2.132 ± 0.063	0.0ArgXaa: 0.0 ± 0.0
Ser	3.736SerAla: 3.736 ± 0.09	0.579SerCys: 0.579 ± 0.032	1.926SerAsp: 1.926 ± 0.062	3.888SerGlu: 3.888 ± 0.083	3.383SerPhe: 3.383 ± 0.086	3.769SerGly: 3.769 ± 0.075	1.018SerHis: 1.018 ± 0.04	3.663SerIle: 3.663 ± 0.074	3.984SerLys: 3.984 ± 0.09	8.058SerLeu: 8.058 ± 0.148	1.49SerMet: 1.49 ± 0.053	1.426SerAsn: 1.426 ± 0.052	2.489SerPro: 2.489 ± 0.066	1.882SerGln: 1.882 ± 0.062	2.683SerArg: 2.683 ± 0.066	3.253SerSer: 3.253 ± 0.095	2.276SerThr: 2.276 ± 0.065	3.654SerVal: 3.654 ± 0.087	0.593SerTrp: 0.593 ± 0.035	2.499SerTyr: 2.499 ± 0.07	0.0SerXaa: 0.0 ± 0.0
Thr	2.97ThrAla: 2.97 ± 0.073	0.422ThrCys: 0.422 ± 0.027	1.802ThrAsp: 1.802 ± 0.061	2.62ThrGlu: 2.62 ± 0.07	2.001ThrPhe: 2.001 ± 0.059	3.294ThrGly: 3.294 ± 0.08	0.798ThrHis: 0.798 ± 0.034	2.47ThrIle: 2.47 ± 0.071	2.384ThrLys: 2.384 ± 0.061	5.022ThrLeu: 5.022 ± 0.103	0.794ThrMet: 0.794 ± 0.042	1.195ThrAsn: 1.195 ± 0.043	2.108ThrPro: 2.108 ± 0.06	1.28ThrGln: 1.28 ± 0.043	1.775ThrArg: 1.775 ± 0.052	2.167ThrSer: 2.167 ± 0.059	1.861ThrThr: 1.861 ± 0.059	2.711ThrVal: 2.711 ± 0.069	0.348ThrTrp: 0.348 ± 0.024	1.749ThrTyr: 1.749 ± 0.06	0.0ThrXaa: 0.0 ± 0.0
Val	4.612ValAla: 4.612 ± 0.11	0.927ValCys: 0.927 ± 0.045	3.918ValAsp: 3.918 ± 0.101	6.877ValGlu: 6.877 ± 0.129	4.261ValPhe: 4.261 ± 0.094	5.658ValGly: 5.658 ± 0.119	1.21ValHis: 1.21 ± 0.045	5.156ValIle: 5.156 ± 0.099	5.623ValLys: 5.623 ± 0.096	8.494ValLeu: 8.494 ± 0.15	1.462ValMet: 1.462 ± 0.044	2.246ValAsn: 2.246 ± 0.07	3.012ValPro: 3.012 ± 0.075	1.854ValGln: 1.854 ± 0.066	4.306ValArg: 4.306 ± 0.094	4.854ValSer: 4.854 ± 0.107	1.167ValThr: 1.167 ± 0.05	6.853ValVal: 6.853 ± 0.139	0.808ValTrp: 0.808 ± 0.036	3.465ValTyr: 3.465 ± 0.083	0.0ValXaa: 0.0 ± 0.0
Trp	0.649TrpAla: 0.649 ± 0.036	0.105TrpCys: 0.105 ± 0.015	0.602TrpAsp: 0.602 ± 0.036	0.999TrpGlu: 0.999 ± 0.042	0.441TrpPhe: 0.441 ± 0.032	0.728TrpGly: 0.728 ± 0.033	0.252TrpHis: 0.252 ± 0.022	0.651TrpIle: 0.651 ± 0.033	0.878TrpLys: 0.878 ± 0.04	1.345TrpLeu: 1.345 ± 0.051	0.25TrpMet: 0.25 ± 0.022	0.345TrpAsn: 0.345 ± 0.024	0.124TrpPro: 0.124 ± 0.014	0.43TrpGln: 0.43 ± 0.031	0.551TrpArg: 0.551 ± 0.026	0.546TrpSer: 0.546 ± 0.033	0.408TrpThr: 0.408 ± 0.028	0.882TrpVal: 0.882 ± 0.045	0.143TrpTrp: 0.143 ± 0.016	0.343TrpTyr: 0.343 ± 0.024	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.865TyrAla: 2.865 ± 0.077	0.394TyrCys: 0.394 ± 0.027	1.679TyrAsp: 1.679 ± 0.056	3.402TyrGlu: 3.402 ± 0.077	1.915TyrPhe: 1.915 ± 0.064	2.958TyrGly: 2.958 ± 0.069	0.813TyrHis: 0.813 ± 0.038	2.587TyrIle: 2.587 ± 0.071	2.727TyrLys: 2.727 ± 0.071	4.291TyrLeu: 4.291 ± 0.102	1.074TyrMet: 1.074 ± 0.047	1.293TyrAsn: 1.293 ± 0.052	1.789TyrPro: 1.789 ± 0.055	1.172TyrGln: 1.172 ± 0.055	2.806TyrArg: 2.806 ± 0.062	2.402TyrSer: 2.402 ± 0.066	1.896TyrThr: 1.896 ± 0.059	2.569TyrVal: 2.569 ± 0.067	0.497TyrTrp: 0.497 ± 0.031	1.674TyrTyr: 1.674 ± 0.062	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2006 proteins (571723 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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