Saccharopolyspora rectivirgula

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Saccharopolyspora

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3222 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A073B2Q2|A0A073B2Q2_9PSEU Uncharacterized protein OS=Saccharopolyspora rectivirgula OX=28042 GN=GU90_01485 PE=4 SV=1
MM1 pKa = 7.75EE2 pKa = 5.45NPLDD6 pKa = 4.03LKK8 pKa = 10.98AVSDD12 pKa = 4.26PCQLLTPEE20 pKa = 4.56QLAQLEE26 pKa = 4.61NPTEE30 pKa = 4.26AEE32 pKa = 3.97PTEE35 pKa = 4.42LEE37 pKa = 4.12WGEE40 pKa = 4.97KK41 pKa = 8.43GCSWYY46 pKa = 10.79GEE48 pKa = 4.16QYY50 pKa = 11.2GLEE53 pKa = 4.17LSPNTISGGFATIRR67 pKa = 11.84QNQKK71 pKa = 10.55SFDD74 pKa = 3.61NYY76 pKa = 10.83QEE78 pKa = 4.29VEE80 pKa = 4.19VAGYY84 pKa = 8.97QAARR88 pKa = 11.84TDD90 pKa = 3.54ATDD93 pKa = 3.74ISCSLWVAVSDD104 pKa = 4.63DD105 pKa = 3.74STLGVQVGKK114 pKa = 10.93YY115 pKa = 9.41DD116 pKa = 4.26AYY118 pKa = 11.15DD119 pKa = 4.69DD120 pKa = 5.27LPQPCEE126 pKa = 3.64LAEE129 pKa = 4.46KK130 pKa = 10.3IFPEE134 pKa = 4.4VVKK137 pKa = 10.81NIPPQDD143 pKa = 3.24

Molecular weight: 15.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A073BBN0|A0A073BBN0_9PSEU RsdA_SigD_bd domain-containing protein OS=Saccharopolyspora rectivirgula OX=28042 GN=GU90_04915 PE=4 SV=1
MM1 pKa = 7.8KK2 pKa = 10.33ARR4 pKa = 11.84LSRR7 pKa = 11.84PSPRR11 pKa = 11.84MVRR14 pKa = 11.84ALALAVLVTQVLISVTGTIVRR35 pKa = 11.84VTGSGLGCPTWPQCFPGSMVPVSHH59 pKa = 7.58AEE61 pKa = 3.65IAPLHH66 pKa = 5.1QAIEE70 pKa = 4.23FGNRR74 pKa = 11.84LLTFVVGIAAIACFLAAWRR93 pKa = 11.84SRR95 pKa = 11.84PRR97 pKa = 11.84RR98 pKa = 11.84PRR100 pKa = 11.84LVRR103 pKa = 11.84LALVMPLGVVAQAIIGGITVLVDD126 pKa = 3.73LQWWSVSLHH135 pKa = 5.66FLASAVLVWLAAILVKK151 pKa = 9.63TASEE155 pKa = 4.27GDD157 pKa = 4.07DD158 pKa = 3.61KK159 pKa = 11.67PMLVITRR166 pKa = 11.84TMQRR170 pKa = 11.84LLVALSAVTFVMLFAGTLVTAAGPHH195 pKa = 6.43AGDD198 pKa = 3.62AATPRR203 pKa = 11.84LDD205 pKa = 3.67VPVEE209 pKa = 4.13TIARR213 pKa = 11.84VHH215 pKa = 6.66SMSTMAYY222 pKa = 8.87VLLLAVFGVWLRR234 pKa = 11.84SARR237 pKa = 11.84PTKK240 pKa = 10.65ALLKK244 pKa = 10.23AYY246 pKa = 9.99SAACAVVLLQGAIGYY261 pKa = 6.2TQYY264 pKa = 11.95ALGVPEE270 pKa = 4.66PLVVAHH276 pKa = 6.19VLGAMLVVVATSWLWCEE293 pKa = 3.65SRR295 pKa = 11.84YY296 pKa = 10.13RR297 pKa = 11.84GPLPQQKK304 pKa = 9.03QAEE307 pKa = 4.34PAASS311 pKa = 3.2

Molecular weight: 33.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3222

0

3222

1004369

37

1646

311.7

33.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.221 ± 0.062

0.847 ± 0.013

5.632 ± 0.034

6.696 ± 0.037

2.865 ± 0.025

8.788 ± 0.042

2.222 ± 0.02

3.587 ± 0.029

2.32 ± 0.025

10.433 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.776 ± 0.017

2.103 ± 0.019

5.861 ± 0.039

3.609 ± 0.028

7.835 ± 0.045

5.304 ± 0.028

5.478 ± 0.029

8.957 ± 0.043

1.462 ± 0.018

2.003 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski