Chryseobacterium sp. NBC 122
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2837 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P6ZHS0|A0A4P6ZHS0_9FLAO Uncharacterized protein OS=Chryseobacterium sp. NBC 122 OX=2547600 GN=NBC122_02322 PE=4 SV=1
MM1 pKa = 7.56 QDD3 pKa = 2.66 ITLKK7 pKa = 9.36 ITDD10 pKa = 3.89 RR11 pKa = 11.84 NGDD14 pKa = 3.46 LHH16 pKa = 6.9 EE17 pKa = 4.64 VVAPTDD23 pKa = 3.19 MSMNLMEE30 pKa = 4.84 VIRR33 pKa = 11.84 SYY35 pKa = 11.43 EE36 pKa = 3.86 LADD39 pKa = 3.65 EE40 pKa = 4.45 GTIGVCGGMAMCASCQVYY58 pKa = 9.06 VVEE61 pKa = 5.61 GSDD64 pKa = 3.4 KK65 pKa = 9.34 LTEE68 pKa = 4.01 MGAEE72 pKa = 4.0 EE73 pKa = 4.77 DD74 pKa = 4.01 AMLSEE79 pKa = 4.71 AFHH82 pKa = 6.02 VQDD85 pKa = 4.44 NSRR88 pKa = 11.84 LGCQIHH94 pKa = 6.05 ITSEE98 pKa = 3.72 IDD100 pKa = 3.34 GLEE103 pKa = 4.21 VAIAPYY109 pKa = 9.81 PP110 pKa = 3.58
Molecular weight: 11.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.814
IPC2_protein 4.05
IPC_protein 3.973
Toseland 3.795
ProMoST 4.113
Dawson 3.948
Bjellqvist 4.101
Wikipedia 3.846
Rodwell 3.808
Grimsley 3.706
Solomon 3.935
Lehninger 3.884
Nozaki 4.062
DTASelect 4.228
Thurlkill 3.834
EMBOSS 3.859
Sillero 4.101
Patrickios 0.947
IPC_peptide 3.935
IPC2_peptide 4.075
IPC2.peptide.svr19 3.967
Protein with the highest isoelectric point:
>tr|A0A4P6ZF48|A0A4P6ZF48_9FLAO Arsenical-resistance protein Acr3 OS=Chryseobacterium sp. NBC 122 OX=2547600 GN=acr3 PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPSEE10 pKa = 3.58 RR11 pKa = 11.84 KK12 pKa = 9.37 KK13 pKa = 10.2 RR14 pKa = 11.84 NKK16 pKa = 9.83 HH17 pKa = 3.9 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MSTPNGRR29 pKa = 11.84 RR30 pKa = 11.84 ILAARR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 9.99 GRR40 pKa = 11.84 KK41 pKa = 8.8 SLTVSAQRR49 pKa = 11.84 AKK51 pKa = 10.68 RR52 pKa = 3.52
Molecular weight: 6.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.444
IPC2_protein 10.965
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.427
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.149
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.085
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2837
0
2837
898352
29
2403
316.7
35.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.379 ± 0.047
0.728 ± 0.014
5.306 ± 0.032
6.742 ± 0.051
5.534 ± 0.037
6.342 ± 0.041
1.759 ± 0.023
7.885 ± 0.049
8.274 ± 0.043
9.207 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.448 ± 0.024
6.102 ± 0.049
3.471 ± 0.023
3.593 ± 0.025
3.41 ± 0.031
6.398 ± 0.042
5.446 ± 0.04
6.007 ± 0.035
0.999 ± 0.017
3.97 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here