Dysgonomonas mossii DSM 22836
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3424 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F8WWB1|F8WWB1_9BACT PASTA domain-containing protein OS=Dysgonomonas mossii DSM 22836 OX=742767 GN=HMPREF9456_00080 PE=4 SV=1
MM1 pKa = 7.61 SSDD4 pKa = 4.01 KK5 pKa = 11.27 DD6 pKa = 3.94 LIQYY10 pKa = 10.67 DD11 pKa = 3.41 EE12 pKa = 5.2 DD13 pKa = 4.37 DD14 pKa = 3.62 AVKK17 pKa = 10.63 YY18 pKa = 8.68 IQNYY22 pKa = 9.11 LPQEE26 pKa = 4.24 VKK28 pKa = 10.64 GKK30 pKa = 10.44 YY31 pKa = 10.08 SSDD34 pKa = 3.58 EE35 pKa = 3.55 INYY38 pKa = 9.96 IIDD41 pKa = 3.28 IVYY44 pKa = 10.1 EE45 pKa = 4.31 FYY47 pKa = 10.9 EE48 pKa = 4.21 EE49 pKa = 3.84 KK50 pKa = 10.97 GYY52 pKa = 9.77 MDD54 pKa = 4.45 EE55 pKa = 5.56 DD56 pKa = 3.93 ADD58 pKa = 4.02 EE59 pKa = 4.17 NVIVDD64 pKa = 3.43 IDD66 pKa = 3.61 EE67 pKa = 4.87 DD68 pKa = 4.62 EE69 pKa = 4.21 IVSYY73 pKa = 9.76 VLKK76 pKa = 9.48 NTKK79 pKa = 9.32 KK80 pKa = 10.71 DD81 pKa = 3.42 KK82 pKa = 10.87 INNFSAEE89 pKa = 4.54 DD90 pKa = 3.32 ISFIIQGEE98 pKa = 3.98 IAYY101 pKa = 9.92 CEE103 pKa = 3.9 SLGIFEE109 pKa = 4.67
Molecular weight: 12.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.712
IPC2_protein 3.783
IPC_protein 3.757
Toseland 3.554
ProMoST 3.897
Dawson 3.745
Bjellqvist 3.935
Wikipedia 3.668
Rodwell 3.592
Grimsley 3.465
Solomon 3.732
Lehninger 3.694
Nozaki 3.859
DTASelect 4.062
Thurlkill 3.605
EMBOSS 3.681
Sillero 3.884
Patrickios 0.985
IPC_peptide 3.732
IPC2_peptide 3.859
IPC2.peptide.svr19 3.798
Protein with the highest isoelectric point:
>tr|F8X552|F8X552_9BACT Uncharacterized protein OS=Dysgonomonas mossii DSM 22836 OX=742767 GN=HMPREF9456_03361 PE=4 SV=1
MM1 pKa = 8.0 RR2 pKa = 11.84 VRR4 pKa = 11.84 QRR6 pKa = 11.84 ASTTKK11 pKa = 9.91 CNKK14 pKa = 9.68 MKK16 pKa = 10.79 DD17 pKa = 3.58 KK18 pKa = 10.34 LLKK21 pKa = 10.23 RR22 pKa = 11.84 IRR24 pKa = 11.84 EE25 pKa = 3.75 NWFIGISEE33 pKa = 4.58 SEE35 pKa = 4.32 TYY37 pKa = 10.63 ILVQKK42 pKa = 10.5 KK43 pKa = 10.04 GLDD46 pKa = 3.22 AGEE49 pKa = 4.34 FCVSRR54 pKa = 11.84 EE55 pKa = 3.96 FYY57 pKa = 10.54 IHH59 pKa = 6.84 ILKK62 pKa = 10.69 LYY64 pKa = 8.47 GLCSDD69 pKa = 4.04 ISWFIAYY76 pKa = 9.63 RR77 pKa = 11.84 HH78 pKa = 5.53 FSKK81 pKa = 10.51 LRR83 pKa = 11.84 KK84 pKa = 9.19 RR85 pKa = 11.84 KK86 pKa = 7.28 YY87 pKa = 8.73 HH88 pKa = 6.84 RR89 pKa = 11.84 NRR91 pKa = 11.84 QRR93 pKa = 11.84 MRR95 pKa = 11.84 QSLLNKK101 pKa = 9.94 ISRR104 pKa = 3.83
Molecular weight: 12.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.334
IPC2_protein 9.867
IPC_protein 10.555
Toseland 10.818
ProMoST 10.628
Dawson 10.906
Bjellqvist 10.599
Wikipedia 11.096
Rodwell 11.242
Grimsley 10.95
Solomon 11.008
Lehninger 10.979
Nozaki 10.804
DTASelect 10.584
Thurlkill 10.818
EMBOSS 11.213
Sillero 10.833
Patrickios 10.965
IPC_peptide 11.008
IPC2_peptide 9.575
IPC2.peptide.svr19 8.516
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3424
0
3424
1182442
29
2901
345.3
39.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.36 ± 0.039
0.945 ± 0.012
5.753 ± 0.031
6.336 ± 0.044
4.784 ± 0.029
6.465 ± 0.041
1.659 ± 0.018
8.106 ± 0.04
7.639 ± 0.038
8.973 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.483 ± 0.017
5.727 ± 0.036
3.451 ± 0.019
3.339 ± 0.024
3.91 ± 0.023
6.674 ± 0.033
5.493 ± 0.031
6.138 ± 0.037
1.161 ± 0.017
4.605 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here