Clostridium celatum DSM 1785

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; Clostridium celatum

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3453 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L1QMR2|L1QMR2_9CLOT Uncharacterized protein OS=Clostridium celatum DSM 1785 OX=545697 GN=HMPREF0216_00333 PE=4 SV=1
MM1 pKa = 7.24QKK3 pKa = 10.35LKK5 pKa = 10.95KK6 pKa = 10.16FISLTVSLAFLLSFIGNQGITFAQDD31 pKa = 2.94NPDD34 pKa = 3.55AEE36 pKa = 4.36NSIEE40 pKa = 3.93AQEE43 pKa = 4.01AMKK46 pKa = 10.86EE47 pKa = 4.07EE48 pKa = 4.24TFLDD52 pKa = 3.82EE53 pKa = 4.8SLNEE57 pKa = 3.89ATILEE62 pKa = 4.36EE63 pKa = 4.3TEE65 pKa = 4.11EE66 pKa = 4.01EE67 pKa = 4.68LIDD70 pKa = 4.97EE71 pKa = 4.35ITPVIDD77 pKa = 3.32EE78 pKa = 4.5TTSDD82 pKa = 3.51EE83 pKa = 4.97AEE85 pKa = 3.79ITTDD89 pKa = 3.03INEE92 pKa = 4.39STTSNEE98 pKa = 4.27TINNEE103 pKa = 4.07DD104 pKa = 3.86NLDD107 pKa = 3.62EE108 pKa = 4.95VIIEE112 pKa = 4.18DD113 pKa = 3.35VSEE116 pKa = 4.22IEE118 pKa = 4.73EE119 pKa = 4.33FPKK122 pKa = 10.3DD123 pKa = 3.34TSTDD127 pKa = 3.15NTNEE131 pKa = 3.59IEE133 pKa = 4.28AEE135 pKa = 4.08EE136 pKa = 4.29NPILTNGTSEE146 pKa = 3.87EE147 pKa = 4.06VSEE150 pKa = 4.34NEE152 pKa = 3.77NSKK155 pKa = 8.68EE156 pKa = 4.02TTLEE160 pKa = 3.95EE161 pKa = 4.17KK162 pKa = 10.79VEE164 pKa = 4.14EE165 pKa = 4.12VQIPSSIDD173 pKa = 3.74DD174 pKa = 3.56IEE176 pKa = 4.61VYY178 pKa = 10.67SIEE181 pKa = 4.33TNGTSFDD188 pKa = 3.71FSNYY192 pKa = 9.25TILPSITLNVAEE204 pKa = 5.32EE205 pKa = 4.03FNQYY209 pKa = 9.99ISTQITLVNINDD221 pKa = 3.78TSKK224 pKa = 8.92TVYY227 pKa = 8.13YY228 pKa = 10.02TSYY231 pKa = 10.79GNNISTFVPEE241 pKa = 4.28TTSVSGTYY249 pKa = 9.46EE250 pKa = 3.33ISKK253 pKa = 10.31IEE255 pKa = 4.48IILDD259 pKa = 3.48TTYY262 pKa = 7.9TTLAYY267 pKa = 9.72INPKK271 pKa = 9.99YY272 pKa = 10.72KK273 pKa = 10.71DD274 pKa = 3.13ITTSSSNRR282 pKa = 11.84TEE284 pKa = 4.33YY285 pKa = 11.04KK286 pKa = 10.48DD287 pKa = 3.47LAPNFQLEE295 pKa = 4.56IINYY299 pKa = 9.15EE300 pKa = 3.71PFKK303 pKa = 11.19YY304 pKa = 10.36SFEE307 pKa = 4.25NFSVPHH313 pKa = 6.02IVSNGNEE320 pKa = 3.72FTATVTIKK328 pKa = 10.68SNRR331 pKa = 11.84EE332 pKa = 3.51ITGLTIWYY340 pKa = 10.03QSDD343 pKa = 3.23NDD345 pKa = 4.99SIVLYY350 pKa = 10.29DD351 pKa = 3.56NSVIKK356 pKa = 10.99DD357 pKa = 3.39GDD359 pKa = 3.71NYY361 pKa = 10.19TISPIPYY368 pKa = 9.41VYY370 pKa = 10.61GQGYY374 pKa = 7.44TPFEE378 pKa = 4.08YY379 pKa = 10.38LKK381 pKa = 10.66SGTYY385 pKa = 9.9SVEE388 pKa = 4.09SMIIEE393 pKa = 4.19YY394 pKa = 10.15KK395 pKa = 10.34YY396 pKa = 10.9DD397 pKa = 3.45SSDD400 pKa = 3.12IFATSYY406 pKa = 11.31LSISNITSDD415 pKa = 5.7DD416 pKa = 3.48EE417 pKa = 4.57FEE419 pKa = 5.19NDD421 pKa = 3.57DD422 pKa = 4.51SYY424 pKa = 12.16DD425 pKa = 3.77FSPLNIICNNPNEE438 pKa = 4.78DD439 pKa = 3.48LTPPVINSITMEE451 pKa = 4.04KK452 pKa = 10.71NIINLNEE459 pKa = 3.69VDD461 pKa = 3.57TSDD464 pKa = 4.26GIKK467 pKa = 10.24INISADD473 pKa = 3.68VIDD476 pKa = 5.03DD477 pKa = 4.04LSGFSNMDD485 pKa = 2.32TWMFLVWNSPNGTSPIYY502 pKa = 10.67SEE504 pKa = 5.71LYY506 pKa = 10.84YY507 pKa = 10.78NDD509 pKa = 3.64SSNMFEE515 pKa = 4.88GYY517 pKa = 10.64VSIDD521 pKa = 3.26KK522 pKa = 10.8DD523 pKa = 3.37HH524 pKa = 7.08LYY526 pKa = 10.94EE527 pKa = 5.27GIYY530 pKa = 9.6TLEE533 pKa = 4.38FLSVWDD539 pKa = 4.01RR540 pKa = 11.84ANNLLSYY547 pKa = 10.73DD548 pKa = 4.06RR549 pKa = 11.84EE550 pKa = 4.18TDD552 pKa = 3.96SEE554 pKa = 4.3FLSNYY559 pKa = 9.91DD560 pKa = 2.93ITITRR565 pKa = 11.84DD566 pKa = 3.14SLNNNGSDD574 pKa = 3.69NNNNNNTSNKK584 pKa = 9.65PNDD587 pKa = 3.52SSNIGSNLSGNNSTITITPTNNNTSSQVSSNSSTLNKK624 pKa = 7.53TTLPYY629 pKa = 9.45TGSPISSIFIIIFGSLISISGFYY652 pKa = 9.87MILRR656 pKa = 11.84KK657 pKa = 9.62KK658 pKa = 9.09VNKK661 pKa = 9.65VV662 pKa = 2.78

Molecular weight: 74.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L1Q2P5|L1Q2P5_9CLOT LPXTG-motif protein cell wall anchor domain protein OS=Clostridium celatum DSM 1785 OX=545697 GN=HMPREF0216_03292 PE=4 SV=1
MM1 pKa = 7.99DD2 pKa = 6.68AIRR5 pKa = 11.84KK6 pKa = 8.72QEE8 pKa = 4.28LIKK11 pKa = 10.74QYY13 pKa = 10.62GRR15 pKa = 11.84HH16 pKa = 5.76EE17 pKa = 4.94GDD19 pKa = 2.91TGSPEE24 pKa = 3.87VQIALLTEE32 pKa = 5.33RR33 pKa = 11.84INSLTGHH40 pKa = 6.4LRR42 pKa = 11.84VHH44 pKa = 6.56KK45 pKa = 9.8KK46 pKa = 9.64DD47 pKa = 3.08HH48 pKa = 6.05HH49 pKa = 5.92SRR51 pKa = 11.84RR52 pKa = 11.84GLLMMVGQRR61 pKa = 11.84RR62 pKa = 11.84GLLNYY67 pKa = 10.07LAAQDD72 pKa = 3.52IEE74 pKa = 4.57RR75 pKa = 11.84YY76 pKa = 9.02RR77 pKa = 11.84AIIAQLGLRR86 pKa = 11.84RR87 pKa = 3.9

Molecular weight: 10.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3453

0

3453

1020863

30

2284

295.6

33.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.613 ± 0.048

1.158 ± 0.016

5.591 ± 0.037

7.857 ± 0.049

4.309 ± 0.031

6.393 ± 0.042

1.242 ± 0.014

10.1 ± 0.065

8.738 ± 0.048

8.875 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.514 ± 0.024

6.876 ± 0.049

2.603 ± 0.02

2.093 ± 0.021

3.243 ± 0.029

6.267 ± 0.033

5.018 ± 0.037

6.533 ± 0.042

0.699 ± 0.013

4.279 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski