Chitinophaga jiangningensis
Average proteome isoelectric point is 6.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5782 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M7IQ35|A0A1M7IQ35_9BACT KDO2-lipid IV(A) lauroyltransferase OS=Chitinophaga jiangningensis OX=1419482 GN=SAMN05444266_10854 PE=4 SV=1
MM1 pKa = 7.44 KK2 pKa = 10.59 NSARR6 pKa = 11.84 LVALQPTPQQLTDD19 pKa = 3.54 YY20 pKa = 9.98 ANALIVVNSYY30 pKa = 9.36 TYY32 pKa = 10.94 AVEE35 pKa = 4.08 NQQLPTLNAPPTNYY49 pKa = 10.58 ADD51 pKa = 5.19 FISAFNPVKK60 pKa = 10.59 SSAINWSEE68 pKa = 4.14 TIFPSITTLPAHH80 pKa = 7.5 IINYY84 pKa = 9.69 AGSLFNLEE92 pKa = 4.07 EE93 pKa = 4.18 TLVTGYY99 pKa = 11.33 LNILINDD106 pKa = 4.51 PGNVAARR113 pKa = 11.84 NGLVATLTNMQQTIQAEE130 pKa = 4.58 LNFVTDD136 pKa = 3.3 IQSNLTAFTTDD147 pKa = 2.98 TQTAGQTLQQLAQQALSMAGADD169 pKa = 3.22 KK170 pKa = 8.69 TTIQSLNSQIIRR182 pKa = 11.84 LNNDD186 pKa = 2.68 VANAQQLLTVSEE198 pKa = 4.38 IGLAASIFVGLIGLVVCMVPGAQGIGVGIIIFGIAGLGASIAGTVVFSKK247 pKa = 10.49 EE248 pKa = 4.02 VQDD251 pKa = 4.12 LQNSINSLQGQVSGINQDD269 pKa = 3.59 IIQLQGVSGQINALQAASVQAVQALSTISGMWSQLDD305 pKa = 3.6 AAIGAVAAEE314 pKa = 4.51 LDD316 pKa = 4.21 DD317 pKa = 4.09 VNNDD321 pKa = 2.66 ITSEE325 pKa = 4.06 QFKK328 pKa = 10.47 QALSDD333 pKa = 3.73 FQAAEE338 pKa = 4.22 ANWTDD343 pKa = 3.63 VITFATSLANINYY356 pKa = 9.04 SWQDD360 pKa = 3.13 SSGTWHH366 pKa = 7.38 HH367 pKa = 6.8 YY368 pKa = 7.29 GTQNPAADD376 pKa = 4.03 NGQVTAIPSSQAVAA390 pKa = 3.54
Molecular weight: 41.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.752
IPC2_protein 3.91
IPC_protein 3.897
Toseland 3.681
ProMoST 4.062
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.821
Rodwell 3.719
Grimsley 3.592
Solomon 3.872
Lehninger 3.834
Nozaki 3.999
DTASelect 4.24
Thurlkill 3.732
EMBOSS 3.834
Sillero 4.012
Patrickios 1.914
IPC_peptide 3.872
IPC2_peptide 3.986
IPC2.peptide.svr19 3.901
Protein with the highest isoelectric point:
>tr|A0A1M6WBX4|A0A1M6WBX4_9BACT Muramoyltetrapeptide carboxypeptidase OS=Chitinophaga jiangningensis OX=1419482 GN=SAMN05444266_101565 PE=3 SV=1
MM1 pKa = 6.88 MQDD4 pKa = 3.46 EE5 pKa = 4.55 QNKK8 pKa = 8.02 RR9 pKa = 11.84 TDD11 pKa = 2.87 NRR13 pKa = 11.84 YY14 pKa = 9.35 RR15 pKa = 11.84 PIGEE19 pKa = 4.08 LVRR22 pKa = 11.84 GLFFILFGLFAIFARR37 pKa = 11.84 QLGLGEE43 pKa = 4.63 FRR45 pKa = 11.84 LSPQWMTILGVVLCIYY61 pKa = 10.99 GLFRR65 pKa = 11.84 VINGARR71 pKa = 11.84 KK72 pKa = 9.62 LFF74 pKa = 3.93
Molecular weight: 8.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.338
IPC2_protein 9.619
IPC_protein 10.57
Toseland 10.467
ProMoST 10.277
Dawson 10.628
Bjellqvist 10.394
Wikipedia 10.877
Rodwell 10.701
Grimsley 10.701
Solomon 10.76
Lehninger 10.716
Nozaki 10.482
DTASelect 10.379
Thurlkill 10.511
EMBOSS 10.891
Sillero 10.555
Patrickios 10.54
IPC_peptide 10.76
IPC2_peptide 9.56
IPC2.peptide.svr19 8.44
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5782
0
5782
2166146
29
4281
374.6
41.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.439 ± 0.036
0.814 ± 0.012
5.191 ± 0.023
5.204 ± 0.04
4.486 ± 0.025
7.079 ± 0.035
1.949 ± 0.018
6.456 ± 0.025
6.012 ± 0.036
9.385 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.471 ± 0.019
5.365 ± 0.037
4.266 ± 0.019
4.14 ± 0.018
4.311 ± 0.022
5.994 ± 0.028
6.309 ± 0.054
6.574 ± 0.026
1.325 ± 0.013
4.23 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here