Frateuria terrea
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3427 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H6UG85|A0A1H6UG85_9GAMM Heme exporter protein B OS=Frateuria terrea OX=529704 GN=SAMN04487997_1880 PE=3 SV=1
MM1 pKa = 7.32 NKK3 pKa = 9.32 IFKK6 pKa = 9.98 SALAAAVLIALSSLGGTAYY25 pKa = 10.52 AATATSDD32 pKa = 3.85 LPVSASVTATCTIDD46 pKa = 3.51 ASGGLAFGAYY56 pKa = 10.25 DD57 pKa = 3.42 PVVTNASTDD66 pKa = 3.46 LTGQGTIDD74 pKa = 3.51 TTCTNGASVTVTLGQGANADD94 pKa = 4.06 TGSSDD99 pKa = 3.26 AAPVRR104 pKa = 11.84 RR105 pKa = 11.84 MLSGASDD112 pKa = 3.61 YY113 pKa = 11.36 LSYY116 pKa = 11.04 QLYY119 pKa = 9.93 SDD121 pKa = 4.44 SGRR124 pKa = 11.84 STVWGNTAGTGAPVTGTGTVVSTTVYY150 pKa = 11.12 GLITAGQNVPSGSYY164 pKa = 10.5 SDD166 pKa = 3.8 TVVATVTFF174 pKa = 4.26
Molecular weight: 17.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.858
IPC2_protein 3.935
IPC_protein 3.884
Toseland 3.643
ProMoST 4.113
Dawson 3.923
Bjellqvist 4.088
Wikipedia 3.961
Rodwell 3.719
Grimsley 3.567
Solomon 3.91
Lehninger 3.859
Nozaki 4.062
DTASelect 4.406
Thurlkill 3.745
EMBOSS 3.948
Sillero 4.024
Patrickios 0.299
IPC_peptide 3.897
IPC2_peptide 3.986
IPC2.peptide.svr19 3.909
Protein with the highest isoelectric point:
>tr|A0A1H6SU39|A0A1H6SU39_9GAMM Glucose-6-phosphate 1-dehydrogenase OS=Frateuria terrea OX=529704 GN=zwf PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 10.06 LKK11 pKa = 10.38 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.89 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATADD26 pKa = 3.35 GRR28 pKa = 11.84 KK29 pKa = 9.36 VLNARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.75 GRR39 pKa = 11.84 KK40 pKa = 8.92 RR41 pKa = 11.84 LIPP44 pKa = 4.02
Molecular weight: 5.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.403
IPC2_protein 10.862
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.067
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3427
0
3427
1139055
31
3079
332.4
36.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.114 ± 0.065
0.868 ± 0.015
5.694 ± 0.036
5.177 ± 0.048
3.323 ± 0.022
8.639 ± 0.051
2.543 ± 0.023
3.892 ± 0.03
2.696 ± 0.043
11.098 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.197 ± 0.019
2.39 ± 0.033
5.531 ± 0.035
3.792 ± 0.028
7.74 ± 0.052
5.023 ± 0.037
4.865 ± 0.044
7.424 ± 0.035
1.576 ± 0.021
2.417 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here