Facklamia tabacinasalis
Average proteome isoelectric point is 5.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2319 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5R9DV39|A0A5R9DV39_9LACT Alanine racemase OS=Facklamia tabacinasalis OX=87458 GN=alr PE=3 SV=1
MM1 pKa = 7.57 KK2 pKa = 10.44 KK3 pKa = 9.93 HH4 pKa = 6.21 IKK6 pKa = 10.29 LLLFSLSLFLLFVPQTRR23 pKa = 11.84 PKK25 pKa = 11.03 AEE27 pKa = 3.74 VSLDD31 pKa = 3.55 EE32 pKa = 4.53 FVGEE36 pKa = 4.34 TVTIGTVGEE45 pKa = 4.06 EE46 pKa = 4.58 LADD49 pKa = 2.98 IWEE52 pKa = 4.57 FVADD56 pKa = 4.24 KK57 pKa = 10.65 AAEE60 pKa = 4.26 DD61 pKa = 4.74 GIEE64 pKa = 3.92 LDD66 pKa = 3.47 IVLFTDD72 pKa = 3.63 YY73 pKa = 8.46 NTPNISLVDD82 pKa = 3.9 GSLDD86 pKa = 3.32 MNAYY90 pKa = 9.27 QHH92 pKa = 6.68 TDD94 pKa = 4.01 FLNDD98 pKa = 3.12 WNDD101 pKa = 3.42 ANEE104 pKa = 4.21 TNLVSIGNTFATPLRR119 pKa = 11.84 FFSEE123 pKa = 4.1 KK124 pKa = 10.39 HH125 pKa = 5.52 NSIDD129 pKa = 4.41 DD130 pKa = 4.04 LPEE133 pKa = 3.85 GAIIAIPNDD142 pKa = 3.7 LASSSYY148 pKa = 11.2 ALQILDD154 pKa = 3.65 YY155 pKa = 11.09 ADD157 pKa = 4.04 LVKK160 pKa = 10.6 LDD162 pKa = 4.17 EE163 pKa = 5.72 SIDD166 pKa = 3.75 ALPTVNDD173 pKa = 3.15 IVEE176 pKa = 4.27 NPNNYY181 pKa = 10.06 EE182 pKa = 4.07 IIEE185 pKa = 4.27 LEE187 pKa = 4.0 AAQIPSAMADD197 pKa = 2.72 VDD199 pKa = 3.66 MGVIHH204 pKa = 6.26 QVYY207 pKa = 10.25 LEE209 pKa = 4.23 STDD212 pKa = 4.84 FEE214 pKa = 5.15 PNDD217 pKa = 4.13 AIYY220 pKa = 10.39 SYY222 pKa = 11.14 GHH224 pKa = 5.05 NTEE227 pKa = 3.94 TFNYY231 pKa = 9.7 NRR233 pKa = 11.84 LNTITVRR240 pKa = 11.84 EE241 pKa = 4.03 EE242 pKa = 3.95 DD243 pKa = 3.31 QGNPLYY249 pKa = 10.64 QYY251 pKa = 10.74 IVDD254 pKa = 5.32 LYY256 pKa = 11.2 QSDD259 pKa = 4.58 DD260 pKa = 3.42 VADD263 pKa = 4.25 YY264 pKa = 11.0 IEE266 pKa = 5.03 EE267 pKa = 4.1 ISNGGHH273 pKa = 5.05 IPAWILLEE281 pKa = 4.23 EE282 pKa = 4.54 YY283 pKa = 9.16 EE284 pKa = 4.75 ASLEE288 pKa = 4.08 NEE290 pKa = 4.3 RR291 pKa = 11.84 NN292 pKa = 3.44
Molecular weight: 32.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.701
IPC2_protein 3.783
IPC_protein 3.783
Toseland 3.579
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.668
Rodwell 3.617
Grimsley 3.49
Solomon 3.757
Lehninger 3.719
Nozaki 3.872
DTASelect 4.075
Thurlkill 3.617
EMBOSS 3.681
Sillero 3.91
Patrickios 1.252
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.8
Protein with the highest isoelectric point:
>tr|A0A5R9EN85|A0A5R9EN85_9LACT 6-phospho-beta-glucosidase OS=Facklamia tabacinasalis OX=87458 GN=FEZ33_02775 PE=3 SV=1
MM1 pKa = 7.62 TKK3 pKa = 9.13 RR4 pKa = 11.84 TYY6 pKa = 9.74 QPKK9 pKa = 8.54 KK10 pKa = 7.82 RR11 pKa = 11.84 KK12 pKa = 9.02 RR13 pKa = 11.84 SRR15 pKa = 11.84 VHH17 pKa = 6.41 GFRR20 pKa = 11.84 KK21 pKa = 10.02 RR22 pKa = 11.84 MSTKK26 pKa = 10.14 NGRR29 pKa = 11.84 NVLKK33 pKa = 10.48 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 LKK38 pKa = 10.51 GRR40 pKa = 11.84 KK41 pKa = 9.06 KK42 pKa = 10.22 ISAA45 pKa = 3.72
Molecular weight: 5.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.436
IPC2_protein 11.14
IPC_protein 12.501
Toseland 12.676
ProMoST 13.159
Dawson 12.676
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.486
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.193
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.032
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2319
0
2319
704389
29
1668
303.7
34.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.814 ± 0.051
0.412 ± 0.011
6.004 ± 0.046
7.987 ± 0.065
4.377 ± 0.037
6.358 ± 0.044
1.911 ± 0.026
8.087 ± 0.057
5.705 ± 0.051
9.479 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.619 ± 0.023
5.261 ± 0.037
3.346 ± 0.025
4.043 ± 0.038
3.893 ± 0.04
6.253 ± 0.039
5.729 ± 0.036
6.947 ± 0.038
0.875 ± 0.017
3.899 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here