Aquimarina sp. RZ0
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5315 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5B1BF71|A0A5B1BF71_9FLAO Uncharacterized protein OS=Aquimarina sp. RZ0 OX=2607730 GN=F0000_06420 PE=4 SV=1
MM1 pKa = 7.64 KK2 pKa = 10.48 KK3 pKa = 10.2 LLYY6 pKa = 10.44 YY7 pKa = 11.11 SMIIIAAIGCEE18 pKa = 4.02 DD19 pKa = 4.29 VIDD22 pKa = 3.88 VDD24 pKa = 4.93 VPNGDD29 pKa = 3.37 PRR31 pKa = 11.84 LVIDD35 pKa = 4.91 ASFEE39 pKa = 3.95 FSKK42 pKa = 11.49 DD43 pKa = 3.0 EE44 pKa = 5.35 DD45 pKa = 3.86 GLLDD49 pKa = 4.09 FSQDD53 pKa = 3.68 EE54 pKa = 4.16 IRR56 pKa = 11.84 LTLSAPFFDD65 pKa = 4.7 EE66 pKa = 5.26 NIPTVSNATIFITNLSRR83 pKa = 11.84 DD84 pKa = 3.32 LVLTFRR90 pKa = 11.84 EE91 pKa = 4.74 SIFEE95 pKa = 4.12 PGIYY99 pKa = 9.71 RR100 pKa = 11.84 PSNGSYY106 pKa = 10.5 SFNFGDD112 pKa = 4.03 EE113 pKa = 4.39 YY114 pKa = 10.78 EE115 pKa = 4.32 LTVIYY120 pKa = 10.45 DD121 pKa = 3.27 NQTYY125 pKa = 10.62 KK126 pKa = 11.1 GITQIAPSSPIDD138 pKa = 3.91 AVIQGDD144 pKa = 3.97 GTLFDD149 pKa = 5.88 DD150 pKa = 5.54 DD151 pKa = 5.58 DD152 pKa = 5.44 IEE154 pKa = 5.73 IEE156 pKa = 5.56 LFFTDD161 pKa = 5.71 DD162 pKa = 3.54 GDD164 pKa = 3.78 INNYY168 pKa = 9.64 YY169 pKa = 10.84 LFDD172 pKa = 4.34 FDD174 pKa = 6.01 FNLFLTSEE182 pKa = 3.94 DD183 pKa = 3.33 RR184 pKa = 11.84 FYY186 pKa = 11.1 QGQSFNFSYY195 pKa = 10.13 FYY197 pKa = 10.71 EE198 pKa = 4.63 DD199 pKa = 3.52 RR200 pKa = 11.84 DD201 pKa = 3.6 INAGKK206 pKa = 9.59 EE207 pKa = 4.01 AEE209 pKa = 4.15 IKK211 pKa = 10.51 ILGIDD216 pKa = 3.25 RR217 pKa = 11.84 QYY219 pKa = 11.69 FDD221 pKa = 3.92 YY222 pKa = 10.34 STILIEE228 pKa = 5.09 QSEE231 pKa = 4.3 QDD233 pKa = 3.49 GGNPFQPPPVRR244 pKa = 11.84 IRR246 pKa = 11.84 SNIINTTNPDD256 pKa = 3.5 NYY258 pKa = 11.33 ALGYY262 pKa = 10.25 FNLSEE267 pKa = 4.34 TYY269 pKa = 10.72 SFLFTFQEE277 pKa = 4.07 QQ278 pKa = 3.09
Molecular weight: 31.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.689
IPC2_protein 3.706
IPC_protein 3.719
Toseland 3.503
ProMoST 3.884
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.656
Rodwell 3.541
Grimsley 3.401
Solomon 3.706
Lehninger 3.668
Nozaki 3.834
DTASelect 4.075
Thurlkill 3.554
EMBOSS 3.668
Sillero 3.846
Patrickios 1.202
IPC_peptide 3.706
IPC2_peptide 3.821
IPC2.peptide.svr19 3.758
Protein with the highest isoelectric point:
>tr|A0A5B1B607|A0A5B1B607_9FLAO Peroxidase-related enzyme OS=Aquimarina sp. RZ0 OX=2607730 GN=F0000_24850 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 10.15 RR10 pKa = 11.84 KK11 pKa = 9.6 RR12 pKa = 11.84 KK13 pKa = 8.29 NKK15 pKa = 9.34 HH16 pKa = 4.03 GFRR19 pKa = 11.84 EE20 pKa = 4.2 RR21 pKa = 11.84 MASANGRR28 pKa = 11.84 KK29 pKa = 9.04 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.09 GRR39 pKa = 11.84 KK40 pKa = 7.97 KK41 pKa = 10.66 LSVSSEE47 pKa = 3.9 LRR49 pKa = 11.84 HH50 pKa = 5.43 KK51 pKa = 10.29 HH52 pKa = 5.04
Molecular weight: 6.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.33
IPC2_protein 10.716
IPC_protein 12.266
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.281
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 12.003
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.051
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5315
0
5315
1900794
27
6599
357.6
40.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.698 ± 0.029
0.792 ± 0.01
5.762 ± 0.039
6.44 ± 0.034
5.111 ± 0.029
6.391 ± 0.041
1.779 ± 0.015
8.632 ± 0.033
7.759 ± 0.053
8.967 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.964 ± 0.016
6.324 ± 0.037
3.364 ± 0.02
3.465 ± 0.018
3.537 ± 0.025
6.846 ± 0.031
6.132 ± 0.037
5.804 ± 0.03
1.091 ± 0.013
4.141 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here