Pseudomonas phage 201phi2-1 (Pseudomonas chlororaphis phage 201phi2-1)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Phikzvirus; unclassified Phikzvirus

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 461 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B3FJ39|B3FJ39_BP201 Uncharacterized protein OS=Pseudomonas phage 201phi2-1 OX=198110 GN=201phi2-1p176 PE=4 SV=1
MM1 pKa = 7.66AYY3 pKa = 9.96VNRR6 pKa = 11.84RR7 pKa = 11.84QMHH10 pKa = 5.28MEE12 pKa = 3.77QYY14 pKa = 10.58FSDD17 pKa = 4.12RR18 pKa = 11.84EE19 pKa = 4.14KK20 pKa = 11.25LDD22 pKa = 3.49APYY25 pKa = 9.8WAIDD29 pKa = 3.92DD30 pKa = 4.15NLDD33 pKa = 4.28SIVCNVRR40 pKa = 11.84DD41 pKa = 3.82PFATYY46 pKa = 10.79VDD48 pKa = 3.84PSNYY52 pKa = 10.27DD53 pKa = 3.5DD54 pKa = 5.49DD55 pKa = 5.15ALAYY59 pKa = 9.42RR60 pKa = 11.84EE61 pKa = 4.23CMDD64 pKa = 3.49GVVAGVSEE72 pKa = 4.62ALGLPQGLSFEE83 pKa = 4.62EE84 pKa = 4.31VFEE87 pKa = 4.32RR88 pKa = 11.84LL89 pKa = 3.33

Molecular weight: 10.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B3FK07|B3FK07_BP201 Major virion structural protein OS=Pseudomonas phage 201phi2-1 OX=198110 GN=201phi2-1p032 PE=4 SV=1
MM1 pKa = 7.57AKK3 pKa = 10.37KK4 pKa = 8.77LTKK7 pKa = 10.13GKK9 pKa = 10.12RR10 pKa = 11.84KK11 pKa = 9.96RR12 pKa = 11.84LMTLVTEE19 pKa = 4.65WALCRR24 pKa = 11.84LIAKK28 pKa = 9.56KK29 pKa = 9.76HH30 pKa = 4.84VQDD33 pKa = 4.04AQRR36 pKa = 11.84QAITNEE42 pKa = 3.99RR43 pKa = 11.84QRR45 pKa = 11.84RR46 pKa = 11.84AYY48 pKa = 8.15MAQYY52 pKa = 10.39CC53 pKa = 3.97

Molecular weight: 6.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

461

0

461

99033

29

2337

214.8

24.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.129 ± 0.161

0.978 ± 0.057

6.554 ± 0.107

6.529 ± 0.141

4.02 ± 0.091

6.462 ± 0.164

2.166 ± 0.079

6.119 ± 0.097

5.55 ± 0.138

8.456 ± 0.107

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.856 ± 0.058

4.987 ± 0.076

4.379 ± 0.076

3.798 ± 0.094

5.19 ± 0.091

5.556 ± 0.096

6.434 ± 0.111

7.22 ± 0.106

1.465 ± 0.048

4.151 ± 0.088
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski