Oceanospirillales bacterium LUC14_002_19_P2

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; unclassified Oceanospirillales

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2898 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y1R773|A0A1Y1R773_9GAMM Phosphoribosylamine--glycine ligase OS=Oceanospirillales bacterium LUC14_002_19_P2 OX=1940822 GN=purD PE=3 SV=1
MM1 pKa = 6.68TTDD4 pKa = 3.19ASGLAPVSFDD14 pKa = 3.18RR15 pKa = 11.84LSNLFLEE22 pKa = 4.73LQSYY26 pKa = 8.61QSPSEE31 pKa = 3.94LHH33 pKa = 6.71GLLCGQYY40 pKa = 10.43CAGQRR45 pKa = 11.84PAHH48 pKa = 5.75GAWLATAAEE57 pKa = 4.47HH58 pKa = 6.01MAVTQTLDD66 pKa = 3.21AAAKK70 pKa = 10.08AVLLQLYY77 pKa = 10.33DD78 pKa = 3.6DD79 pKa = 4.57TLGKK83 pKa = 9.93LQSEE87 pKa = 5.13DD88 pKa = 3.96YY89 pKa = 10.87SFQPLLPDD97 pKa = 4.14DD98 pKa = 5.63DD99 pKa = 4.37EE100 pKa = 6.64ALGQRR105 pKa = 11.84TQALGQWCQGFLGGYY120 pKa = 8.05GLSGRR125 pKa = 11.84SEE127 pKa = 4.16ADD129 pKa = 2.92LSEE132 pKa = 4.23EE133 pKa = 4.2VASILTDD140 pKa = 4.18FASIAQVQFDD150 pKa = 4.69DD151 pKa = 5.55LDD153 pKa = 4.34DD154 pKa = 4.89SEE156 pKa = 6.09ASEE159 pKa = 4.38KK160 pKa = 11.14DD161 pKa = 3.34FLEE164 pKa = 4.14VSEE167 pKa = 4.34YY168 pKa = 11.12LRR170 pKa = 11.84MATLMLFGEE179 pKa = 4.76SEE181 pKa = 4.28GSSEE185 pKa = 4.53AEE187 pKa = 3.9AGQSTGSLHH196 pKa = 6.67

Molecular weight: 21.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y1R3H3|A0A1Y1R3H3_9GAMM Methionine synthase OS=Oceanospirillales bacterium LUC14_002_19_P2 OX=1940822 GN=B0D91_10250 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.97RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.35SGQQVIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.92SRR39 pKa = 11.84KK40 pKa = 9.1RR41 pKa = 11.84LSAA44 pKa = 3.74

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2898

0

2898

894931

26

8644

308.8

34.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.1 ± 0.044

1.126 ± 0.021

5.895 ± 0.062

6.18 ± 0.052

3.684 ± 0.031

7.401 ± 0.077

2.299 ± 0.024

5.768 ± 0.035

4.041 ± 0.043

10.656 ± 0.071

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.69 ± 0.033

3.647 ± 0.037

4.4 ± 0.043

4.425 ± 0.036

5.884 ± 0.054

6.152 ± 0.041

5.579 ± 0.06

7.011 ± 0.043

1.286 ± 0.022

2.778 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski