Pneumocystis jirovecii (strain SE8) (Human pneumocystis pneumonia agent)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Pneumocystidomycetes; Pneumocystidales; Pneumocystidaceae; Pneumocystis; Pneumocystis jirovecii

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3419 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L0P9B4|L0P9B4_PNEJ8 Uncharacterized protein OS=Pneumocystis jirovecii (strain SE8) OX=1209962 GN=PNEJI1_002589 PE=3 SV=1
MM1 pKa = 7.43SFYY4 pKa = 11.25DD5 pKa = 4.0EE6 pKa = 5.34IEE8 pKa = 4.56IEE10 pKa = 5.03DD11 pKa = 3.75FTFDD15 pKa = 3.35TEE17 pKa = 4.2LQIYY21 pKa = 8.98HH22 pKa = 5.9YY23 pKa = 9.19PCPCGDD29 pKa = 3.35RR30 pKa = 11.84FEE32 pKa = 5.82ISLNDD37 pKa = 3.47LRR39 pKa = 11.84KK40 pKa = 9.65GDD42 pKa = 4.37EE43 pKa = 3.73IAYY46 pKa = 9.97CPSCSLMVRR55 pKa = 11.84VIYY58 pKa = 10.58DD59 pKa = 3.06QDD61 pKa = 3.41KK62 pKa = 10.55FLEE65 pKa = 4.34NDD67 pKa = 4.14DD68 pKa = 4.82EE69 pKa = 5.89ISLQSAPVIVVV80 pKa = 3.47

Molecular weight: 9.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L0P9M7|L0P9M7_PNEJ8 Rab-GAP TBC domain-containing protein OS=Pneumocystis jirovecii (strain SE8) OX=1209962 GN=PNEJI1_001984 PE=4 SV=1
MM1 pKa = 7.9PGPTVSRR8 pKa = 11.84RR9 pKa = 11.84SSRR12 pKa = 11.84LTVLKK17 pKa = 10.94NSGGKK22 pKa = 9.27FLPALFYY29 pKa = 11.32SPVSLIFPVFFPPFPPLSDD48 pKa = 3.93PSADD52 pKa = 3.43APQDD56 pKa = 3.48IQQHH60 pKa = 5.59RR61 pKa = 11.84KK62 pKa = 9.43GLPASPKK69 pKa = 9.52EE70 pKa = 3.94RR71 pKa = 11.84LPPFQDD77 pKa = 3.02GCPPRR82 pKa = 11.84RR83 pKa = 11.84PPRR86 pKa = 11.84RR87 pKa = 11.84QHH89 pKa = 5.94RR90 pKa = 11.84RR91 pKa = 11.84ALAPYY96 pKa = 8.77CINVSIATTTDD107 pKa = 2.91ASDD110 pKa = 3.58VGGDD114 pKa = 2.97WRR116 pKa = 11.84DD117 pKa = 2.94ARR119 pKa = 11.84EE120 pKa = 3.97RR121 pKa = 11.84GRR123 pKa = 11.84QRR125 pKa = 11.84GDD127 pKa = 2.77GRR129 pKa = 11.84RR130 pKa = 11.84CQYY133 pKa = 9.55TQGLCAPRR141 pKa = 11.84PVDD144 pKa = 3.44ALHH147 pKa = 6.19EE148 pKa = 4.21QRR150 pKa = 11.84GCPPCNRR157 pKa = 11.84VCPVLHH163 pKa = 6.75

Molecular weight: 18.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3419

0

3419

1381820

13

5307

404.2

46.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.615 ± 0.034

1.541 ± 0.015

5.414 ± 0.027

6.782 ± 0.037

4.768 ± 0.029

4.426 ± 0.036

2.302 ± 0.016

8.327 ± 0.049

8.076 ± 0.043

9.772 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.141 ± 0.016

6.036 ± 0.034

3.786 ± 0.028

3.762 ± 0.028

4.471 ± 0.032

8.697 ± 0.045

5.203 ± 0.027

5.073 ± 0.033

1.106 ± 0.012

3.702 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski