Gordonia phage Demosthenes

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Demosthenesvirus; Gordonia virus Demosthenes

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A160DGK9|A0A160DGK9_9CAUD Uncharacterized protein OS=Gordonia phage Demosthenes OX=1838067 GN=95 PE=4 SV=1
MM1 pKa = 7.25NPWLYY6 pKa = 10.76ALQVAVILTILVVAVIIAVAGVCEE30 pKa = 3.9ARR32 pKa = 11.84EE33 pKa = 4.23WEE35 pKa = 4.32DD36 pKa = 3.87DD37 pKa = 3.44

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A166Y6Z1|A0A166Y6Z1_9CAUD Uncharacterized protein OS=Gordonia phage Demosthenes OX=1838067 GN=84 PE=4 SV=1
MM1 pKa = 8.24RR2 pKa = 11.84DD3 pKa = 3.31TQEE6 pKa = 4.33NIQVRR11 pKa = 11.84IMLARR16 pKa = 11.84KK17 pKa = 9.29KK18 pKa = 9.82CTGNSSDD25 pKa = 3.68HH26 pKa = 6.92ANGKK30 pKa = 8.67HH31 pKa = 4.46VAASRR36 pKa = 11.84LLYY39 pKa = 10.29RR40 pKa = 11.84AVLTGCRR47 pKa = 11.84PNYY50 pKa = 9.21EE51 pKa = 3.76GRR53 pKa = 11.84HH54 pKa = 4.57RR55 pKa = 11.84RR56 pKa = 11.84EE57 pKa = 3.71

Molecular weight: 6.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

95

0

95

23282

27

3028

245.1

27.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.157 ± 0.585

0.996 ± 0.151

6.245 ± 0.285

6.941 ± 0.404

3.501 ± 0.144

7.727 ± 0.301

1.984 ± 0.166

5.429 ± 0.209

5.236 ± 0.23

7.358 ± 0.265

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.826 ± 0.179

3.934 ± 0.118

5.335 ± 0.313

3.874 ± 0.155

5.725 ± 0.375

5.979 ± 0.248

6.039 ± 0.221

6.795 ± 0.205

1.8 ± 0.094

3.118 ± 0.193
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski