Amino acid dipepetide frequency for Gordonia phage Demosthenes

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.524AlaAla: 10.524 ± 1.493
0.687AlaCys: 0.687 ± 0.252
5.326AlaAsp: 5.326 ± 0.425
5.283AlaGlu: 5.283 ± 0.616
3.565AlaPhe: 3.565 ± 0.396
7.56AlaGly: 7.56 ± 0.707
1.589AlaHis: 1.589 ± 0.328
5.627AlaIle: 5.627 ± 0.688
4.553AlaLys: 4.553 ± 0.561
7.646AlaLeu: 7.646 ± 1.178
3.007AlaMet: 3.007 ± 0.654
2.921AlaAsn: 2.921 ± 0.433
3.78AlaPro: 3.78 ± 0.451
3.479AlaGln: 3.479 ± 0.328
5.885AlaArg: 5.885 ± 0.461
4.725AlaSer: 4.725 ± 0.518
5.069AlaThr: 5.069 ± 0.505
6.4AlaVal: 6.4 ± 0.552
1.632AlaTrp: 1.632 ± 0.457
2.319AlaTyr: 2.319 ± 0.331
0.0AlaXaa: 0.0 ± 0.0
Cys
0.472CysAla: 0.472 ± 0.162
0.086CysCys: 0.086 ± 0.066
0.687CysAsp: 0.687 ± 0.208
0.601CysGlu: 0.601 ± 0.152
0.172CysPhe: 0.172 ± 0.083
0.73CysGly: 0.73 ± 0.191
0.172CysHis: 0.172 ± 0.084
0.601CysIle: 0.601 ± 0.177
0.472CysLys: 0.472 ± 0.166
0.73CysLeu: 0.73 ± 0.189
0.258CysMet: 0.258 ± 0.107
0.43CysAsn: 0.43 ± 0.154
0.644CysPro: 0.644 ± 0.202
0.258CysGln: 0.258 ± 0.107
0.816CysArg: 0.816 ± 0.212
0.73CysSer: 0.73 ± 0.194
0.816CysThr: 0.816 ± 0.206
0.515CysVal: 0.515 ± 0.171
0.472CysTrp: 0.472 ± 0.152
0.301CysTyr: 0.301 ± 0.122
0.0CysXaa: 0.0 ± 0.0
Asp
5.326AspAla: 5.326 ± 0.604
0.472AspCys: 0.472 ± 0.142
4.381AspAsp: 4.381 ± 0.714
5.455AspGlu: 5.455 ± 0.638
2.405AspPhe: 2.405 ± 0.363
5.026AspGly: 5.026 ± 0.498
1.503AspHis: 1.503 ± 0.249
2.835AspIle: 2.835 ± 0.345
2.749AspLys: 2.749 ± 0.323
4.338AspLeu: 4.338 ± 0.461
1.074AspMet: 1.074 ± 0.166
2.019AspAsn: 2.019 ± 0.289
4.725AspPro: 4.725 ± 0.574
1.847AspGln: 1.847 ± 0.216
3.264AspArg: 3.264 ± 0.45
3.952AspSer: 3.952 ± 0.336
3.35AspThr: 3.35 ± 0.429
4.038AspVal: 4.038 ± 0.448
1.203AspTrp: 1.203 ± 0.252
2.491AspTyr: 2.491 ± 0.367
0.0AspXaa: 0.0 ± 0.0
Glu
5.67GluAla: 5.67 ± 0.506
0.515GluCys: 0.515 ± 0.138
4.381GluAsp: 4.381 ± 0.56
4.166GluGlu: 4.166 ± 0.568
2.792GluPhe: 2.792 ± 0.36
4.94GluGly: 4.94 ± 0.457
1.246GluHis: 1.246 ± 0.286
4.124GluIle: 4.124 ± 0.381
3.307GluLys: 3.307 ± 0.471
6.013GluLeu: 6.013 ± 0.633
2.706GluMet: 2.706 ± 0.347
2.405GluAsn: 2.405 ± 0.391
3.608GluPro: 3.608 ± 0.631
3.179GluGln: 3.179 ± 0.379
3.995GluArg: 3.995 ± 0.516
4.252GluSer: 4.252 ± 0.321
3.35GluThr: 3.35 ± 0.419
4.94GluVal: 4.94 ± 0.614
1.332GluTrp: 1.332 ± 0.228
2.491GluTyr: 2.491 ± 0.339
0.0GluXaa: 0.0 ± 0.0
Phe
3.35PheAla: 3.35 ± 0.567
0.472PheCys: 0.472 ± 0.132
2.577PheAsp: 2.577 ± 0.287
2.921PheGlu: 2.921 ± 0.379
1.074PhePhe: 1.074 ± 0.291
2.62PheGly: 2.62 ± 0.292
0.859PheHis: 0.859 ± 0.203
1.589PheIle: 1.589 ± 0.254
1.718PheLys: 1.718 ± 0.365
2.234PheLeu: 2.234 ± 0.388
0.945PheMet: 0.945 ± 0.266
1.632PheAsn: 1.632 ± 0.22
1.46PhePro: 1.46 ± 0.289
1.417PheGln: 1.417 ± 0.334
1.761PheArg: 1.761 ± 0.292
2.448PheSer: 2.448 ± 0.315
2.277PheThr: 2.277 ± 0.308
2.191PheVal: 2.191 ± 0.237
0.515PheTrp: 0.515 ± 0.135
0.945PheTyr: 0.945 ± 0.259
0.0PheXaa: 0.0 ± 0.0
Gly
6.357GlyAla: 6.357 ± 0.741
0.73GlyCys: 0.73 ± 0.245
4.038GlyAsp: 4.038 ± 0.445
4.897GlyGlu: 4.897 ± 0.454
2.878GlyPhe: 2.878 ± 0.399
5.928GlyGly: 5.928 ± 0.621
1.546GlyHis: 1.546 ± 0.282
4.209GlyIle: 4.209 ± 0.623
5.026GlyLys: 5.026 ± 0.511
5.197GlyLeu: 5.197 ± 0.489
3.05GlyMet: 3.05 ± 0.574
2.706GlyAsn: 2.706 ± 0.321
3.737GlyPro: 3.737 ± 0.397
3.05GlyGln: 3.05 ± 0.367
4.338GlyArg: 4.338 ± 0.5
5.283GlySer: 5.283 ± 0.599
4.897GlyThr: 4.897 ± 0.431
5.455GlyVal: 5.455 ± 0.416
1.46GlyTrp: 1.46 ± 0.208
2.491GlyTyr: 2.491 ± 0.438
0.0GlyXaa: 0.0 ± 0.0
His
1.417HisAla: 1.417 ± 0.268
0.301HisCys: 0.301 ± 0.15
1.117HisAsp: 1.117 ± 0.272
2.191HisGlu: 2.191 ± 0.397
0.644HisPhe: 0.644 ± 0.182
1.589HisGly: 1.589 ± 0.327
0.258HisHis: 0.258 ± 0.104
1.074HisIle: 1.074 ± 0.247
1.117HisLys: 1.117 ± 0.254
1.074HisLeu: 1.074 ± 0.223
0.644HisMet: 0.644 ± 0.173
0.387HisAsn: 0.387 ± 0.131
1.375HisPro: 1.375 ± 0.305
0.73HisGln: 0.73 ± 0.212
1.16HisArg: 1.16 ± 0.276
1.417HisSer: 1.417 ± 0.227
0.945HisThr: 0.945 ± 0.173
1.289HisVal: 1.289 ± 0.214
0.301HisTrp: 0.301 ± 0.093
0.816HisTyr: 0.816 ± 0.198
0.0HisXaa: 0.0 ± 0.0
Ile
5.326IleAla: 5.326 ± 0.519
0.387IleCys: 0.387 ± 0.156
3.909IleAsp: 3.909 ± 0.454
4.768IleGlu: 4.768 ± 0.487
1.976IlePhe: 1.976 ± 0.236
4.811IleGly: 4.811 ± 0.537
1.031IleHis: 1.031 ± 0.269
3.479IleIle: 3.479 ± 0.392
3.436IleLys: 3.436 ± 0.432
3.651IleLeu: 3.651 ± 0.482
1.289IleMet: 1.289 ± 0.236
2.277IleAsn: 2.277 ± 0.289
2.019IlePro: 2.019 ± 0.325
2.062IleGln: 2.062 ± 0.302
2.878IleArg: 2.878 ± 0.356
3.007IleSer: 3.007 ± 0.348
3.436IleThr: 3.436 ± 0.427
2.577IleVal: 2.577 ± 0.378
0.773IleTrp: 0.773 ± 0.217
1.203IleTyr: 1.203 ± 0.234
0.0IleXaa: 0.0 ± 0.0
Lys
5.154LysAla: 5.154 ± 0.363
0.558LysCys: 0.558 ± 0.18
2.749LysAsp: 2.749 ± 0.326
3.307LysGlu: 3.307 ± 0.465
2.105LysPhe: 2.105 ± 0.341
3.35LysGly: 3.35 ± 0.468
1.031LysHis: 1.031 ± 0.24
2.491LysIle: 2.491 ± 0.375
3.264LysLys: 3.264 ± 0.419
4.639LysLeu: 4.639 ± 0.637
1.332LysMet: 1.332 ± 0.288
1.933LysAsn: 1.933 ± 0.315
2.706LysPro: 2.706 ± 0.529
1.847LysGln: 1.847 ± 0.313
3.393LysArg: 3.393 ± 0.457
3.179LysSer: 3.179 ± 0.4
2.534LysThr: 2.534 ± 0.316
4.038LysVal: 4.038 ± 0.447
0.816LysTrp: 0.816 ± 0.159
1.933LysTyr: 1.933 ± 0.346
0.0LysXaa: 0.0 ± 0.0
Leu
6.228LeuAla: 6.228 ± 0.421
1.117LeuCys: 1.117 ± 0.298
4.725LeuAsp: 4.725 ± 0.533
5.67LeuGlu: 5.67 ± 0.456
2.277LeuPhe: 2.277 ± 0.309
5.971LeuGly: 5.971 ± 0.776
1.417LeuHis: 1.417 ± 0.234
3.866LeuIle: 3.866 ± 0.406
3.78LeuLys: 3.78 ± 0.574
5.026LeuLeu: 5.026 ± 0.517
2.362LeuMet: 2.362 ± 0.322
2.835LeuAsn: 2.835 ± 0.327
5.154LeuPro: 5.154 ± 0.563
2.749LeuGln: 2.749 ± 0.284
5.197LeuArg: 5.197 ± 0.487
3.823LeuSer: 3.823 ± 0.46
3.78LeuThr: 3.78 ± 0.453
4.768LeuVal: 4.768 ± 0.447
0.988LeuTrp: 0.988 ± 0.194
1.847LeuTyr: 1.847 ± 0.332
0.0LeuXaa: 0.0 ± 0.0
Met
3.264MetAla: 3.264 ± 0.442
0.258MetCys: 0.258 ± 0.116
1.246MetAsp: 1.246 ± 0.212
1.804MetGlu: 1.804 ± 0.282
0.988MetPhe: 0.988 ± 0.212
1.804MetGly: 1.804 ± 0.254
0.773MetHis: 0.773 ± 0.183
1.417MetIle: 1.417 ± 0.436
1.289MetLys: 1.289 ± 0.257
1.675MetLeu: 1.675 ± 0.366
0.816MetMet: 0.816 ± 0.221
1.46MetAsn: 1.46 ± 0.364
1.375MetPro: 1.375 ± 0.248
1.847MetGln: 1.847 ± 0.389
1.16MetArg: 1.16 ± 0.231
2.234MetSer: 2.234 ± 0.297
2.105MetThr: 2.105 ± 0.318
1.632MetVal: 1.632 ± 0.284
0.387MetTrp: 0.387 ± 0.1
0.73MetTyr: 0.73 ± 0.189
0.0MetXaa: 0.0 ± 0.0
Asn
3.823AsnAla: 3.823 ± 0.479
0.387AsnCys: 0.387 ± 0.124
2.019AsnAsp: 2.019 ± 0.33
2.491AsnGlu: 2.491 ± 0.332
1.546AsnPhe: 1.546 ± 0.273
3.093AsnGly: 3.093 ± 0.319
0.773AsnHis: 0.773 ± 0.171
2.019AsnIle: 2.019 ± 0.344
2.319AsnLys: 2.319 ± 0.307
2.663AsnLeu: 2.663 ± 0.308
0.73AsnMet: 0.73 ± 0.166
1.761AsnAsn: 1.761 ± 0.28
2.405AsnPro: 2.405 ± 0.313
1.632AsnGln: 1.632 ± 0.258
2.148AsnArg: 2.148 ± 0.402
2.105AsnSer: 2.105 ± 0.355
2.706AsnThr: 2.706 ± 0.332
2.835AsnVal: 2.835 ± 0.423
0.472AsnTrp: 0.472 ± 0.16
1.417AsnTyr: 1.417 ± 0.312
0.0AsnXaa: 0.0 ± 0.0
Pro
5.069ProAla: 5.069 ± 0.619
0.215ProCys: 0.215 ± 0.13
4.124ProAsp: 4.124 ± 0.582
4.768ProGlu: 4.768 ± 0.617
1.718ProPhe: 1.718 ± 0.31
4.166ProGly: 4.166 ± 0.448
0.773ProHis: 0.773 ± 0.165
2.706ProIle: 2.706 ± 0.274
2.319ProLys: 2.319 ± 0.476
3.393ProLeu: 3.393 ± 0.338
1.203ProMet: 1.203 ± 0.227
2.062ProAsn: 2.062 ± 0.284
3.007ProPro: 3.007 ± 0.532
1.976ProGln: 1.976 ± 0.256
2.448ProArg: 2.448 ± 0.419
3.307ProSer: 3.307 ± 0.445
3.737ProThr: 3.737 ± 0.537
3.823ProVal: 3.823 ± 0.421
1.16ProTrp: 1.16 ± 0.261
1.375ProTyr: 1.375 ± 0.211
0.0ProXaa: 0.0 ± 0.0
Gln
4.209GlnAla: 4.209 ± 0.564
0.472GlnCys: 0.472 ± 0.179
1.718GlnAsp: 1.718 ± 0.252
1.976GlnGlu: 1.976 ± 0.318
1.332GlnPhe: 1.332 ± 0.237
3.007GlnGly: 3.007 ± 0.42
1.117GlnHis: 1.117 ± 0.224
1.933GlnIle: 1.933 ± 0.256
1.847GlnLys: 1.847 ± 0.28
3.565GlnLeu: 3.565 ± 0.364
0.73GlnMet: 0.73 ± 0.175
1.46GlnAsn: 1.46 ± 0.259
1.804GlnPro: 1.804 ± 0.313
1.375GlnGln: 1.375 ± 0.275
2.878GlnArg: 2.878 ± 0.349
1.976GlnSer: 1.976 ± 0.256
2.105GlnThr: 2.105 ± 0.304
3.136GlnVal: 3.136 ± 0.331
1.16GlnTrp: 1.16 ± 0.249
0.945GlnTyr: 0.945 ± 0.179
0.0GlnXaa: 0.0 ± 0.0
Arg
5.069ArgAla: 5.069 ± 0.553
0.773ArgCys: 0.773 ± 0.238
3.179ArgAsp: 3.179 ± 0.481
3.823ArgGlu: 3.823 ± 0.535
1.761ArgPhe: 1.761 ± 0.235
3.651ArgGly: 3.651 ± 0.478
1.332ArgHis: 1.332 ± 0.24
3.393ArgIle: 3.393 ± 0.47
3.35ArgLys: 3.35 ± 0.519
4.467ArgLeu: 4.467 ± 0.418
2.148ArgMet: 2.148 ± 0.337
2.491ArgAsn: 2.491 ± 0.42
3.264ArgPro: 3.264 ± 0.473
1.976ArgGln: 1.976 ± 0.247
3.307ArgArg: 3.307 ± 0.699
2.792ArgSer: 2.792 ± 0.29
3.007ArgThr: 3.007 ± 0.315
4.252ArgVal: 4.252 ± 0.488
1.074ArgTrp: 1.074 ± 0.23
2.105ArgTyr: 2.105 ± 0.298
0.0ArgXaa: 0.0 ± 0.0
Ser
5.069SerAla: 5.069 ± 0.511
0.687SerCys: 0.687 ± 0.231
3.866SerAsp: 3.866 ± 0.525
2.964SerGlu: 2.964 ± 0.455
1.804SerPhe: 1.804 ± 0.259
5.326SerGly: 5.326 ± 0.474
1.117SerHis: 1.117 ± 0.273
3.179SerIle: 3.179 ± 0.378
3.179SerLys: 3.179 ± 0.317
4.553SerLeu: 4.553 ± 0.483
1.589SerMet: 1.589 ± 0.271
2.878SerAsn: 2.878 ± 0.303
3.179SerPro: 3.179 ± 0.468
2.148SerGln: 2.148 ± 0.236
2.534SerArg: 2.534 ± 0.415
3.694SerSer: 3.694 ± 0.432
4.424SerThr: 4.424 ± 0.499
4.295SerVal: 4.295 ± 0.438
0.816SerTrp: 0.816 ± 0.191
2.491SerTyr: 2.491 ± 0.411
0.0SerXaa: 0.0 ± 0.0
Thr
5.713ThrAla: 5.713 ± 0.703
0.344ThrCys: 0.344 ± 0.14
4.038ThrAsp: 4.038 ± 0.531
3.737ThrGlu: 3.737 ± 0.445
1.933ThrPhe: 1.933 ± 0.252
5.24ThrGly: 5.24 ± 0.463
0.945ThrHis: 0.945 ± 0.26
2.835ThrIle: 2.835 ± 0.368
3.479ThrLys: 3.479 ± 0.353
4.682ThrLeu: 4.682 ± 0.442
1.332ThrMet: 1.332 ± 0.246
2.277ThrAsn: 2.277 ± 0.347
3.479ThrPro: 3.479 ± 0.494
2.362ThrGln: 2.362 ± 0.29
3.136ThrArg: 3.136 ± 0.336
3.952ThrSer: 3.952 ± 0.461
3.78ThrThr: 3.78 ± 0.453
3.823ThrVal: 3.823 ± 0.457
1.46ThrTrp: 1.46 ± 0.257
1.847ThrTyr: 1.847 ± 0.385
0.0ThrXaa: 0.0 ± 0.0
Val
5.799ValAla: 5.799 ± 0.511
0.773ValCys: 0.773 ± 0.206
4.725ValAsp: 4.725 ± 0.435
4.983ValGlu: 4.983 ± 0.44
2.277ValPhe: 2.277 ± 0.277
4.725ValGly: 4.725 ± 0.613
1.289ValHis: 1.289 ± 0.262
4.252ValIle: 4.252 ± 0.436
3.222ValLys: 3.222 ± 0.434
4.081ValLeu: 4.081 ± 0.416
1.589ValMet: 1.589 ± 0.24
2.792ValAsn: 2.792 ± 0.328
3.78ValPro: 3.78 ± 0.333
3.222ValGln: 3.222 ± 0.401
3.823ValArg: 3.823 ± 0.515
4.081ValSer: 4.081 ± 0.447
4.725ValThr: 4.725 ± 0.554
4.725ValVal: 4.725 ± 0.552
1.203ValTrp: 1.203 ± 0.228
1.89ValTyr: 1.89 ± 0.291
0.0ValXaa: 0.0 ± 0.0
Trp
1.246TrpAla: 1.246 ± 0.323
0.301TrpCys: 0.301 ± 0.111
1.332TrpAsp: 1.332 ± 0.326
1.332TrpGlu: 1.332 ± 0.238
0.859TrpPhe: 0.859 ± 0.208
1.289TrpGly: 1.289 ± 0.225
0.43TrpHis: 0.43 ± 0.137
0.988TrpIle: 0.988 ± 0.22
0.601TrpLys: 0.601 ± 0.164
1.246TrpLeu: 1.246 ± 0.173
0.558TrpMet: 0.558 ± 0.147
0.902TrpAsn: 0.902 ± 0.213
0.859TrpPro: 0.859 ± 0.186
0.558TrpGln: 0.558 ± 0.138
1.203TrpArg: 1.203 ± 0.201
1.203TrpSer: 1.203 ± 0.236
1.16TrpThr: 1.16 ± 0.231
1.246TrpVal: 1.246 ± 0.202
0.172TrpTrp: 0.172 ± 0.081
0.515TrpTyr: 0.515 ± 0.175
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.491TyrAla: 2.491 ± 0.398
0.43TyrCys: 0.43 ± 0.144
2.319TyrAsp: 2.319 ± 0.335
2.277TyrGlu: 2.277 ± 0.373
0.902TyrPhe: 0.902 ± 0.189
2.448TyrGly: 2.448 ± 0.328
0.644TyrHis: 0.644 ± 0.207
1.675TyrIle: 1.675 ± 0.308
1.332TyrLys: 1.332 ± 0.319
2.706TyrLeu: 2.706 ± 0.406
0.859TyrMet: 0.859 ± 0.263
1.718TyrAsn: 1.718 ± 0.201
0.988TyrPro: 0.988 ± 0.245
1.031TyrGln: 1.031 ± 0.224
1.933TyrArg: 1.933 ± 0.318
1.632TyrSer: 1.632 ± 0.272
2.191TyrThr: 2.191 ± 0.294
1.976TyrVal: 1.976 ± 0.307
0.601TyrTrp: 0.601 ± 0.148
1.031TyrTyr: 1.031 ± 0.21
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 95 proteins (23282 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski