Mycobacterium phage EvilGenius

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 93 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A143FRN2|A0A143FRN2_9CAUD Uncharacterized protein OS=Mycobacterium phage EvilGenius OX=1821723 GN=87 PE=4 SV=1
MM1 pKa = 7.56MDD3 pKa = 3.73DD4 pKa = 3.34QNVYY8 pKa = 10.35VVVDD12 pKa = 3.51PDD14 pKa = 3.89GRR16 pKa = 11.84LLPYY20 pKa = 9.75IAYY23 pKa = 9.73GIDD26 pKa = 3.51GAIEE30 pKa = 3.68QHH32 pKa = 5.46QEE34 pKa = 3.6NDD36 pKa = 3.12GSEE39 pKa = 3.87IEE41 pKa = 4.69GIFVYY46 pKa = 10.42GSPRR50 pKa = 11.84VGLRR54 pKa = 3.04

Molecular weight: 6.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A143FQN4|A0A143FQN4_9CAUD Uncharacterized protein OS=Mycobacterium phage EvilGenius OX=1821723 GN=59 PE=4 SV=1
MM1 pKa = 7.53GWTTNPSPIITPKK14 pKa = 10.06SKK16 pKa = 9.72GWSVSPTAPSPSAEE30 pKa = 4.21LGWWAVVGIDD40 pKa = 4.31TNLTVQFVTKK50 pKa = 10.45TEE52 pKa = 3.9LTAIKK57 pKa = 10.33QLGLVQAVTATRR69 pKa = 11.84DD70 pKa = 3.28LAFQAVKK77 pKa = 9.79MLTVNAQIGATRR89 pKa = 11.84NLGFSSVQNMSANQQVGLTPSLGFGFPPNGPLPQTFTTAIFTYY132 pKa = 8.77TIPRR136 pKa = 11.84WCNYY140 pKa = 9.4IDD142 pKa = 4.18VILLGGGGGGGGGASTTQSGAGGGPGSWLTVTLEE176 pKa = 4.22RR177 pKa = 11.84GVHH180 pKa = 5.56IPFSLVSFVGQLGTGGTGANGGITPTTGGNGNPTVIPSLSLSAAGGIGSASSNQDD235 pKa = 2.54GMSLEE240 pKa = 4.03RR241 pKa = 11.84RR242 pKa = 11.84RR243 pKa = 11.84TTASPTQAAQRR254 pKa = 11.84SLPATAQTATHH265 pKa = 6.9RR266 pKa = 11.84AAAHH270 pKa = 5.94AAVTTASSSPAQRR283 pKa = 11.84AATVASARR291 pKa = 11.84RR292 pKa = 11.84GSAHH296 pKa = 5.49TNKK299 pKa = 9.72EE300 pKa = 4.05NSWQQVV306 pKa = 2.91

Molecular weight: 31.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

93

0

93

16797

27

847

180.6

19.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.877 ± 0.326

0.917 ± 0.121

6.442 ± 0.219

6.68 ± 0.309

3.566 ± 0.183

8.275 ± 0.358

1.965 ± 0.16

4.989 ± 0.161

4.787 ± 0.234

8.323 ± 0.306

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.399 ± 0.131

3.262 ± 0.131

5.477 ± 0.245

3.495 ± 0.202

6.239 ± 0.348

5.299 ± 0.213

6.281 ± 0.245

6.93 ± 0.206

1.929 ± 0.139

2.87 ± 0.163
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski