Amino acid dipepetide frequency for Mycobacterium phage EvilGenius

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.776AlaAla: 10.776 ± 1.02
0.476AlaCys: 0.476 ± 0.175
5.358AlaAsp: 5.358 ± 0.589
6.906AlaGlu: 6.906 ± 0.73
4.287AlaPhe: 4.287 ± 0.533
8.514AlaGly: 8.514 ± 0.802
1.727AlaHis: 1.727 ± 0.336
4.525AlaIle: 4.525 ± 0.51
4.525AlaLys: 4.525 ± 0.536
8.693AlaLeu: 8.693 ± 0.87
2.262AlaMet: 2.262 ± 0.323
2.977AlaAsn: 2.977 ± 0.433
5.12AlaPro: 5.12 ± 0.786
4.227AlaGln: 4.227 ± 0.529
6.43AlaArg: 6.43 ± 0.667
4.882AlaSer: 4.882 ± 0.617
5.656AlaThr: 5.656 ± 0.605
7.621AlaVal: 7.621 ± 0.647
1.548AlaTrp: 1.548 ± 0.294
2.262AlaTyr: 2.262 ± 0.367
0.0AlaXaa: 0.0 ± 0.0
Cys
0.476CysAla: 0.476 ± 0.168
0.119CysCys: 0.119 ± 0.111
0.774CysAsp: 0.774 ± 0.182
0.476CysGlu: 0.476 ± 0.151
0.357CysPhe: 0.357 ± 0.146
0.953CysGly: 0.953 ± 0.251
0.238CysHis: 0.238 ± 0.136
0.238CysIle: 0.238 ± 0.105
0.476CysLys: 0.476 ± 0.167
0.774CysLeu: 0.774 ± 0.215
0.298CysMet: 0.298 ± 0.14
0.476CysAsn: 0.476 ± 0.166
0.417CysPro: 0.417 ± 0.185
0.06CysGln: 0.06 ± 0.055
0.655CysArg: 0.655 ± 0.198
0.417CysSer: 0.417 ± 0.147
0.714CysThr: 0.714 ± 0.204
0.595CysVal: 0.595 ± 0.177
0.357CysTrp: 0.357 ± 0.154
0.298CysTyr: 0.298 ± 0.117
0.0CysXaa: 0.0 ± 0.0
Asp
7.085AspAla: 7.085 ± 0.685
0.655AspCys: 0.655 ± 0.206
3.751AspAsp: 3.751 ± 0.557
4.406AspGlu: 4.406 ± 0.617
3.036AspPhe: 3.036 ± 0.502
5.716AspGly: 5.716 ± 0.598
1.369AspHis: 1.369 ± 0.306
3.751AspIle: 3.751 ± 0.459
2.679AspLys: 2.679 ± 0.332
5.894AspLeu: 5.894 ± 0.655
1.846AspMet: 1.846 ± 0.309
1.131AspAsn: 1.131 ± 0.223
4.227AspPro: 4.227 ± 0.609
1.786AspGln: 1.786 ± 0.348
3.096AspArg: 3.096 ± 0.425
2.56AspSer: 2.56 ± 0.428
3.334AspThr: 3.334 ± 0.405
4.227AspVal: 4.227 ± 0.399
1.429AspTrp: 1.429 ± 0.353
2.382AspTyr: 2.382 ± 0.339
0.0AspXaa: 0.0 ± 0.0
Glu
6.668GluAla: 6.668 ± 0.699
0.298GluCys: 0.298 ± 0.148
5.239GluAsp: 5.239 ± 0.597
4.584GluGlu: 4.584 ± 0.545
2.56GluPhe: 2.56 ± 0.375
4.942GluGly: 4.942 ± 0.62
2.024GluHis: 2.024 ± 0.369
3.334GluIle: 3.334 ± 0.387
1.965GluLys: 1.965 ± 0.314
7.204GluLeu: 7.204 ± 0.631
2.024GluMet: 2.024 ± 0.352
2.143GluAsn: 2.143 ± 0.377
2.977GluPro: 2.977 ± 0.485
2.024GluGln: 2.024 ± 0.354
4.346GluArg: 4.346 ± 0.551
3.394GluSer: 3.394 ± 0.492
3.691GluThr: 3.691 ± 0.427
4.108GluVal: 4.108 ± 0.427
1.191GluTrp: 1.191 ± 0.242
2.084GluTyr: 2.084 ± 0.292
0.0GluXaa: 0.0 ± 0.0
Phe
3.453PheAla: 3.453 ± 0.448
0.357PheCys: 0.357 ± 0.16
2.858PheAsp: 2.858 ± 0.421
2.084PheGlu: 2.084 ± 0.384
0.834PhePhe: 0.834 ± 0.226
3.394PheGly: 3.394 ± 0.467
1.012PheHis: 1.012 ± 0.277
1.369PheIle: 1.369 ± 0.291
1.727PheLys: 1.727 ± 0.327
2.977PheLeu: 2.977 ± 0.489
0.476PheMet: 0.476 ± 0.147
1.727PheAsn: 1.727 ± 0.38
2.382PhePro: 2.382 ± 0.422
1.31PheGln: 1.31 ± 0.252
1.846PheArg: 1.846 ± 0.285
1.846PheSer: 1.846 ± 0.341
2.501PheThr: 2.501 ± 0.288
2.322PheVal: 2.322 ± 0.359
0.357PheTrp: 0.357 ± 0.129
0.834PheTyr: 0.834 ± 0.195
0.0PheXaa: 0.0 ± 0.0
Gly
7.383GlyAla: 7.383 ± 1.027
0.595GlyCys: 0.595 ± 0.184
5.716GlyAsp: 5.716 ± 0.69
4.823GlyGlu: 4.823 ± 0.702
3.036GlyPhe: 3.036 ± 0.461
8.276GlyGly: 8.276 ± 1.418
1.31GlyHis: 1.31 ± 0.258
3.751GlyIle: 3.751 ± 0.489
3.93GlyLys: 3.93 ± 0.412
6.549GlyLeu: 6.549 ± 0.608
1.905GlyMet: 1.905 ± 0.359
3.691GlyAsn: 3.691 ± 0.38
3.394GlyPro: 3.394 ± 0.427
2.977GlyGln: 2.977 ± 0.371
3.81GlyArg: 3.81 ± 0.405
4.525GlySer: 4.525 ± 0.685
6.073GlyThr: 6.073 ± 0.682
6.609GlyVal: 6.609 ± 0.646
1.905GlyTrp: 1.905 ± 0.353
2.501GlyTyr: 2.501 ± 0.365
0.0GlyXaa: 0.0 ± 0.0
His
1.548HisAla: 1.548 ± 0.292
0.357HisCys: 0.357 ± 0.137
1.25HisAsp: 1.25 ± 0.307
0.953HisGlu: 0.953 ± 0.201
0.417HisPhe: 0.417 ± 0.157
1.965HisGly: 1.965 ± 0.43
0.417HisHis: 0.417 ± 0.147
1.131HisIle: 1.131 ± 0.282
0.774HisLys: 0.774 ± 0.193
1.667HisLeu: 1.667 ± 0.378
0.298HisMet: 0.298 ± 0.148
0.714HisAsn: 0.714 ± 0.257
1.131HisPro: 1.131 ± 0.217
0.893HisGln: 0.893 ± 0.26
1.608HisArg: 1.608 ± 0.374
0.774HisSer: 0.774 ± 0.258
1.191HisThr: 1.191 ± 0.247
1.369HisVal: 1.369 ± 0.286
0.536HisTrp: 0.536 ± 0.167
0.655HisTyr: 0.655 ± 0.241
0.0HisXaa: 0.0 ± 0.0
Ile
4.823IleAla: 4.823 ± 0.584
0.893IleCys: 0.893 ± 0.186
3.632IleAsp: 3.632 ± 0.473
4.584IleGlu: 4.584 ± 0.514
1.548IlePhe: 1.548 ± 0.266
5.12IleGly: 5.12 ± 0.579
1.012IleHis: 1.012 ± 0.193
2.024IleIle: 2.024 ± 0.302
2.62IleLys: 2.62 ± 0.375
3.691IleLeu: 3.691 ± 0.428
0.357IleMet: 0.357 ± 0.131
2.262IleAsn: 2.262 ± 0.363
3.453IlePro: 3.453 ± 0.447
1.429IleGln: 1.429 ± 0.277
2.858IleArg: 2.858 ± 0.384
2.858IleSer: 2.858 ± 0.388
2.739IleThr: 2.739 ± 0.347
2.382IleVal: 2.382 ± 0.344
0.417IleTrp: 0.417 ± 0.137
1.191IleTyr: 1.191 ± 0.228
0.0IleXaa: 0.0 ± 0.0
Lys
4.942LysAla: 4.942 ± 0.53
0.238LysCys: 0.238 ± 0.155
2.441LysAsp: 2.441 ± 0.425
2.679LysGlu: 2.679 ± 0.337
1.191LysPhe: 1.191 ± 0.268
3.87LysGly: 3.87 ± 0.515
0.714LysHis: 0.714 ± 0.213
2.739LysIle: 2.739 ± 0.429
3.275LysLys: 3.275 ± 0.53
3.93LysLeu: 3.93 ± 0.402
0.953LysMet: 0.953 ± 0.255
1.667LysAsn: 1.667 ± 0.294
2.56LysPro: 2.56 ± 0.425
1.667LysGln: 1.667 ± 0.344
3.275LysArg: 3.275 ± 0.458
2.679LysSer: 2.679 ± 0.356
2.739LysThr: 2.739 ± 0.373
3.93LysVal: 3.93 ± 0.396
0.714LysTrp: 0.714 ± 0.209
1.667LysTyr: 1.667 ± 0.294
0.0LysXaa: 0.0 ± 0.0
Leu
9.05LeuAla: 9.05 ± 0.747
0.655LeuCys: 0.655 ± 0.207
5.477LeuAsp: 5.477 ± 0.574
5.418LeuGlu: 5.418 ± 0.587
2.858LeuPhe: 2.858 ± 0.393
5.477LeuGly: 5.477 ± 0.708
1.488LeuHis: 1.488 ± 0.303
4.168LeuIle: 4.168 ± 0.464
4.168LeuLys: 4.168 ± 0.578
5.597LeuLeu: 5.597 ± 0.658
2.62LeuMet: 2.62 ± 0.355
2.203LeuAsn: 2.203 ± 0.363
4.882LeuPro: 4.882 ± 0.534
2.798LeuGln: 2.798 ± 0.51
5.894LeuArg: 5.894 ± 0.66
6.013LeuSer: 6.013 ± 0.563
6.132LeuThr: 6.132 ± 0.677
4.644LeuVal: 4.644 ± 0.601
1.488LeuTrp: 1.488 ± 0.257
2.203LeuTyr: 2.203 ± 0.448
0.0LeuXaa: 0.0 ± 0.0
Met
2.917MetAla: 2.917 ± 0.347
0.119MetCys: 0.119 ± 0.072
1.25MetAsp: 1.25 ± 0.271
0.774MetGlu: 0.774 ± 0.162
0.834MetPhe: 0.834 ± 0.234
1.727MetGly: 1.727 ± 0.318
0.595MetHis: 0.595 ± 0.208
1.191MetIle: 1.191 ± 0.278
1.488MetLys: 1.488 ± 0.25
1.786MetLeu: 1.786 ± 0.4
0.774MetMet: 0.774 ± 0.233
0.774MetAsn: 0.774 ± 0.233
1.31MetPro: 1.31 ± 0.291
0.953MetGln: 0.953 ± 0.235
1.31MetArg: 1.31 ± 0.262
2.382MetSer: 2.382 ± 0.321
1.786MetThr: 1.786 ± 0.277
1.191MetVal: 1.191 ± 0.342
0.179MetTrp: 0.179 ± 0.087
0.655MetTyr: 0.655 ± 0.214
0.0MetXaa: 0.0 ± 0.0
Asn
2.858AsnAla: 2.858 ± 0.308
0.476AsnCys: 0.476 ± 0.173
1.846AsnAsp: 1.846 ± 0.308
2.084AsnGlu: 2.084 ± 0.385
0.714AsnPhe: 0.714 ± 0.19
3.691AsnGly: 3.691 ± 0.474
0.893AsnHis: 0.893 ± 0.214
1.548AsnIle: 1.548 ± 0.259
1.488AsnLys: 1.488 ± 0.329
3.215AsnLeu: 3.215 ± 0.399
0.953AsnMet: 0.953 ± 0.224
0.655AsnAsn: 0.655 ± 0.202
2.501AsnPro: 2.501 ± 0.39
0.953AsnGln: 0.953 ± 0.263
1.846AsnArg: 1.846 ± 0.414
1.31AsnSer: 1.31 ± 0.243
1.488AsnThr: 1.488 ± 0.3
2.203AsnVal: 2.203 ± 0.375
1.012AsnTrp: 1.012 ± 0.241
0.893AsnTyr: 0.893 ± 0.203
0.0AsnXaa: 0.0 ± 0.0
Pro
5.358ProAla: 5.358 ± 0.692
0.298ProCys: 0.298 ± 0.144
4.168ProAsp: 4.168 ± 0.556
4.942ProGlu: 4.942 ± 0.537
1.965ProPhe: 1.965 ± 0.344
4.406ProGly: 4.406 ± 0.58
1.012ProHis: 1.012 ± 0.267
2.441ProIle: 2.441 ± 0.337
2.441ProLys: 2.441 ± 0.409
3.87ProLeu: 3.87 ± 0.51
1.191ProMet: 1.191 ± 0.257
1.846ProAsn: 1.846 ± 0.329
2.501ProPro: 2.501 ± 0.422
1.608ProGln: 1.608 ± 0.364
3.81ProArg: 3.81 ± 0.523
3.036ProSer: 3.036 ± 0.509
3.87ProThr: 3.87 ± 0.504
3.751ProVal: 3.751 ± 0.488
1.012ProTrp: 1.012 ± 0.335
1.25ProTyr: 1.25 ± 0.238
0.0ProXaa: 0.0 ± 0.0
Gln
4.227GlnAla: 4.227 ± 0.584
0.179GlnCys: 0.179 ± 0.093
1.488GlnAsp: 1.488 ± 0.287
1.667GlnGlu: 1.667 ± 0.345
1.31GlnPhe: 1.31 ± 0.248
2.501GlnGly: 2.501 ± 0.397
0.655GlnHis: 0.655 ± 0.203
2.62GlnIle: 2.62 ± 0.391
1.548GlnLys: 1.548 ± 0.402
3.453GlnLeu: 3.453 ± 0.659
1.012GlnMet: 1.012 ± 0.24
0.595GlnAsn: 0.595 ± 0.195
1.191GlnPro: 1.191 ± 0.277
1.846GlnGln: 1.846 ± 0.373
2.322GlnArg: 2.322 ± 0.407
1.667GlnSer: 1.667 ± 0.298
2.441GlnThr: 2.441 ± 0.405
2.56GlnVal: 2.56 ± 0.367
0.714GlnTrp: 0.714 ± 0.186
0.953GlnTyr: 0.953 ± 0.237
0.0GlnXaa: 0.0 ± 0.0
Arg
4.406ArgAla: 4.406 ± 0.606
0.893ArgCys: 0.893 ± 0.264
4.287ArgAsp: 4.287 ± 0.502
5.001ArgGlu: 5.001 ± 0.625
2.382ArgPhe: 2.382 ± 0.364
4.108ArgGly: 4.108 ± 0.49
1.369ArgHis: 1.369 ± 0.267
3.691ArgIle: 3.691 ± 0.57
3.572ArgLys: 3.572 ± 0.441
4.704ArgLeu: 4.704 ± 0.548
1.608ArgMet: 1.608 ± 0.29
2.441ArgAsn: 2.441 ± 0.362
3.036ArgPro: 3.036 ± 0.344
1.846ArgGln: 1.846 ± 0.313
5.18ArgArg: 5.18 ± 0.649
2.679ArgSer: 2.679 ± 0.368
3.572ArgThr: 3.572 ± 0.423
4.406ArgVal: 4.406 ± 0.584
1.369ArgTrp: 1.369 ± 0.285
1.846ArgTyr: 1.846 ± 0.37
0.0ArgXaa: 0.0 ± 0.0
Ser
5.001SerAla: 5.001 ± 0.548
0.595SerCys: 0.595 ± 0.191
2.917SerAsp: 2.917 ± 0.377
4.168SerGlu: 4.168 ± 0.348
2.084SerPhe: 2.084 ± 0.344
4.763SerGly: 4.763 ± 0.655
0.655SerHis: 0.655 ± 0.212
2.143SerIle: 2.143 ± 0.32
2.858SerLys: 2.858 ± 0.425
4.287SerLeu: 4.287 ± 0.676
1.429SerMet: 1.429 ± 0.269
1.429SerAsn: 1.429 ± 0.325
3.691SerPro: 3.691 ± 0.437
1.905SerGln: 1.905 ± 0.339
3.513SerArg: 3.513 ± 0.362
2.382SerSer: 2.382 ± 0.45
2.977SerThr: 2.977 ± 0.358
3.453SerVal: 3.453 ± 0.525
1.31SerTrp: 1.31 ± 0.268
1.429SerTyr: 1.429 ± 0.292
0.0SerXaa: 0.0 ± 0.0
Thr
6.311ThrAla: 6.311 ± 0.708
0.714ThrCys: 0.714 ± 0.225
3.632ThrAsp: 3.632 ± 0.603
3.513ThrGlu: 3.513 ± 0.412
2.262ThrPhe: 2.262 ± 0.365
5.12ThrGly: 5.12 ± 0.723
0.953ThrHis: 0.953 ± 0.28
3.453ThrIle: 3.453 ± 0.365
2.858ThrLys: 2.858 ± 0.512
5.299ThrLeu: 5.299 ± 0.606
1.31ThrMet: 1.31 ± 0.252
1.727ThrAsn: 1.727 ± 0.351
4.584ThrPro: 4.584 ± 0.599
2.322ThrGln: 2.322 ± 0.376
3.215ThrArg: 3.215 ± 0.43
2.858ThrSer: 2.858 ± 0.375
3.81ThrThr: 3.81 ± 0.582
5.477ThrVal: 5.477 ± 0.611
1.25ThrTrp: 1.25 ± 0.251
2.143ThrTyr: 2.143 ± 0.317
0.0ThrXaa: 0.0 ± 0.0
Val
6.668ValAla: 6.668 ± 0.812
0.595ValCys: 0.595 ± 0.182
5.12ValAsp: 5.12 ± 0.507
4.227ValGlu: 4.227 ± 0.557
2.501ValPhe: 2.501 ± 0.533
4.763ValGly: 4.763 ± 0.541
1.31ValHis: 1.31 ± 0.266
3.334ValIle: 3.334 ± 0.506
3.93ValLys: 3.93 ± 0.564
5.299ValLeu: 5.299 ± 0.63
1.25ValMet: 1.25 ± 0.229
2.739ValAsn: 2.739 ± 0.482
3.096ValPro: 3.096 ± 0.464
2.501ValGln: 2.501 ± 0.426
4.168ValArg: 4.168 ± 0.509
4.346ValSer: 4.346 ± 0.42
4.882ValThr: 4.882 ± 0.417
5.001ValVal: 5.001 ± 0.521
1.429ValTrp: 1.429 ± 0.337
2.143ValTyr: 2.143 ± 0.396
0.0ValXaa: 0.0 ± 0.0
Trp
1.965TrpAla: 1.965 ± 0.384
0.357TrpCys: 0.357 ± 0.133
1.012TrpAsp: 1.012 ± 0.228
1.25TrpGlu: 1.25 ± 0.255
0.655TrpPhe: 0.655 ± 0.204
1.369TrpGly: 1.369 ± 0.321
0.595TrpHis: 0.595 ± 0.211
0.834TrpIle: 0.834 ± 0.196
0.834TrpLys: 0.834 ± 0.227
1.667TrpLeu: 1.667 ± 0.265
0.536TrpMet: 0.536 ± 0.176
0.774TrpAsn: 0.774 ± 0.191
0.953TrpPro: 0.953 ± 0.285
0.893TrpGln: 0.893 ± 0.193
1.012TrpArg: 1.012 ± 0.195
1.072TrpSer: 1.072 ± 0.263
1.31TrpThr: 1.31 ± 0.306
1.131TrpVal: 1.131 ± 0.2
0.595TrpTrp: 0.595 ± 0.211
0.476TrpTyr: 0.476 ± 0.176
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.858TyrAla: 2.858 ± 0.34
0.298TyrCys: 0.298 ± 0.164
2.203TyrAsp: 2.203 ± 0.35
2.262TyrGlu: 2.262 ± 0.37
0.834TyrPhe: 0.834 ± 0.206
2.084TyrGly: 2.084 ± 0.315
0.298TyrHis: 0.298 ± 0.12
1.608TyrIle: 1.608 ± 0.281
0.714TyrLys: 0.714 ± 0.18
2.679TyrLeu: 2.679 ± 0.449
0.834TyrMet: 0.834 ± 0.224
0.655TyrAsn: 0.655 ± 0.194
1.369TyrPro: 1.369 ± 0.243
0.953TyrGln: 0.953 ± 0.208
2.203TyrArg: 2.203 ± 0.372
1.31TyrSer: 1.31 ± 0.257
1.905TyrThr: 1.905 ± 0.296
2.322TyrVal: 2.322 ± 0.387
0.476TyrTrp: 0.476 ± 0.205
0.834TyrTyr: 0.834 ± 0.219
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 93 proteins (16797 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski