Gordonia phage Dorito

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S9UAI3|A0A3S9UAI3_9CAUD Integrase OS=Gordonia phage Dorito OX=2499023 GN=32 PE=3 SV=1
MM1 pKa = 7.25MVTPSNVAEE10 pKa = 3.98FHH12 pKa = 7.05DD13 pKa = 4.5AGEE16 pKa = 4.6GTFWWHH22 pKa = 7.15DD23 pKa = 3.55PAADD27 pKa = 4.11EE28 pKa = 4.73YY29 pKa = 11.38LITDD33 pKa = 4.49TFTPPVDD40 pKa = 3.19GALYY44 pKa = 10.63LMPWDD49 pKa = 4.55GAWFAQWDD57 pKa = 4.45GDD59 pKa = 3.46WEE61 pKa = 4.26AAVEE65 pKa = 3.96QLNSIIEE72 pKa = 4.2RR73 pKa = 11.84RR74 pKa = 11.84QEE76 pKa = 3.84TAA78 pKa = 3.28

Molecular weight: 8.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S9UAL2|A0A3S9UAL2_9CAUD Uncharacterized protein OS=Gordonia phage Dorito OX=2499023 GN=70 PE=4 SV=1
MM1 pKa = 7.37TLRR4 pKa = 11.84RR5 pKa = 11.84CTRR8 pKa = 11.84PGCEE12 pKa = 3.41KK13 pKa = 10.45RR14 pKa = 11.84PRR16 pKa = 11.84SRR18 pKa = 11.84GLCHH22 pKa = 5.62THH24 pKa = 6.78YY25 pKa = 9.14MDD27 pKa = 6.13HH28 pKa = 6.62ITRR31 pKa = 11.84QKK33 pKa = 11.18AYY35 pKa = 10.13GRR37 pKa = 11.84WEE39 pKa = 4.01SSLVDD44 pKa = 3.54AEE46 pKa = 4.16PTRR49 pKa = 11.84QHH51 pKa = 5.98VEE53 pKa = 3.61RR54 pKa = 11.84LRR56 pKa = 11.84AAGVGTRR63 pKa = 11.84KK64 pKa = 9.59ISEE67 pKa = 4.23TAGVTRR73 pKa = 11.84SVLQSLIHH81 pKa = 5.91GKK83 pKa = 7.21TRR85 pKa = 11.84NGHH88 pKa = 6.0RR89 pKa = 11.84EE90 pKa = 4.03PPSKK94 pKa = 9.88TIAKK98 pKa = 9.77RR99 pKa = 11.84IADD102 pKa = 4.46SILAVPVPAQPLRR115 pKa = 11.84NGDD118 pKa = 3.62VPALGTRR125 pKa = 11.84RR126 pKa = 11.84RR127 pKa = 11.84LQALIAHH134 pKa = 7.62GYY136 pKa = 7.86SQAEE140 pKa = 3.93INRR143 pKa = 11.84RR144 pKa = 11.84FGLAADD150 pKa = 4.22NLTEE154 pKa = 5.09LIRR157 pKa = 11.84GEE159 pKa = 4.17RR160 pKa = 11.84NYY162 pKa = 10.52VDD164 pKa = 3.76PKK166 pKa = 7.62TARR169 pKa = 11.84RR170 pKa = 11.84VAALFRR176 pKa = 11.84EE177 pKa = 4.57LQLTPGPCTRR187 pKa = 11.84ARR189 pKa = 11.84NRR191 pKa = 11.84GKK193 pKa = 10.87ANGWPLPLDD202 pKa = 3.41WDD204 pKa = 4.19EE205 pKa = 5.62DD206 pKa = 4.73HH207 pKa = 7.6IDD209 pKa = 4.15DD210 pKa = 5.48PNNQAEE216 pKa = 4.2RR217 pKa = 11.84SHH219 pKa = 5.91VRR221 pKa = 11.84SIVRR225 pKa = 11.84DD226 pKa = 3.49PEE228 pKa = 4.53RR229 pKa = 11.84IEE231 pKa = 3.58WRR233 pKa = 11.84KK234 pKa = 9.75QKK236 pKa = 10.48VAEE239 pKa = 4.18LTALGYY245 pKa = 9.82SAEE248 pKa = 4.89DD249 pKa = 2.74IAARR253 pKa = 11.84LRR255 pKa = 11.84CTARR259 pKa = 11.84TVQRR263 pKa = 11.84DD264 pKa = 3.45RR265 pKa = 11.84SALGIVEE272 pKa = 4.47RR273 pKa = 11.84EE274 pKa = 4.06QVASS278 pKa = 3.61

Molecular weight: 31.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

0

75

15130

37

2138

201.7

22.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.89 ± 0.654

0.846 ± 0.171

6.907 ± 0.421

5.935 ± 0.264

2.406 ± 0.136

8.057 ± 0.501

2.254 ± 0.239

4.362 ± 0.189

3.206 ± 0.24

8.044 ± 0.182

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.247 ± 0.136

2.829 ± 0.221

5.506 ± 0.268

3.807 ± 0.327

7.905 ± 0.498

5.274 ± 0.174

7.33 ± 0.309

7.145 ± 0.222

1.956 ± 0.183

2.095 ± 0.135
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski