Amino acid dipepetide frequency for Gordonia phage Dorito

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.458AlaAla: 16.458 ± 1.521
0.859AlaCys: 0.859 ± 0.225
8.659AlaAsp: 8.659 ± 0.662
8.196AlaGlu: 8.196 ± 0.726
2.247AlaPhe: 2.247 ± 0.497
9.254AlaGly: 9.254 ± 0.772
2.446AlaHis: 2.446 ± 0.523
5.486AlaIle: 5.486 ± 0.511
4.429AlaLys: 4.429 ± 0.535
8.989AlaLeu: 8.989 ± 0.744
3.9AlaMet: 3.9 ± 0.615
3.503AlaAsn: 3.503 ± 0.522
4.957AlaPro: 4.957 ± 0.774
4.891AlaGln: 4.891 ± 0.881
8.394AlaArg: 8.394 ± 0.752
6.015AlaSer: 6.015 ± 0.787
8.262AlaThr: 8.262 ± 0.865
7.998AlaVal: 7.998 ± 0.935
1.785AlaTrp: 1.785 ± 0.349
2.181AlaTyr: 2.181 ± 0.384
0.0AlaXaa: 0.0 ± 0.0
Cys
0.661CysAla: 0.661 ± 0.182
0.33CysCys: 0.33 ± 0.177
0.595CysAsp: 0.595 ± 0.233
0.595CysGlu: 0.595 ± 0.195
0.132CysPhe: 0.132 ± 0.101
1.058CysGly: 1.058 ± 0.3
0.33CysHis: 0.33 ± 0.164
0.198CysIle: 0.198 ± 0.113
0.132CysLys: 0.132 ± 0.087
0.33CysLeu: 0.33 ± 0.149
0.132CysMet: 0.132 ± 0.091
0.33CysAsn: 0.33 ± 0.14
0.33CysPro: 0.33 ± 0.144
0.264CysGln: 0.264 ± 0.151
0.925CysArg: 0.925 ± 0.263
0.397CysSer: 0.397 ± 0.229
0.727CysThr: 0.727 ± 0.285
0.463CysVal: 0.463 ± 0.197
0.132CysTrp: 0.132 ± 0.112
0.397CysTyr: 0.397 ± 0.177
0.0CysXaa: 0.0 ± 0.0
Asp
6.94AspAla: 6.94 ± 0.638
0.397AspCys: 0.397 ± 0.168
6.015AspAsp: 6.015 ± 0.876
5.156AspGlu: 5.156 ± 0.718
1.52AspPhe: 1.52 ± 0.261
4.891AspGly: 4.891 ± 0.661
2.38AspHis: 2.38 ± 0.518
2.842AspIle: 2.842 ± 0.441
1.719AspLys: 1.719 ± 0.262
7.073AspLeu: 7.073 ± 0.678
1.454AspMet: 1.454 ± 0.313
1.785AspAsn: 1.785 ± 0.338
5.288AspPro: 5.288 ± 0.546
2.313AspGln: 2.313 ± 0.389
4.627AspArg: 4.627 ± 0.757
2.512AspSer: 2.512 ± 0.428
3.966AspThr: 3.966 ± 0.581
5.288AspVal: 5.288 ± 0.527
1.454AspTrp: 1.454 ± 0.29
1.454AspTyr: 1.454 ± 0.256
0.0AspXaa: 0.0 ± 0.0
Glu
7.139GluAla: 7.139 ± 0.582
0.727GluCys: 0.727 ± 0.263
2.842GluAsp: 2.842 ± 0.397
3.173GluGlu: 3.173 ± 0.592
1.983GluPhe: 1.983 ± 0.351
3.173GluGly: 3.173 ± 0.444
1.388GluHis: 1.388 ± 0.379
2.644GluIle: 2.644 ± 0.458
1.983GluLys: 1.983 ± 0.358
5.883GluLeu: 5.883 ± 0.535
1.454GluMet: 1.454 ± 0.378
1.124GluAsn: 1.124 ± 0.345
2.776GluPro: 2.776 ± 0.456
3.371GluGln: 3.371 ± 0.446
5.288GluArg: 5.288 ± 0.71
3.437GluSer: 3.437 ± 0.536
3.503GluThr: 3.503 ± 0.44
4.825GluVal: 4.825 ± 0.714
1.058GluTrp: 1.058 ± 0.24
1.586GluTyr: 1.586 ± 0.329
0.0GluXaa: 0.0 ± 0.0
Phe
2.842PheAla: 2.842 ± 0.443
0.0PheCys: 0.0 ± 0.0
2.247PheAsp: 2.247 ± 0.276
1.586PheGlu: 1.586 ± 0.279
0.661PhePhe: 0.661 ± 0.208
2.644PheGly: 2.644 ± 0.457
0.595PheHis: 0.595 ± 0.22
0.661PheIle: 0.661 ± 0.22
0.397PheLys: 0.397 ± 0.181
1.124PheLeu: 1.124 ± 0.3
0.463PheMet: 0.463 ± 0.166
0.595PheAsn: 0.595 ± 0.226
1.256PhePro: 1.256 ± 0.23
0.727PheGln: 0.727 ± 0.254
1.851PheArg: 1.851 ± 0.413
1.388PheSer: 1.388 ± 0.244
1.917PheThr: 1.917 ± 0.347
1.983PheVal: 1.983 ± 0.435
0.793PheTrp: 0.793 ± 0.226
0.33PheTyr: 0.33 ± 0.166
0.0PheXaa: 0.0 ± 0.0
Gly
8.262GlyAla: 8.262 ± 0.916
0.397GlyCys: 0.397 ± 0.154
5.751GlyAsp: 5.751 ± 0.698
4.164GlyGlu: 4.164 ± 0.356
2.115GlyPhe: 2.115 ± 0.333
7.601GlyGly: 7.601 ± 1.15
1.917GlyHis: 1.917 ± 0.432
3.569GlyIle: 3.569 ± 0.47
3.107GlyLys: 3.107 ± 0.505
5.883GlyLeu: 5.883 ± 0.464
2.247GlyMet: 2.247 ± 0.57
2.842GlyAsn: 2.842 ± 0.471
4.032GlyPro: 4.032 ± 0.533
2.71GlyGln: 2.71 ± 0.491
6.081GlyArg: 6.081 ± 0.589
4.362GlySer: 4.362 ± 0.693
5.883GlyThr: 5.883 ± 0.775
6.544GlyVal: 6.544 ± 0.766
1.322GlyTrp: 1.322 ± 0.329
1.785GlyTyr: 1.785 ± 0.29
0.0GlyXaa: 0.0 ± 0.0
His
1.917HisAla: 1.917 ± 0.383
0.264HisCys: 0.264 ± 0.143
1.19HisAsp: 1.19 ± 0.309
1.256HisGlu: 1.256 ± 0.317
0.264HisPhe: 0.264 ± 0.146
1.983HisGly: 1.983 ± 0.373
0.529HisHis: 0.529 ± 0.219
1.19HisIle: 1.19 ± 0.372
0.529HisLys: 0.529 ± 0.191
2.181HisLeu: 2.181 ± 0.44
0.397HisMet: 0.397 ± 0.147
0.595HisAsn: 0.595 ± 0.242
1.851HisPro: 1.851 ± 0.472
1.322HisGln: 1.322 ± 0.302
1.785HisArg: 1.785 ± 0.359
0.595HisSer: 0.595 ± 0.2
1.917HisThr: 1.917 ± 0.384
1.586HisVal: 1.586 ± 0.319
0.529HisTrp: 0.529 ± 0.162
0.661HisTyr: 0.661 ± 0.221
0.0HisXaa: 0.0 ± 0.0
Ile
6.345IleAla: 6.345 ± 0.752
0.198IleCys: 0.198 ± 0.109
4.032IleAsp: 4.032 ± 0.506
2.776IleGlu: 2.776 ± 0.447
0.661IlePhe: 0.661 ± 0.227
3.503IleGly: 3.503 ± 0.595
0.925IleHis: 0.925 ± 0.294
1.256IleIle: 1.256 ± 0.257
0.991IleLys: 0.991 ± 0.264
2.38IleLeu: 2.38 ± 0.338
0.529IleMet: 0.529 ± 0.156
1.785IleAsn: 1.785 ± 0.325
2.71IlePro: 2.71 ± 0.445
1.652IleGln: 1.652 ± 0.3
3.437IleArg: 3.437 ± 0.5
2.181IleSer: 2.181 ± 0.346
3.239IleThr: 3.239 ± 0.584
3.107IleVal: 3.107 ± 0.48
0.595IleTrp: 0.595 ± 0.205
1.322IleTyr: 1.322 ± 0.37
0.0IleXaa: 0.0 ± 0.0
Lys
4.296LysAla: 4.296 ± 0.603
0.066LysCys: 0.066 ± 0.068
1.256LysAsp: 1.256 ± 0.275
1.719LysGlu: 1.719 ± 0.328
0.727LysPhe: 0.727 ± 0.234
2.313LysGly: 2.313 ± 0.424
0.859LysHis: 0.859 ± 0.221
1.388LysIle: 1.388 ± 0.261
1.058LysLys: 1.058 ± 0.33
3.239LysLeu: 3.239 ± 0.451
0.33LysMet: 0.33 ± 0.136
0.793LysAsn: 0.793 ± 0.166
2.313LysPro: 2.313 ± 0.447
1.322LysGln: 1.322 ± 0.405
2.38LysArg: 2.38 ± 0.487
1.983LysSer: 1.983 ± 0.428
2.247LysThr: 2.247 ± 0.434
1.983LysVal: 1.983 ± 0.337
0.793LysTrp: 0.793 ± 0.275
0.991LysTyr: 0.991 ± 0.208
0.0LysXaa: 0.0 ± 0.0
Leu
9.981LeuAla: 9.981 ± 0.879
0.595LeuCys: 0.595 ± 0.211
6.478LeuAsp: 6.478 ± 0.713
5.156LeuGlu: 5.156 ± 0.677
2.181LeuPhe: 2.181 ± 0.525
6.081LeuGly: 6.081 ± 0.65
1.256LeuHis: 1.256 ± 0.282
3.173LeuIle: 3.173 ± 0.393
2.247LeuLys: 2.247 ± 0.378
5.684LeuLeu: 5.684 ± 0.587
1.983LeuMet: 1.983 ± 0.354
2.181LeuAsn: 2.181 ± 0.439
4.296LeuPro: 4.296 ± 0.69
2.908LeuGln: 2.908 ± 0.411
6.478LeuArg: 6.478 ± 0.593
4.627LeuSer: 4.627 ± 0.452
7.073LeuThr: 7.073 ± 0.653
5.817LeuVal: 5.817 ± 0.779
1.124LeuTrp: 1.124 ± 0.227
1.124LeuTyr: 1.124 ± 0.256
0.0LeuXaa: 0.0 ± 0.0
Met
2.974MetAla: 2.974 ± 0.587
0.198MetCys: 0.198 ± 0.113
0.859MetAsp: 0.859 ± 0.255
0.397MetGlu: 0.397 ± 0.161
0.33MetPhe: 0.33 ± 0.143
1.52MetGly: 1.52 ± 0.352
1.124MetHis: 1.124 ± 0.293
0.463MetIle: 0.463 ± 0.165
0.793MetLys: 0.793 ± 0.207
1.917MetLeu: 1.917 ± 0.348
0.529MetMet: 0.529 ± 0.206
0.925MetAsn: 0.925 ± 0.268
1.785MetPro: 1.785 ± 0.281
0.661MetGln: 0.661 ± 0.239
2.313MetArg: 2.313 ± 0.352
1.322MetSer: 1.322 ± 0.278
2.578MetThr: 2.578 ± 0.379
1.256MetVal: 1.256 ± 0.247
0.463MetTrp: 0.463 ± 0.227
0.066MetTyr: 0.066 ± 0.064
0.0MetXaa: 0.0 ± 0.0
Asn
2.908AsnAla: 2.908 ± 0.668
0.132AsnCys: 0.132 ± 0.093
1.983AsnAsp: 1.983 ± 0.389
1.652AsnGlu: 1.652 ± 0.281
0.529AsnPhe: 0.529 ± 0.167
2.776AsnGly: 2.776 ± 0.482
0.793AsnHis: 0.793 ± 0.203
1.322AsnIle: 1.322 ± 0.237
1.256AsnLys: 1.256 ± 0.344
1.851AsnLeu: 1.851 ± 0.313
0.529AsnMet: 0.529 ± 0.164
0.925AsnAsn: 0.925 ± 0.277
2.049AsnPro: 2.049 ± 0.357
1.19AsnGln: 1.19 ± 0.276
2.644AsnArg: 2.644 ± 0.481
1.785AsnSer: 1.785 ± 0.255
1.917AsnThr: 1.917 ± 0.415
1.058AsnVal: 1.058 ± 0.26
0.463AsnTrp: 0.463 ± 0.155
0.529AsnTyr: 0.529 ± 0.184
0.0AsnXaa: 0.0 ± 0.0
Pro
6.94ProAla: 6.94 ± 0.685
0.661ProCys: 0.661 ± 0.216
4.495ProAsp: 4.495 ± 0.876
3.503ProGlu: 3.503 ± 0.472
1.124ProPhe: 1.124 ± 0.286
5.288ProGly: 5.288 ± 0.718
0.793ProHis: 0.793 ± 0.281
1.983ProIle: 1.983 ± 0.303
1.52ProLys: 1.52 ± 0.255
3.768ProLeu: 3.768 ± 0.428
1.256ProMet: 1.256 ± 0.267
1.983ProAsn: 1.983 ± 0.291
2.644ProPro: 2.644 ± 0.471
1.058ProGln: 1.058 ± 0.25
3.768ProArg: 3.768 ± 0.626
3.173ProSer: 3.173 ± 0.382
4.693ProThr: 4.693 ± 0.569
4.164ProVal: 4.164 ± 0.568
1.388ProTrp: 1.388 ± 0.301
0.859ProTyr: 0.859 ± 0.212
0.0ProXaa: 0.0 ± 0.0
Gln
4.693GlnAla: 4.693 ± 0.677
0.397GlnCys: 0.397 ± 0.174
1.785GlnAsp: 1.785 ± 0.277
1.586GlnGlu: 1.586 ± 0.324
0.991GlnPhe: 0.991 ± 0.241
2.049GlnGly: 2.049 ± 0.542
0.859GlnHis: 0.859 ± 0.291
2.38GlnIle: 2.38 ± 0.483
1.652GlnLys: 1.652 ± 0.352
4.561GlnLeu: 4.561 ± 0.483
1.124GlnMet: 1.124 ± 0.216
0.661GlnAsn: 0.661 ± 0.239
1.388GlnPro: 1.388 ± 0.244
1.652GlnGln: 1.652 ± 0.367
3.635GlnArg: 3.635 ± 0.547
1.851GlnSer: 1.851 ± 0.348
2.446GlnThr: 2.446 ± 0.485
2.974GlnVal: 2.974 ± 0.502
0.859GlnTrp: 0.859 ± 0.207
0.529GlnTyr: 0.529 ± 0.178
0.0GlnXaa: 0.0 ± 0.0
Arg
7.733ArgAla: 7.733 ± 0.771
0.925ArgCys: 0.925 ± 0.272
4.429ArgAsp: 4.429 ± 0.565
4.23ArgGlu: 4.23 ± 0.522
2.512ArgPhe: 2.512 ± 0.391
5.42ArgGly: 5.42 ± 0.638
1.454ArgHis: 1.454 ± 0.308
3.768ArgIle: 3.768 ± 0.602
2.908ArgLys: 2.908 ± 0.46
7.535ArgLeu: 7.535 ± 0.568
1.983ArgMet: 1.983 ± 0.332
2.776ArgAsn: 2.776 ± 0.474
4.23ArgPro: 4.23 ± 0.615
3.503ArgGln: 3.503 ± 0.506
7.006ArgArg: 7.006 ± 0.95
4.23ArgSer: 4.23 ± 0.545
5.09ArgThr: 5.09 ± 0.523
4.825ArgVal: 4.825 ± 0.429
2.644ArgTrp: 2.644 ± 0.481
1.851ArgTyr: 1.851 ± 0.321
0.0ArgXaa: 0.0 ± 0.0
Ser
6.544SerAla: 6.544 ± 0.638
0.264SerCys: 0.264 ± 0.132
3.437SerAsp: 3.437 ± 0.38
2.974SerGlu: 2.974 ± 0.367
1.322SerPhe: 1.322 ± 0.329
5.222SerGly: 5.222 ± 0.74
0.991SerHis: 0.991 ± 0.246
2.71SerIle: 2.71 ± 0.384
1.586SerLys: 1.586 ± 0.308
4.032SerLeu: 4.032 ± 0.483
1.256SerMet: 1.256 ± 0.305
1.322SerAsn: 1.322 ± 0.321
2.512SerPro: 2.512 ± 0.373
2.38SerGln: 2.38 ± 0.515
3.437SerArg: 3.437 ± 0.456
2.908SerSer: 2.908 ± 0.456
4.032SerThr: 4.032 ± 0.539
3.569SerVal: 3.569 ± 0.429
0.991SerTrp: 0.991 ± 0.277
1.256SerTyr: 1.256 ± 0.377
0.0SerXaa: 0.0 ± 0.0
Thr
9.254ThrAla: 9.254 ± 0.976
0.727ThrCys: 0.727 ± 0.218
4.957ThrAsp: 4.957 ± 0.661
3.702ThrGlu: 3.702 ± 0.462
1.586ThrPhe: 1.586 ± 0.33
7.006ThrGly: 7.006 ± 0.721
1.058ThrHis: 1.058 ± 0.229
4.098ThrIle: 4.098 ± 0.552
2.247ThrLys: 2.247 ± 0.357
5.751ThrLeu: 5.751 ± 0.583
0.859ThrMet: 0.859 ± 0.201
1.256ThrAsn: 1.256 ± 0.224
5.09ThrPro: 5.09 ± 0.731
2.049ThrGln: 2.049 ± 0.449
5.618ThrArg: 5.618 ± 0.818
3.107ThrSer: 3.107 ± 0.47
5.486ThrThr: 5.486 ± 0.725
6.015ThrVal: 6.015 ± 0.637
1.719ThrTrp: 1.719 ± 0.464
1.719ThrTyr: 1.719 ± 0.34
0.0ThrXaa: 0.0 ± 0.0
Val
8.461ValAla: 8.461 ± 0.822
0.859ValCys: 0.859 ± 0.234
5.751ValAsp: 5.751 ± 0.594
5.354ValGlu: 5.354 ± 0.548
1.917ValPhe: 1.917 ± 0.359
5.354ValGly: 5.354 ± 0.598
1.52ValHis: 1.52 ± 0.31
3.239ValIle: 3.239 ± 0.419
2.446ValLys: 2.446 ± 0.371
4.891ValLeu: 4.891 ± 0.609
0.991ValMet: 0.991 ± 0.224
2.313ValAsn: 2.313 ± 0.374
3.107ValPro: 3.107 ± 0.43
2.38ValGln: 2.38 ± 0.586
5.751ValArg: 5.751 ± 0.515
3.768ValSer: 3.768 ± 0.496
5.552ValThr: 5.552 ± 0.599
5.618ValVal: 5.618 ± 0.702
0.727ValTrp: 0.727 ± 0.224
1.388ValTyr: 1.388 ± 0.288
0.0ValXaa: 0.0 ± 0.0
Trp
1.719TrpAla: 1.719 ± 0.369
0.397TrpCys: 0.397 ± 0.164
1.19TrpAsp: 1.19 ± 0.351
0.793TrpGlu: 0.793 ± 0.246
0.595TrpPhe: 0.595 ± 0.186
1.322TrpGly: 1.322 ± 0.275
0.793TrpHis: 0.793 ± 0.224
0.727TrpIle: 0.727 ± 0.228
0.529TrpLys: 0.529 ± 0.182
1.52TrpLeu: 1.52 ± 0.31
0.529TrpMet: 0.529 ± 0.167
0.33TrpAsn: 0.33 ± 0.149
1.322TrpPro: 1.322 ± 0.245
0.661TrpGln: 0.661 ± 0.197
2.181TrpArg: 2.181 ± 0.389
1.388TrpSer: 1.388 ± 0.4
1.586TrpThr: 1.586 ± 0.308
0.991TrpVal: 0.991 ± 0.242
0.595TrpTrp: 0.595 ± 0.178
0.397TrpTyr: 0.397 ± 0.178
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.842TyrAla: 2.842 ± 0.421
0.066TyrCys: 0.066 ± 0.065
1.124TyrAsp: 1.124 ± 0.219
1.388TyrGlu: 1.388 ± 0.325
0.661TyrPhe: 0.661 ± 0.196
2.115TyrGly: 2.115 ± 0.292
0.529TyrHis: 0.529 ± 0.229
0.529TyrIle: 0.529 ± 0.235
0.529TyrLys: 0.529 ± 0.208
1.851TyrLeu: 1.851 ± 0.41
0.463TyrMet: 0.463 ± 0.161
0.264TyrAsn: 0.264 ± 0.134
1.124TyrPro: 1.124 ± 0.267
1.058TyrGln: 1.058 ± 0.255
1.454TyrArg: 1.454 ± 0.272
1.719TyrSer: 1.719 ± 0.298
1.19TyrThr: 1.19 ± 0.254
1.388TyrVal: 1.388 ± 0.252
0.132TyrTrp: 0.132 ± 0.094
0.529TyrTyr: 0.529 ± 0.174
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 75 proteins (15130 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski