Proteome-pI 2.0 - Proteome Isoelectric Point Database

Prochlorococcus marinus (strain MIT 9313)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Prochloraceae; Prochlorococcus; Prochlorococcus marinus

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 2830 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|Q7V7H6|Q7V7H6_PROMM Putative modulator of DNA gyrase TldD OS=Prochlorococcus marinus (strain MIT 9313) OX=74547 GN=tldD PE=3 SV=1

MM1 pKa = 7.88DD2 pKa = 3.02VRR4 pKa = 11.84FRR6 pKa = 11.84EE7 pKa = 4.08VDD9 pKa = 3.98PFNCWLWLHH18 pKa = 6.3FADD21 pKa = 5.26VPSQGEE27 pKa = 3.81QNYY30 pKa = 9.85VDD32 pKa = 5.11GIFDD36 pKa = 3.12SWYY39 pKa = 9.81VIGRR43 pKa = 11.84LGGFNAEE50 pKa = 3.77NLQVHH55 pKa = 6.0EE56 pKa = 4.97EE57 pKa = 4.2GADD60 pKa = 3.86LSWITYY66 pKa = 9.97DD67 pKa = 3.97NEE69 pKa = 4.23DD70 pKa = 3.62ARR72 pKa = 11.84SVIPALMHH80 pKa = 6.86NMGQLEE86 pKa = 4.19YY87 pKa = 9.54QGCWARR93 pKa = 11.84CWVDD97 pKa = 4.38LGTSDD102 pKa = 5.67AVALDD107 pKa = 3.48VLINALQQIDD117 pKa = 4.05SDD119 pKa = 4.4VVQLDD124 pKa = 3.51EE125 pKa = 4.27VLIGGVNDD133 pKa = 3.79DD134 pKa = 4.5WPVEE138 pKa = 3.88PHH140 pKa = 7.23PDD142 pKa = 3.33SIFPVEE148 pKa = 3.81AA149 pKa = 4.53

Molecular weight: 16.87 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>sp|Q7V6C6|HEMH_PROMM Ferrochelatase OS=Prochlorococcus marinus (strain MIT 9313) OX=74547 GN=hemH PE=3 SV=1

MM1 pKa = 7.41TKK3 pKa = 9.05RR4 pKa = 11.84TFGGTSRR11 pKa = 11.84KK12 pKa = 9.13RR13 pKa = 11.84KK14 pKa = 8.17RR15 pKa = 11.84VSGFRR20 pKa = 11.84VRR22 pKa = 11.84MRR24 pKa = 11.84NHH26 pKa = 5.79TGRR29 pKa = 11.84RR30 pKa = 11.84VIRR33 pKa = 11.84SRR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.92RR38 pKa = 11.84GRR40 pKa = 11.84ARR42 pKa = 11.84LAVV45 pKa = 3.5

Molecular weight: 5.4 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
2830	0	2830	687533	30	1765	242.9	26.72

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
9.571 ± 0.059	1.363 ± 0.019	5.041 ± 0.043	5.801 ± 0.049	3.282 ± 0.032	7.773 ± 0.047	2.182 ± 0.025	4.938 ± 0.043	3.579 ± 0.045	12.497 ± 0.084

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.202 ± 0.019	3.242 ± 0.039	5.149 ± 0.04	4.869 ± 0.039	6.496 ± 0.046	6.861 ± 0.048	4.724 ± 0.032	6.739 ± 0.04	1.725 ± 0.027	1.964 ± 0.029

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski