Amino acid dipepetide frequency for Prochlorococcus marinus (strain MIT 9313)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.542AlaAla: 10.542 ± 0.167	1.5AlaCys: 1.5 ± 0.041	4.938AlaAsp: 4.938 ± 0.087	5.92AlaGlu: 5.92 ± 0.12	3.124AlaPhe: 3.124 ± 0.069	7.3AlaGly: 7.3 ± 0.128	1.632AlaHis: 1.632 ± 0.055	5.54AlaIle: 5.54 ± 0.115	3.597AlaLys: 3.597 ± 0.089	12.363AlaLeu: 12.363 ± 0.167	2.624AlaMet: 2.624 ± 0.066	2.977AlaAsn: 2.977 ± 0.081	3.751AlaPro: 3.751 ± 0.073	3.635AlaGln: 3.635 ± 0.079	5.29AlaArg: 5.29 ± 0.089	6.208AlaSer: 6.208 ± 0.106	4.369AlaThr: 4.369 ± 0.082	6.862AlaVal: 6.862 ± 0.118	1.609AlaTrp: 1.609 ± 0.059	1.93AlaTyr: 1.93 ± 0.065	0.0AlaXaa: 0.0 ± 0.0
Cys	0.896CysAla: 0.896 ± 0.036	0.34CysCys: 0.34 ± 0.025	0.716CysAsp: 0.716 ± 0.032	0.703CysGlu: 0.703 ± 0.037	0.596CysPhe: 0.596 ± 0.032	1.258CysGly: 1.258 ± 0.046	0.361CysHis: 0.361 ± 0.021	0.583CysIle: 0.583 ± 0.033	0.41CysLys: 0.41 ± 0.026	1.662CysLeu: 1.662 ± 0.051	0.282CysMet: 0.282 ± 0.021	0.423CysAsn: 0.423 ± 0.026	0.682CysPro: 0.682 ± 0.03	0.58CysGln: 0.58 ± 0.031	1.005CysArg: 1.005 ± 0.035	1.121CysSer: 1.121 ± 0.037	0.557CysThr: 0.557 ± 0.028	0.809CysVal: 0.809 ± 0.037	0.351CysTrp: 0.351 ± 0.024	0.291CysTyr: 0.291 ± 0.02	0.0CysXaa: 0.0 ± 0.0
Asp	4.337AspAla: 4.337 ± 0.075	0.694AspCys: 0.694 ± 0.029	2.564AspAsp: 2.564 ± 0.082	2.851AspGlu: 2.851 ± 0.078	1.661AspPhe: 1.661 ± 0.052	4.267AspGly: 4.267 ± 0.096	1.297AspHis: 1.297 ± 0.043	1.886AspIle: 1.886 ± 0.054	1.338AspLys: 1.338 ± 0.051	7.112AspLeu: 7.112 ± 0.116	0.816AspMet: 0.816 ± 0.032	1.357AspAsn: 1.357 ± 0.052	3.446AspPro: 3.446 ± 0.074	3.034AspGln: 3.034 ± 0.068	3.428AspArg: 3.428 ± 0.074	2.992AspSer: 2.992 ± 0.076	1.79AspThr: 1.79 ± 0.053	3.287AspVal: 3.287 ± 0.076	0.99AspTrp: 0.99 ± 0.039	1.257AspTyr: 1.257 ± 0.064	0.0AspXaa: 0.0 ± 0.0
Glu	6.634GluAla: 6.634 ± 0.116	0.516GluCys: 0.516 ± 0.025	2.695GluAsp: 2.695 ± 0.071	3.491GluGlu: 3.491 ± 0.099	1.43GluPhe: 1.43 ± 0.051	4.265GluGly: 4.265 ± 0.075	1.242GluHis: 1.242 ± 0.047	2.861GluIle: 2.861 ± 0.073	2.196GluLys: 2.196 ± 0.066	7.888GluLeu: 7.888 ± 0.131	1.276GluMet: 1.276 ± 0.051	1.518GluAsn: 1.518 ± 0.046	2.702GluPro: 2.702 ± 0.074	3.684GluGln: 3.684 ± 0.094	4.279GluArg: 4.279 ± 0.096	3.286GluSer: 3.286 ± 0.07	2.627GluThr: 2.627 ± 0.067	3.952GluVal: 3.952 ± 0.089	0.745GluTrp: 0.745 ± 0.034	0.729GluTyr: 0.729 ± 0.035	0.0GluXaa: 0.0 ± 0.0
Phe	3.104PheAla: 3.104 ± 0.07	0.614PheCys: 0.614 ± 0.034	2.121PheAsp: 2.121 ± 0.059	1.93PheGlu: 1.93 ± 0.055	1.191PhePhe: 1.191 ± 0.05	2.832PheGly: 2.832 ± 0.065	0.703PheHis: 0.703 ± 0.033	1.436PheIle: 1.436 ± 0.048	1.081PheLys: 1.081 ± 0.044	3.403PheLeu: 3.403 ± 0.082	0.653PheMet: 0.653 ± 0.031	1.36PheAsn: 1.36 ± 0.055	1.406PhePro: 1.406 ± 0.046	1.29PheGln: 1.29 ± 0.04	1.937PheArg: 1.937 ± 0.06	2.557PheSer: 2.557 ± 0.065	1.719PheThr: 1.719 ± 0.054	2.022PheVal: 2.022 ± 0.061	0.637PheTrp: 0.637 ± 0.034	0.826PheTyr: 0.826 ± 0.034	0.0PheXaa: 0.0 ± 0.0
Gly	6.381GlyAla: 6.381 ± 0.113	1.358GlyCys: 1.358 ± 0.05	3.862GlyAsp: 3.862 ± 0.088	4.106GlyGlu: 4.106 ± 0.085	3.172GlyPhe: 3.172 ± 0.068	6.205GlyGly: 6.205 ± 0.147	1.764GlyHis: 1.764 ± 0.053	4.179GlyIle: 4.179 ± 0.091	2.877GlyLys: 2.877 ± 0.068	10.136GlyLeu: 10.136 ± 0.148	1.968GlyMet: 1.968 ± 0.059	2.304GlyAsn: 2.304 ± 0.067	2.955GlyPro: 2.955 ± 0.072	3.338GlyGln: 3.338 ± 0.077	4.91GlyArg: 4.91 ± 0.095	5.514GlySer: 5.514 ± 0.097	3.526GlyThr: 3.526 ± 0.087	5.652GlyVal: 5.652 ± 0.096	1.667GlyTrp: 1.667 ± 0.052	1.852GlyTyr: 1.852 ± 0.053	0.0GlyXaa: 0.0 ± 0.0
His	1.738HisAla: 1.738 ± 0.057	0.384HisCys: 0.384 ± 0.025	1.008HisAsp: 1.008 ± 0.043	0.929HisGlu: 0.929 ± 0.039	0.791HisPhe: 0.791 ± 0.035	1.785HisGly: 1.785 ± 0.063	0.817HisHis: 0.817 ± 0.04	0.784HisIle: 0.784 ± 0.036	0.508HisLys: 0.508 ± 0.027	2.819HisLeu: 2.819 ± 0.069	0.319HisMet: 0.319 ± 0.021	0.675HisAsn: 0.675 ± 0.034	1.61HisPro: 1.61 ± 0.049	1.425HisGln: 1.425 ± 0.047	1.735HisArg: 1.735 ± 0.054	1.383HisSer: 1.383 ± 0.047	0.816HisThr: 0.816 ± 0.038	1.12HisVal: 1.12 ± 0.043	0.591HisTrp: 0.591 ± 0.03	0.58HisTyr: 0.58 ± 0.03	0.0HisXaa: 0.0 ± 0.0
Ile	5.168IleAla: 5.168 ± 0.107	0.748IleCys: 0.748 ± 0.031	3.15IleAsp: 3.15 ± 0.083	3.401IleGlu: 3.401 ± 0.08	1.406IlePhe: 1.406 ± 0.05	4.417IleGly: 4.417 ± 0.088	1.161IleHis: 1.161 ± 0.045	1.764IleIle: 1.764 ± 0.065	2.013IleLys: 2.013 ± 0.056	4.179IleLeu: 4.179 ± 0.085	0.631IleMet: 0.631 ± 0.031	2.161IleAsn: 2.161 ± 0.069	2.78IlePro: 2.78 ± 0.06	2.014IleGln: 2.014 ± 0.053	2.999IleArg: 2.999 ± 0.061	3.745IleSer: 3.745 ± 0.08	2.951IleThr: 2.951 ± 0.071	2.842IleVal: 2.842 ± 0.075	0.797IleTrp: 0.797 ± 0.035	1.056IleTyr: 1.056 ± 0.044	0.0IleXaa: 0.0 ± 0.0
Lys	4.109LysAla: 4.109 ± 0.096	0.288LysCys: 0.288 ± 0.017	1.872LysAsp: 1.872 ± 0.058	2.163LysGlu: 2.163 ± 0.067	0.889LysPhe: 0.889 ± 0.038	2.836LysGly: 2.836 ± 0.085	0.707LysHis: 0.707 ± 0.03	1.495LysIle: 1.495 ± 0.043	1.521LysLys: 1.521 ± 0.054	3.785LysLeu: 3.785 ± 0.086	0.595LysMet: 0.595 ± 0.029	1.126LysAsn: 1.126 ± 0.048	2.172LysPro: 2.172 ± 0.063	2.036LysGln: 2.036 ± 0.053	2.551LysArg: 2.551 ± 0.057	2.284LysSer: 2.284 ± 0.071	1.948LysThr: 1.948 ± 0.051	2.531LysVal: 2.531 ± 0.063	0.276LysTrp: 0.276 ± 0.02	0.604LysTyr: 0.604 ± 0.033	0.0LysXaa: 0.0 ± 0.0
Leu	12.754LeuAla: 12.754 ± 0.2	1.498LeuCys: 1.498 ± 0.05	6.11LeuAsp: 6.11 ± 0.123	8.205LeuGlu: 8.205 ± 0.124	3.555LeuPhe: 3.555 ± 0.087	9.076LeuGly: 9.076 ± 0.136	2.461LeuHis: 2.461 ± 0.067	6.672LeuIle: 6.672 ± 0.113	4.935LeuLys: 4.935 ± 0.091	15.335LeuLeu: 15.335 ± 0.244	3.019LeuMet: 3.019 ± 0.072	4.299LeuAsn: 4.299 ± 0.086	6.797LeuPro: 6.797 ± 0.116	6.147LeuGln: 6.147 ± 0.094	7.896LeuArg: 7.896 ± 0.122	8.292LeuSer: 8.292 ± 0.126	5.629LeuThr: 5.629 ± 0.095	8.627LeuVal: 8.627 ± 0.128	1.812LeuTrp: 1.812 ± 0.064	1.854LeuTyr: 1.854 ± 0.056	0.0LeuXaa: 0.0 ± 0.0
Met	2.945MetAla: 2.945 ± 0.068	0.14MetCys: 0.14 ± 0.013	0.97MetAsp: 0.97 ± 0.036	1.178MetGlu: 1.178 ± 0.041	0.515MetPhe: 0.515 ± 0.026	1.847MetGly: 1.847 ± 0.055	0.413MetHis: 0.413 ± 0.025	1.04MetIle: 1.04 ± 0.041	0.78MetLys: 0.78 ± 0.035	2.375MetLeu: 2.375 ± 0.059	0.388MetMet: 0.388 ± 0.022	0.751MetAsn: 0.751 ± 0.032	1.372MetPro: 1.372 ± 0.045	0.967MetGln: 0.967 ± 0.037	1.312MetArg: 1.312 ± 0.047	1.609MetSer: 1.609 ± 0.048	1.348MetThr: 1.348 ± 0.044	1.742MetVal: 1.742 ± 0.051	0.118MetTrp: 0.118 ± 0.014	0.208MetTyr: 0.208 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	2.842AsnAla: 2.842 ± 0.072	0.46AsnCys: 0.46 ± 0.026	1.673AsnAsp: 1.673 ± 0.055	1.53AsnGlu: 1.53 ± 0.044	1.036AsnPhe: 1.036 ± 0.038	2.563AsnGly: 2.563 ± 0.064	0.909AsnHis: 0.909 ± 0.034	1.31AsnIle: 1.31 ± 0.049	1.22AsnLys: 1.22 ± 0.049	3.851AsnLeu: 3.851 ± 0.096	0.535AsnMet: 0.535 ± 0.028	1.258AsnAsn: 1.258 ± 0.055	2.214AsnPro: 2.214 ± 0.058	1.779AsnGln: 1.779 ± 0.055	2.279AsnArg: 2.279 ± 0.064	2.269AsnSer: 2.269 ± 0.097	1.603AsnThr: 1.603 ± 0.054	1.731AsnVal: 1.731 ± 0.051	0.583AsnTrp: 0.583 ± 0.03	0.78AsnTyr: 0.78 ± 0.036	0.0AsnXaa: 0.0 ± 0.0
Pro	4.519ProAla: 4.519 ± 0.085	0.598ProCys: 0.598 ± 0.029	2.803ProAsp: 2.803 ± 0.076	3.657ProGlu: 3.657 ± 0.078	1.825ProPhe: 1.825 ± 0.054	3.786ProGly: 3.786 ± 0.077	1.025ProHis: 1.025 ± 0.041	2.8ProIle: 2.8 ± 0.069	1.833ProLys: 1.833 ± 0.062	6.563ProLeu: 6.563 ± 0.123	1.261ProMet: 1.261 ± 0.04	1.601ProAsn: 1.601 ± 0.045	2.316ProPro: 2.316 ± 0.068	2.265ProGln: 2.265 ± 0.063	2.631ProArg: 2.631 ± 0.065	3.854ProSer: 3.854 ± 0.079	2.531ProThr: 2.531 ± 0.064	3.591ProVal: 3.591 ± 0.078	0.967ProTrp: 0.967 ± 0.039	1.069ProTyr: 1.069 ± 0.041	0.0ProXaa: 0.0 ± 0.0
Gln	4.964GlnAla: 4.964 ± 0.099	0.492GlnCys: 0.492 ± 0.025	1.891GlnAsp: 1.891 ± 0.053	2.822GlnGlu: 2.822 ± 0.078	1.212GlnPhe: 1.212 ± 0.042	3.526GlnGly: 3.526 ± 0.068	1.082GlnHis: 1.082 ± 0.042	2.22GlnIle: 2.22 ± 0.062	1.651GlnLys: 1.651 ± 0.053	6.98GlnLeu: 6.98 ± 0.121	0.957GlnMet: 0.957 ± 0.037	1.249GlnAsn: 1.249 ± 0.049	2.714GlnPro: 2.714 ± 0.065	3.316GlnGln: 3.316 ± 0.092	3.975GlnArg: 3.975 ± 0.092	2.942GlnSer: 2.942 ± 0.078	2.044GlnThr: 2.044 ± 0.056	3.225GlnVal: 3.225 ± 0.074	0.865GlnTrp: 0.865 ± 0.037	0.569GlnTyr: 0.569 ± 0.034	0.0GlnXaa: 0.0 ± 0.0
Arg	4.707ArgAla: 4.707 ± 0.084	1.015ArgCys: 1.015 ± 0.045	3.008ArgAsp: 3.008 ± 0.065	3.449ArgGlu: 3.449 ± 0.077	2.705ArgPhe: 2.705 ± 0.063	4.122ArgGly: 4.122 ± 0.09	1.686ArgHis: 1.686 ± 0.053	3.281ArgIle: 3.281 ± 0.066	2.141ArgLys: 2.141 ± 0.066	8.936ArgLeu: 8.936 ± 0.135	1.449ArgMet: 1.449 ± 0.047	1.993ArgAsn: 1.993 ± 0.065	3.117ArgPro: 3.117 ± 0.078	3.828ArgGln: 3.828 ± 0.088	5.236ArgArg: 5.236 ± 0.103	4.551ArgSer: 4.551 ± 0.094	2.688ArgThr: 2.688 ± 0.06	3.923ArgVal: 3.923 ± 0.094	1.578ArgTrp: 1.578 ± 0.053	1.549ArgTyr: 1.549 ± 0.055	0.0ArgXaa: 0.0 ± 0.0
Ser	5.546SerAla: 5.546 ± 0.104	1.04SerCys: 1.04 ± 0.045	3.392SerAsp: 3.392 ± 0.071	3.606SerGlu: 3.606 ± 0.083	2.537SerPhe: 2.537 ± 0.06	5.614SerGly: 5.614 ± 0.099	1.516SerHis: 1.516 ± 0.048	3.446SerIle: 3.446 ± 0.074	2.729SerLys: 2.729 ± 0.081	8.34SerLeu: 8.34 ± 0.13	1.737SerMet: 1.737 ± 0.052	2.444SerAsn: 2.444 ± 0.082	3.268SerPro: 3.268 ± 0.069	3.027SerGln: 3.027 ± 0.065	4.273SerArg: 4.273 ± 0.08	5.648SerSer: 5.648 ± 0.142	3.619SerThr: 3.619 ± 0.076	4.02SerVal: 4.02 ± 0.079	1.347SerTrp: 1.347 ± 0.047	1.462SerTyr: 1.462 ± 0.069	0.0SerXaa: 0.0 ± 0.0
Thr	4.867ThrAla: 4.867 ± 0.09	0.564ThrCys: 0.564 ± 0.028	2.316ThrAsp: 2.316 ± 0.061	2.234ThrGlu: 2.234 ± 0.067	1.606ThrPhe: 1.606 ± 0.048	3.818ThrGly: 3.818 ± 0.1	0.925ThrHis: 0.925 ± 0.041	2.516ThrIle: 2.516 ± 0.058	1.546ThrLys: 1.546 ± 0.053	5.54ThrLeu: 5.54 ± 0.087	1.004ThrMet: 1.004 ± 0.038	1.524ThrAsn: 1.524 ± 0.052	3.139ThrPro: 3.139 ± 0.074	1.646ThrGln: 1.646 ± 0.049	2.537ThrArg: 2.537 ± 0.066	3.537ThrSer: 3.537 ± 0.073	2.948ThrThr: 2.948 ± 0.076	3.22ThrVal: 3.22 ± 0.075	0.647ThrTrp: 0.647 ± 0.033	1.11ThrTyr: 1.11 ± 0.044	0.0ThrXaa: 0.0 ± 0.0
Val	6.787ValAla: 6.787 ± 0.121	0.8ValCys: 0.8 ± 0.037	3.667ValAsp: 3.667 ± 0.081	4.186ValGlu: 4.186 ± 0.088	2.282ValPhe: 2.282 ± 0.058	5.003ValGly: 5.003 ± 0.092	1.268ValHis: 1.268 ± 0.044	3.824ValIle: 3.824 ± 0.081	2.224ValLys: 2.224 ± 0.057	8.624ValLeu: 8.624 ± 0.128	1.692ValMet: 1.692 ± 0.054	2.244ValAsn: 2.244 ± 0.058	3.092ValPro: 3.092 ± 0.071	2.602ValGln: 2.602 ± 0.063	3.747ValArg: 3.747 ± 0.079	4.273ValSer: 4.273 ± 0.085	3.012ValThr: 3.012 ± 0.073	6.09ValVal: 6.09 ± 0.125	0.839ValTrp: 0.839 ± 0.033	1.134ValTyr: 1.134 ± 0.035	0.0ValXaa: 0.0 ± 0.0
Trp	1.267TrpAla: 1.267 ± 0.05	0.247TrpCys: 0.247 ± 0.02	0.663TrpAsp: 0.663 ± 0.031	0.646TrpGlu: 0.646 ± 0.033	0.612TrpPhe: 0.612 ± 0.03	1.161TrpGly: 1.161 ± 0.04	0.448TrpHis: 0.448 ± 0.025	0.986TrpIle: 0.986 ± 0.035	0.534TrpLys: 0.534 ± 0.029	2.801TrpLeu: 2.801 ± 0.075	0.489TrpMet: 0.489 ± 0.03	0.531TrpAsn: 0.531 ± 0.025	0.927TrpPro: 0.927 ± 0.047	1.161TrpGln: 1.161 ± 0.044	1.324TrpArg: 1.324 ± 0.047	1.108TrpSer: 1.108 ± 0.042	0.676TrpThr: 0.676 ± 0.036	0.954TrpVal: 0.954 ± 0.043	0.419TrpTrp: 0.419 ± 0.03	0.301TrpTyr: 0.301 ± 0.023	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.604TyrAla: 1.604 ± 0.053	0.332TyrCys: 0.332 ± 0.018	0.989TyrAsp: 0.989 ± 0.051	1.011TyrGlu: 1.011 ± 0.04	0.676TyrPhe: 0.676 ± 0.034	2.045TyrGly: 2.045 ± 0.057	0.401TyrHis: 0.401 ± 0.026	0.761TyrIle: 0.761 ± 0.031	0.653TyrLys: 0.653 ± 0.032	2.281TyrLeu: 2.281 ± 0.053	0.323TyrMet: 0.323 ± 0.02	0.628TyrAsn: 0.628 ± 0.048	1.036TyrPro: 1.036 ± 0.039	0.919TyrGln: 0.919 ± 0.042	1.616TyrArg: 1.616 ± 0.046	1.434TyrSer: 1.434 ± 0.059	0.844TyrThr: 0.844 ± 0.044	1.191TyrVal: 1.191 ± 0.044	0.416TyrTrp: 0.416 ± 0.024	0.484TyrTyr: 0.484 ± 0.026	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2830 proteins (687533 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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