Candidatus Nitrosopumilus salaria BD31

Taxonomy: cellular organisms; Archaea; TACK group; Thaumarchaeota; Nitrosopumilales; Nitrosopumilaceae; Nitrosopumilus; Candidatus Nitrosopumilus salaria

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2154 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I3D316|I3D316_9ARCH Hydrolase carbon-nitrogen family OS=Candidatus Nitrosopumilus salaria BD31 OX=859350 GN=BD31_I0601 PE=3 SV=1
MM1 pKa = 7.64ANFVIDD7 pKa = 4.05KK8 pKa = 10.26KK9 pKa = 10.84IKK11 pKa = 10.42LVLALLGAIICLSFLAWSEE30 pKa = 3.8DD31 pKa = 3.79RR32 pKa = 11.84IFADD36 pKa = 4.47FDD38 pKa = 3.82NDD40 pKa = 5.09GITDD44 pKa = 3.71SDD46 pKa = 4.75DD47 pKa = 3.75NCPLNANLAQTDD59 pKa = 3.34SDD61 pKa = 4.36YY62 pKa = 11.26DD63 pKa = 4.01TVGDD67 pKa = 4.33EE68 pKa = 5.72CDD70 pKa = 3.71TDD72 pKa = 3.98DD73 pKa = 6.24DD74 pKa = 4.71NDD76 pKa = 4.18GVSDD80 pKa = 4.99SLDD83 pKa = 3.34QFDD86 pKa = 5.11TNPLDD91 pKa = 3.4WADD94 pKa = 3.85FDD96 pKa = 4.93FDD98 pKa = 5.64GIGSSQDD105 pKa = 3.09TDD107 pKa = 3.95DD108 pKa = 5.57DD109 pKa = 4.37NDD111 pKa = 5.21GILDD115 pKa = 4.01TNDD118 pKa = 4.03SYY120 pKa = 12.16PMTASEE126 pKa = 4.31ILATKK131 pKa = 10.45YY132 pKa = 10.63LDD134 pKa = 4.39NIEE137 pKa = 4.35TCAEE141 pKa = 4.17MNDD144 pKa = 3.14GTMRR148 pKa = 11.84LVCYY152 pKa = 10.59SEE154 pKa = 4.83FFGTLTEE161 pKa = 4.13NEE163 pKa = 4.61KK164 pKa = 11.18NNSDD168 pKa = 3.73ALEE171 pKa = 4.01LSIALSKK178 pKa = 10.65IGAIDD183 pKa = 3.76DD184 pKa = 3.93CHH186 pKa = 6.39FVSHH190 pKa = 7.06EE191 pKa = 4.07IGHH194 pKa = 5.98VSYY197 pKa = 11.32EE198 pKa = 3.93MTTDD202 pKa = 3.31VTKK205 pKa = 10.73SLQGMDD211 pKa = 2.98GTMCRR216 pKa = 11.84GGYY219 pKa = 8.53FHH221 pKa = 7.3GVLSSYY227 pKa = 9.48FHH229 pKa = 7.04NIEE232 pKa = 4.07EE233 pKa = 4.58LGKK236 pKa = 10.34SFPDD240 pKa = 3.7SYY242 pKa = 10.79QTICDD247 pKa = 4.02DD248 pKa = 5.63LIGSSNYY255 pKa = 8.91QDD257 pKa = 3.97CVHH260 pKa = 6.48GLGHH264 pKa = 6.7GFVHH268 pKa = 6.75YY269 pKa = 10.57FGDD272 pKa = 4.16DD273 pKa = 3.51LNSSLEE279 pKa = 4.18SCHH282 pKa = 7.11DD283 pKa = 3.55LSFYY287 pKa = 11.28QNILCVKK294 pKa = 9.15GVMMQYY300 pKa = 9.34TDD302 pKa = 3.97NVITRR307 pKa = 11.84NGMSKK312 pKa = 10.68DD313 pKa = 3.67VLSNLCDD320 pKa = 3.61SEE322 pKa = 4.16QLEE325 pKa = 4.08RR326 pKa = 11.84FDD328 pKa = 5.28NVEE331 pKa = 4.29CSMSTGTTLAFFTNHH346 pKa = 6.52DD347 pKa = 3.79FEE349 pKa = 6.24KK350 pKa = 11.0GKK352 pKa = 9.24EE353 pKa = 4.07LCNMIEE359 pKa = 4.49DD360 pKa = 4.01ADD362 pKa = 3.9SRR364 pKa = 11.84NYY366 pKa = 10.41CIEE369 pKa = 3.78GLRR372 pKa = 11.84LEE374 pKa = 4.53IQDD377 pKa = 3.78SEE379 pKa = 4.84KK380 pKa = 11.32YY381 pKa = 9.85EE382 pKa = 4.49SNPLTEE388 pKa = 5.54DD389 pKa = 2.8IRR391 pKa = 11.84EE392 pKa = 4.04KK393 pKa = 10.6FQPQFIEE400 pKa = 4.63GSKK403 pKa = 10.74SIDD406 pKa = 3.04IRR408 pKa = 11.84SPAIISDD415 pKa = 3.66FQVMKK420 pKa = 10.02EE421 pKa = 3.79VGVISFSIDD430 pKa = 2.7KK431 pKa = 8.61PQYY434 pKa = 8.45VILYY438 pKa = 8.84IPSEE442 pKa = 4.2FVASKK447 pKa = 10.27MLVTVNGQIPMDD459 pKa = 4.85LKK461 pKa = 11.13SQDD464 pKa = 3.6YY465 pKa = 11.15LLGKK469 pKa = 9.13EE470 pKa = 3.76MAMISFVPNQSGTVLITPVQQ490 pKa = 3.28

Molecular weight: 54.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I3D1L4|I3D1L4_9ARCH Ribosomal RNA large subunit methyltransferase E OS=Candidatus Nitrosopumilus salaria BD31 OX=859350 GN=rrmJ PE=3 SV=1
MM1 pKa = 7.04VVSKK5 pKa = 10.99AKK7 pKa = 10.13RR8 pKa = 11.84AEE10 pKa = 4.02AAKK13 pKa = 10.22KK14 pKa = 9.66AARR17 pKa = 11.84TRR19 pKa = 11.84KK20 pKa = 9.74RR21 pKa = 11.84NAAKK25 pKa = 10.23AAAEE29 pKa = 3.95ALKK32 pKa = 10.63AAAARR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.31AAAARR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84NATKK51 pKa = 10.26AAPKK55 pKa = 10.04RR56 pKa = 11.84KK57 pKa = 8.53AAKK60 pKa = 9.87RR61 pKa = 11.84RR62 pKa = 11.84TAAKK66 pKa = 9.79KK67 pKa = 9.68AAPKK71 pKa = 10.23RR72 pKa = 11.84KK73 pKa = 8.66AAKK76 pKa = 9.87RR77 pKa = 11.84KK78 pKa = 8.54AAPKK82 pKa = 10.14RR83 pKa = 11.84RR84 pKa = 11.84TAAKK88 pKa = 9.74KK89 pKa = 9.68AAPKK93 pKa = 10.23RR94 pKa = 11.84KK95 pKa = 8.66AAKK98 pKa = 9.87RR99 pKa = 11.84KK100 pKa = 8.81AAPKK104 pKa = 10.02RR105 pKa = 11.84KK106 pKa = 8.69AAKK109 pKa = 9.87RR110 pKa = 11.84KK111 pKa = 8.81AAPKK115 pKa = 9.85RR116 pKa = 11.84KK117 pKa = 8.42AAKK120 pKa = 9.71RR121 pKa = 11.84RR122 pKa = 11.84RR123 pKa = 3.63

Molecular weight: 13.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2154

0

2154

469320

30

1691

217.9

24.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.862 ± 0.064

1.099 ± 0.027

5.926 ± 0.061

6.952 ± 0.056

4.478 ± 0.053

6.388 ± 0.077

1.854 ± 0.027

8.987 ± 0.064

8.586 ± 0.083

8.659 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.669 ± 0.036

5.09 ± 0.046

3.711 ± 0.04

3.181 ± 0.033

3.582 ± 0.045

7.235 ± 0.058

5.396 ± 0.047

6.303 ± 0.046

0.932 ± 0.019

3.11 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski