Amino acid dipepetide frequency for Candidatus Nitrosopumilus salaria BD31

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.253AlaAla: 4.253 ± 0.144
0.652AlaCys: 0.652 ± 0.041
3.06AlaAsp: 3.06 ± 0.079
3.752AlaGlu: 3.752 ± 0.086
2.523AlaPhe: 2.523 ± 0.077
4.063AlaGly: 4.063 ± 0.1
1.046AlaHis: 1.046 ± 0.049
5.431AlaIle: 5.431 ± 0.119
5.538AlaLys: 5.538 ± 0.127
5.378AlaLeu: 5.378 ± 0.135
1.587AlaMet: 1.587 ± 0.054
2.58AlaAsn: 2.58 ± 0.078
1.747AlaPro: 1.747 ± 0.067
1.805AlaGln: 1.805 ± 0.067
2.237AlaArg: 2.237 ± 0.078
3.878AlaSer: 3.878 ± 0.09
3.149AlaThr: 3.149 ± 0.086
3.712AlaVal: 3.712 ± 0.104
0.535AlaTrp: 0.535 ± 0.037
1.69AlaTyr: 1.69 ± 0.06
0.002AlaXaa: 0.002 ± 0.002
Cys
0.556CysAla: 0.556 ± 0.039
0.145CysCys: 0.145 ± 0.018
0.716CysAsp: 0.716 ± 0.042
0.637CysGlu: 0.637 ± 0.039
0.394CysPhe: 0.394 ± 0.03
1.119CysGly: 1.119 ± 0.054
0.249CysHis: 0.249 ± 0.021
0.727CysIle: 0.727 ± 0.039
0.969CysLys: 0.969 ± 0.053
0.707CysLeu: 0.707 ± 0.041
0.254CysMet: 0.254 ± 0.023
0.607CysAsn: 0.607 ± 0.034
0.797CysPro: 0.797 ± 0.114
0.324CysGln: 0.324 ± 0.025
0.396CysArg: 0.396 ± 0.03
0.729CysSer: 0.729 ± 0.043
0.573CysThr: 0.573 ± 0.036
0.652CysVal: 0.652 ± 0.038
0.092CysTrp: 0.092 ± 0.014
0.345CysTyr: 0.345 ± 0.03
0.0CysXaa: 0.0 ± 0.0
Asp
3.754AspAla: 3.754 ± 0.097
0.577AspCys: 0.577 ± 0.031
3.601AspAsp: 3.601 ± 0.108
4.7AspGlu: 4.7 ± 0.108
2.985AspPhe: 2.985 ± 0.092
4.08AspGly: 4.08 ± 0.174
0.967AspHis: 0.967 ± 0.045
4.758AspIle: 4.758 ± 0.1
4.306AspLys: 4.306 ± 0.104
5.061AspLeu: 5.061 ± 0.11
1.472AspMet: 1.472 ± 0.05
2.593AspAsn: 2.593 ± 0.073
2.386AspPro: 2.386 ± 0.082
1.572AspGln: 1.572 ± 0.065
1.979AspArg: 1.979 ± 0.068
4.5AspSer: 4.5 ± 0.111
2.872AspThr: 2.872 ± 0.081
4.259AspVal: 4.259 ± 0.092
0.622AspTrp: 0.622 ± 0.039
2.212AspTyr: 2.212 ± 0.074
0.002AspXaa: 0.002 ± 0.002
Glu
3.309GluAla: 3.309 ± 0.098
0.652GluCys: 0.652 ± 0.034
3.507GluAsp: 3.507 ± 0.104
4.771GluGlu: 4.771 ± 0.114
3.433GluPhe: 3.433 ± 0.09
3.22GluGly: 3.22 ± 0.074
1.34GluHis: 1.34 ± 0.056
7.492GluIle: 7.492 ± 0.149
7.76GluLys: 7.76 ± 0.163
6.122GluLeu: 6.122 ± 0.129
1.956GluMet: 1.956 ± 0.065
4.332GluAsn: 4.332 ± 0.105
2.154GluPro: 2.154 ± 0.099
2.301GluGln: 2.301 ± 0.07
2.444GluArg: 2.444 ± 0.077
4.843GluSer: 4.843 ± 0.092
3.32GluThr: 3.32 ± 0.087
3.535GluVal: 3.535 ± 0.092
0.695GluTrp: 0.695 ± 0.037
2.331GluTyr: 2.331 ± 0.072
0.0GluXaa: 0.0 ± 0.0
Phe
2.972PheAla: 2.972 ± 0.093
0.584PheCys: 0.584 ± 0.035
3.335PheAsp: 3.335 ± 0.089
3.311PheGlu: 3.311 ± 0.079
1.997PhePhe: 1.997 ± 0.083
3.332PheGly: 3.332 ± 0.103
0.861PheHis: 0.861 ± 0.039
2.815PheIle: 2.815 ± 0.093
2.64PheLys: 2.64 ± 0.063
4.034PheLeu: 4.034 ± 0.108
1.065PheMet: 1.065 ± 0.048
1.922PheAsn: 1.922 ± 0.07
1.624PhePro: 1.624 ± 0.064
1.44PheGln: 1.44 ± 0.062
1.432PheArg: 1.432 ± 0.056
3.661PheSer: 3.661 ± 0.11
2.489PheThr: 2.489 ± 0.076
3.347PheVal: 3.347 ± 0.097
0.422PheTrp: 0.422 ± 0.031
1.496PheTyr: 1.496 ± 0.054
0.0PheXaa: 0.0 ± 0.0
Gly
3.765GlyAla: 3.765 ± 0.095
0.816GlyCys: 0.816 ± 0.064
3.484GlyAsp: 3.484 ± 0.109
3.658GlyGlu: 3.658 ± 0.095
3.177GlyPhe: 3.177 ± 0.091
4.244GlyGly: 4.244 ± 0.144
1.249GlyHis: 1.249 ± 0.053
6.784GlyIle: 6.784 ± 0.134
5.487GlyLys: 5.487 ± 0.122
5.604GlyLeu: 5.604 ± 0.116
1.924GlyMet: 1.924 ± 0.08
2.87GlyAsn: 2.87 ± 0.083
1.847GlyPro: 1.847 ± 0.136
1.713GlyGln: 1.713 ± 0.065
2.291GlyArg: 2.291 ± 0.076
4.136GlySer: 4.136 ± 0.1
3.582GlyThr: 3.582 ± 0.093
4.321GlyVal: 4.321 ± 0.096
0.682GlyTrp: 0.682 ± 0.038
2.242GlyTyr: 2.242 ± 0.075
0.002GlyXaa: 0.002 ± 0.002
His
1.157HisAla: 1.157 ± 0.049
0.243HisCys: 0.243 ± 0.018
1.108HisAsp: 1.108 ± 0.053
1.283HisGlu: 1.283 ± 0.049
0.808HisPhe: 0.808 ± 0.052
1.283HisGly: 1.283 ± 0.054
0.488HisHis: 0.488 ± 0.037
1.366HisIle: 1.366 ± 0.051
1.151HisLys: 1.151 ± 0.052
1.677HisLeu: 1.677 ± 0.061
0.467HisMet: 0.467 ± 0.036
0.867HisAsn: 0.867 ± 0.047
1.04HisPro: 1.04 ± 0.047
0.616HisGln: 0.616 ± 0.038
0.665HisArg: 0.665 ± 0.05
1.183HisSer: 1.183 ± 0.047
0.98HisThr: 0.98 ± 0.053
1.323HisVal: 1.323 ± 0.061
0.185HisTrp: 0.185 ± 0.018
0.654HisTyr: 0.654 ± 0.037
0.0HisXaa: 0.0 ± 0.0
Ile
5.744IleAla: 5.744 ± 0.13
0.865IleCys: 0.865 ± 0.042
5.376IleAsp: 5.376 ± 0.106
6.06IleGlu: 6.06 ± 0.138
3.765IlePhe: 3.765 ± 0.101
5.883IleGly: 5.883 ± 0.117
1.675IleHis: 1.675 ± 0.056
7.856IleIle: 7.856 ± 0.165
6.944IleLys: 6.944 ± 0.143
8.084IleLeu: 8.084 ± 0.148
2.173IleMet: 2.173 ± 0.076
3.955IleAsn: 3.955 ± 0.085
4.351IlePro: 4.351 ± 0.104
2.992IleGln: 2.992 ± 0.084
3.173IleArg: 3.173 ± 0.086
7.296IleSer: 7.296 ± 0.133
5.265IleThr: 5.265 ± 0.115
5.732IleVal: 5.732 ± 0.109
0.629IleTrp: 0.629 ± 0.037
2.058IleTyr: 2.058 ± 0.07
0.0IleXaa: 0.0 ± 0.0
Lys
4.253LysAla: 4.253 ± 0.136
0.94LysCys: 0.94 ± 0.046
4.345LysAsp: 4.345 ± 0.106
6.471LysGlu: 6.471 ± 0.15
3.584LysPhe: 3.584 ± 0.088
4.095LysGly: 4.095 ± 0.12
1.434LysHis: 1.434 ± 0.052
9.714LysIle: 9.714 ± 0.181
8.76LysLys: 8.76 ± 0.191
7.051LysLeu: 7.051 ± 0.142
2.561LysMet: 2.561 ± 0.071
5.531LysAsn: 5.531 ± 0.131
2.706LysPro: 2.706 ± 0.08
2.732LysGln: 2.732 ± 0.085
3.006LysArg: 3.006 ± 0.085
5.885LysSer: 5.885 ± 0.149
4.95LysThr: 4.95 ± 0.109
4.458LysVal: 4.458 ± 0.105
0.678LysTrp: 0.678 ± 0.037
2.706LysTyr: 2.706 ± 0.081
0.0LysXaa: 0.0 ± 0.0
Leu
5.455LeuAla: 5.455 ± 0.116
0.85LeuCys: 0.85 ± 0.045
5.783LeuAsp: 5.783 ± 0.134
6.944LeuGlu: 6.944 ± 0.145
3.603LeuPhe: 3.603 ± 0.091
5.868LeuGly: 5.868 ± 0.113
1.464LeuHis: 1.464 ± 0.058
6.573LeuIle: 6.573 ± 0.122
7.49LeuLys: 7.49 ± 0.167
7.034LeuLeu: 7.034 ± 0.135
2.112LeuMet: 2.112 ± 0.075
4.202LeuAsn: 4.202 ± 0.096
3.224LeuPro: 3.224 ± 0.079
2.81LeuGln: 2.81 ± 0.073
3.409LeuArg: 3.409 ± 0.089
6.716LeuSer: 6.716 ± 0.117
4.487LeuThr: 4.487 ± 0.098
5.553LeuVal: 5.553 ± 0.127
0.688LeuTrp: 0.688 ± 0.044
2.325LeuTyr: 2.325 ± 0.086
0.0LeuXaa: 0.0 ± 0.0
Met
1.76MetAla: 1.76 ± 0.069
0.219MetCys: 0.219 ± 0.022
1.485MetAsp: 1.485 ± 0.065
1.545MetGlu: 1.545 ± 0.047
0.959MetPhe: 0.959 ± 0.047
1.758MetGly: 1.758 ± 0.068
0.526MetHis: 0.526 ± 0.035
2.516MetIle: 2.516 ± 0.075
2.467MetLys: 2.467 ± 0.069
2.088MetLeu: 2.088 ± 0.071
0.929MetMet: 0.929 ± 0.089
1.483MetAsn: 1.483 ± 0.067
1.021MetPro: 1.021 ± 0.05
0.837MetGln: 0.837 ± 0.046
1.012MetArg: 1.012 ± 0.049
1.903MetSer: 1.903 ± 0.062
1.583MetThr: 1.583 ± 0.057
1.643MetVal: 1.643 ± 0.063
0.237MetTrp: 0.237 ± 0.028
0.714MetTyr: 0.714 ± 0.04
0.0MetXaa: 0.0 ± 0.0
Asn
2.877AsnAla: 2.877 ± 0.086
0.629AsnCys: 0.629 ± 0.041
2.776AsnAsp: 2.776 ± 0.09
3.426AsnGlu: 3.426 ± 0.097
2.372AsnPhe: 2.372 ± 0.088
2.947AsnGly: 2.947 ± 0.092
0.963AsnHis: 0.963 ± 0.043
4.089AsnIle: 4.089 ± 0.09
3.644AsnLys: 3.644 ± 0.101
4.809AsnLeu: 4.809 ± 0.113
1.474AsnMet: 1.474 ± 0.056
2.738AsnAsn: 2.738 ± 0.085
2.489AsnPro: 2.489 ± 0.075
2.009AsnGln: 2.009 ± 0.069
1.722AsnArg: 1.722 ± 0.07
4.093AsnSer: 4.093 ± 0.109
2.551AsnThr: 2.551 ± 0.075
3.043AsnVal: 3.043 ± 0.075
0.482AsnTrp: 0.482 ± 0.033
1.773AsnTyr: 1.773 ± 0.06
0.0AsnXaa: 0.0 ± 0.0
Pro
1.96ProAla: 1.96 ± 0.072
0.29ProCys: 0.29 ± 0.027
2.589ProAsp: 2.589 ± 0.105
3.183ProGlu: 3.183 ± 0.093
1.617ProPhe: 1.617 ± 0.051
2.227ProGly: 2.227 ± 0.075
0.748ProHis: 0.748 ± 0.045
3.171ProIle: 3.171 ± 0.082
3.092ProLys: 3.092 ± 0.095
2.898ProLeu: 2.898 ± 0.075
0.816ProMet: 0.816 ± 0.047
1.96ProAsn: 1.96 ± 0.071
1.221ProPro: 1.221 ± 0.053
1.287ProGln: 1.287 ± 0.06
1.229ProArg: 1.229 ± 0.056
2.576ProSer: 2.576 ± 0.073
2.229ProThr: 2.229 ± 0.075
2.489ProVal: 2.489 ± 0.085
0.369ProTrp: 0.369 ± 0.027
1.159ProTyr: 1.159 ± 0.052
0.0ProXaa: 0.0 ± 0.0
Gln
1.666GlnAla: 1.666 ± 0.068
0.373GlnCys: 0.373 ± 0.031
1.594GlnAsp: 1.594 ± 0.063
2.09GlnGlu: 2.09 ± 0.069
1.347GlnPhe: 1.347 ± 0.063
1.739GlnGly: 1.739 ± 0.08
0.584GlnHis: 0.584 ± 0.034
3.358GlnIle: 3.358 ± 0.089
3.326GlnLys: 3.326 ± 0.09
2.591GlnLeu: 2.591 ± 0.083
0.94GlnMet: 0.94 ± 0.056
1.96GlnAsn: 1.96 ± 0.063
0.808GlnPro: 0.808 ± 0.038
1.125GlnGln: 1.125 ± 0.054
1.21GlnArg: 1.21 ± 0.051
2.118GlnSer: 2.118 ± 0.07
1.773GlnThr: 1.773 ± 0.074
1.847GlnVal: 1.847 ± 0.074
0.298GlnTrp: 0.298 ± 0.024
1.061GlnTyr: 1.061 ± 0.049
0.0GlnXaa: 0.0 ± 0.0
Arg
1.886ArgAla: 1.886 ± 0.063
0.46ArgCys: 0.46 ± 0.033
2.203ArgAsp: 2.203 ± 0.073
2.546ArgGlu: 2.546 ± 0.079
1.596ArgPhe: 1.596 ± 0.062
2.086ArgGly: 2.086 ± 0.067
0.618ArgHis: 0.618 ± 0.038
3.441ArgIle: 3.441 ± 0.08
3.36ArgLys: 3.36 ± 0.098
3.286ArgLeu: 3.286 ± 0.085
1.014ArgMet: 1.014 ± 0.048
1.916ArgAsn: 1.916 ± 0.069
1.183ArgPro: 1.183 ± 0.051
1.108ArgGln: 1.108 ± 0.056
1.504ArgArg: 1.504 ± 0.059
2.118ArgSer: 2.118 ± 0.066
1.764ArgThr: 1.764 ± 0.067
2.131ArgVal: 2.131 ± 0.065
0.373ArgTrp: 0.373 ± 0.031
1.227ArgTyr: 1.227 ± 0.055
0.0ArgXaa: 0.0 ± 0.0
Ser
3.797SerAla: 3.797 ± 0.104
0.769SerCys: 0.769 ± 0.048
4.556SerAsp: 4.556 ± 0.105
5.271SerGlu: 5.271 ± 0.13
3.224SerPhe: 3.224 ± 0.097
4.997SerGly: 4.997 ± 0.111
1.327SerHis: 1.327 ± 0.043
6.422SerIle: 6.422 ± 0.133
6.688SerLys: 6.688 ± 0.144
6.226SerLeu: 6.226 ± 0.118
1.96SerMet: 1.96 ± 0.059
3.693SerAsn: 3.693 ± 0.107
2.386SerPro: 2.386 ± 0.077
2.35SerGln: 2.35 ± 0.079
2.408SerArg: 2.408 ± 0.072
5.222SerSer: 5.222 ± 0.139
3.784SerThr: 3.784 ± 0.089
4.43SerVal: 4.43 ± 0.095
0.718SerTrp: 0.718 ± 0.044
2.122SerTyr: 2.122 ± 0.073
0.0SerXaa: 0.0 ± 0.0
Thr
3.237ThrAla: 3.237 ± 0.097
0.592ThrCys: 0.592 ± 0.036
3.038ThrAsp: 3.038 ± 0.095
3.292ThrGlu: 3.292 ± 0.089
2.406ThrPhe: 2.406 ± 0.08
3.893ThrGly: 3.893 ± 0.099
0.948ThrHis: 0.948 ± 0.047
4.899ThrIle: 4.899 ± 0.1
4.415ThrLys: 4.415 ± 0.092
4.756ThrLeu: 4.756 ± 0.105
1.293ThrMet: 1.293 ± 0.052
2.742ThrAsn: 2.742 ± 0.091
2.378ThrPro: 2.378 ± 0.072
1.711ThrGln: 1.711 ± 0.064
1.824ThrArg: 1.824 ± 0.068
3.797ThrSer: 3.797 ± 0.093
2.908ThrThr: 2.908 ± 0.096
3.68ThrVal: 3.68 ± 0.102
0.516ThrTrp: 0.516 ± 0.035
1.639ThrTyr: 1.639 ± 0.064
0.0ThrXaa: 0.0 ± 0.0
Val
3.803ValAla: 3.803 ± 0.1
0.769ValCys: 0.769 ± 0.045
3.831ValAsp: 3.831 ± 0.089
4.102ValGlu: 4.102 ± 0.09
3.002ValPhe: 3.002 ± 0.1
4.291ValGly: 4.291 ± 0.121
1.163ValHis: 1.163 ± 0.048
5.804ValIle: 5.804 ± 0.112
5.031ValLys: 5.031 ± 0.1
5.525ValLeu: 5.525 ± 0.104
1.651ValMet: 1.651 ± 0.064
2.881ValAsn: 2.881 ± 0.095
2.212ValPro: 2.212 ± 0.068
1.643ValGln: 1.643 ± 0.065
2.254ValArg: 2.254 ± 0.076
4.777ValSer: 4.777 ± 0.105
3.716ValThr: 3.716 ± 0.097
4.157ValVal: 4.157 ± 0.121
0.567ValTrp: 0.567 ± 0.038
1.852ValTyr: 1.852 ± 0.064
0.0ValXaa: 0.0 ± 0.0
Trp
0.554TrpAla: 0.554 ± 0.032
0.092TrpCys: 0.092 ± 0.014
0.482TrpAsp: 0.482 ± 0.042
0.52TrpGlu: 0.52 ± 0.033
0.46TrpPhe: 0.46 ± 0.036
0.537TrpGly: 0.537 ± 0.037
0.217TrpHis: 0.217 ± 0.023
0.861TrpIle: 0.861 ± 0.051
0.763TrpLys: 0.763 ± 0.043
0.767TrpLeu: 0.767 ± 0.041
0.317TrpMet: 0.317 ± 0.027
0.641TrpAsn: 0.641 ± 0.039
0.266TrpPro: 0.266 ± 0.024
0.324TrpGln: 0.324 ± 0.027
0.377TrpArg: 0.377 ± 0.03
0.556TrpSer: 0.556 ± 0.034
0.486TrpThr: 0.486 ± 0.037
0.605TrpVal: 0.605 ± 0.033
0.17TrpTrp: 0.17 ± 0.022
0.322TrpTyr: 0.322 ± 0.028
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.86TyrAla: 1.86 ± 0.068
0.471TyrCys: 0.471 ± 0.029
2.395TyrAsp: 2.395 ± 0.076
1.952TyrGlu: 1.952 ± 0.068
1.528TyrPhe: 1.528 ± 0.064
2.214TyrGly: 2.214 ± 0.07
0.673TyrHis: 0.673 ± 0.041
1.798TyrIle: 1.798 ± 0.056
2.028TyrLys: 2.028 ± 0.065
2.891TyrLeu: 2.891 ± 0.088
0.716TyrMet: 0.716 ± 0.038
1.43TyrAsn: 1.43 ± 0.056
1.27TyrPro: 1.27 ± 0.057
1.11TyrGln: 1.11 ± 0.051
1.244TyrArg: 1.244 ± 0.055
2.365TyrSer: 2.365 ± 0.072
1.502TyrThr: 1.502 ± 0.055
2.116TyrVal: 2.116 ± 0.069
0.362TyrTrp: 0.362 ± 0.027
1.172TyrTyr: 1.172 ± 0.058
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.002XaaGly: 0.002 ± 0.002
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.002XaaLeu: 0.002 ± 0.002
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.002XaaArg: 0.002 ± 0.002
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2154 proteins (469320 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski