Streptomyces collinus (strain DSM 40733 / Tue 365)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; Streptomyces collinus

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6682 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5VDM8|S5VDM8_STRC3 Cupin_2 domain-containing protein OS=Streptomyces collinus (strain DSM 40733 / Tue 365) OX=1214242 GN=B446_09040 PE=4 SV=1
MM1 pKa = 7.41TVQQEE6 pKa = 4.49APGGEE11 pKa = 4.07ALEE14 pKa = 4.15VWIDD18 pKa = 3.41QDD20 pKa = 3.99LCTGDD25 pKa = 5.01GICAQYY31 pKa = 10.9APEE34 pKa = 4.23VFEE37 pKa = 5.9LDD39 pKa = 3.05IDD41 pKa = 3.65GLAYY45 pKa = 10.73VKK47 pKa = 10.8GADD50 pKa = 4.51DD51 pKa = 5.31ALLQAAGATTPVPLPLLTDD70 pKa = 3.69VVDD73 pKa = 4.29SARR76 pKa = 11.84EE77 pKa = 3.94CPGEE81 pKa = 4.38CIHH84 pKa = 6.24VRR86 pKa = 11.84RR87 pKa = 11.84VSDD90 pKa = 3.3RR91 pKa = 11.84VEE93 pKa = 4.16VYY95 pKa = 10.89GPDD98 pKa = 3.45AEE100 pKa = 4.31

Molecular weight: 10.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5UY92|S5UY92_STRC3 Hydrolase OS=Streptomyces collinus (strain DSM 40733 / Tue 365) OX=1214242 GN=B446_05640 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.89GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6682

0

6682

2231205

31

6643

333.9

35.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.951 ± 0.054

0.801 ± 0.008

5.991 ± 0.026

5.575 ± 0.032

2.687 ± 0.018

9.688 ± 0.031

2.388 ± 0.015

2.844 ± 0.025

2.037 ± 0.027

10.437 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.644 ± 0.014

1.642 ± 0.019

6.256 ± 0.032

2.673 ± 0.019

8.346 ± 0.038

4.803 ± 0.021

6.106 ± 0.028

8.576 ± 0.03

1.497 ± 0.011

2.058 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski