Circoviridae 4 LDMD-2013

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; environmental samples; Circoviridae 17 LDMD-2013

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5TNB8|S5TNB8_9CIRC ATP-dependent helicase Rep OS=Circoviridae 4 LDMD-2013 OX=1379708 PE=3 SV=1
MM1 pKa = 7.61SSSDD5 pKa = 3.69YY6 pKa = 11.68SNDD9 pKa = 2.9IDD11 pKa = 5.72DD12 pKa = 3.75EE13 pKa = 5.38HH14 pKa = 6.61EE15 pKa = 4.59LKK17 pKa = 10.96FLDD20 pKa = 3.82TTVLDD25 pKa = 4.04SVVATTGSLTEE36 pKa = 4.21LSLVAEE42 pKa = 5.08GVSEE46 pKa = 4.13SQRR49 pKa = 11.84VGRR52 pKa = 11.84RR53 pKa = 11.84IVATSVDD60 pKa = 4.6FIGNAYY66 pKa = 9.94LPAVTSLEE74 pKa = 4.05VTPEE78 pKa = 4.26GEE80 pKa = 4.47CLRR83 pKa = 11.84VMVLLDD89 pKa = 3.31HH90 pKa = 6.27QANGFATAVSEE101 pKa = 4.28ILPGTDD107 pKa = 2.49INNHH111 pKa = 5.19IRR113 pKa = 11.84RR114 pKa = 11.84TDD116 pKa = 3.05QRR118 pKa = 11.84RR119 pKa = 11.84FQILGEE125 pKa = 4.05RR126 pKa = 11.84FFDD129 pKa = 4.61LRR131 pKa = 11.84YY132 pKa = 10.25CGLTFSDD139 pKa = 3.53TGTGYY144 pKa = 10.65DD145 pKa = 3.64YY146 pKa = 11.23PGVANQFHH154 pKa = 5.33WHH156 pKa = 5.67VPIEE160 pKa = 4.17SPIYY164 pKa = 9.44YY165 pKa = 9.95EE166 pKa = 4.34EE167 pKa = 5.18GNTRR171 pKa = 11.84PVTGNLLLLLISKK184 pKa = 10.07IGTAGFEE191 pKa = 3.8AEE193 pKa = 4.28ARR195 pKa = 11.84FNYY198 pKa = 8.92TDD200 pKa = 3.06NN201 pKa = 3.88

Molecular weight: 22.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5TMU7|S5TMU7_9CIRC Coat protein OS=Circoviridae 4 LDMD-2013 OX=1379708 PE=4 SV=1
MM1 pKa = 7.69PGYY4 pKa = 10.28NRR6 pKa = 11.84RR7 pKa = 11.84SQFRR11 pKa = 11.84SQSSSRR17 pKa = 11.84PSKK20 pKa = 9.96RR21 pKa = 11.84WAEE24 pKa = 4.12SRR26 pKa = 11.84ASGARR31 pKa = 11.84SQAVFTARR39 pKa = 11.84ARR41 pKa = 11.84TAMARR46 pKa = 11.84GRR48 pKa = 11.84FAGHH52 pKa = 5.34YY53 pKa = 8.41RR54 pKa = 11.84KK55 pKa = 9.88SGYY58 pKa = 8.79YY59 pKa = 10.15GRR61 pKa = 11.84YY62 pKa = 9.25NRR64 pKa = 11.84DD65 pKa = 2.72TGTEE69 pKa = 4.14LKK71 pKa = 10.78FLDD74 pKa = 4.15TSIDD78 pKa = 3.64DD79 pKa = 5.33AIVSTTLTVQTAGPNLIPQGTAEE102 pKa = 4.36DD103 pKa = 3.86EE104 pKa = 4.16RR105 pKa = 11.84VGRR108 pKa = 11.84KK109 pKa = 8.07VVVKK113 pKa = 10.23SIHH116 pKa = 4.14WRR118 pKa = 11.84YY119 pKa = 10.07RR120 pKa = 11.84CLLPSSTSQNDD131 pKa = 3.23TSEE134 pKa = 4.51VIRR137 pKa = 11.84VMLLLDD143 pKa = 3.67KK144 pKa = 10.51QCNGSYY150 pKa = 10.21PGASEE155 pKa = 4.6ILQTNNDD162 pKa = 3.24WQSFNNLSNKK172 pKa = 9.73DD173 pKa = 3.78RR174 pKa = 11.84FVTLYY179 pKa = 10.95DD180 pKa = 3.17KK181 pKa = 10.93TIALNSMGNGGNGTTNEE198 pKa = 4.22SNEE201 pKa = 3.75VRR203 pKa = 11.84HH204 pKa = 6.19CEE206 pKa = 3.99EE207 pKa = 3.39FHH209 pKa = 6.84KK210 pKa = 10.81KK211 pKa = 9.51VNIPINFDD219 pKa = 3.47STTGAVTEE227 pKa = 4.14IASNNLVVALISEE240 pKa = 4.68GGLPVFNSNMRR251 pKa = 11.84IRR253 pKa = 11.84FEE255 pKa = 4.07GG256 pKa = 3.41

Molecular weight: 28.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

850

201

393

283.3

31.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.059 ± 0.48

1.294 ± 0.134

6.824 ± 1.08

5.882 ± 0.567

3.529 ± 0.592

8.941 ± 0.687

2.353 ± 0.331

3.882 ± 0.757

3.294 ± 0.786

7.294 ± 0.921

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.412 ± 0.236

4.588 ± 1.616

6.353 ± 1.981

4.118 ± 0.712

7.059 ± 0.804

7.529 ± 1.301

7.294 ± 0.701

6.471 ± 0.514

1.647 ± 0.516

3.176 ± 0.283
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski