Aeromicrobium sp. PE09-221

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Aeromicrobium; unclassified Aeromicrobium

Average proteome isoelectric point is 5.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3780 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A200H9M9|A0A200H9M9_9ACTN Anti-sigma factor antagonist OS=Aeromicrobium sp. PE09-221 OX=1898043 GN=BHE97_10925 PE=3 SV=1
MM1 pKa = 7.12NRR3 pKa = 11.84SAAIVLGAAGIVLALIVAAAVYY25 pKa = 10.35VIGDD29 pKa = 3.56WDD31 pKa = 4.2GDD33 pKa = 4.02DD34 pKa = 4.3PAQSATPTGEE44 pKa = 3.96QAPEE48 pKa = 4.07GLDD51 pKa = 3.17AFYY54 pKa = 10.73DD55 pKa = 3.74QQIDD59 pKa = 3.67WEE61 pKa = 4.62SCDD64 pKa = 4.74SGDD67 pKa = 4.45ARR69 pKa = 11.84CATAEE74 pKa = 4.17VPVDD78 pKa = 3.51YY79 pKa = 10.36DD80 pKa = 3.83DD81 pKa = 5.51PSGEE85 pKa = 4.37TIDD88 pKa = 4.61LALLKK93 pKa = 10.78VEE95 pKa = 4.48ATGEE99 pKa = 4.03RR100 pKa = 11.84QGTIFVNPGGPGGSAQDD117 pKa = 3.55FALMMGARR125 pKa = 11.84FPSSVRR131 pKa = 11.84EE132 pKa = 4.06AYY134 pKa = 10.3DD135 pKa = 3.19IVGMDD140 pKa = 3.48PRR142 pKa = 11.84GVGDD146 pKa = 3.98STPLQCLDD154 pKa = 4.62DD155 pKa = 5.02ADD157 pKa = 4.06FDD159 pKa = 4.69AFIALDD165 pKa = 4.36PDD167 pKa = 4.05PEE169 pKa = 4.37TPEE172 pKa = 4.25EE173 pKa = 4.13IEE175 pKa = 3.99EE176 pKa = 4.09SRR178 pKa = 11.84EE179 pKa = 3.97GIEE182 pKa = 5.7AMGEE186 pKa = 3.97ACRR189 pKa = 11.84EE190 pKa = 4.01NSGDD194 pKa = 3.61LAAHH198 pKa = 6.42VSTAEE203 pKa = 3.89VARR206 pKa = 11.84DD207 pKa = 3.45HH208 pKa = 7.15DD209 pKa = 4.35VVRR212 pKa = 11.84AALGEE217 pKa = 4.18EE218 pKa = 4.07TFNWFGASYY227 pKa = 8.11GTQLGAQYY235 pKa = 11.64AEE237 pKa = 4.2MFPDD241 pKa = 3.01RR242 pKa = 11.84VGRR245 pKa = 11.84MVLDD249 pKa = 4.38GGLDD253 pKa = 3.54PALSSIEE260 pKa = 4.0VSLGQAEE267 pKa = 4.73GFQRR271 pKa = 11.84ALDD274 pKa = 4.21AYY276 pKa = 9.84IADD279 pKa = 4.38CVSSGDD285 pKa = 3.98CPLGSSEE292 pKa = 6.05DD293 pKa = 3.76EE294 pKa = 4.49ALDD297 pKa = 3.67AVAGFMADD305 pKa = 4.25LEE307 pKa = 4.29QEE309 pKa = 4.28PLVSRR314 pKa = 11.84GGRR317 pKa = 11.84EE318 pKa = 3.96LTEE321 pKa = 3.89GLAFYY326 pKa = 10.55GVAVTLYY333 pKa = 10.7AKK335 pKa = 10.15EE336 pKa = 3.91SWSYY340 pKa = 9.45LTAAFEE346 pKa = 4.52AALSDD351 pKa = 4.16DD352 pKa = 4.2DD353 pKa = 5.22PGVFLQLADD362 pKa = 3.37IYY364 pKa = 10.91FDD366 pKa = 3.61RR367 pKa = 11.84NARR370 pKa = 11.84GEE372 pKa = 4.05FQDD375 pKa = 4.02NSGQAIYY382 pKa = 10.49AVNCLDD388 pKa = 3.94GPLDD392 pKa = 3.59VTVEE396 pKa = 4.21EE397 pKa = 5.43VEE399 pKa = 4.89SEE401 pKa = 3.67WLPRR405 pKa = 11.84FRR407 pKa = 11.84EE408 pKa = 4.02ASPVFGGALGWGVMACADD426 pKa = 3.75WPIEE430 pKa = 4.09STNPQEE436 pKa = 3.96PVAAEE441 pKa = 3.98GADD444 pKa = 3.78PIVVVGTTRR453 pKa = 11.84DD454 pKa = 3.22PATPYY459 pKa = 10.18EE460 pKa = 4.31WSQSLAEE467 pKa = 4.21EE468 pKa = 4.47LSSGVLVTRR477 pKa = 11.84EE478 pKa = 4.13GDD480 pKa = 3.19GHH482 pKa = 6.18TGYY485 pKa = 11.73GMGNACVDD493 pKa = 3.77EE494 pKa = 5.39AINAYY499 pKa = 9.79LVEE502 pKa = 4.29GTVPDD507 pKa = 4.98DD508 pKa = 4.14GLVCAEE514 pKa = 4.17

Molecular weight: 54.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A200HK11|A0A200HK11_9ACTN ABC transmembrane type-1 domain-containing protein OS=Aeromicrobium sp. PE09-221 OX=1898043 GN=BHE97_00320 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.97KK16 pKa = 9.33HH17 pKa = 4.25GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILSSRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.74GRR40 pKa = 11.84ANLSAA45 pKa = 4.66

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3780

0

3780

1179454

29

3016

312.0

33.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.627 ± 0.056

0.659 ± 0.01

6.49 ± 0.034

6.278 ± 0.041

2.928 ± 0.022

8.969 ± 0.037

2.169 ± 0.02

4.456 ± 0.027

1.813 ± 0.03

10.146 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.942 ± 0.017

1.784 ± 0.022

5.282 ± 0.025

2.822 ± 0.022

7.677 ± 0.047

5.534 ± 0.028

6.015 ± 0.029

8.988 ± 0.037

1.493 ± 0.017

1.926 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski