Deltaproteobacteria bacterium
Average proteome isoelectric point is 7.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2307 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A318CLF1|A0A318CLF1_9DELT Uncharacterized protein OS=Deltaproteobacteria bacterium OX=2026735 GN=C4B57_08675 PE=4 SV=1
MM1 pKa = 7.2 GRR3 pKa = 11.84 MVMYY7 pKa = 9.78 FKK9 pKa = 10.43 TLRR12 pKa = 11.84 ALLLGLCIVSMAAVSWVSSSLAEE35 pKa = 4.13 EE36 pKa = 4.98 GNLEE40 pKa = 4.1 QQVQEE45 pKa = 5.14 LIQQNRR51 pKa = 11.84 ALTDD55 pKa = 3.07 RR56 pKa = 11.84 LTEE59 pKa = 4.03 VEE61 pKa = 4.11 QQLNEE66 pKa = 4.09 MKK68 pKa = 10.48 AVPEE72 pKa = 4.04 PSRR75 pKa = 11.84 AEE77 pKa = 3.96 GVGFLSDD84 pKa = 4.34 LEE86 pKa = 4.12 DD87 pKa = 4.98 HH88 pKa = 6.22 ITINGLLEE96 pKa = 4.15 FGAAYY101 pKa = 10.31 HH102 pKa = 5.56 STDD105 pKa = 3.3 MNHH108 pKa = 6.55 SGFEE112 pKa = 4.03 HH113 pKa = 7.4 DD114 pKa = 4.89 SDD116 pKa = 3.67 ISMTSVEE123 pKa = 4.53 LGIEE127 pKa = 4.01 AEE129 pKa = 4.11 INDD132 pKa = 4.0 WVSAEE137 pKa = 4.13 MILLFEE143 pKa = 5.35 DD144 pKa = 3.82 PSGAINTYY152 pKa = 10.09 EE153 pKa = 5.3 DD154 pKa = 3.45 IRR156 pKa = 11.84 DD157 pKa = 3.79 DD158 pKa = 3.58 EE159 pKa = 4.92 TNFDD163 pKa = 3.68 VEE165 pKa = 4.37 EE166 pKa = 4.19 AFITIANPDD175 pKa = 3.54 VTPGFLRR182 pKa = 11.84 AGKK185 pKa = 9.34 MYY187 pKa = 10.82 VPFGALLTHH196 pKa = 6.91 FPDD199 pKa = 5.37 DD200 pKa = 3.87 MGIDD204 pKa = 3.67 SPLTLLLGEE213 pKa = 4.4 TLEE216 pKa = 4.28 KK217 pKa = 10.87 ALVIGAEE224 pKa = 4.12 YY225 pKa = 10.91 AGFTVSAYY233 pKa = 10.37 VFNGDD238 pKa = 3.19 VEE240 pKa = 4.42 EE241 pKa = 5.41 AGGGDD246 pKa = 3.86 NVIEE250 pKa = 4.49 TYY252 pKa = 11.07 GFDD255 pKa = 3.36 ANYY258 pKa = 10.8 AFEE261 pKa = 6.34 DD262 pKa = 3.89 EE263 pKa = 5.61 DD264 pKa = 4.96 LGLDD268 pKa = 3.72 LLVGGSYY275 pKa = 10.32 ISNIADD281 pKa = 3.46 SDD283 pKa = 3.63 WTEE286 pKa = 3.92 EE287 pKa = 4.15 VLDD290 pKa = 4.78 HH291 pKa = 7.05 YY292 pKa = 11.35 DD293 pKa = 5.46 LEE295 pKa = 6.06 IDD297 pKa = 4.84 DD298 pKa = 4.4 YY299 pKa = 12.13 AEE301 pKa = 4.0 GAAAYY306 pKa = 9.7 LHH308 pKa = 6.43 VGYY311 pKa = 10.53 QGLFVEE317 pKa = 5.13 AEE319 pKa = 3.77 YY320 pKa = 9.53 MAAVDD325 pKa = 3.71 HH326 pKa = 6.47 VNVEE330 pKa = 4.32 GEE332 pKa = 4.42 DD333 pKa = 3.55 LGKK336 pKa = 8.53 PTVWNIEE343 pKa = 3.38 AGYY346 pKa = 10.38 NYY348 pKa = 10.44 NWWRR352 pKa = 11.84 NLEE355 pKa = 3.87 IALQYY360 pKa = 10.93 AGSDD364 pKa = 3.49 DD365 pKa = 4.3 CGSLRR370 pKa = 11.84 LPRR373 pKa = 11.84 DD374 pKa = 2.93 RR375 pKa = 11.84 YY376 pKa = 10.71 GINFNQEE383 pKa = 3.4 IFDD386 pKa = 3.83 NTILSVGYY394 pKa = 9.21 MYY396 pKa = 10.9 DD397 pKa = 3.45 EE398 pKa = 4.8 YY399 pKa = 11.39 ATDD402 pKa = 5.42 DD403 pKa = 4.04 DD404 pKa = 5.02 LDD406 pKa = 4.66 RR407 pKa = 11.84 NTRR410 pKa = 11.84 DD411 pKa = 3.93 LIFGQLAIEE420 pKa = 4.51 FF421 pKa = 4.31
Molecular weight: 46.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.725
IPC2_protein 3.77
IPC_protein 3.783
Toseland 3.579
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.668
Rodwell 3.605
Grimsley 3.478
Solomon 3.757
Lehninger 3.706
Nozaki 3.872
DTASelect 4.062
Thurlkill 3.617
EMBOSS 3.681
Sillero 3.897
Patrickios 0.82
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.805
Protein with the highest isoelectric point:
>tr|A0A318CJK4|A0A318CJK4_9DELT t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaE OS=Deltaproteobacteria bacterium OX=2026735 GN=C4B57_09540 PE=3 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPNNLKK11 pKa = 10.39 RR12 pKa = 11.84 KK13 pKa = 7.14 RR14 pKa = 11.84 THH16 pKa = 6.05 GFRR19 pKa = 11.84 KK20 pKa = 9.99 RR21 pKa = 11.84 MSTKK25 pKa = 9.76 AGRR28 pKa = 11.84 RR29 pKa = 11.84 ILNRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.26 GRR39 pKa = 11.84 HH40 pKa = 5.14 SLSAA44 pKa = 3.67
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.705
Grimsley 13.042
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.427
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2307
0
2307
678707
35
1733
294.2
32.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.881 ± 0.046
1.389 ± 0.027
5.45 ± 0.037
6.893 ± 0.054
4.101 ± 0.031
7.618 ± 0.051
2.16 ± 0.024
7.002 ± 0.039
5.883 ± 0.052
10.326 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.473 ± 0.025
3.204 ± 0.026
4.571 ± 0.036
3.009 ± 0.025
6.649 ± 0.048
5.843 ± 0.035
4.627 ± 0.028
6.747 ± 0.04
1.171 ± 0.022
3.002 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here