Amino acid dipepetide frequency for Deltaproteobacteria bacterium

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.974AlaAla: 6.974 ± 0.131	1.273AlaCys: 1.273 ± 0.049	4.016AlaAsp: 4.016 ± 0.081	5.334AlaGlu: 5.334 ± 0.092	3.321AlaPhe: 3.321 ± 0.08	7.195AlaGly: 7.195 ± 0.134	1.519AlaHis: 1.519 ± 0.046	5.204AlaIle: 5.204 ± 0.088	4.022AlaLys: 4.022 ± 0.089	8.603AlaLeu: 8.603 ± 0.125	2.293AlaMet: 2.293 ± 0.064	2.032AlaAsn: 2.032 ± 0.055	2.583AlaPro: 2.583 ± 0.067	2.237AlaGln: 2.237 ± 0.059	5.54AlaArg: 5.54 ± 0.102	4.192AlaSer: 4.192 ± 0.078	3.222AlaThr: 3.222 ± 0.071	6.132AlaVal: 6.132 ± 0.103	0.934AlaTrp: 0.934 ± 0.04	2.184AlaTyr: 2.184 ± 0.062	0.0AlaXaa: 0.0 ± 0.0
Cys	0.916CysAla: 0.916 ± 0.043	0.23CysCys: 0.23 ± 0.02	0.614CysAsp: 0.614 ± 0.036	0.647CysGlu: 0.647 ± 0.034	0.579CysPhe: 0.579 ± 0.03	1.353CysGly: 1.353 ± 0.054	0.641CysHis: 0.641 ± 0.071	0.86CysIle: 0.86 ± 0.039	0.631CysLys: 0.631 ± 0.03	1.554CysLeu: 1.554 ± 0.049	0.333CysMet: 0.333 ± 0.022	0.449CysAsn: 0.449 ± 0.028	0.93CysPro: 0.93 ± 0.04	0.386CysGln: 0.386 ± 0.023	1.036CysArg: 1.036 ± 0.043	0.804CysSer: 0.804 ± 0.043	0.62CysThr: 0.62 ± 0.026	0.709CysVal: 0.709 ± 0.037	0.158CysTrp: 0.158 ± 0.017	0.439CysTyr: 0.439 ± 0.024	0.0CysXaa: 0.0 ± 0.0
Asp	3.619AspAla: 3.619 ± 0.077	0.647AspCys: 0.647 ± 0.033	2.487AspAsp: 2.487 ± 0.069	3.518AspGlu: 3.518 ± 0.081	2.629AspPhe: 2.629 ± 0.06	3.513AspGly: 3.513 ± 0.086	1.26AspHis: 1.26 ± 0.037	4.598AspIle: 4.598 ± 0.085	2.826AspLys: 2.826 ± 0.062	6.623AspLeu: 6.623 ± 0.105	1.451AspMet: 1.451 ± 0.044	1.519AspAsn: 1.519 ± 0.048	3.022AspPro: 3.022 ± 0.064	1.625AspGln: 1.625 ± 0.046	3.906AspArg: 3.906 ± 0.084	2.67AspSer: 2.67 ± 0.065	2.502AspThr: 2.502 ± 0.065	3.474AspVal: 3.474 ± 0.065	0.723AspTrp: 0.723 ± 0.032	1.884AspTyr: 1.884 ± 0.059	0.0AspXaa: 0.0 ± 0.0
Glu	5.869GluAla: 5.869 ± 0.105	0.653GluCys: 0.653 ± 0.031	3.701GluAsp: 3.701 ± 0.069	5.295GluGlu: 5.295 ± 0.098	2.298GluPhe: 2.298 ± 0.054	4.591GluGly: 4.591 ± 0.081	1.342GluHis: 1.342 ± 0.047	5.854GluIle: 5.854 ± 0.097	5.254GluLys: 5.254 ± 0.127	6.44GluLeu: 6.44 ± 0.112	1.753GluMet: 1.753 ± 0.052	2.413GluAsn: 2.413 ± 0.064	2.374GluPro: 2.374 ± 0.057	2.265GluGln: 2.265 ± 0.06	4.731GluArg: 4.731 ± 0.095	3.274GluSer: 3.274 ± 0.076	3.37GluThr: 3.37 ± 0.078	4.902GluVal: 4.902 ± 0.088	0.688GluTrp: 0.688 ± 0.035	1.865GluTyr: 1.865 ± 0.057	0.0GluXaa: 0.0 ± 0.0
Phe	2.792PheAla: 2.792 ± 0.066	0.735PheCys: 0.735 ± 0.036	2.303PheAsp: 2.303 ± 0.055	2.537PheGlu: 2.537 ± 0.061	2.098PhePhe: 2.098 ± 0.064	2.948PheGly: 2.948 ± 0.061	0.891PheHis: 0.891 ± 0.035	2.595PheIle: 2.595 ± 0.059	1.927PheLys: 1.927 ± 0.056	4.566PheLeu: 4.566 ± 0.096	0.996PheMet: 0.996 ± 0.045	1.372PheAsn: 1.372 ± 0.044	1.889PhePro: 1.889 ± 0.056	1.325PheGln: 1.325 ± 0.046	2.58PheArg: 2.58 ± 0.074	3.065PheSer: 3.065 ± 0.066	1.989PheThr: 1.989 ± 0.057	2.496PheVal: 2.496 ± 0.068	0.567PheTrp: 0.567 ± 0.03	1.339PheTyr: 1.339 ± 0.05	0.0PheXaa: 0.0 ± 0.0
Gly	5.232GlyAla: 5.232 ± 0.1	1.155GlyCys: 1.155 ± 0.048	3.636GlyAsp: 3.636 ± 0.077	4.587GlyGlu: 4.587 ± 0.089	3.206GlyPhe: 3.206 ± 0.079	5.236GlyGly: 5.236 ± 0.102	1.837GlyHis: 1.837 ± 0.053	5.833GlyIle: 5.833 ± 0.102	5.077GlyLys: 5.077 ± 0.108	8.54GlyLeu: 8.54 ± 0.127	2.038GlyMet: 2.038 ± 0.058	2.489GlyAsn: 2.489 ± 0.058	3.037GlyPro: 3.037 ± 0.071	2.306GlyGln: 2.306 ± 0.061	5.334GlyArg: 5.334 ± 0.106	4.282GlySer: 4.282 ± 0.082	3.971GlyThr: 3.971 ± 0.073	4.836GlyVal: 4.836 ± 0.106	0.958GlyTrp: 0.958 ± 0.039	2.593GlyTyr: 2.593 ± 0.064	0.0GlyXaa: 0.0 ± 0.0
His	1.488HisAla: 1.488 ± 0.053	0.388HisCys: 0.388 ± 0.026	1.005HisAsp: 1.005 ± 0.037	1.313HisGlu: 1.313 ± 0.049	0.927HisPhe: 0.927 ± 0.035	1.809HisGly: 1.809 ± 0.054	0.536HisHis: 0.536 ± 0.03	1.494HisIle: 1.494 ± 0.046	1.055HisLys: 1.055 ± 0.039	2.428HisLeu: 2.428 ± 0.071	0.497HisMet: 0.497 ± 0.029	0.704HisAsn: 0.704 ± 0.036	1.401HisPro: 1.401 ± 0.043	0.669HisGln: 0.669 ± 0.041	1.478HisArg: 1.478 ± 0.048	1.143HisSer: 1.143 ± 0.046	0.964HisThr: 0.964 ± 0.036	1.313HisVal: 1.313 ± 0.045	0.276HisTrp: 0.276 ± 0.02	0.715HisTyr: 0.715 ± 0.035	0.0HisXaa: 0.0 ± 0.0
Ile	5.086IleAla: 5.086 ± 0.1	1.015IleCys: 1.015 ± 0.04	3.963IleAsp: 3.963 ± 0.069	4.831IleGlu: 4.831 ± 0.088	2.873IlePhe: 2.873 ± 0.061	4.815IleGly: 4.815 ± 0.089	1.51IleHis: 1.51 ± 0.047	4.584IleIle: 4.584 ± 0.099	4.236IleLys: 4.236 ± 0.087	7.308IleLeu: 7.308 ± 0.106	1.498IleMet: 1.498 ± 0.046	2.496IleAsn: 2.496 ± 0.074	3.514IlePro: 3.514 ± 0.081	2.097IleGln: 2.097 ± 0.059	4.775IleArg: 4.775 ± 0.094	4.924IleSer: 4.924 ± 0.083	3.455IleThr: 3.455 ± 0.072	4.321IleVal: 4.321 ± 0.088	0.632IleTrp: 0.632 ± 0.033	2.082IleTyr: 2.082 ± 0.065	0.0IleXaa: 0.0 ± 0.0
Lys	5.359LysAla: 5.359 ± 0.101	0.597LysCys: 0.597 ± 0.032	3.642LysAsp: 3.642 ± 0.081	5.145LysGlu: 5.145 ± 0.107	1.493LysPhe: 1.493 ± 0.048	4.756LysGly: 4.756 ± 0.092	0.906LysHis: 0.906 ± 0.038	4.28LysIle: 4.28 ± 0.093	4.596LysLys: 4.596 ± 0.104	4.397LysLeu: 4.397 ± 0.088	1.465LysMet: 1.465 ± 0.051	2.259LysAsn: 2.259 ± 0.061	2.278LysPro: 2.278 ± 0.059	1.684LysGln: 1.684 ± 0.059	3.735LysArg: 3.735 ± 0.079	2.963LysSer: 2.963 ± 0.071	3.103LysThr: 3.103 ± 0.082	3.891LysVal: 3.891 ± 0.086	0.573LysTrp: 0.573 ± 0.028	1.705LysTyr: 1.705 ± 0.057	0.0LysXaa: 0.0 ± 0.0
Leu	9.175LeuAla: 9.175 ± 0.136	1.361LeuCys: 1.361 ± 0.048	6.081LeuAsp: 6.081 ± 0.103	7.208LeuGlu: 7.208 ± 0.119	4.19LeuPhe: 4.19 ± 0.091	7.482LeuGly: 7.482 ± 0.12	1.966LeuHis: 1.966 ± 0.051	6.412LeuIle: 6.412 ± 0.112	6.803LeuLys: 6.803 ± 0.118	10.15LeuLeu: 10.15 ± 0.172	2.347LeuMet: 2.347 ± 0.064	3.423LeuAsn: 3.423 ± 0.073	4.918LeuPro: 4.918 ± 0.084	2.847LeuGln: 2.847 ± 0.069	6.561LeuArg: 6.561 ± 0.123	7.224LeuSer: 7.224 ± 0.112	4.743LeuThr: 4.743 ± 0.082	6.448LeuVal: 6.448 ± 0.1	1.115LeuTrp: 1.115 ± 0.048	2.81LeuTyr: 2.81 ± 0.072	0.0LeuXaa: 0.0 ± 0.0
Met	2.699MetAla: 2.699 ± 0.063	0.249MetCys: 0.249 ± 0.019	1.528MetAsp: 1.528 ± 0.048	1.926MetGlu: 1.926 ± 0.055	0.774MetPhe: 0.774 ± 0.035	1.914MetGly: 1.914 ± 0.055	0.469MetHis: 0.469 ± 0.027	1.559MetIle: 1.559 ± 0.049	1.627MetLys: 1.627 ± 0.049	2.148MetLeu: 2.148 ± 0.064	0.541MetMet: 0.541 ± 0.03	0.888MetAsn: 0.888 ± 0.033	1.196MetPro: 1.196 ± 0.044	0.72MetGln: 0.72 ± 0.036	1.378MetArg: 1.378 ± 0.049	1.438MetSer: 1.438 ± 0.047	1.233MetThr: 1.233 ± 0.043	1.744MetVal: 1.744 ± 0.049	0.221MetTrp: 0.221 ± 0.02	0.479MetTyr: 0.479 ± 0.026	0.001MetXaa: 0.001 ± 0.001
Asn	2.346AsnAla: 2.346 ± 0.056	0.457AsnCys: 0.457 ± 0.026	1.45AsnAsp: 1.45 ± 0.051	1.852AsnGlu: 1.852 ± 0.058	1.32AsnPhe: 1.32 ± 0.046	2.141AsnGly: 2.141 ± 0.057	0.651AsnHis: 0.651 ± 0.032	2.749AsnIle: 2.749 ± 0.069	1.727AsnLys: 1.727 ± 0.054	3.417AsnLeu: 3.417 ± 0.072	0.896AsnMet: 0.896 ± 0.038	1.036AsnAsn: 1.036 ± 0.046	2.077AsnPro: 2.077 ± 0.06	0.984AsnGln: 0.984 ± 0.036	2.282AsnArg: 2.282 ± 0.058	1.64AsnSer: 1.64 ± 0.051	1.526AsnThr: 1.526 ± 0.052	2.044AsnVal: 2.044 ± 0.062	0.429AsnTrp: 0.429 ± 0.024	1.017AsnTyr: 1.017 ± 0.044	0.0AsnXaa: 0.0 ± 0.0
Pro	3.424ProAla: 3.424 ± 0.078	0.676ProCys: 0.676 ± 0.033	3.267ProAsp: 3.267 ± 0.075	4.316ProGlu: 4.316 ± 0.074	2.178ProPhe: 2.178 ± 0.05	4.174ProGly: 4.174 ± 0.102	1.014ProHis: 1.014 ± 0.043	2.338ProIle: 2.338 ± 0.058	2.042ProLys: 2.042 ± 0.052	4.274ProLeu: 4.274 ± 0.078	1.087ProMet: 1.087 ± 0.04	1.223ProAsn: 1.223 ± 0.041	1.827ProPro: 1.827 ± 0.062	1.261ProGln: 1.261 ± 0.042	2.301ProArg: 2.301 ± 0.054	2.543ProSer: 2.543 ± 0.061	1.585ProThr: 1.585 ± 0.043	4.139ProVal: 4.139 ± 0.076	0.642ProTrp: 0.642 ± 0.032	1.397ProTyr: 1.397 ± 0.044	0.0ProXaa: 0.0 ± 0.0
Gln	2.92GlnAla: 2.92 ± 0.075	0.355GlnCys: 0.355 ± 0.026	1.717GlnAsp: 1.717 ± 0.051	2.344GlnGlu: 2.344 ± 0.063	0.964GlnPhe: 0.964 ± 0.034	2.397GlnGly: 2.397 ± 0.057	0.535GlnHis: 0.535 ± 0.026	2.217GlnIle: 2.217 ± 0.051	2.194GlnLys: 2.194 ± 0.058	2.499GlnLeu: 2.499 ± 0.058	0.774GlnMet: 0.774 ± 0.036	0.977GlnAsn: 0.977 ± 0.039	1.092GlnPro: 1.092 ± 0.038	0.983GlnGln: 0.983 ± 0.041	1.918GlnArg: 1.918 ± 0.051	1.488GlnSer: 1.488 ± 0.047	1.311GlnThr: 1.311 ± 0.047	2.266GlnVal: 2.266 ± 0.056	0.352GlnTrp: 0.352 ± 0.026	0.79GlnTyr: 0.79 ± 0.038	0.0GlnXaa: 0.0 ± 0.0
Arg	4.495ArgAla: 4.495 ± 0.083	0.831ArgCys: 0.831 ± 0.042	3.526ArgAsp: 3.526 ± 0.081	4.662ArgGlu: 4.662 ± 0.091	2.951ArgPhe: 2.951 ± 0.072	4.133ArgGly: 4.133 ± 0.082	1.79ArgHis: 1.79 ± 0.052	5.036ArgIle: 5.036 ± 0.098	3.941ArgLys: 3.941 ± 0.079	7.584ArgLeu: 7.584 ± 0.119	1.674ArgMet: 1.674 ± 0.047	2.166ArgAsn: 2.166 ± 0.059	3.143ArgPro: 3.143 ± 0.069	2.391ArgGln: 2.391 ± 0.062	4.559ArgArg: 4.559 ± 0.111	3.711ArgSer: 3.711 ± 0.077	2.923ArgThr: 2.923 ± 0.069	3.934ArgVal: 3.934 ± 0.078	0.771ArgTrp: 0.771 ± 0.033	2.268ArgTyr: 2.268 ± 0.058	0.001ArgXaa: 0.001 ± 0.001
Ser	4.232SerAla: 4.232 ± 0.087	0.916SerCys: 0.916 ± 0.044	3.031SerAsp: 3.031 ± 0.065	3.726SerGlu: 3.726 ± 0.081	2.55SerPhe: 2.55 ± 0.059	5.403SerGly: 5.403 ± 0.108	1.269SerHis: 1.269 ± 0.045	3.844SerIle: 3.844 ± 0.072	2.827SerLys: 2.827 ± 0.063	6.309SerLeu: 6.309 ± 0.098	1.459SerMet: 1.459 ± 0.047	1.683SerAsn: 1.683 ± 0.049	2.91SerPro: 2.91 ± 0.064	1.758SerGln: 1.758 ± 0.042	4.22SerArg: 4.22 ± 0.086	3.599SerSer: 3.599 ± 0.075	2.34SerThr: 2.34 ± 0.06	3.801SerVal: 3.801 ± 0.078	0.772SerTrp: 0.772 ± 0.039	1.78SerTyr: 1.78 ± 0.054	0.0SerXaa: 0.0 ± 0.0
Thr	3.863ThrAla: 3.863 ± 0.072	0.672ThrCys: 0.672 ± 0.03	2.643ThrAsp: 2.643 ± 0.06	2.87ThrGlu: 2.87 ± 0.062	1.843ThrPhe: 1.843 ± 0.053	4.94ThrGly: 4.94 ± 0.084	0.946ThrHis: 0.946 ± 0.038	3.171ThrIle: 3.171 ± 0.067	2.045ThrLys: 2.045 ± 0.057	4.573ThrLeu: 4.573 ± 0.09	1.039ThrMet: 1.039 ± 0.041	1.252ThrAsn: 1.252 ± 0.044	2.282ThrPro: 2.282 ± 0.056	1.095ThrGln: 1.095 ± 0.045	2.799ThrArg: 2.799 ± 0.063	2.512ThrSer: 2.512 ± 0.062	2.179ThrThr: 2.179 ± 0.066	3.582ThrVal: 3.582 ± 0.078	0.542ThrTrp: 0.542 ± 0.029	1.423ThrTyr: 1.423 ± 0.045	0.0ThrXaa: 0.0 ± 0.0
Val	5.51ValAla: 5.51 ± 0.108	1.086ValCys: 1.086 ± 0.042	3.653ValAsp: 3.653 ± 0.078	4.227ValGlu: 4.227 ± 0.081	2.91ValPhe: 2.91 ± 0.066	4.189ValGly: 4.189 ± 0.093	1.479ValHis: 1.479 ± 0.055	4.814ValIle: 4.814 ± 0.076	3.782ValLys: 3.782 ± 0.072	7.174ValLeu: 7.174 ± 0.109	1.594ValMet: 1.594 ± 0.052	2.259ValAsn: 2.259 ± 0.065	3.088ValPro: 3.088 ± 0.068	1.884ValGln: 1.884 ± 0.059	4.444ValArg: 4.444 ± 0.086	4.467ValSer: 4.467 ± 0.083	3.383ValThr: 3.383 ± 0.07	4.912ValVal: 4.912 ± 0.103	0.743ValTrp: 0.743 ± 0.037	1.87ValTyr: 1.87 ± 0.047	0.0ValXaa: 0.0 ± 0.0
Trp	0.803TrpAla: 0.803 ± 0.032	0.127TrpCys: 0.127 ± 0.014	0.616TrpAsp: 0.616 ± 0.03	0.81TrpGlu: 0.81 ± 0.035	0.432TrpPhe: 0.432 ± 0.025	0.825TrpGly: 0.825 ± 0.036	0.309TrpHis: 0.309 ± 0.022	0.807TrpIle: 0.807 ± 0.038	0.69TrpLys: 0.69 ± 0.032	1.316TrpLeu: 1.316 ± 0.046	0.333TrpMet: 0.333 ± 0.023	0.385TrpAsn: 0.385 ± 0.026	0.555TrpPro: 0.555 ± 0.031	0.538TrpGln: 0.538 ± 0.031	0.738TrpArg: 0.738 ± 0.035	0.682TrpSer: 0.682 ± 0.032	0.494TrpThr: 0.494 ± 0.03	0.681TrpVal: 0.681 ± 0.035	0.23TrpTrp: 0.23 ± 0.018	0.342TrpTyr: 0.342 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.007TyrAla: 2.007 ± 0.059	0.467TyrCys: 0.467 ± 0.027	1.618TyrAsp: 1.618 ± 0.051	1.784TyrGlu: 1.784 ± 0.056	1.475TyrPhe: 1.475 ± 0.05	2.368TyrGly: 2.368 ± 0.063	0.729TyrHis: 0.729 ± 0.031	1.767TyrIle: 1.767 ± 0.058	1.523TyrLys: 1.523 ± 0.051	3.361TyrLeu: 3.361 ± 0.065	0.664TyrMet: 0.664 ± 0.035	1.017TyrAsn: 1.017 ± 0.04	1.591TyrPro: 1.591 ± 0.043	1.039TyrGln: 1.039 ± 0.042	2.175TyrArg: 2.175 ± 0.057	1.806TyrSer: 1.806 ± 0.054	1.36TyrThr: 1.36 ± 0.044	1.843TyrVal: 1.843 ± 0.05	0.386TyrTrp: 0.386 ± 0.025	1.039TyrTyr: 1.039 ± 0.048	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.001XaaHis: 0.001 ± 0.001	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.006XaaXaa: 0.006 ± 0.004

Statistics based on 2307 proteins (678707 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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