Desulfovibrio sp. 6_1_46AFAA
Average proteome isoelectric point is 6.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3312 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G1UPV3|G1UPV3_9DELT Uncharacterized protein OS=Desulfovibrio sp. 6_1_46AFAA OX=665942 GN=HMPREF1022_00626 PE=4 SV=1
MM1 pKa = 7.28 PTTHH5 pKa = 7.29 ILHH8 pKa = 6.75 LPAAGQKK15 pKa = 8.43 TVLAGFSPQSRR26 pKa = 11.84 MVLDD30 pKa = 4.44 FPTRR34 pKa = 11.84 GVFAGRR40 pKa = 11.84 EE41 pKa = 4.22 GNDD44 pKa = 3.33 LVFSRR49 pKa = 11.84 IDD51 pKa = 3.26 GTRR54 pKa = 11.84 LVLKK58 pKa = 10.12 DD59 pKa = 3.05 YY60 pKa = 9.81 HH61 pKa = 7.16 APQNVPEE68 pKa = 5.09 DD69 pKa = 3.98 VPQNAPADD77 pKa = 3.79 GAPRR81 pKa = 11.84 DD82 pKa = 4.04 EE83 pKa = 4.88 GPPAIVVDD91 pKa = 4.26 GRR93 pKa = 11.84 EE94 pKa = 3.99 MTHH97 pKa = 6.01 QAFFDD102 pKa = 3.77 ALGEE106 pKa = 4.22 DD107 pKa = 4.82 DD108 pKa = 4.59 MPQAGVVQARR118 pKa = 11.84 NSAYY122 pKa = 10.01 HH123 pKa = 6.73 EE124 pKa = 4.25 YY125 pKa = 10.72 SEE127 pKa = 5.16 SEE129 pKa = 3.93 LSKK132 pKa = 11.18 GISEE136 pKa = 5.03 LSALDD141 pKa = 3.6 DD142 pKa = 4.74 HH143 pKa = 6.14 EE144 pKa = 5.0 TEE146 pKa = 4.46 SDD148 pKa = 3.52 TNKK151 pKa = 10.2 PLGSRR156 pKa = 11.84 EE157 pKa = 3.61 GRR159 pKa = 11.84 QDD161 pKa = 3.2 DD162 pKa = 4.1 FAAEE166 pKa = 4.03 RR167 pKa = 11.84 GGVLGGTDD175 pKa = 3.4 TAGSGSNGSGSGSPGGSQGGNGGSGGSGHH204 pKa = 6.61 GGSGGTGGTGGNGSGGTGGTGNSNPSVASATVTIAEE240 pKa = 4.31 DD241 pKa = 3.98 TISVSGALPSPTDD254 pKa = 3.77 PNAGDD259 pKa = 3.42 ILSFIAKK266 pKa = 9.86 ANAAGLYY273 pKa = 7.61 GTLSVDD279 pKa = 3.08 ANGNYY284 pKa = 9.6 IYY286 pKa = 10.41 ILNNALSAVQGLGVGEE302 pKa = 4.65 SLTDD306 pKa = 3.28 TFTFTVTDD314 pKa = 3.67 NHH316 pKa = 6.75 GGTANNTLTVTITGTNDD333 pKa = 3.1 APVLAATTVNVSEE346 pKa = 5.08 DD347 pKa = 3.61 SNITSGILPTPTDD360 pKa = 3.24 VDD362 pKa = 3.68 VHH364 pKa = 6.46 DD365 pKa = 4.29 VVSFVPQTNTAGLYY379 pKa = 7.68 GTLSVDD385 pKa = 3.05 ANGNYY390 pKa = 9.81 TYY392 pKa = 11.26 VLNNTLPAVQGLGAGEE408 pKa = 4.41 NLTDD412 pKa = 3.65 TFTVTVTDD420 pKa = 3.52 NHH422 pKa = 6.7 GGTSSAVVTVNINGSNDD439 pKa = 3.23 APIVSAVATSIFEE452 pKa = 4.27 DD453 pKa = 3.79 TASVSGTLPAPVDD466 pKa = 3.8 PDD468 pKa = 3.47 LHH470 pKa = 7.15 DD471 pKa = 3.73 VASYY475 pKa = 10.86 LPQTNTVGLYY485 pKa = 7.99 GTLTLDD491 pKa = 3.08 ASGNYY496 pKa = 9.26 TYY498 pKa = 10.83 TLNNSLSAVQGLGVGEE514 pKa = 4.65 SLTDD518 pKa = 3.3 TFTFTASDD526 pKa = 3.55 GHH528 pKa = 6.58 GGTSSSTLTVTINGSNDD545 pKa = 3.3 APVLAATVVGVDD557 pKa = 3.58 KK558 pKa = 11.29 DD559 pKa = 4.21 DD560 pKa = 4.64 VITNGTLPTPTDD572 pKa = 3.22 VDD574 pKa = 3.63 VHH576 pKa = 6.17 DD577 pKa = 4.46 TVSFTPLTNAPGSYY591 pKa = 9.34 GTFSVDD597 pKa = 2.89 ANGNYY602 pKa = 9.77 TYY604 pKa = 11.3 VLNTSLPAVQGLGPADD620 pKa = 3.91 SLTDD624 pKa = 3.51 TFTVTVTDD632 pKa = 3.32 NHH634 pKa = 6.35 GASSSTVITVSINGSSNDD652 pKa = 3.5 APTVAAALASIGEE665 pKa = 4.4 DD666 pKa = 3.54 TPSVTGVLPTPGDD679 pKa = 4.15 PDD681 pKa = 3.45 PHH683 pKa = 7.65 DD684 pKa = 3.45 VVTFVPQAGTAGLYY698 pKa = 8.57 GTFSVDD704 pKa = 2.64 ASGNYY709 pKa = 9.32 TYY711 pKa = 10.74 TLNNSLPAVQGLGAGEE727 pKa = 4.47 SLTEE731 pKa = 4.05 TFTFTVSDD739 pKa = 3.8 GRR741 pKa = 11.84 GGTASNTLTVTIDD754 pKa = 3.61 GSNDD758 pKa = 3.22 APVVTAATASIVEE771 pKa = 4.26 DD772 pKa = 3.37 TAGISGTLPPAVDD785 pKa = 3.93 PDD787 pKa = 3.71 LHH789 pKa = 7.18 DD790 pKa = 3.6 VATFIPQTNTAGLYY804 pKa = 7.74 GTLTLDD810 pKa = 3.08 ASGNYY815 pKa = 9.26 TYY817 pKa = 10.74 TLNNSLPAVQGLGVGEE833 pKa = 4.56 SLTEE837 pKa = 4.12 TFTFTATDD845 pKa = 3.44 GHH847 pKa = 6.75 GGTSSNTLTVTINGSNDD864 pKa = 3.3 APVLAATTVNVAEE877 pKa = 5.0 DD878 pKa = 3.71 SAVTTGTLPMPTDD891 pKa = 3.4 VDD893 pKa = 3.73 VHH895 pKa = 6.18 DD896 pKa = 4.44 TVSFAPLTNAPGNYY910 pKa = 8.29 GTFSVDD916 pKa = 2.85 ANGNYY921 pKa = 9.77 TYY923 pKa = 11.3 VLNTSLPTVQGLGAGEE939 pKa = 4.61 SLTDD943 pKa = 3.48 SFTVTVSDD951 pKa = 3.4 NHH953 pKa = 6.57 GGSSSTVITVSINGSNDD970 pKa = 2.9 APTVSAAATAIDD982 pKa = 4.59 EE983 pKa = 4.53 DD984 pKa = 4.37 TASVSGTLPVPVDD997 pKa = 3.71 PDD999 pKa = 3.31 LHH1001 pKa = 7.22 DD1002 pKa = 3.67 VVSFVPQTNAPGGYY1016 pKa = 7.97 GTLSVDD1022 pKa = 2.91 ASGNYY1027 pKa = 9.32 TYY1029 pKa = 10.74 TLNNSLPAVQALGVGEE1045 pKa = 4.66 SLTDD1049 pKa = 3.28 TFTFTVTDD1057 pKa = 3.35 NHH1059 pKa = 6.79 GATTSNTLTVTINGTNDD1076 pKa = 3.28 LPTVSAAAASIAEE1089 pKa = 4.23 DD1090 pKa = 3.5 TAGISGMLPTPVDD1103 pKa = 3.4 VDD1105 pKa = 3.56 VHH1107 pKa = 5.85 DD1108 pKa = 4.34 TVTFTPQTNTAGLYY1122 pKa = 7.69 GTLNVDD1128 pKa = 2.84 ADD1130 pKa = 3.86 GNYY1133 pKa = 10.25 TYY1135 pKa = 10.45 TLNNASPAVQGLGVGEE1151 pKa = 4.51 NLTDD1155 pKa = 3.71 TFPFTVSDD1163 pKa = 3.69 GHH1165 pKa = 6.83 GGTATNTLTVTINGTNDD1182 pKa = 3.11 APIVAAATTAIAEE1195 pKa = 4.32 DD1196 pKa = 3.74 TAGVSGILPMPTDD1209 pKa = 3.43 VDD1211 pKa = 3.73 VHH1213 pKa = 6.17 DD1214 pKa = 4.49 TVSFTPQTNAPGSYY1228 pKa = 9.24 GTFSVDD1234 pKa = 3.01 ADD1236 pKa = 3.35 GHH1238 pKa = 4.49 YY1239 pKa = 9.84 TYY1241 pKa = 10.52 TLNNALPAVQGLGAGEE1257 pKa = 4.59 SLTDD1261 pKa = 3.26 TFTFTVSDD1269 pKa = 3.65 GRR1271 pKa = 11.84 GARR1274 pKa = 11.84 APIPP1278 pKa = 3.75
Molecular weight: 128.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.735
IPC2_protein 3.617
IPC_protein 3.694
Toseland 3.439
ProMoST 3.859
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.656
Rodwell 3.503
Grimsley 3.35
Solomon 3.694
Lehninger 3.656
Nozaki 3.795
DTASelect 4.113
Thurlkill 3.503
EMBOSS 3.656
Sillero 3.821
Patrickios 0.553
IPC_peptide 3.681
IPC2_peptide 3.783
IPC2.peptide.svr19 3.713
Protein with the highest isoelectric point:
>tr|G1UTI6|G1UTI6_9DELT GDP-mannose 4 6-dehydratase OS=Desulfovibrio sp. 6_1_46AFAA OX=665942 GN=gmd PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.07 QPSKK9 pKa = 9.07 IRR11 pKa = 11.84 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.96 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATPSGRR28 pKa = 11.84 AILRR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.08 GRR39 pKa = 11.84 KK40 pKa = 8.57 NLSAA44 pKa = 4.67
Molecular weight: 5.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.34
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.076
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.1
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3312
0
3312
1086973
22
4049
328.2
35.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.522 ± 0.056
1.518 ± 0.023
5.245 ± 0.037
6.093 ± 0.049
3.699 ± 0.027
8.276 ± 0.044
2.03 ± 0.022
4.477 ± 0.04
3.839 ± 0.042
11.166 ± 0.069
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.594 ± 0.02
3.005 ± 0.034
5.223 ± 0.04
3.363 ± 0.026
6.984 ± 0.056
5.456 ± 0.03
4.802 ± 0.052
6.859 ± 0.034
1.318 ± 0.018
2.531 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here