Paenibacillus phage phiIBB_Pl23

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fernvirus; Paenibacillus virus P123

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9W0R1|R9W0R1_9CAUD HicB-like domain-containing protein OS=Paenibacillus phage phiIBB_Pl23 OX=1337877 GN=IBBPl23_62 PE=4 SV=1
MM1 pKa = 7.38TKK3 pKa = 10.06QYY5 pKa = 9.8WCEE8 pKa = 3.69EE9 pKa = 4.11CQNFVDD15 pKa = 3.92EE16 pKa = 4.72HH17 pKa = 6.89VVTNGIHH24 pKa = 6.84DD25 pKa = 3.74EE26 pKa = 4.51CGQEE30 pKa = 4.11VNIEE34 pKa = 4.05EE35 pKa = 4.59NEE37 pKa = 3.95EE38 pKa = 4.06DD39 pKa = 4.68DD40 pKa = 4.71LL41 pKa = 6.61

Molecular weight: 4.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9W0P2|R9W0P2_9CAUD HTH cro/C1-type domain-containing protein OS=Paenibacillus phage phiIBB_Pl23 OX=1337877 GN=IBBPl23_32 PE=4 SV=1
MM1 pKa = 6.96KK2 pKa = 9.84TVLFHH7 pKa = 5.99VLSMFNRR14 pKa = 11.84VIINFAFRR22 pKa = 11.84SGGSNEE28 pKa = 4.36RR29 pKa = 11.84GINTYY34 pKa = 8.55NNRR37 pKa = 11.84EE38 pKa = 3.71GHH40 pKa = 5.64YY41 pKa = 10.67LSS43 pKa = 4.67

Molecular weight: 4.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

12525

39

975

184.2

21.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.267 ± 0.507

1.126 ± 0.189

5.9 ± 0.278

8.487 ± 0.44

3.792 ± 0.181

6.084 ± 0.308

1.876 ± 0.169

7.026 ± 0.265

8.535 ± 0.31

8.144 ± 0.258

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.834 ± 0.206

5.062 ± 0.324

3.473 ± 0.207

4.016 ± 0.187

4.743 ± 0.328

6.156 ± 0.354

5.261 ± 0.243

5.948 ± 0.189

1.517 ± 0.176

3.752 ± 0.223
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski