Amino acid dipepetide frequency for Paenibacillus phage phiIBB_Pl23

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.551AlaAla: 4.551 ± 0.956
0.559AlaCys: 0.559 ± 0.179
4.392AlaAsp: 4.392 ± 0.621
6.308AlaGlu: 6.308 ± 0.693
1.916AlaPhe: 1.916 ± 0.407
4.312AlaGly: 4.312 ± 0.664
1.198AlaHis: 1.198 ± 0.305
4.152AlaIle: 4.152 ± 0.825
5.03AlaLys: 5.03 ± 0.705
4.95AlaLeu: 4.95 ± 0.883
1.357AlaMet: 1.357 ± 0.289
3.194AlaAsn: 3.194 ± 0.496
1.437AlaPro: 1.437 ± 0.352
2.156AlaGln: 2.156 ± 0.376
2.395AlaArg: 2.395 ± 0.526
3.513AlaSer: 3.513 ± 0.523
3.593AlaThr: 3.593 ± 0.839
3.753AlaVal: 3.753 ± 0.546
1.118AlaTrp: 1.118 ± 0.354
2.795AlaTyr: 2.795 ± 0.491
0.0AlaXaa: 0.0 ± 0.0
Cys
0.399CysAla: 0.399 ± 0.204
0.24CysCys: 0.24 ± 0.147
0.639CysAsp: 0.639 ± 0.255
0.559CysGlu: 0.559 ± 0.218
0.479CysPhe: 0.479 ± 0.198
1.198CysGly: 1.198 ± 0.332
0.319CysHis: 0.319 ± 0.166
0.399CysIle: 0.399 ± 0.178
1.118CysLys: 1.118 ± 0.395
1.198CysLeu: 1.198 ± 0.313
0.479CysMet: 0.479 ± 0.173
0.399CysAsn: 0.399 ± 0.173
0.559CysPro: 0.559 ± 0.249
0.399CysGln: 0.399 ± 0.189
0.559CysArg: 0.559 ± 0.214
0.479CysSer: 0.479 ± 0.186
0.479CysThr: 0.479 ± 0.174
0.479CysVal: 0.479 ± 0.192
0.319CysTrp: 0.319 ± 0.188
0.559CysTyr: 0.559 ± 0.185
0.0CysXaa: 0.0 ± 0.0
Asp
3.433AspAla: 3.433 ± 0.672
0.399AspCys: 0.399 ± 0.229
4.152AspAsp: 4.152 ± 0.633
4.871AspGlu: 4.871 ± 0.811
3.354AspPhe: 3.354 ± 0.475
4.392AspGly: 4.392 ± 0.729
1.437AspHis: 1.437 ± 0.283
3.513AspIle: 3.513 ± 0.516
3.513AspLys: 3.513 ± 0.579
4.871AspLeu: 4.871 ± 0.478
2.156AspMet: 2.156 ± 0.419
2.795AspAsn: 2.795 ± 0.632
2.475AspPro: 2.475 ± 0.479
2.316AspGln: 2.316 ± 0.45
2.715AspArg: 2.715 ± 0.446
2.954AspSer: 2.954 ± 0.534
2.954AspThr: 2.954 ± 0.434
3.912AspVal: 3.912 ± 0.486
0.559AspTrp: 0.559 ± 0.225
2.236AspTyr: 2.236 ± 0.444
0.0AspXaa: 0.0 ± 0.0
Glu
5.43GluAla: 5.43 ± 0.723
0.399GluCys: 0.399 ± 0.224
5.03GluAsp: 5.03 ± 0.706
7.186GluGlu: 7.186 ± 1.013
3.034GluPhe: 3.034 ± 0.411
4.392GluGly: 4.392 ± 0.518
1.198GluHis: 1.198 ± 0.411
6.707GluIle: 6.707 ± 0.653
7.266GluLys: 7.266 ± 0.788
7.186GluLeu: 7.186 ± 0.763
3.912GluMet: 3.912 ± 0.583
3.912GluAsn: 3.912 ± 0.624
2.316GluPro: 2.316 ± 0.459
4.072GluGln: 4.072 ± 0.708
4.471GluArg: 4.471 ± 0.658
3.593GluSer: 3.593 ± 0.501
4.631GluThr: 4.631 ± 0.611
5.27GluVal: 5.27 ± 0.723
1.038GluTrp: 1.038 ± 0.294
3.833GluTyr: 3.833 ± 0.572
0.0GluXaa: 0.0 ± 0.0
Phe
1.996PheAla: 1.996 ± 0.39
1.357PheCys: 1.357 ± 0.362
2.395PheAsp: 2.395 ± 0.396
3.433PheGlu: 3.433 ± 0.538
1.038PhePhe: 1.038 ± 0.269
2.236PheGly: 2.236 ± 0.429
0.798PheHis: 0.798 ± 0.253
2.954PheIle: 2.954 ± 0.441
3.354PheLys: 3.354 ± 0.617
2.874PheLeu: 2.874 ± 0.408
1.118PheMet: 1.118 ± 0.285
2.156PheAsn: 2.156 ± 0.403
0.798PhePro: 0.798 ± 0.242
1.198PheGln: 1.198 ± 0.3
1.198PheArg: 1.198 ± 0.315
2.874PheSer: 2.874 ± 0.687
1.916PheThr: 1.916 ± 0.323
2.635PheVal: 2.635 ± 0.441
0.319PheTrp: 0.319 ± 0.14
1.278PheTyr: 1.278 ± 0.278
0.0PheXaa: 0.0 ± 0.0
Gly
2.954GlyAla: 2.954 ± 0.523
0.958GlyCys: 0.958 ± 0.359
3.194GlyAsp: 3.194 ± 0.596
5.35GlyGlu: 5.35 ± 0.733
2.555GlyPhe: 2.555 ± 0.595
3.114GlyGly: 3.114 ± 0.531
0.798GlyHis: 0.798 ± 0.249
5.509GlyIle: 5.509 ± 0.983
6.228GlyLys: 6.228 ± 0.66
5.27GlyLeu: 5.27 ± 0.877
1.597GlyMet: 1.597 ± 0.513
3.593GlyAsn: 3.593 ± 0.584
0.559GlyPro: 0.559 ± 0.215
2.715GlyGln: 2.715 ± 0.385
3.114GlyArg: 3.114 ± 0.472
3.833GlySer: 3.833 ± 0.567
2.395GlyThr: 2.395 ± 0.551
3.593GlyVal: 3.593 ± 0.506
1.118GlyTrp: 1.118 ± 0.247
2.395GlyTyr: 2.395 ± 0.378
0.0GlyXaa: 0.0 ± 0.0
His
1.357HisAla: 1.357 ± 0.314
0.639HisCys: 0.639 ± 0.222
0.639HisAsp: 0.639 ± 0.192
1.357HisGlu: 1.357 ± 0.406
0.798HisPhe: 0.798 ± 0.251
0.719HisGly: 0.719 ± 0.228
0.399HisHis: 0.399 ± 0.202
1.836HisIle: 1.836 ± 0.433
0.878HisLys: 0.878 ± 0.317
2.715HisLeu: 2.715 ± 0.501
0.399HisMet: 0.399 ± 0.163
0.958HisAsn: 0.958 ± 0.278
0.958HisPro: 0.958 ± 0.256
0.479HisGln: 0.479 ± 0.207
1.198HisArg: 1.198 ± 0.28
1.118HisSer: 1.118 ± 0.329
0.24HisThr: 0.24 ± 0.136
1.437HisVal: 1.437 ± 0.288
0.24HisTrp: 0.24 ± 0.121
0.399HisTyr: 0.399 ± 0.196
0.0HisXaa: 0.0 ± 0.0
Ile
3.912IleAla: 3.912 ± 0.663
1.198IleCys: 1.198 ± 0.318
4.551IleAsp: 4.551 ± 0.7
7.346IleGlu: 7.346 ± 0.753
3.274IlePhe: 3.274 ± 0.439
3.433IleGly: 3.433 ± 0.619
1.278IleHis: 1.278 ± 0.317
4.152IleIle: 4.152 ± 0.756
6.947IleLys: 6.947 ± 0.894
4.631IleLeu: 4.631 ± 0.472
1.278IleMet: 1.278 ± 0.339
4.072IleAsn: 4.072 ± 0.479
2.316IlePro: 2.316 ± 0.422
2.874IleGln: 2.874 ± 0.461
4.312IleArg: 4.312 ± 0.657
2.954IleSer: 2.954 ± 0.464
3.912IleThr: 3.912 ± 0.428
4.312IleVal: 4.312 ± 0.598
0.719IleTrp: 0.719 ± 0.243
2.795IleTyr: 2.795 ± 0.45
0.0IleXaa: 0.0 ± 0.0
Lys
5.829LysAla: 5.829 ± 0.813
0.319LysCys: 0.319 ± 0.176
4.312LysAsp: 4.312 ± 0.679
8.224LysGlu: 8.224 ± 0.901
2.475LysPhe: 2.475 ± 0.348
4.711LysGly: 4.711 ± 0.678
1.437LysHis: 1.437 ± 0.298
5.989LysIle: 5.989 ± 0.657
7.825LysLys: 7.825 ± 0.994
7.027LysLeu: 7.027 ± 0.711
3.034LysMet: 3.034 ± 0.472
3.912LysAsn: 3.912 ± 0.691
2.874LysPro: 2.874 ± 0.495
3.992LysGln: 3.992 ± 0.607
4.232LysArg: 4.232 ± 0.646
5.19LysSer: 5.19 ± 0.62
5.43LysThr: 5.43 ± 0.605
4.232LysVal: 4.232 ± 0.527
1.198LysTrp: 1.198 ± 0.26
3.114LysTyr: 3.114 ± 0.483
0.0LysXaa: 0.0 ± 0.0
Leu
6.388LeuAla: 6.388 ± 0.743
0.958LeuCys: 0.958 ± 0.286
5.11LeuAsp: 5.11 ± 0.654
6.867LeuGlu: 6.867 ± 0.638
2.954LeuPhe: 2.954 ± 0.515
4.471LeuGly: 4.471 ± 0.598
2.156LeuHis: 2.156 ± 0.447
5.11LeuIle: 5.11 ± 0.51
6.627LeuLys: 6.627 ± 0.68
6.228LeuLeu: 6.228 ± 0.772
1.597LeuMet: 1.597 ± 0.325
3.833LeuAsn: 3.833 ± 0.38
3.593LeuPro: 3.593 ± 0.433
3.673LeuGln: 3.673 ± 0.504
3.673LeuArg: 3.673 ± 0.433
5.589LeuSer: 5.589 ± 0.614
4.711LeuThr: 4.711 ± 0.58
4.072LeuVal: 4.072 ± 0.667
1.118LeuTrp: 1.118 ± 0.311
2.715LeuTyr: 2.715 ± 0.381
0.0LeuXaa: 0.0 ± 0.0
Met
2.316MetAla: 2.316 ± 0.426
0.16MetCys: 0.16 ± 0.089
1.517MetAsp: 1.517 ± 0.34
2.236MetGlu: 2.236 ± 0.456
0.958MetPhe: 0.958 ± 0.25
1.357MetGly: 1.357 ± 0.364
0.0MetHis: 0.0 ± 0.0
1.757MetIle: 1.757 ± 0.386
3.513MetLys: 3.513 ± 0.61
2.076MetLeu: 2.076 ± 0.455
0.479MetMet: 0.479 ± 0.215
2.475MetAsn: 2.475 ± 0.427
0.958MetPro: 0.958 ± 0.277
1.198MetGln: 1.198 ± 0.287
0.958MetArg: 0.958 ± 0.318
2.316MetSer: 2.316 ± 0.424
1.597MetThr: 1.597 ± 0.318
1.038MetVal: 1.038 ± 0.271
0.319MetTrp: 0.319 ± 0.154
1.118MetTyr: 1.118 ± 0.303
0.0MetXaa: 0.0 ± 0.0
Asn
2.316AsnAla: 2.316 ± 0.33
0.559AsnCys: 0.559 ± 0.193
2.635AsnAsp: 2.635 ± 0.557
4.152AsnGlu: 4.152 ± 0.59
1.517AsnPhe: 1.517 ± 0.388
4.711AsnGly: 4.711 ± 0.718
1.357AsnHis: 1.357 ± 0.381
3.433AsnIle: 3.433 ± 0.49
2.475AsnLys: 2.475 ± 0.446
4.312AsnLeu: 4.312 ± 0.547
1.198AsnMet: 1.198 ± 0.436
2.795AsnAsn: 2.795 ± 0.522
2.635AsnPro: 2.635 ± 0.528
2.475AsnGln: 2.475 ± 0.701
3.513AsnArg: 3.513 ± 0.557
3.274AsnSer: 3.274 ± 0.581
2.395AsnThr: 2.395 ± 0.351
2.635AsnVal: 2.635 ± 0.43
0.798AsnTrp: 0.798 ± 0.212
1.437AsnTyr: 1.437 ± 0.286
0.0AsnXaa: 0.0 ± 0.0
Pro
1.757ProAla: 1.757 ± 0.321
0.16ProCys: 0.16 ± 0.112
2.475ProAsp: 2.475 ± 0.415
3.513ProGlu: 3.513 ± 0.689
1.357ProPhe: 1.357 ± 0.374
2.395ProGly: 2.395 ± 0.372
0.958ProHis: 0.958 ± 0.265
2.555ProIle: 2.555 ± 0.376
2.795ProLys: 2.795 ± 0.538
2.395ProLeu: 2.395 ± 0.466
0.719ProMet: 0.719 ± 0.262
1.916ProAsn: 1.916 ± 0.512
1.198ProPro: 1.198 ± 0.27
0.559ProGln: 0.559 ± 0.239
1.038ProArg: 1.038 ± 0.266
2.715ProSer: 2.715 ± 0.583
1.757ProThr: 1.757 ± 0.335
2.555ProVal: 2.555 ± 0.457
0.798ProTrp: 0.798 ± 0.237
1.118ProTyr: 1.118 ± 0.297
0.0ProXaa: 0.0 ± 0.0
Gln
3.274GlnAla: 3.274 ± 0.606
0.319GlnCys: 0.319 ± 0.16
2.076GlnAsp: 2.076 ± 0.306
3.194GlnGlu: 3.194 ± 0.633
1.757GlnPhe: 1.757 ± 0.268
2.795GlnGly: 2.795 ± 0.511
0.798GlnHis: 0.798 ± 0.218
2.795GlnIle: 2.795 ± 0.454
2.874GlnLys: 2.874 ± 0.421
3.593GlnLeu: 3.593 ± 0.569
1.677GlnMet: 1.677 ± 0.41
1.836GlnAsn: 1.836 ± 0.38
1.677GlnPro: 1.677 ± 0.38
1.597GlnGln: 1.597 ± 0.464
2.316GlnArg: 2.316 ± 0.496
2.316GlnSer: 2.316 ± 0.37
2.156GlnThr: 2.156 ± 0.388
1.677GlnVal: 1.677 ± 0.391
0.399GlnTrp: 0.399 ± 0.194
0.958GlnTyr: 0.958 ± 0.301
0.0GlnXaa: 0.0 ± 0.0
Arg
2.635ArgAla: 2.635 ± 0.374
0.559ArgCys: 0.559 ± 0.276
2.555ArgAsp: 2.555 ± 0.459
3.513ArgGlu: 3.513 ± 0.558
1.597ArgPhe: 1.597 ± 0.351
2.715ArgGly: 2.715 ± 0.467
0.798ArgHis: 0.798 ± 0.251
3.194ArgIle: 3.194 ± 0.454
5.11ArgLys: 5.11 ± 0.824
4.152ArgLeu: 4.152 ± 0.479
1.118ArgMet: 1.118 ± 0.299
2.395ArgAsn: 2.395 ± 0.501
2.236ArgPro: 2.236 ± 0.525
2.316ArgGln: 2.316 ± 0.397
2.475ArgArg: 2.475 ± 0.425
2.555ArgSer: 2.555 ± 0.429
2.076ArgThr: 2.076 ± 0.397
2.475ArgVal: 2.475 ± 0.457
1.118ArgTrp: 1.118 ± 0.316
1.836ArgTyr: 1.836 ± 0.364
0.0ArgXaa: 0.0 ± 0.0
Ser
5.03SerAla: 5.03 ± 0.938
0.24SerCys: 0.24 ± 0.133
3.433SerAsp: 3.433 ± 0.486
4.791SerGlu: 4.791 ± 0.607
2.316SerPhe: 2.316 ± 0.459
3.992SerGly: 3.992 ± 0.621
1.038SerHis: 1.038 ± 0.287
5.43SerIle: 5.43 ± 0.726
4.711SerLys: 4.711 ± 0.762
4.711SerLeu: 4.711 ± 0.787
1.836SerMet: 1.836 ± 0.429
2.635SerAsn: 2.635 ± 0.622
2.156SerPro: 2.156 ± 0.396
2.076SerGln: 2.076 ± 0.477
2.555SerArg: 2.555 ± 0.541
4.551SerSer: 4.551 ± 0.891
4.072SerThr: 4.072 ± 0.557
3.673SerVal: 3.673 ± 0.694
0.559SerTrp: 0.559 ± 0.205
1.757SerTyr: 1.757 ± 0.313
0.0SerXaa: 0.0 ± 0.0
Thr
3.274ThrAla: 3.274 ± 0.801
0.559ThrCys: 0.559 ± 0.207
2.874ThrAsp: 2.874 ± 0.393
2.795ThrGlu: 2.795 ± 0.48
1.757ThrPhe: 1.757 ± 0.343
4.072ThrGly: 4.072 ± 0.585
1.038ThrHis: 1.038 ± 0.301
3.673ThrIle: 3.673 ± 0.534
4.95ThrLys: 4.95 ± 0.527
3.992ThrLeu: 3.992 ± 0.669
1.517ThrMet: 1.517 ± 0.355
2.076ThrAsn: 2.076 ± 0.366
2.475ThrPro: 2.475 ± 0.429
2.156ThrGln: 2.156 ± 0.395
1.757ThrArg: 1.757 ± 0.332
4.312ThrSer: 4.312 ± 0.638
2.236ThrThr: 2.236 ± 0.45
4.152ThrVal: 4.152 ± 0.624
0.559ThrTrp: 0.559 ± 0.244
2.395ThrTyr: 2.395 ± 0.435
0.0ThrXaa: 0.0 ± 0.0
Val
3.194ValAla: 3.194 ± 0.539
0.878ValCys: 0.878 ± 0.287
3.114ValAsp: 3.114 ± 0.472
5.03ValGlu: 5.03 ± 0.599
2.874ValPhe: 2.874 ± 0.442
2.954ValGly: 2.954 ± 0.503
0.559ValHis: 0.559 ± 0.25
3.912ValIle: 3.912 ± 0.426
5.35ValLys: 5.35 ± 0.578
4.392ValLeu: 4.392 ± 0.493
1.757ValMet: 1.757 ± 0.351
2.156ValAsn: 2.156 ± 0.527
2.395ValPro: 2.395 ± 0.438
1.757ValGln: 1.757 ± 0.37
2.076ValArg: 2.076 ± 0.438
4.711ValSer: 4.711 ± 0.561
4.152ValThr: 4.152 ± 0.602
3.833ValVal: 3.833 ± 0.47
1.996ValTrp: 1.996 ± 0.821
2.395ValTyr: 2.395 ± 0.563
0.0ValXaa: 0.0 ± 0.0
Trp
0.958TrpAla: 0.958 ± 0.233
0.319TrpCys: 0.319 ± 0.163
1.038TrpAsp: 1.038 ± 0.32
1.038TrpGlu: 1.038 ± 0.224
0.639TrpPhe: 0.639 ± 0.2
0.719TrpGly: 0.719 ± 0.244
0.559TrpHis: 0.559 ± 0.18
0.878TrpIle: 0.878 ± 0.289
1.357TrpLys: 1.357 ± 0.306
1.517TrpLeu: 1.517 ± 0.349
0.08TrpMet: 0.08 ± 0.074
1.996TrpAsn: 1.996 ± 0.948
0.08TrpPro: 0.08 ± 0.07
0.559TrpGln: 0.559 ± 0.188
0.639TrpArg: 0.639 ± 0.236
0.719TrpSer: 0.719 ± 0.245
0.479TrpThr: 0.479 ± 0.212
0.719TrpVal: 0.719 ± 0.232
0.24TrpTrp: 0.24 ± 0.126
0.639TrpTyr: 0.639 ± 0.226
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.677TyrAla: 1.677 ± 0.409
0.479TyrCys: 0.479 ± 0.201
2.874TyrAsp: 2.874 ± 0.473
3.114TyrGlu: 3.114 ± 0.396
1.278TyrPhe: 1.278 ± 0.314
2.156TyrGly: 2.156 ± 0.478
0.639TyrHis: 0.639 ± 0.25
2.316TyrIle: 2.316 ± 0.389
3.433TyrLys: 3.433 ± 0.464
3.354TyrLeu: 3.354 ± 0.486
0.958TyrMet: 0.958 ± 0.3
1.517TyrAsn: 1.517 ± 0.371
1.038TyrPro: 1.038 ± 0.291
1.597TyrGln: 1.597 ± 0.388
2.236TyrArg: 2.236 ± 0.404
1.996TyrSer: 1.996 ± 0.374
1.437TyrThr: 1.437 ± 0.322
3.034TyrVal: 3.034 ± 0.443
0.639TyrTrp: 0.639 ± 0.281
1.757TyrTyr: 1.757 ± 0.383
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 68 proteins (12525 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski