Neonectria ditissima

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Hypocreales; Nectriaceae; Neonectria

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12683 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P7AKL7|A0A0P7AKL7_9HYPO Flavin mononucleotide kinase 1 OS=Neonectria ditissima OX=78410 GN=AK830_g8357 PE=3 SV=1
MM1 pKa = 7.55KK2 pKa = 9.77STVAALGMFAAAANAYY18 pKa = 9.46SYY20 pKa = 9.88PRR22 pKa = 11.84HH23 pKa = 3.97VHH25 pKa = 4.31YY26 pKa = 10.83RR27 pKa = 11.84RR28 pKa = 11.84DD29 pKa = 3.4NGTEE33 pKa = 3.88SGLTTLTVLTTQVQTITSCAPTVTNCPARR62 pKa = 11.84DD63 pKa = 3.47QTAIDD68 pKa = 3.95QLPEE72 pKa = 3.59TDD74 pKa = 3.1KK75 pKa = 11.15TVVVVTNTVVLTEE88 pKa = 4.37TVCPVADD95 pKa = 3.72ASSISSEE102 pKa = 4.3VIEE105 pKa = 4.09QAQTGGITGYY115 pKa = 8.71TLTAPLTTSLGPISTGEE132 pKa = 4.09SSPEE136 pKa = 3.63ATTDD140 pKa = 3.4AEE142 pKa = 4.27LTTIVSDD149 pKa = 3.82YY150 pKa = 10.54VTDD153 pKa = 3.53KK154 pKa = 11.03TLTLTIGTGTDD165 pKa = 3.04ASVVTTTVQSTVQTTVTVPCSEE187 pKa = 4.05VSEE190 pKa = 4.42GAEE193 pKa = 3.96ATDD196 pKa = 4.8DD197 pKa = 4.03GSSNGNGSADD207 pKa = 3.78EE208 pKa = 4.29PTTTTTGTKK217 pKa = 9.78TSTLTLTISKK227 pKa = 10.45ADD229 pKa = 3.47STEE232 pKa = 3.91TKK234 pKa = 9.62TAGDD238 pKa = 3.4NSGNGTPEE246 pKa = 4.1GSSGAEE252 pKa = 3.87GSSEE256 pKa = 4.11GACADD261 pKa = 3.03ATTTVTVTAPASTVYY276 pKa = 9.17VTVGSDD282 pKa = 3.02HH283 pKa = 6.79TGTVGAEE290 pKa = 4.09GTKK293 pKa = 9.58TGSSGSTEE301 pKa = 4.11TGSDD305 pKa = 3.66SNDD308 pKa = 3.74DD309 pKa = 4.06EE310 pKa = 7.02DD311 pKa = 6.67DD312 pKa = 4.11EE313 pKa = 7.25DD314 pKa = 6.42DD315 pKa = 5.57EE316 pKa = 7.27DD317 pKa = 6.42DD318 pKa = 4.72EE319 pKa = 6.25DD320 pKa = 4.22YY321 pKa = 11.76CEE323 pKa = 5.64DD324 pKa = 4.85DD325 pKa = 5.57DD326 pKa = 4.85VTTTLEE332 pKa = 3.94ATVTVVPYY340 pKa = 9.49PVNSTFTGGYY350 pKa = 8.64AAPTGFARR358 pKa = 11.84LVRR361 pKa = 4.2

Molecular weight: 36.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N8H4N9|A0A0N8H4N9_9HYPO Uncharacterized protein OS=Neonectria ditissima OX=78410 GN=AK830_g12672 PE=4 SV=1
MM1 pKa = 8.05PITRR5 pKa = 11.84THH7 pKa = 6.27PHH9 pKa = 4.98TTTRR13 pKa = 11.84APRR16 pKa = 11.84ATRR19 pKa = 11.84APRR22 pKa = 11.84KK23 pKa = 10.25SMFGRR28 pKa = 11.84RR29 pKa = 11.84AAPRR33 pKa = 11.84NHH35 pKa = 5.3QQAVTTTTTTTTTRR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84GFFGTKK57 pKa = 9.7KK58 pKa = 9.99PQTTRR63 pKa = 11.84DD64 pKa = 3.39TVVQQHH70 pKa = 6.59RR71 pKa = 11.84KK72 pKa = 7.83PTMKK76 pKa = 10.59DD77 pKa = 3.04KK78 pKa = 11.51VSGALLKK85 pKa = 11.04LKK87 pKa = 10.63GSLTHH92 pKa = 6.82RR93 pKa = 11.84PGVKK97 pKa = 9.85AAGTRR102 pKa = 11.84RR103 pKa = 11.84MHH105 pKa = 5.67GTDD108 pKa = 2.89GRR110 pKa = 11.84GTRR113 pKa = 11.84RR114 pKa = 11.84HH115 pKa = 6.44AII117 pKa = 3.54

Molecular weight: 13.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12683

0

12683

6561620

18

11921

517.4

57.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.866 ± 0.022

1.287 ± 0.021

5.945 ± 0.017

6.052 ± 0.023

3.759 ± 0.015

6.927 ± 0.019

2.396 ± 0.01

4.75 ± 0.015

4.668 ± 0.019

8.931 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.192 ± 0.009

3.519 ± 0.012

6.1 ± 0.027

3.964 ± 0.018

6.028 ± 0.019

8.118 ± 0.021

5.998 ± 0.02

6.293 ± 0.015

1.538 ± 0.008

2.668 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski