Flavonifractor sp. An52
Average proteome isoelectric point is 6.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2620 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y3XK30|A0A1Y3XK30_9FIRM Ornithine carbamoyltransferase OS=Flavonifractor sp. An52 OX=1965642 GN=B5G06_02190 PE=3 SV=1
MM1 pKa = 7.73 KK2 pKa = 10.38 NFKK5 pKa = 10.19 KK6 pKa = 10.29 LALMGLTAVMSLSLLAACGGGNSGSAEE33 pKa = 4.14 TPSGNTEE40 pKa = 4.03 TPSVEE45 pKa = 4.14 NTEE48 pKa = 4.15 TPAASGEE55 pKa = 4.28 FTTVEE60 pKa = 3.75 AGKK63 pKa = 10.73 LIMSTNAQFPPYY75 pKa = 10.44 EE76 pKa = 4.03 MVGDD80 pKa = 3.94 GEE82 pKa = 5.04 GYY84 pKa = 10.52 EE85 pKa = 4.27 GTGYY89 pKa = 10.8 EE90 pKa = 4.57 GIDD93 pKa = 3.36 VEE95 pKa = 4.18 IAYY98 pKa = 10.12 AIAQKK103 pKa = 10.73 LGLEE107 pKa = 4.01 LVVDD111 pKa = 3.79 NMGFDD116 pKa = 3.73 AALQAVQTGKK126 pKa = 10.73 SDD128 pKa = 2.72 IVMAGVTVTDD138 pKa = 4.03 DD139 pKa = 3.4 RR140 pKa = 11.84 LEE142 pKa = 4.18 VMDD145 pKa = 6.64 FSDD148 pKa = 4.15 TYY150 pKa = 11.65 ANGIQVVIVPEE161 pKa = 4.12 NSDD164 pKa = 2.89 IATIDD169 pKa = 3.69 DD170 pKa = 4.75 LEE172 pKa = 4.98 GKK174 pKa = 10.51 LIGCQMGTTGYY185 pKa = 9.32 IYY187 pKa = 10.83 CSDD190 pKa = 3.5 TPEE193 pKa = 4.06 NGGYY197 pKa = 10.75 GEE199 pKa = 4.95 DD200 pKa = 3.92 AVIPYY205 pKa = 10.28 DD206 pKa = 4.43 DD207 pKa = 4.25 GAAAIQALMNGQVDD221 pKa = 4.08 CVVIDD226 pKa = 3.6 NAPAQEE232 pKa = 4.1 YY233 pKa = 10.44 VKK235 pKa = 10.82 ANPGLKK241 pKa = 9.79 ILDD244 pKa = 3.56 TEE246 pKa = 4.57 FANEE250 pKa = 3.51 NYY252 pKa = 10.6 AIGFAKK258 pKa = 10.58 GNTALKK264 pKa = 10.58 DD265 pKa = 3.78 AVNTALTEE273 pKa = 4.58 LIEE276 pKa = 5.15 DD277 pKa = 3.94 GTVQSIIDD285 pKa = 3.73 KK286 pKa = 9.72 YY287 pKa = 11.0 IPAEE291 pKa = 4.0 GG292 pKa = 3.39
Molecular weight: 30.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.724
IPC2_protein 3.732
IPC_protein 3.732
Toseland 3.516
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.617
Rodwell 3.554
Grimsley 3.427
Solomon 3.694
Lehninger 3.656
Nozaki 3.821
DTASelect 4.012
Thurlkill 3.567
EMBOSS 3.63
Sillero 3.846
Patrickios 0.846
IPC_peptide 3.694
IPC2_peptide 3.821
IPC2.peptide.svr19 3.751
Protein with the highest isoelectric point:
>tr|A0A1Y3XKT9|A0A1Y3XKT9_9FIRM Shikimate dehydrogenase (NADP(+)) OS=Flavonifractor sp. An52 OX=1965642 GN=aroE PE=3 SV=1
MM1 pKa = 7.06 VRR3 pKa = 11.84 TYY5 pKa = 10.22 QPKK8 pKa = 9.24 KK9 pKa = 7.68 RR10 pKa = 11.84 QRR12 pKa = 11.84 SKK14 pKa = 9.69 EE15 pKa = 3.5 HH16 pKa = 6.13 GFRR19 pKa = 11.84 KK20 pKa = 9.96 RR21 pKa = 11.84 MRR23 pKa = 11.84 TANGRR28 pKa = 11.84 KK29 pKa = 8.55 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.04 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LTHH44 pKa = 6.28
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.354
IPC2_protein 10.906
IPC_protein 12.325
Toseland 12.486
ProMoST 12.983
Dawson 12.486
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.193
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 11.93
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.081
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2620
0
2620
823819
32
2954
314.4
34.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.578 ± 0.054
1.638 ± 0.021
5.669 ± 0.044
6.696 ± 0.05
3.642 ± 0.032
8.178 ± 0.058
1.861 ± 0.023
5.416 ± 0.037
4.698 ± 0.048
10.152 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.693 ± 0.025
3.234 ± 0.035
4.377 ± 0.034
3.653 ± 0.032
5.488 ± 0.055
5.314 ± 0.04
5.744 ± 0.063
7.402 ± 0.047
1.151 ± 0.02
3.416 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here