Jasmine virus C
Average proteome isoelectric point is 7.05
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A173G8H3|A0A173G8H3_9VIRU 7 kDa protein OS=Jasmine virus C OX=1853762 GN=TGB3 PE=3 SV=1
MM1 pKa = 7.58 EE2 pKa = 5.82 LLLNKK7 pKa = 10.23 LEE9 pKa = 4.5 SFGFTRR15 pKa = 11.84 VSSALRR21 pKa = 11.84 LPLIVHH27 pKa = 6.57 CVPGAGKK34 pKa = 10.33 SSLIRR39 pKa = 11.84 AILEE43 pKa = 4.08 DD44 pKa = 4.15 SVEE47 pKa = 3.78 LRR49 pKa = 11.84 AYY51 pKa = 9.45 TFGQADD57 pKa = 3.82 QPNLIGNYY65 pKa = 8.8 IRR67 pKa = 11.84 PFTSDD72 pKa = 3.44 SILDD76 pKa = 3.32 QRR78 pKa = 11.84 TIIDD82 pKa = 4.61 EE83 pKa = 4.46 YY84 pKa = 10.49 TLSPQPIHH92 pKa = 6.8 GVLALFGDD100 pKa = 4.3 PRR102 pKa = 11.84 QPGVDD107 pKa = 3.11 QGLVANFLGNFSRR120 pKa = 11.84 RR121 pKa = 11.84 FGANTAIFLRR131 pKa = 11.84 KK132 pKa = 9.81 LGFNVHH138 pKa = 6.84 AEE140 pKa = 4.23 GEE142 pKa = 4.39 DD143 pKa = 3.61 TVRR146 pKa = 11.84 VLDD149 pKa = 3.62 IFKK152 pKa = 10.58 AEE154 pKa = 3.89 PSGVIVCFEE163 pKa = 4.36 PEE165 pKa = 4.22 VQKK168 pKa = 10.76 LLCAHH173 pKa = 5.97 NLEE176 pKa = 4.53 YY177 pKa = 10.25 LTAQEE182 pKa = 4.3 IQGSTFQEE190 pKa = 4.05 VSFIVSGPFQIEE202 pKa = 3.69 RR203 pKa = 11.84 ARR205 pKa = 11.84 EE206 pKa = 3.78 HH207 pKa = 6.23 FLCLTRR213 pKa = 11.84 HH214 pKa = 5.99 RR215 pKa = 11.84 ATLNILCPDD224 pKa = 3.38 ATYY227 pKa = 7.61 TTSS230 pKa = 2.8
Molecular weight: 25.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.482
IPC2_protein 5.486
IPC_protein 5.474
Toseland 5.741
ProMoST 5.741
Dawson 5.639
Bjellqvist 5.677
Wikipedia 5.601
Rodwell 5.614
Grimsley 5.842
Solomon 5.639
Lehninger 5.626
Nozaki 5.868
DTASelect 6.033
Thurlkill 5.982
EMBOSS 5.944
Sillero 5.957
Patrickios 4.062
IPC_peptide 5.652
IPC2_peptide 5.982
IPC2.peptide.svr19 5.909
Protein with the highest isoelectric point:
>tr|A0A173G8K3|A0A173G8K3_9VIRU Movement protein TGB2 OS=Jasmine virus C OX=1853762 GN=TGB2 PE=3 SV=1
MM1 pKa = 7.82 RR2 pKa = 11.84 YY3 pKa = 8.45 EE4 pKa = 4.35 LEE6 pKa = 3.98 VALVINRR13 pKa = 11.84 VFQARR18 pKa = 11.84 GVHH21 pKa = 5.87 NLALALYY28 pKa = 8.88 ISKK31 pKa = 10.38 KK32 pKa = 10.64 AVGPCVNNGRR42 pKa = 11.84 STYY45 pKa = 10.3 ARR47 pKa = 11.84 RR48 pKa = 11.84 RR49 pKa = 11.84 RR50 pKa = 11.84 AKK52 pKa = 10.37 SISRR56 pKa = 11.84 CYY58 pKa = 9.25 RR59 pKa = 11.84 CYY61 pKa = 10.04 RR62 pKa = 11.84 VYY64 pKa = 10.69 PPLCGNTRR72 pKa = 11.84 CNGRR76 pKa = 11.84 TCFPGINYY84 pKa = 9.83 RR85 pKa = 11.84 VDD87 pKa = 3.03 VEE89 pKa = 4.4 KK90 pKa = 11.06 YY91 pKa = 9.79 IKK93 pKa = 10.48 FGVAAAIPNFKK104 pKa = 10.51 LL105 pKa = 3.44
Molecular weight: 12.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.322
IPC2_protein 9.838
IPC_protein 10.526
Toseland 10.248
ProMoST 10.087
Dawson 10.482
Bjellqvist 10.262
Wikipedia 10.716
Rodwell 10.672
Grimsley 10.584
Solomon 10.54
Lehninger 10.496
Nozaki 10.321
DTASelect 10.233
Thurlkill 10.335
EMBOSS 10.672
Sillero 10.423
Patrickios 10.218
IPC_peptide 10.526
IPC2_peptide 9.56
IPC2.peptide.svr19 8.274
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2766
61
1966
461.0
51.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.195 ± 0.976
2.675 ± 0.425
4.736 ± 0.629
7.014 ± 1.103
5.748 ± 0.617
6.616 ± 0.419
2.422 ± 0.171
5.315 ± 0.818
5.676 ± 1.046
9.4 ± 0.794
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.916 ± 0.353
3.724 ± 0.544
4.338 ± 0.81
3.471 ± 0.539
6.471 ± 0.674
6.508 ± 0.332
4.808 ± 0.386
7.701 ± 0.609
0.904 ± 0.293
3.29 ± 0.415
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here