Pseudorhodobacter sp. E13
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3709 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3S1AMU9|A0A3S1AMU9_9RHOB Methyltransferase OS=Pseudorhodobacter sp. E13 OX=2487931 GN=EGN72_11845 PE=3 SV=1
MM1 pKa = 6.52 VTNTNTNPGAQTNQTITASSGTDD24 pKa = 3.05 TLTGGLGNDD33 pKa = 4.76 TINGLAGNDD42 pKa = 3.7 FLRR45 pKa = 11.84 GDD47 pKa = 4.13 GAVQGAWHH55 pKa = 6.7 YY56 pKa = 7.58 EE57 pKa = 3.79 TFNYY61 pKa = 10.11 NFSSANGQAFTPAAFTTATRR81 pKa = 11.84 TGSGYY86 pKa = 8.81 VTDD89 pKa = 4.47 FDD91 pKa = 4.53 EE92 pKa = 5.88 GGITNTMRR100 pKa = 11.84 GVAASTNPEE109 pKa = 4.15 DD110 pKa = 3.83 FGVVYY115 pKa = 10.29 TSTLNVTTGGTYY127 pKa = 10.75 RR128 pKa = 11.84 LTTSSDD134 pKa = 3.42 DD135 pKa = 3.49 GSTVQIFNSAGVPLNFSNQTGGTLNYY161 pKa = 10.06 LNNDD165 pKa = 3.62 FHH167 pKa = 8.64 QSTTTRR173 pKa = 11.84 WGDD176 pKa = 3.3 VVLAPGQTYY185 pKa = 8.59 TIQIRR190 pKa = 11.84 YY191 pKa = 7.76 WEE193 pKa = 4.21 NLGADD198 pKa = 3.78 TLAATINGPDD208 pKa = 3.38 TGGATQNLLTSPMLGLPPGPSYY230 pKa = 11.37 SVTGTAMGVEE240 pKa = 4.6 GNDD243 pKa = 3.59 VLDD246 pKa = 4.55 GGAGNDD252 pKa = 3.84 TIYY255 pKa = 11.3 GDD257 pKa = 4.05 GGNDD261 pKa = 3.49 SLLGGADD268 pKa = 3.39 NDD270 pKa = 4.11 LLYY273 pKa = 11.13 GGTGNDD279 pKa = 3.37 TLFGGTGNDD288 pKa = 3.31 TLYY291 pKa = 11.33 GDD293 pKa = 5.17 DD294 pKa = 5.67 SSDD297 pKa = 4.13 SLNGDD302 pKa = 3.85 DD303 pKa = 5.81 GTDD306 pKa = 3.5 LLYY309 pKa = 11.19 GGAGNDD315 pKa = 3.89 TLNGGAGADD324 pKa = 3.65 TLYY327 pKa = 11.32 GDD329 pKa = 5.41 DD330 pKa = 4.93 GNDD333 pKa = 3.55 SLRR336 pKa = 11.84 GDD338 pKa = 4.03 ADD340 pKa = 3.6 NDD342 pKa = 3.99 TLYY345 pKa = 11.28 GGAGLDD351 pKa = 3.89 TLNGDD356 pKa = 4.52 DD357 pKa = 5.82 GNDD360 pKa = 3.52 ALYY363 pKa = 10.92 GGADD367 pKa = 3.5 ADD369 pKa = 3.86 QLFGGLGSDD378 pKa = 3.84 SLFGGTGADD387 pKa = 3.18 TLYY390 pKa = 11.08 GGDD393 pKa = 4.06 GADD396 pKa = 4.22 SLSGDD401 pKa = 4.6 DD402 pKa = 5.35 GNDD405 pKa = 3.32 SLFGGLGDD413 pKa = 3.96 DD414 pKa = 4.07 TLLGGLGNDD423 pKa = 3.76 TLYY426 pKa = 11.43 GDD428 pKa = 5.45 DD429 pKa = 5.76 GNDD432 pKa = 3.73 SLNGDD437 pKa = 4.3 AGNDD441 pKa = 3.69 SLFGGAGNDD450 pKa = 3.84 TLNGGAGNNTLTGGAGNDD468 pKa = 3.05 RR469 pKa = 11.84 FIYY472 pKa = 9.98 TIGSNLTISDD482 pKa = 4.47 LNLGNSGGILDD493 pKa = 5.19 GDD495 pKa = 3.68 QTNNDD500 pKa = 4.04 FLDD503 pKa = 4.06 LSAFYY508 pKa = 10.85 TNLNEE513 pKa = 4.89 LRR515 pKa = 11.84 DD516 pKa = 4.19 DD517 pKa = 4.29 FSDD520 pKa = 5.28 DD521 pKa = 4.17 GILNQSVGDD530 pKa = 3.98 YY531 pKa = 11.08 SDD533 pKa = 3.36 NTALGGSIILTGITRR548 pKa = 11.84 TDD550 pKa = 3.25 LTTDD554 pKa = 4.23 NINVACFTAGTQIEE568 pKa = 4.67 TADD571 pKa = 3.89 GPALIEE577 pKa = 4.25 SLTAGTLIRR586 pKa = 11.84 TLDD589 pKa = 3.56 HH590 pKa = 6.75 GLQPLRR596 pKa = 11.84 AVLTRR601 pKa = 11.84 SVPGDD606 pKa = 3.28 GRR608 pKa = 11.84 FAPIRR613 pKa = 11.84 FHH615 pKa = 7.66 AGALGNHH622 pKa = 6.43 RR623 pKa = 11.84 AFSTSPAHH631 pKa = 7.21 RR632 pKa = 11.84 MLIADD637 pKa = 4.09 WRR639 pKa = 11.84 AEE641 pKa = 3.86 LLFGEE646 pKa = 4.54 SEE648 pKa = 4.38 VLISARR654 pKa = 11.84 ALVNDD659 pKa = 4.08 CTITRR664 pKa = 11.84 APCDD668 pKa = 2.99 RR669 pKa = 11.84 VTYY672 pKa = 10.01 YY673 pKa = 10.94 HH674 pKa = 7.17 LLLDD678 pKa = 3.31 RR679 pKa = 11.84 HH680 pKa = 6.18 EE681 pKa = 5.06 IIFAEE686 pKa = 5.6 GIATEE691 pKa = 4.88 SYY693 pKa = 9.33 QHH695 pKa = 6.59 GAFADD700 pKa = 3.98 DD701 pKa = 4.35 PGVEE705 pKa = 4.25 AEE707 pKa = 4.98 LDD709 pKa = 3.54 ALFPGLLPAPSDD721 pKa = 3.52 SARR724 pKa = 11.84 PSLRR728 pKa = 11.84 GYY730 pKa = 9.16 EE731 pKa = 3.98 AAALLSLPQRR741 pKa = 11.84 RR742 pKa = 11.84 AAA744 pKa = 4.17
Molecular weight: 76.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.679
IPC2_protein 3.605
IPC_protein 3.681
Toseland 3.427
ProMoST 3.859
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.668
Rodwell 3.49
Grimsley 3.325
Solomon 3.694
Lehninger 3.643
Nozaki 3.795
DTASelect 4.126
Thurlkill 3.49
EMBOSS 3.668
Sillero 3.808
Patrickios 1.481
IPC_peptide 3.681
IPC2_peptide 3.77
IPC2.peptide.svr19 3.729
Protein with the highest isoelectric point:
>tr|A0A3S0YYZ7|A0A3S0YYZ7_9RHOB DUF1501 domain-containing protein OS=Pseudorhodobacter sp. E13 OX=2487931 GN=EGN72_07320 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.58 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATKK25 pKa = 10.33 GGRR28 pKa = 11.84 IVLNNRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.11 GRR39 pKa = 11.84 KK40 pKa = 8.82 KK41 pKa = 10.63 LSAA44 pKa = 3.95
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3709
0
3709
1156015
25
3543
311.7
33.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.292 ± 0.061
0.887 ± 0.013
5.736 ± 0.046
5.418 ± 0.037
3.716 ± 0.028
8.787 ± 0.053
2.005 ± 0.02
5.008 ± 0.026
3.342 ± 0.038
10.282 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.782 ± 0.024
2.582 ± 0.026
5.209 ± 0.037
3.319 ± 0.019
6.506 ± 0.044
4.99 ± 0.031
5.492 ± 0.051
7.067 ± 0.034
1.399 ± 0.017
2.182 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here