Amino acid dipepetide frequency for Pseudorhodobacter sp. E13

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.263AlaAla: 19.263 ± 0.199
1.152AlaCys: 1.152 ± 0.034
7.24AlaAsp: 7.24 ± 0.086
8.653AlaGlu: 8.653 ± 0.12
4.541AlaPhe: 4.541 ± 0.066
11.143AlaGly: 11.143 ± 0.143
2.453AlaHis: 2.453 ± 0.057
5.96AlaIle: 5.96 ± 0.072
4.76AlaLys: 4.76 ± 0.079
14.999AlaLeu: 14.999 ± 0.15
3.989AlaMet: 3.989 ± 0.064
2.905AlaAsn: 2.905 ± 0.051
6.459AlaPro: 6.459 ± 0.097
5.375AlaGln: 5.375 ± 0.076
9.016AlaArg: 9.016 ± 0.11
5.676AlaSer: 5.676 ± 0.075
6.427AlaThr: 6.427 ± 0.105
8.769AlaVal: 8.769 ± 0.093
1.449AlaTrp: 1.449 ± 0.04
2.694AlaTyr: 2.694 ± 0.046
0.0AlaXaa: 0.0 ± 0.0
Cys
1.133CysAla: 1.133 ± 0.029
0.118CysCys: 0.118 ± 0.01
0.565CysAsp: 0.565 ± 0.021
0.403CysGlu: 0.403 ± 0.019
0.356CysPhe: 0.356 ± 0.019
0.925CysGly: 0.925 ± 0.031
0.274CysHis: 0.274 ± 0.018
0.413CysIle: 0.413 ± 0.02
0.24CysLys: 0.24 ± 0.015
0.909CysLeu: 0.909 ± 0.033
0.182CysMet: 0.182 ± 0.012
0.22CysAsn: 0.22 ± 0.012
0.538CysPro: 0.538 ± 0.022
0.257CysGln: 0.257 ± 0.016
0.529CysArg: 0.529 ± 0.023
0.413CysSer: 0.413 ± 0.019
0.451CysThr: 0.451 ± 0.024
0.605CysVal: 0.605 ± 0.026
0.123CysTrp: 0.123 ± 0.011
0.214CysTyr: 0.214 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
7.262AspAla: 7.262 ± 0.087
0.515AspCys: 0.515 ± 0.021
3.045AspAsp: 3.045 ± 0.086
2.894AspGlu: 2.894 ± 0.06
2.235AspPhe: 2.235 ± 0.047
5.235AspGly: 5.235 ± 0.101
1.332AspHis: 1.332 ± 0.035
2.939AspIle: 2.939 ± 0.054
1.704AspLys: 1.704 ± 0.039
6.458AspLeu: 6.458 ± 0.092
1.611AspMet: 1.611 ± 0.036
1.184AspAsn: 1.184 ± 0.043
3.432AspPro: 3.432 ± 0.06
1.909AspGln: 1.909 ± 0.05
3.976AspArg: 3.976 ± 0.061
2.242AspSer: 2.242 ± 0.064
2.798AspThr: 2.798 ± 0.083
3.861AspVal: 3.861 ± 0.066
1.204AspTrp: 1.204 ± 0.035
1.527AspTyr: 1.527 ± 0.038
0.0AspXaa: 0.0 ± 0.0
Glu
8.482GluAla: 8.482 ± 0.104
0.347GluCys: 0.347 ± 0.017
2.814GluAsp: 2.814 ± 0.055
2.887GluGlu: 2.887 ± 0.071
1.715GluPhe: 1.715 ± 0.04
4.541GluGly: 4.541 ± 0.073
0.953GluHis: 0.953 ± 0.028
3.152GluIle: 3.152 ± 0.052
2.075GluLys: 2.075 ± 0.052
4.61GluLeu: 4.61 ± 0.067
1.728GluMet: 1.728 ± 0.04
1.591GluAsn: 1.591 ± 0.038
2.331GluPro: 2.331 ± 0.051
1.774GluGln: 1.774 ± 0.045
3.598GluArg: 3.598 ± 0.068
2.071GluSer: 2.071 ± 0.039
3.543GluThr: 3.543 ± 0.051
4.224GluVal: 4.224 ± 0.071
0.719GluTrp: 0.719 ± 0.024
1.023GluTyr: 1.023 ± 0.031
0.0GluXaa: 0.0 ± 0.0
Phe
4.829PheAla: 4.829 ± 0.075
0.442PheCys: 0.442 ± 0.019
2.826PheAsp: 2.826 ± 0.049
2.041PheGlu: 2.041 ± 0.049
1.401PhePhe: 1.401 ± 0.041
3.879PheGly: 3.879 ± 0.06
0.756PheHis: 0.756 ± 0.026
1.596PheIle: 1.596 ± 0.039
1.022PheLys: 1.022 ± 0.033
3.416PheLeu: 3.416 ± 0.066
0.841PheMet: 0.841 ± 0.033
1.014PheAsn: 1.014 ± 0.029
1.588PhePro: 1.588 ± 0.038
1.051PheGln: 1.051 ± 0.03
2.088PheArg: 2.088 ± 0.05
2.061PheSer: 2.061 ± 0.045
2.218PheThr: 2.218 ± 0.056
2.589PheVal: 2.589 ± 0.048
0.598PheTrp: 0.598 ± 0.028
0.901PheTyr: 0.901 ± 0.027
0.0PheXaa: 0.0 ± 0.0
Gly
10.555GlyAla: 10.555 ± 0.147
0.845GlyCys: 0.845 ± 0.029
4.559GlyAsp: 4.559 ± 0.122
4.171GlyGlu: 4.171 ± 0.071
3.939GlyPhe: 3.939 ± 0.059
7.641GlyGly: 7.641 ± 0.161
1.92GlyHis: 1.92 ± 0.049
4.287GlyIle: 4.287 ± 0.071
3.444GlyLys: 3.444 ± 0.068
9.549GlyLeu: 9.549 ± 0.117
2.653GlyMet: 2.653 ± 0.056
2.375GlyAsn: 2.375 ± 0.094
3.836GlyPro: 3.836 ± 0.059
3.436GlyGln: 3.436 ± 0.056
5.614GlyArg: 5.614 ± 0.079
4.164GlySer: 4.164 ± 0.077
4.721GlyThr: 4.721 ± 0.155
6.439GlyVal: 6.439 ± 0.082
1.482GlyTrp: 1.482 ± 0.033
2.241GlyTyr: 2.241 ± 0.041
0.0GlyXaa: 0.0 ± 0.0
His
2.302HisAla: 2.302 ± 0.053
0.236HisCys: 0.236 ± 0.014
1.157HisAsp: 1.157 ± 0.03
0.955HisGlu: 0.955 ± 0.031
0.841HisPhe: 0.841 ± 0.03
1.819HisGly: 1.819 ± 0.041
0.545HisHis: 0.545 ± 0.026
1.009HisIle: 1.009 ± 0.033
0.524HisLys: 0.524 ± 0.021
2.312HisLeu: 2.312 ± 0.055
0.525HisMet: 0.525 ± 0.023
0.459HisAsn: 0.459 ± 0.024
1.392HisPro: 1.392 ± 0.04
0.588HisGln: 0.588 ± 0.024
1.318HisArg: 1.318 ± 0.037
1.048HisSer: 1.048 ± 0.029
0.71HisThr: 0.71 ± 0.025
1.376HisVal: 1.376 ± 0.03
0.38HisTrp: 0.38 ± 0.019
0.551HisTyr: 0.551 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
7.263IleAla: 7.263 ± 0.086
0.596IleCys: 0.596 ± 0.025
3.174IleAsp: 3.174 ± 0.051
3.047IleGlu: 3.047 ± 0.052
1.702IlePhe: 1.702 ± 0.042
4.883IleGly: 4.883 ± 0.073
0.889IleHis: 0.889 ± 0.029
2.199IleIle: 2.199 ± 0.052
1.542IleLys: 1.542 ± 0.045
4.607IleLeu: 4.607 ± 0.08
1.018IleMet: 1.018 ± 0.032
1.414IleAsn: 1.414 ± 0.04
2.299IlePro: 2.299 ± 0.047
1.108IleGln: 1.108 ± 0.031
3.01IleArg: 3.01 ± 0.051
2.959IleSer: 2.959 ± 0.06
3.153IleThr: 3.153 ± 0.067
3.404IleVal: 3.404 ± 0.053
0.713IleTrp: 0.713 ± 0.027
1.096IleTyr: 1.096 ± 0.034
0.0IleXaa: 0.0 ± 0.0
Lys
4.586LysAla: 4.586 ± 0.085
0.193LysCys: 0.193 ± 0.014
1.745LysAsp: 1.745 ± 0.041
1.451LysGlu: 1.451 ± 0.046
0.96LysPhe: 0.96 ± 0.031
3.099LysGly: 3.099 ± 0.058
0.625LysHis: 0.625 ± 0.023
1.702LysIle: 1.702 ± 0.039
1.281LysLys: 1.281 ± 0.042
3.285LysLeu: 3.285 ± 0.059
0.95LysMet: 0.95 ± 0.032
0.827LysAsn: 0.827 ± 0.032
2.017LysPro: 2.017 ± 0.052
1.005LysGln: 1.005 ± 0.031
2.311LysArg: 2.311 ± 0.053
1.82LysSer: 1.82 ± 0.044
2.186LysThr: 2.186 ± 0.046
2.336LysVal: 2.336 ± 0.045
0.401LysTrp: 0.401 ± 0.022
0.643LysTyr: 0.643 ± 0.024
0.0LysXaa: 0.0 ± 0.0
Leu
14.152LeuAla: 14.152 ± 0.142
1.022LeuCys: 1.022 ± 0.035
5.636LeuAsp: 5.636 ± 0.078
5.1LeuGlu: 5.1 ± 0.079
3.583LeuPhe: 3.583 ± 0.071
8.88LeuGly: 8.88 ± 0.105
2.029LeuHis: 2.029 ± 0.044
5.303LeuIle: 5.303 ± 0.083
3.118LeuLys: 3.118 ± 0.061
9.33LeuLeu: 9.33 ± 0.128
2.764LeuMet: 2.764 ± 0.053
2.685LeuAsn: 2.685 ± 0.06
5.933LeuPro: 5.933 ± 0.086
2.844LeuGln: 2.844 ± 0.053
7.621LeuArg: 7.621 ± 0.096
6.356LeuSer: 6.356 ± 0.088
6.341LeuThr: 6.341 ± 0.107
6.721LeuVal: 6.721 ± 0.087
1.405LeuTrp: 1.405 ± 0.04
2.002LeuTyr: 2.002 ± 0.041
0.0LeuXaa: 0.0 ± 0.0
Met
3.74MetAla: 3.74 ± 0.071
0.163MetCys: 0.163 ± 0.013
1.397MetAsp: 1.397 ± 0.035
1.471MetGlu: 1.471 ± 0.038
0.827MetPhe: 0.827 ± 0.03
2.407MetGly: 2.407 ± 0.046
0.435MetHis: 0.435 ± 0.018
1.497MetIle: 1.497 ± 0.04
1.087MetLys: 1.087 ± 0.029
2.712MetLeu: 2.712 ± 0.055
0.824MetMet: 0.824 ± 0.03
0.815MetAsn: 0.815 ± 0.028
1.528MetPro: 1.528 ± 0.04
1.02MetGln: 1.02 ± 0.032
1.836MetArg: 1.836 ± 0.037
1.442MetSer: 1.442 ± 0.039
2.095MetThr: 2.095 ± 0.036
1.961MetVal: 1.961 ± 0.042
0.23MetTrp: 0.23 ± 0.015
0.337MetTyr: 0.337 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
3.234AsnAla: 3.234 ± 0.055
0.253AsnCys: 0.253 ± 0.015
1.569AsnAsp: 1.569 ± 0.083
1.043AsnGlu: 1.043 ± 0.03
0.943AsnPhe: 0.943 ± 0.028
2.484AsnGly: 2.484 ± 0.069
0.515AsnHis: 0.515 ± 0.023
1.306AsnIle: 1.306 ± 0.035
0.72AsnLys: 0.72 ± 0.025
2.597AsnLeu: 2.597 ± 0.053
0.678AsnMet: 0.678 ± 0.021
0.668AsnAsn: 0.668 ± 0.028
2.008AsnPro: 2.008 ± 0.044
0.723AsnGln: 0.723 ± 0.025
1.74AsnArg: 1.74 ± 0.043
1.234AsnSer: 1.234 ± 0.043
1.413AsnThr: 1.413 ± 0.051
1.621AsnVal: 1.621 ± 0.044
0.451AsnTrp: 0.451 ± 0.019
0.62AsnTyr: 0.62 ± 0.027
0.0AsnXaa: 0.0 ± 0.0
Pro
6.432ProAla: 6.432 ± 0.084
0.394ProCys: 0.394 ± 0.018
3.867ProAsp: 3.867 ± 0.066
3.906ProGlu: 3.906 ± 0.07
2.029ProPhe: 2.029 ± 0.047
4.472ProGly: 4.472 ± 0.068
1.064ProHis: 1.064 ± 0.03
2.267ProIle: 2.267 ± 0.041
1.879ProLys: 1.879 ± 0.046
4.891ProLeu: 4.891 ± 0.067
1.44ProMet: 1.44 ± 0.04
1.381ProAsn: 1.381 ± 0.04
2.418ProPro: 2.418 ± 0.059
1.942ProGln: 1.942 ± 0.045
2.827ProArg: 2.827 ± 0.054
2.505ProSer: 2.505 ± 0.049
2.432ProThr: 2.432 ± 0.045
4.142ProVal: 4.142 ± 0.065
0.663ProTrp: 0.663 ± 0.029
1.135ProTyr: 1.135 ± 0.031
0.0ProXaa: 0.0 ± 0.0
Gln
4.7GlnAla: 4.7 ± 0.077
0.222GlnCys: 0.222 ± 0.015
1.665GlnAsp: 1.665 ± 0.038
1.444GlnGlu: 1.444 ± 0.034
1.101GlnPhe: 1.101 ± 0.029
2.934GlnGly: 2.934 ± 0.053
0.609GlnHis: 0.609 ± 0.022
2.078GlnIle: 2.078 ± 0.038
1.151GlnLys: 1.151 ± 0.032
2.776GlnLeu: 2.776 ± 0.053
1.118GlnMet: 1.118 ± 0.03
0.973GlnAsn: 0.973 ± 0.026
1.747GlnPro: 1.747 ± 0.043
1.037GlnGln: 1.037 ± 0.037
2.279GlnArg: 2.279 ± 0.049
1.959GlnSer: 1.959 ± 0.039
1.958GlnThr: 1.958 ± 0.042
2.448GlnVal: 2.448 ± 0.051
0.424GlnTrp: 0.424 ± 0.019
0.569GlnTyr: 0.569 ± 0.022
0.0GlnXaa: 0.0 ± 0.0
Arg
8.714ArgAla: 8.714 ± 0.099
0.465ArgCys: 0.465 ± 0.022
3.965ArgAsp: 3.965 ± 0.057
3.298ArgGlu: 3.298 ± 0.067
2.718ArgPhe: 2.718 ± 0.051
4.627ArgGly: 4.627 ± 0.064
1.511ArgHis: 1.511 ± 0.04
3.618ArgIle: 3.618 ± 0.061
2.33ArgLys: 2.33 ± 0.05
7.394ArgLeu: 7.394 ± 0.097
2.023ArgMet: 2.023 ± 0.048
1.715ArgAsn: 1.715 ± 0.038
3.217ArgPro: 3.217 ± 0.054
2.185ArgGln: 2.185 ± 0.045
4.546ArgArg: 4.546 ± 0.075
3.189ArgSer: 3.189 ± 0.059
2.525ArgThr: 2.525 ± 0.046
4.62ArgVal: 4.62 ± 0.074
0.907ArgTrp: 0.907 ± 0.031
1.486ArgTyr: 1.486 ± 0.04
0.0ArgXaa: 0.0 ± 0.0
Ser
6.124SerAla: 6.124 ± 0.082
0.428SerCys: 0.428 ± 0.021
2.939SerAsp: 2.939 ± 0.063
2.423SerGlu: 2.423 ± 0.052
2.236SerPhe: 2.236 ± 0.048
5.304SerGly: 5.304 ± 0.103
1.054SerHis: 1.054 ± 0.033
2.298SerIle: 2.298 ± 0.048
1.549SerLys: 1.549 ± 0.04
5.26SerLeu: 5.26 ± 0.08
1.225SerMet: 1.225 ± 0.04
1.324SerAsn: 1.324 ± 0.035
2.559SerPro: 2.559 ± 0.049
1.547SerGln: 1.547 ± 0.04
3.006SerArg: 3.006 ± 0.055
2.458SerSer: 2.458 ± 0.053
2.5SerThr: 2.5 ± 0.058
3.721SerVal: 3.721 ± 0.058
0.654SerTrp: 0.654 ± 0.026
1.294SerTyr: 1.294 ± 0.035
0.0SerXaa: 0.0 ± 0.0
Thr
6.841ThrAla: 6.841 ± 0.103
0.5ThrCys: 0.5 ± 0.022
3.086ThrAsp: 3.086 ± 0.064
3.027ThrGlu: 3.027 ± 0.053
1.838ThrPhe: 1.838 ± 0.047
5.3ThrGly: 5.3 ± 0.136
1.064ThrHis: 1.064 ± 0.029
2.718ThrIle: 2.718 ± 0.071
1.621ThrLys: 1.621 ± 0.036
6.092ThrLeu: 6.092 ± 0.119
1.192ThrMet: 1.192 ± 0.035
1.377ThrAsn: 1.377 ± 0.051
3.562ThrPro: 3.562 ± 0.061
1.682ThrGln: 1.682 ± 0.036
3.439ThrArg: 3.439 ± 0.063
2.657ThrSer: 2.657 ± 0.062
2.952ThrThr: 2.952 ± 0.093
4.075ThrVal: 4.075 ± 0.109
0.651ThrTrp: 0.651 ± 0.025
1.244ThrTyr: 1.244 ± 0.036
0.0ThrXaa: 0.0 ± 0.0
Val
9.021ValAla: 9.021 ± 0.106
0.586ValCys: 0.586 ± 0.024
3.849ValAsp: 3.849 ± 0.061
4.242ValGlu: 4.242 ± 0.06
2.766ValPhe: 2.766 ± 0.052
5.162ValGly: 5.162 ± 0.068
1.161ValHis: 1.161 ± 0.033
4.156ValIle: 4.156 ± 0.058
2.308ValLys: 2.308 ± 0.057
7.618ValLeu: 7.618 ± 0.085
2.202ValMet: 2.202 ± 0.05
1.862ValAsn: 1.862 ± 0.055
3.452ValPro: 3.452 ± 0.06
2.324ValGln: 2.324 ± 0.046
3.678ValArg: 3.678 ± 0.066
3.801ValSer: 3.801 ± 0.068
4.738ValThr: 4.738 ± 0.084
5.364ValVal: 5.364 ± 0.081
0.958ValTrp: 0.958 ± 0.031
1.42ValTyr: 1.42 ± 0.033
0.0ValXaa: 0.0 ± 0.0
Trp
1.65TrpAla: 1.65 ± 0.039
0.135TrpCys: 0.135 ± 0.012
0.779TrpAsp: 0.779 ± 0.026
0.599TrpGlu: 0.599 ± 0.022
0.533TrpPhe: 0.533 ± 0.02
1.026TrpGly: 1.026 ± 0.028
0.349TrpHis: 0.349 ± 0.018
0.598TrpIle: 0.598 ± 0.023
0.446TrpLys: 0.446 ± 0.023
1.678TrpLeu: 1.678 ± 0.04
0.388TrpMet: 0.388 ± 0.019
0.415TrpAsn: 0.415 ± 0.02
0.734TrpPro: 0.734 ± 0.028
0.696TrpGln: 0.696 ± 0.027
1.091TrpArg: 1.091 ± 0.038
0.713TrpSer: 0.713 ± 0.026
0.669TrpThr: 0.669 ± 0.025
0.996TrpVal: 0.996 ± 0.033
0.214TrpTrp: 0.214 ± 0.014
0.278TrpTyr: 0.278 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.639TyrAla: 2.639 ± 0.046
0.255TyrCys: 0.255 ± 0.015
1.484TyrAsp: 1.484 ± 0.039
1.125TyrGlu: 1.125 ± 0.029
0.893TyrPhe: 0.893 ± 0.027
2.112TyrGly: 2.112 ± 0.054
0.51TyrHis: 0.51 ± 0.02
0.983TyrIle: 0.983 ± 0.034
0.619TyrLys: 0.619 ± 0.025
2.329TyrLeu: 2.329 ± 0.044
0.472TyrMet: 0.472 ± 0.019
0.615TyrAsn: 0.615 ± 0.026
1.034TyrPro: 1.034 ± 0.033
0.689TyrGln: 0.689 ± 0.025
1.531TyrArg: 1.531 ± 0.03
1.137TyrSer: 1.137 ± 0.03
1.09TyrThr: 1.09 ± 0.034
1.396TyrVal: 1.396 ± 0.035
0.362TyrTrp: 0.362 ± 0.018
0.544TyrTyr: 0.544 ± 0.02
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3709 proteins (1156015 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski