Arachidicoccus sp. B3-10
Average proteome isoelectric point is 6.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3921 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5P2G7F9|A0A5P2G7F9_9BACT Polyprenyl synthetase family protein OS=Arachidicoccus sp. B3-10 OX=2545455 GN=E0W69_013860 PE=3 SV=1
MM1 pKa = 7.47 VKK3 pKa = 9.28 FTVEE7 pKa = 4.28 DD8 pKa = 3.65 RR9 pKa = 11.84 DD10 pKa = 3.93 GSQQEE15 pKa = 4.59 LEE17 pKa = 4.73 APDD20 pKa = 5.22 DD21 pKa = 3.57 MGLNLMEE28 pKa = 4.09 TLKK31 pKa = 10.96 AFEE34 pKa = 4.44 YY35 pKa = 10.63 EE36 pKa = 4.25 VLATCGGMALCGTCHH51 pKa = 5.96 VKK53 pKa = 10.62 VLAGGEE59 pKa = 4.01 NLPEE63 pKa = 4.18 QGDD66 pKa = 4.16 AEE68 pKa = 5.39 LDD70 pKa = 3.79 MLDD73 pKa = 4.89 MIPDD77 pKa = 3.72 SEE79 pKa = 4.92 SNSRR83 pKa = 11.84 LSCQLQVNDD92 pKa = 4.19 NLEE95 pKa = 4.05 GCTFQICGSLSEE107 pKa = 4.54 DD108 pKa = 3.23
Molecular weight: 11.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.737
IPC2_protein 3.77
IPC_protein 3.719
Toseland 3.528
ProMoST 3.808
Dawson 3.694
Bjellqvist 3.897
Wikipedia 3.617
Rodwell 3.554
Grimsley 3.439
Solomon 3.681
Lehninger 3.63
Nozaki 3.821
DTASelect 3.986
Thurlkill 3.579
EMBOSS 3.63
Sillero 3.834
Patrickios 0.846
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.784
Protein with the highest isoelectric point:
>tr|A0A5P2G5N2|A0A5P2G5N2_9BACT BamA/TamA family outer membrane protein OS=Arachidicoccus sp. B3-10 OX=2545455 GN=E0W69_020070 PE=4 SV=1
MM1 pKa = 7.38 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPHH8 pKa = 3.7 NRR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.26 SIHH16 pKa = 5.24 GFRR19 pKa = 11.84 EE20 pKa = 3.88 RR21 pKa = 11.84 MSTANGRR28 pKa = 11.84 KK29 pKa = 8.58 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.08 GRR39 pKa = 11.84 KK40 pKa = 8.3 KK41 pKa = 10.08 LTVSSEE47 pKa = 4.04 KK48 pKa = 9.88 TLKK51 pKa = 10.65
Molecular weight: 6.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.328
IPC2_protein 10.701
IPC_protein 12.266
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.223
Grimsley 12.486
Solomon 12.925
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 11.959
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.077
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3921
0
3921
1376694
26
2127
351.1
39.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.447 ± 0.034
0.779 ± 0.013
5.364 ± 0.028
5.537 ± 0.048
5.064 ± 0.028
6.318 ± 0.038
1.844 ± 0.016
8.143 ± 0.039
7.596 ± 0.04
9.291 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.243 ± 0.017
6.542 ± 0.044
3.55 ± 0.019
3.843 ± 0.022
3.336 ± 0.024
6.967 ± 0.039
5.726 ± 0.034
5.727 ± 0.032
1.236 ± 0.014
4.447 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here