SAR202 cluster bacterium AD-493-K16_JPT_193m

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Chloroflexi; Chloroflexi incertae sedis; SAR202 cluster

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 268 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5N8YZ52|A0A5N8YZ52_9CHLR Molybdopterin synthase sulfur carrier subunit OS=SAR202 cluster bacterium AD-493-K16_JPT_193m OX=2587841 GN=moaD PE=3 SV=1
MM1 pKa = 7.6SEE3 pKa = 3.7QSEE6 pKa = 4.32PFQRR10 pKa = 11.84ISLEE14 pKa = 4.01EE15 pKa = 4.05AYY17 pKa = 9.76KK18 pKa = 10.68LYY20 pKa = 10.63EE21 pKa = 4.28QGDD24 pKa = 4.13STVVDD29 pKa = 3.71VRR31 pKa = 11.84QLDD34 pKa = 3.67EE35 pKa = 4.18WNAGHH40 pKa = 5.62VTGAIHH46 pKa = 7.1IPVEE50 pKa = 4.57DD51 pKa = 4.94IISQVDD57 pKa = 3.6TLPNDD62 pKa = 3.52SNLLFICAAGVRR74 pKa = 11.84SALACEE80 pKa = 4.2MAAAMGRR87 pKa = 11.84DD88 pKa = 3.4SSILFNIEE96 pKa = 4.28DD97 pKa = 4.08GTGAWIDD104 pKa = 3.2QGYY107 pKa = 6.2PTTYY111 pKa = 9.41EE112 pKa = 3.85TDD114 pKa = 3.11SS115 pKa = 3.47

Molecular weight: 12.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5N8YYN9|A0A5N8YYN9_9CHLR Thiamine pyrophosphate-binding protein OS=SAR202 cluster bacterium AD-493-K16_JPT_193m OX=2587841 GN=FIL93_00685 PE=4 SV=1
MM1 pKa = 7.28GRR3 pKa = 11.84YY4 pKa = 7.15STRR7 pKa = 11.84SSGRR11 pKa = 11.84KK12 pKa = 8.54LRR14 pKa = 11.84HH15 pKa = 4.4IRR17 pKa = 11.84VRR19 pKa = 11.84KK20 pKa = 9.53KK21 pKa = 10.52VVGTAEE27 pKa = 4.19RR28 pKa = 11.84PRR30 pKa = 11.84LTVFRR35 pKa = 11.84SSRR38 pKa = 11.84QVYY41 pKa = 9.33AQLIDD46 pKa = 4.31DD47 pKa = 4.42SLGHH51 pKa = 5.82TMASASSLPLKK62 pKa = 10.25EE63 pKa = 4.72DD64 pKa = 3.3YY65 pKa = 11.02KK66 pKa = 11.22EE67 pKa = 3.95ATKK70 pKa = 10.65TNTAAVVGKK79 pKa = 9.91NIAEE83 pKa = 4.22AAVKK87 pKa = 10.4LGVSRR92 pKa = 11.84IVFDD96 pKa = 3.72RR97 pKa = 11.84GGYY100 pKa = 9.16KK101 pKa = 9.09YY102 pKa = 10.17HH103 pKa = 6.55GRR105 pKa = 11.84IKK107 pKa = 10.75ALAEE111 pKa = 3.82AVRR114 pKa = 11.84EE115 pKa = 4.2GGLVFF120 pKa = 5.43

Molecular weight: 13.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

268

0

268

86820

37

1511

324.0

35.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.179 ± 0.133

0.79 ± 0.043

5.225 ± 0.113

6.062 ± 0.135

3.966 ± 0.098

8.349 ± 0.117

1.963 ± 0.057

7.352 ± 0.105

4.585 ± 0.102

10.043 ± 0.167

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.579 ± 0.063

3.868 ± 0.078

4.489 ± 0.078

2.906 ± 0.086

5.01 ± 0.102

7.175 ± 0.115

5.487 ± 0.1

7.845 ± 0.128

1.279 ± 0.067

2.85 ± 0.081
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski