Microbacterium sp. SA39

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium; unclassified Microbacterium

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3732 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F2C2X1|A0A0F2C2X1_9MICO Uncharacterized protein OS=Microbacterium sp. SA39 OX=1263625 GN=RS85_03298 PE=4 SV=1
MM1 pKa = 7.61AGAALPAAAEE11 pKa = 4.26SPEE14 pKa = 4.24FGPSVQDD21 pKa = 3.41GTIGSNKK28 pKa = 10.0DD29 pKa = 3.07AGSLNEE35 pKa = 4.51CPADD39 pKa = 3.52QALVGVRR46 pKa = 11.84VEE48 pKa = 4.87DD49 pKa = 3.67RR50 pKa = 11.84TNAPGDD56 pKa = 3.59VAGGIINDD64 pKa = 3.65FDD66 pKa = 4.7LLCGTIEE73 pKa = 4.23VNSPSAITVAQSPSYY88 pKa = 11.1LDD90 pKa = 3.38FPSYY94 pKa = 11.61VNGDD98 pKa = 3.84GVFHH102 pKa = 7.11VATCPANMVVTQMGGHH118 pKa = 6.67FFQGQGFPWASTIQISCQPLRR139 pKa = 11.84FDD141 pKa = 3.08AQGRR145 pKa = 11.84IRR147 pKa = 11.84VDD149 pKa = 3.3TTATPVALLAGDD161 pKa = 4.22NEE163 pKa = 4.53NLNAPAGFNGPFCGADD179 pKa = 3.13DD180 pKa = 3.93TQIVRR185 pKa = 11.84GYY187 pKa = 9.25RR188 pKa = 11.84PQFGGEE194 pKa = 4.39GYY196 pKa = 10.76DD197 pKa = 4.38GINVSCASLASDD209 pKa = 4.99FGDD212 pKa = 5.02APAQYY217 pKa = 9.01PAAGFEE223 pKa = 4.46INGATFVGEE232 pKa = 4.35SVDD235 pKa = 4.51AEE237 pKa = 4.23PAEE240 pKa = 4.3QPSADD245 pKa = 3.37ASADD249 pKa = 3.57GVSDD253 pKa = 4.69DD254 pKa = 4.4GVTFPTIIGGVTPSAPVNVEE274 pKa = 3.7VTNFAPVPATLTGWIDD290 pKa = 3.7FDD292 pKa = 3.66QDD294 pKa = 3.59GVFEE298 pKa = 4.94AGEE301 pKa = 4.18QVTAPVAAGFTGPVVLTFGDD321 pKa = 3.7VAARR325 pKa = 11.84TTAAGGTTTARR336 pKa = 11.84FTLVVNGVAGEE347 pKa = 4.36VEE349 pKa = 4.18DD350 pKa = 4.06HH351 pKa = 6.03QVIITPQAPALTIVKK366 pKa = 10.47ASTTTAASAAGQEE379 pKa = 3.7IDD381 pKa = 3.48YY382 pKa = 9.26TFAVTNTGNVTISNIEE398 pKa = 3.75ITDD401 pKa = 3.71AQLDD405 pKa = 4.03APAVCDD411 pKa = 5.39DD412 pKa = 3.75VVLDD416 pKa = 4.73PGAATDD422 pKa = 3.75CTGLHH427 pKa = 5.79TVTQAEE433 pKa = 4.11IDD435 pKa = 3.7GGDD438 pKa = 3.7VIEE441 pKa = 5.45NIATATGTPPGSTTPIPPTTSNPVIIPTVQTPALTIVKK479 pKa = 10.39ASTTTAASAAGQQIDD494 pKa = 3.98YY495 pKa = 9.21TLSATNTGNVTISNVAVSDD514 pKa = 3.95AQLDD518 pKa = 4.2TPAVCDD524 pKa = 3.83EE525 pKa = 4.32VVLAPGAVTEE535 pKa = 4.36CTGVHH540 pKa = 5.44TVTQAEE546 pKa = 4.34IEE548 pKa = 4.15GGEE551 pKa = 4.23IVNTATVTGTPPGSTTPIPPATSNPVIIPTAQTPALTIDD590 pKa = 3.86KK591 pKa = 9.57SASSSSVQQGATITYY606 pKa = 9.6LFVARR611 pKa = 11.84NTGNVTVSNVTITDD625 pKa = 4.18PLPGLSALDD634 pKa = 4.18CSTPAPVTLAVGEE647 pKa = 4.47TLTCTANYY655 pKa = 7.18VTTAADD661 pKa = 3.45GRR663 pKa = 11.84AGSVLNTATVSGDD676 pKa = 3.7DD677 pKa = 4.12PSGQPVGASDD687 pKa = 3.66RR688 pKa = 11.84VDD690 pKa = 3.16VTVIPQAAEE699 pKa = 3.68AAGLANTGSEE709 pKa = 4.18STLPFAAFGVLLLLVGTLIVVRR731 pKa = 11.84RR732 pKa = 11.84RR733 pKa = 11.84VAAII737 pKa = 3.42

Molecular weight: 73.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F2C4N2|A0A0F2C4N2_9MICO Glyco_tran_28_C domain-containing protein OS=Microbacterium sp. SA39 OX=1263625 GN=RS85_03518 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3732

0

3732

1183266

29

2441

317.1

33.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.315 ± 0.055

0.463 ± 0.009

6.329 ± 0.036

5.9 ± 0.04

3.21 ± 0.026

8.812 ± 0.036

1.97 ± 0.021

4.87 ± 0.028

1.921 ± 0.024

10.083 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.855 ± 0.017

1.955 ± 0.022

5.263 ± 0.031

2.779 ± 0.022

7.231 ± 0.042

5.732 ± 0.027

6.048 ± 0.037

8.815 ± 0.039

1.522 ± 0.019

1.929 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski