Amino acid dipepetide frequency for Microbacterium sp. SA39

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.971AlaAla: 19.971 ± 0.192
0.597AlaCys: 0.597 ± 0.026
8.505AlaAsp: 8.505 ± 0.113
8.669AlaGlu: 8.669 ± 0.109
4.106AlaPhe: 4.106 ± 0.064
11.67AlaGly: 11.67 ± 0.11
2.447AlaHis: 2.447 ± 0.053
6.021AlaIle: 6.021 ± 0.075
2.583AlaLys: 2.583 ± 0.059
14.108AlaLeu: 14.108 ± 0.137
2.672AlaMet: 2.672 ± 0.05
2.235AlaAsn: 2.235 ± 0.044
6.31AlaPro: 6.31 ± 0.107
3.772AlaGln: 3.772 ± 0.064
8.782AlaArg: 8.782 ± 0.105
7.438AlaSer: 7.438 ± 0.088
7.27AlaThr: 7.27 ± 0.093
11.745AlaVal: 11.745 ± 0.115
1.84AlaTrp: 1.84 ± 0.043
2.412AlaTyr: 2.412 ± 0.043
0.0AlaXaa: 0.0 ± 0.0
Cys
0.594CysAla: 0.594 ± 0.027
0.047CysCys: 0.047 ± 0.007
0.299CysAsp: 0.299 ± 0.015
0.253CysGlu: 0.253 ± 0.016
0.18CysPhe: 0.18 ± 0.012
0.501CysGly: 0.501 ± 0.024
0.109CysHis: 0.109 ± 0.012
0.204CysIle: 0.204 ± 0.013
0.053CysLys: 0.053 ± 0.007
0.393CysLeu: 0.393 ± 0.018
0.075CysMet: 0.075 ± 0.009
0.089CysAsn: 0.089 ± 0.008
0.251CysPro: 0.251 ± 0.017
0.114CysGln: 0.114 ± 0.01
0.309CysArg: 0.309 ± 0.019
0.293CysSer: 0.293 ± 0.017
0.314CysThr: 0.314 ± 0.016
0.391CysVal: 0.391 ± 0.018
0.066CysTrp: 0.066 ± 0.006
0.091CysTyr: 0.091 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
9.7AspAla: 9.7 ± 0.107
0.237AspCys: 0.237 ± 0.017
4.542AspAsp: 4.542 ± 0.075
4.543AspGlu: 4.543 ± 0.065
1.774AspPhe: 1.774 ± 0.043
6.043AspGly: 6.043 ± 0.085
1.131AspHis: 1.131 ± 0.031
2.663AspIle: 2.663 ± 0.055
0.959AspLys: 0.959 ± 0.032
6.256AspLeu: 6.256 ± 0.081
0.876AspMet: 0.876 ± 0.026
0.967AspAsn: 0.967 ± 0.03
4.035AspPro: 4.035 ± 0.065
1.578AspGln: 1.578 ± 0.035
4.577AspArg: 4.577 ± 0.062
2.768AspSer: 2.768 ± 0.049
2.898AspThr: 2.898 ± 0.058
5.552AspVal: 5.552 ± 0.077
0.936AspTrp: 0.936 ± 0.033
1.256AspTyr: 1.256 ± 0.035
0.0AspXaa: 0.0 ± 0.0
Glu
7.162GluAla: 7.162 ± 0.095
0.238GluCys: 0.238 ± 0.014
2.913GluAsp: 2.913 ± 0.05
3.377GluGlu: 3.377 ± 0.061
1.827GluPhe: 1.827 ± 0.04
4.414GluGly: 4.414 ± 0.073
1.443GluHis: 1.443 ± 0.036
3.143GluIle: 3.143 ± 0.067
1.587GluLys: 1.587 ± 0.044
6.447GluLeu: 6.447 ± 0.085
1.012GluMet: 1.012 ± 0.027
1.411GluAsn: 1.411 ± 0.04
3.086GluPro: 3.086 ± 0.065
2.247GluGln: 2.247 ± 0.047
5.232GluArg: 5.232 ± 0.07
3.179GluSer: 3.179 ± 0.058
3.385GluThr: 3.385 ± 0.064
4.736GluVal: 4.736 ± 0.067
0.919GluTrp: 0.919 ± 0.029
1.238GluTyr: 1.238 ± 0.036
0.0GluXaa: 0.0 ± 0.0
Phe
4.412PheAla: 4.412 ± 0.054
0.161PheCys: 0.161 ± 0.013
2.509PheAsp: 2.509 ± 0.05
1.762PheGlu: 1.762 ± 0.039
1.113PhePhe: 1.113 ± 0.036
3.495PheGly: 3.495 ± 0.056
0.576PheHis: 0.576 ± 0.023
1.304PheIle: 1.304 ± 0.037
0.436PheLys: 0.436 ± 0.019
2.977PheLeu: 2.977 ± 0.05
0.465PheMet: 0.465 ± 0.019
0.663PheAsn: 0.663 ± 0.024
1.51PhePro: 1.51 ± 0.038
0.805PheGln: 0.805 ± 0.027
1.903PheArg: 1.903 ± 0.041
1.798PheSer: 1.798 ± 0.037
2.207PheThr: 2.207 ± 0.043
2.844PheVal: 2.844 ± 0.053
0.529PheTrp: 0.529 ± 0.022
0.625PheTyr: 0.625 ± 0.021
0.0PheXaa: 0.0 ± 0.0
Gly
10.68GlyAla: 10.68 ± 0.115
0.528GlyCys: 0.528 ± 0.025
5.109GlyAsp: 5.109 ± 0.073
5.209GlyGlu: 5.209 ± 0.077
3.326GlyPhe: 3.326 ± 0.054
7.444GlyGly: 7.444 ± 0.106
1.795GlyHis: 1.795 ± 0.043
5.165GlyIle: 5.165 ± 0.068
1.985GlyLys: 1.985 ± 0.042
8.462GlyLeu: 8.462 ± 0.1
2.029GlyMet: 2.029 ± 0.048
1.7GlyAsn: 1.7 ± 0.042
3.652GlyPro: 3.652 ± 0.06
2.336GlyGln: 2.336 ± 0.044
6.12GlyArg: 6.12 ± 0.078
5.335GlySer: 5.335 ± 0.063
5.519GlyThr: 5.519 ± 0.088
7.857GlyVal: 7.857 ± 0.095
1.672GlyTrp: 1.672 ± 0.041
2.195GlyTyr: 2.195 ± 0.052
0.0GlyXaa: 0.0 ± 0.0
His
2.35HisAla: 2.35 ± 0.047
0.099HisCys: 0.099 ± 0.009
1.34HisAsp: 1.34 ± 0.036
1.19HisGlu: 1.19 ± 0.036
0.574HisPhe: 0.574 ± 0.024
1.951HisGly: 1.951 ± 0.044
0.565HisHis: 0.565 ± 0.024
0.751HisIle: 0.751 ± 0.026
0.272HisLys: 0.272 ± 0.016
2.036HisLeu: 2.036 ± 0.045
0.319HisMet: 0.319 ± 0.016
0.325HisAsn: 0.325 ± 0.018
1.514HisPro: 1.514 ± 0.037
0.473HisGln: 0.473 ± 0.02
1.632HisArg: 1.632 ± 0.033
0.971HisSer: 0.971 ± 0.033
0.975HisThr: 0.975 ± 0.03
1.651HisVal: 1.651 ± 0.039
0.31HisTrp: 0.31 ± 0.017
0.397HisTyr: 0.397 ± 0.017
0.0HisXaa: 0.0 ± 0.0
Ile
7.546IleAla: 7.546 ± 0.083
0.254IleCys: 0.254 ± 0.016
3.823IleAsp: 3.823 ± 0.059
3.089IleGlu: 3.089 ± 0.053
1.266IlePhe: 1.266 ± 0.035
4.901IleGly: 4.901 ± 0.073
0.765IleHis: 0.765 ± 0.028
2.151IleIle: 2.151 ± 0.044
0.718IleLys: 0.718 ± 0.025
4.122IleLeu: 4.122 ± 0.067
0.652IleMet: 0.652 ± 0.023
0.901IleAsn: 0.901 ± 0.029
2.581IlePro: 2.581 ± 0.049
1.003IleGln: 1.003 ± 0.032
3.131IleArg: 3.131 ± 0.051
2.519IleSer: 2.519 ± 0.046
2.946IleThr: 2.946 ± 0.046
4.971IleVal: 4.971 ± 0.071
0.586IleTrp: 0.586 ± 0.023
0.772IleTyr: 0.772 ± 0.028
0.0IleXaa: 0.0 ± 0.0
Lys
2.377LysAla: 2.377 ± 0.057
0.052LysCys: 0.052 ± 0.006
1.109LysAsp: 1.109 ± 0.033
1.029LysGlu: 1.029 ± 0.032
0.465LysPhe: 0.465 ± 0.018
1.498LysGly: 1.498 ± 0.047
0.434LysHis: 0.434 ± 0.02
0.927LysIle: 0.927 ± 0.032
0.761LysLys: 0.761 ± 0.034
1.799LysLeu: 1.799 ± 0.042
0.399LysMet: 0.399 ± 0.02
0.509LysAsn: 0.509 ± 0.024
1.108LysPro: 1.108 ± 0.037
0.646LysGln: 0.646 ± 0.024
1.424LysArg: 1.424 ± 0.038
1.121LysSer: 1.121 ± 0.033
1.272LysThr: 1.272 ± 0.039
1.622LysVal: 1.622 ± 0.046
0.247LysTrp: 0.247 ± 0.012
0.408LysTyr: 0.408 ± 0.021
0.0LysXaa: 0.0 ± 0.0
Leu
14.184LeuAla: 14.184 ± 0.134
0.478LeuCys: 0.478 ± 0.021
6.621LeuAsp: 6.621 ± 0.079
5.171LeuGlu: 5.171 ± 0.074
3.02LeuPhe: 3.02 ± 0.056
9.024LeuGly: 9.024 ± 0.111
1.96LeuHis: 1.96 ± 0.049
4.882LeuIle: 4.882 ± 0.082
1.643LeuLys: 1.643 ± 0.05
10.38LeuLeu: 10.38 ± 0.149
1.708LeuMet: 1.708 ± 0.037
1.822LeuAsn: 1.822 ± 0.044
5.453LeuPro: 5.453 ± 0.07
2.659LeuGln: 2.659 ± 0.046
7.72LeuArg: 7.72 ± 0.1
6.046LeuSer: 6.046 ± 0.066
6.254LeuThr: 6.254 ± 0.076
8.896LeuVal: 8.896 ± 0.109
1.311LeuTrp: 1.311 ± 0.038
1.602LeuTyr: 1.602 ± 0.039
0.0LeuXaa: 0.0 ± 0.0
Met
2.063MetAla: 2.063 ± 0.036
0.088MetCys: 0.088 ± 0.009
0.813MetAsp: 0.813 ± 0.028
0.69MetGlu: 0.69 ± 0.024
0.562MetPhe: 0.562 ± 0.026
1.273MetGly: 1.273 ± 0.035
0.338MetHis: 0.338 ± 0.016
0.95MetIle: 0.95 ± 0.031
0.462MetLys: 0.462 ± 0.02
2.14MetLeu: 2.14 ± 0.045
0.339MetMet: 0.339 ± 0.018
0.548MetAsn: 0.548 ± 0.022
1.187MetPro: 1.187 ± 0.036
0.561MetGln: 0.561 ± 0.023
1.447MetArg: 1.447 ± 0.035
1.533MetSer: 1.533 ± 0.038
1.768MetThr: 1.768 ± 0.039
1.324MetVal: 1.324 ± 0.036
0.214MetTrp: 0.214 ± 0.013
0.249MetTyr: 0.249 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
2.493AsnAla: 2.493 ± 0.045
0.101AsnCys: 0.101 ± 0.008
1.184AsnAsp: 1.184 ± 0.033
1.048AsnGlu: 1.048 ± 0.033
0.644AsnPhe: 0.644 ± 0.028
1.926AsnGly: 1.926 ± 0.057
0.374AsnHis: 0.374 ± 0.02
0.882AsnIle: 0.882 ± 0.029
0.374AsnLys: 0.374 ± 0.02
1.881AsnLeu: 1.881 ± 0.039
0.336AsnMet: 0.336 ± 0.016
0.461AsnAsn: 0.461 ± 0.018
1.538AsnPro: 1.538 ± 0.038
0.521AsnGln: 0.521 ± 0.023
1.238AsnArg: 1.238 ± 0.035
1.015AsnSer: 1.015 ± 0.033
1.134AsnThr: 1.134 ± 0.039
1.643AsnVal: 1.643 ± 0.043
0.309AsnTrp: 0.309 ± 0.016
0.45AsnTyr: 0.45 ± 0.019
0.0AsnXaa: 0.0 ± 0.0
Pro
6.752ProAla: 6.752 ± 0.098
0.17ProCys: 0.17 ± 0.012
3.756ProAsp: 3.756 ± 0.056
4.052ProGlu: 4.052 ± 0.061
1.792ProPhe: 1.792 ± 0.037
4.843ProGly: 4.843 ± 0.062
1.091ProHis: 1.091 ± 0.032
2.131ProIle: 2.131 ± 0.046
0.958ProLys: 0.958 ± 0.032
5.068ProLeu: 5.068 ± 0.074
0.863ProMet: 0.863 ± 0.027
0.971ProAsn: 0.971 ± 0.029
2.209ProPro: 2.209 ± 0.056
1.596ProGln: 1.596 ± 0.036
3.348ProArg: 3.348 ± 0.06
3.129ProSer: 3.129 ± 0.049
3.257ProThr: 3.257 ± 0.061
4.769ProVal: 4.769 ± 0.063
0.864ProTrp: 0.864 ± 0.034
1.007ProTyr: 1.007 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
3.407GlnAla: 3.407 ± 0.064
0.101GlnCys: 0.101 ± 0.009
1.368GlnAsp: 1.368 ± 0.035
1.36GlnGlu: 1.36 ± 0.039
0.849GlnPhe: 0.849 ± 0.029
2.131GlnGly: 2.131 ± 0.045
0.636GlnHis: 0.636 ± 0.021
1.455GlnIle: 1.455 ± 0.039
0.682GlnLys: 0.682 ± 0.024
3.084GlnLeu: 3.084 ± 0.056
0.522GlnMet: 0.522 ± 0.023
0.687GlnAsn: 0.687 ± 0.026
1.455GlnPro: 1.455 ± 0.034
1.155GlnGln: 1.155 ± 0.036
2.392GlnArg: 2.392 ± 0.049
1.509GlnSer: 1.509 ± 0.034
1.576GlnThr: 1.576 ± 0.036
2.365GlnVal: 2.365 ± 0.044
0.455GlnTrp: 0.455 ± 0.017
0.599GlnTyr: 0.599 ± 0.027
0.0GlnXaa: 0.0 ± 0.0
Arg
8.76ArgAla: 8.76 ± 0.107
0.281ArgCys: 0.281 ± 0.018
4.372ArgAsp: 4.372 ± 0.069
4.422ArgGlu: 4.422 ± 0.063
2.433ArgPhe: 2.433 ± 0.044
5.496ArgGly: 5.496 ± 0.081
1.552ArgHis: 1.552 ± 0.044
3.817ArgIle: 3.817 ± 0.066
1.328ArgLys: 1.328 ± 0.038
7.217ArgLeu: 7.217 ± 0.099
1.871ArgMet: 1.871 ± 0.043
1.357ArgAsn: 1.357 ± 0.037
3.511ArgPro: 3.511 ± 0.061
2.045ArgGln: 2.045 ± 0.044
6.533ArgArg: 6.533 ± 0.091
4.338ArgSer: 4.338 ± 0.067
4.267ArgThr: 4.267 ± 0.062
6.016ArgVal: 6.016 ± 0.063
1.208ArgTrp: 1.208 ± 0.033
1.492ArgTyr: 1.492 ± 0.036
0.0ArgXaa: 0.0 ± 0.0
Ser
7.391SerAla: 7.391 ± 0.091
0.262SerCys: 0.262 ± 0.016
3.457SerAsp: 3.457 ± 0.059
3.004SerGlu: 3.004 ± 0.053
2.038SerPhe: 2.038 ± 0.045
5.838SerGly: 5.838 ± 0.07
1.029SerHis: 1.029 ± 0.026
2.897SerIle: 2.897 ± 0.048
1.109SerLys: 1.109 ± 0.032
5.44SerLeu: 5.44 ± 0.069
1.269SerMet: 1.269 ± 0.034
1.097SerAsn: 1.097 ± 0.032
3.078SerPro: 3.078 ± 0.051
1.307SerGln: 1.307 ± 0.039
3.927SerArg: 3.927 ± 0.067
3.585SerSer: 3.585 ± 0.058
3.715SerThr: 3.715 ± 0.058
4.796SerVal: 4.796 ± 0.068
0.96SerTrp: 0.96 ± 0.03
1.115SerTyr: 1.115 ± 0.031
0.0SerXaa: 0.0 ± 0.0
Thr
7.865ThrAla: 7.865 ± 0.098
0.256ThrCys: 0.256 ± 0.017
3.71ThrAsp: 3.71 ± 0.06
3.228ThrGlu: 3.228 ± 0.059
1.893ThrPhe: 1.893 ± 0.045
5.563ThrGly: 5.563 ± 0.07
1.132ThrHis: 1.132 ± 0.031
3.027ThrIle: 3.027 ± 0.063
1.168ThrLys: 1.168 ± 0.037
6.038ThrLeu: 6.038 ± 0.069
1.006ThrMet: 1.006 ± 0.026
1.149ThrAsn: 1.149 ± 0.034
3.866ThrPro: 3.866 ± 0.07
1.545ThrGln: 1.545 ± 0.037
3.798ThrArg: 3.798 ± 0.056
3.447ThrSer: 3.447 ± 0.061
3.922ThrThr: 3.922 ± 0.077
5.802ThrVal: 5.802 ± 0.088
0.898ThrTrp: 0.898 ± 0.028
1.168ThrTyr: 1.168 ± 0.035
0.0ThrXaa: 0.0 ± 0.0
Val
11.418ValAla: 11.418 ± 0.111
0.481ValCys: 0.481 ± 0.022
5.714ValAsp: 5.714 ± 0.077
5.051ValGlu: 5.051 ± 0.072
2.949ValPhe: 2.949 ± 0.055
7.162ValGly: 7.162 ± 0.082
1.711ValHis: 1.711 ± 0.043
4.816ValIle: 4.816 ± 0.076
1.524ValLys: 1.524 ± 0.036
9.173ValLeu: 9.173 ± 0.101
1.522ValMet: 1.522 ± 0.038
1.761ValAsn: 1.761 ± 0.039
4.599ValPro: 4.599 ± 0.067
2.297ValGln: 2.297 ± 0.037
6.005ValArg: 6.005 ± 0.082
5.181ValSer: 5.181 ± 0.072
5.663ValThr: 5.663 ± 0.088
8.445ValVal: 8.445 ± 0.102
1.201ValTrp: 1.201 ± 0.032
1.48ValTyr: 1.48 ± 0.036
0.0ValXaa: 0.0 ± 0.0
Trp
1.64TrpAla: 1.64 ± 0.04
0.094TrpCys: 0.094 ± 0.009
0.834TrpAsp: 0.834 ± 0.028
0.715TrpGlu: 0.715 ± 0.028
0.577TrpPhe: 0.577 ± 0.025
1.133TrpGly: 1.133 ± 0.033
0.33TrpHis: 0.33 ± 0.018
0.749TrpIle: 0.749 ± 0.021
0.295TrpLys: 0.295 ± 0.015
1.759TrpLeu: 1.759 ± 0.042
0.362TrpMet: 0.362 ± 0.019
0.45TrpAsn: 0.45 ± 0.017
0.694TrpPro: 0.694 ± 0.023
0.602TrpGln: 0.602 ± 0.022
1.205TrpArg: 1.205 ± 0.035
0.976TrpSer: 0.976 ± 0.031
0.967TrpThr: 0.967 ± 0.031
1.198TrpVal: 1.198 ± 0.033
0.365TrpTrp: 0.365 ± 0.019
0.274TrpTyr: 0.274 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.39TyrAla: 2.39 ± 0.043
0.102TyrCys: 0.102 ± 0.009
1.31TyrAsp: 1.31 ± 0.04
1.137TyrGlu: 1.137 ± 0.035
0.706TyrPhe: 0.706 ± 0.023
1.809TyrGly: 1.809 ± 0.041
0.279TyrHis: 0.279 ± 0.017
0.759TyrIle: 0.759 ± 0.026
0.312TyrLys: 0.312 ± 0.016
2.054TyrLeu: 2.054 ± 0.04
0.252TyrMet: 0.252 ± 0.015
0.45TyrAsn: 0.45 ± 0.022
0.99TyrPro: 0.99 ± 0.032
0.528TyrGln: 0.528 ± 0.019
1.591TyrArg: 1.591 ± 0.038
1.133TyrSer: 1.133 ± 0.029
1.17TyrThr: 1.17 ± 0.034
1.531TyrVal: 1.531 ± 0.036
0.328TyrTrp: 0.328 ± 0.015
0.46TyrTyr: 0.46 ± 0.019
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3732 proteins (1183266 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski