Holospora curviuscula

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Holosporales; Holosporaceae; Holospora

Average proteome isoelectric point is 7.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1546 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S5R9S0|A0A2S5R9S0_9PROT Elongation factor G OS=Holospora curviuscula OX=1082868 GN=fusA PE=3 SV=1
MM1 pKa = 7.54CAFHH5 pKa = 7.44PLQKK9 pKa = 10.33LLYY12 pKa = 8.99VITIFGSSVLAEE24 pKa = 4.48NILPPEE30 pKa = 4.36NSVSTPNNLAAPLPLEE46 pKa = 4.77SNSEE50 pKa = 4.45TNSPPPDD57 pKa = 3.27NEE59 pKa = 4.41TYY61 pKa = 10.62EE62 pKa = 4.22EE63 pKa = 4.32EE64 pKa = 4.36PLSKK68 pKa = 10.29EE69 pKa = 3.85AQQEE73 pKa = 4.16KK74 pKa = 10.47EE75 pKa = 4.36LYY77 pKa = 10.55DD78 pKa = 3.87FNAYY82 pKa = 9.58FDD84 pKa = 4.7NYY86 pKa = 9.33MDD88 pKa = 4.5PRR90 pKa = 11.84PFVIGSTEE98 pKa = 3.66FGTT101 pKa = 4.29

Molecular weight: 11.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S5RE15|A0A2S5RE15_9PROT Uncharacterized protein OS=Holospora curviuscula OX=1082868 GN=HCUR_00196 PE=4 SV=1
MM1 pKa = 7.25ARR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84SEE8 pKa = 3.68KK9 pKa = 10.42RR10 pKa = 11.84QIAPDD15 pKa = 3.57LRR17 pKa = 11.84YY18 pKa = 9.94EE19 pKa = 4.28DD20 pKa = 4.08SVVAKK25 pKa = 9.22FINCLMWKK33 pKa = 9.3GKK35 pKa = 8.21KK36 pKa = 8.49TIAQNIVYY44 pKa = 9.86GALDD48 pKa = 3.37RR49 pKa = 11.84AAAKK53 pKa = 9.67LQKK56 pKa = 10.36DD57 pKa = 3.61PRR59 pKa = 11.84EE60 pKa = 4.27LFHH63 pKa = 7.16SALGNVRR70 pKa = 11.84PNVEE74 pKa = 3.39VRR76 pKa = 11.84ARR78 pKa = 11.84RR79 pKa = 11.84VGGATYY85 pKa = 8.12TVPVAVSVDD94 pKa = 3.17RR95 pKa = 11.84GQILAVRR102 pKa = 11.84WLIHH106 pKa = 5.02YY107 pKa = 9.4ARR109 pKa = 11.84LRR111 pKa = 11.84PEE113 pKa = 3.42KK114 pKa = 10.31TMEE117 pKa = 3.8EE118 pKa = 4.06RR119 pKa = 11.84LGEE122 pKa = 4.23EE123 pKa = 4.17LRR125 pKa = 11.84SAAEE129 pKa = 4.16GTGGAVKK136 pKa = 10.26KK137 pKa = 10.8RR138 pKa = 11.84SDD140 pKa = 3.42THH142 pKa = 8.38RR143 pKa = 11.84MAEE146 pKa = 3.92ANRR149 pKa = 11.84AFSHH153 pKa = 5.83YY154 pKa = 10.48RR155 pKa = 11.84FF156 pKa = 4.05

Molecular weight: 17.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1546

0

1546

338577

29

1522

219.0

24.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.264 ± 0.066

1.628 ± 0.031

4.307 ± 0.047

6.171 ± 0.076

4.975 ± 0.052

6.077 ± 0.077

2.723 ± 0.042

6.967 ± 0.055

7.032 ± 0.084

10.841 ± 0.075

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.404 ± 0.031

4.022 ± 0.059

4.175 ± 0.047

4.58 ± 0.071

4.994 ± 0.062

7.065 ± 0.061

4.916 ± 0.041

6.08 ± 0.061

1.456 ± 0.037

3.321 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski