Clostridium bornimense

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3269 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W6S2E4|W6S2E4_9CLOT TIR domain-containing protein OS=Clostridium bornimense OX=1216932 GN=CM240_2952 PE=4 SV=1
MM1 pKa = 7.29KK2 pKa = 10.5AIVNKK7 pKa = 8.24DD8 pKa = 3.22TCIGCGLCASMCPEE22 pKa = 4.5VYY24 pKa = 10.68NLDD27 pKa = 3.98DD28 pKa = 4.16EE29 pKa = 4.63EE30 pKa = 5.63GKK32 pKa = 10.73AEE34 pKa = 4.59AIEE37 pKa = 4.14EE38 pKa = 4.57EE39 pKa = 4.2IDD41 pKa = 3.35EE42 pKa = 4.38ALIDD46 pKa = 3.77EE47 pKa = 4.58AMEE50 pKa = 4.52ARR52 pKa = 11.84DD53 pKa = 3.92SCPVEE58 pKa = 5.45AIDD61 pKa = 5.13IKK63 pKa = 11.16EE64 pKa = 3.97DD65 pKa = 3.22

Molecular weight: 7.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W6S3Z3|W6S3Z3_9CLOT Uncharacterized protein OS=Clostridium bornimense OX=1216932 GN=CM240_1884 PE=4 SV=1
MM1 pKa = 7.54NILSGTYY8 pKa = 10.49GFDD11 pKa = 3.31LLSIFILIISLFFNFGKK28 pKa = 10.13IPRR31 pKa = 11.84LISLILWGIVVFRR44 pKa = 11.84AFSKK48 pKa = 9.14NTYY51 pKa = 9.51KK52 pKa = 10.77RR53 pKa = 11.84SQEE56 pKa = 3.74LSKK59 pKa = 10.39FTTFINNILRR69 pKa = 11.84KK70 pKa = 9.51FNRR73 pKa = 11.84QLPYY77 pKa = 7.8MTNVSLEE84 pKa = 4.69LIPPMFTRR92 pKa = 11.84LKK94 pKa = 8.99YY95 pKa = 9.32TINQKK100 pKa = 9.22IQYY103 pKa = 9.64KK104 pKa = 9.98IVACPNCGQKK114 pKa = 10.38LRR116 pKa = 11.84LPRR119 pKa = 11.84RR120 pKa = 11.84KK121 pKa = 9.85GKK123 pKa = 10.3IIATCKK129 pKa = 8.69RR130 pKa = 11.84CHH132 pKa = 6.13NEE134 pKa = 3.17FRR136 pKa = 11.84LRR138 pKa = 11.84TT139 pKa = 3.71

Molecular weight: 16.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3269

0

3269

1032520

29

2846

315.9

35.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.286 ± 0.052

1.148 ± 0.015

5.923 ± 0.033

7.684 ± 0.053

4.235 ± 0.03

6.405 ± 0.046

1.266 ± 0.016

10.135 ± 0.058

8.955 ± 0.048

8.677 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.543 ± 0.02

6.433 ± 0.047

2.659 ± 0.036

1.995 ± 0.021

3.346 ± 0.028

6.554 ± 0.038

5.097 ± 0.041

6.592 ± 0.039

0.702 ± 0.015

4.366 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski