Amino acid dipepetide frequency for bacterium M21

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.932AlaAla: 7.932 ± 0.206
1.185AlaCys: 1.185 ± 0.067
5.288AlaAsp: 5.288 ± 0.112
5.829AlaGlu: 5.829 ± 0.171
3.283AlaPhe: 3.283 ± 0.098
6.337AlaGly: 6.337 ± 0.154
1.735AlaHis: 1.735 ± 0.08
5.291AlaIle: 5.291 ± 0.121
4.932AlaLys: 4.932 ± 0.162
7.953AlaLeu: 7.953 ± 0.165
2.305AlaMet: 2.305 ± 0.079
3.134AlaAsn: 3.134 ± 0.115
3.164AlaPro: 3.164 ± 0.15
3.027AlaGln: 3.027 ± 0.106
4.094AlaArg: 4.094 ± 0.13
4.578AlaSer: 4.578 ± 0.154
4.837AlaThr: 4.837 ± 0.172
5.853AlaVal: 5.853 ± 0.14
1.046AlaTrp: 1.046 ± 0.06
2.32AlaTyr: 2.32 ± 0.09
0.0AlaXaa: 0.0 ± 0.0
Cys
1.072CysAla: 1.072 ± 0.057
0.294CysCys: 0.294 ± 0.034
0.749CysAsp: 0.749 ± 0.053
0.802CysGlu: 0.802 ± 0.052
0.547CysPhe: 0.547 ± 0.046
1.209CysGly: 1.209 ± 0.077
0.392CysHis: 0.392 ± 0.032
0.701CysIle: 0.701 ± 0.045
0.692CysLys: 0.692 ± 0.048
1.295CysLeu: 1.295 ± 0.066
0.374CysMet: 0.374 ± 0.03
0.422CysAsn: 0.422 ± 0.031
0.716CysPro: 0.716 ± 0.05
0.508CysGln: 0.508 ± 0.038
0.832CysArg: 0.832 ± 0.048
0.974CysSer: 0.974 ± 0.06
0.66CysThr: 0.66 ± 0.039
0.79CysVal: 0.79 ± 0.045
0.175CysTrp: 0.175 ± 0.023
0.443CysTyr: 0.443 ± 0.038
0.0CysXaa: 0.0 ± 0.0
Asp
4.914AspAla: 4.914 ± 0.13
0.811AspCys: 0.811 ± 0.052
3.755AspAsp: 3.755 ± 0.158
4.293AspGlu: 4.293 ± 0.128
2.626AspPhe: 2.626 ± 0.094
4.762AspGly: 4.762 ± 0.163
1.301AspHis: 1.301 ± 0.075
3.624AspIle: 3.624 ± 0.118
2.956AspLys: 2.956 ± 0.11
5.897AspLeu: 5.897 ± 0.134
1.518AspMet: 1.518 ± 0.072
2.109AspAsn: 2.109 ± 0.09
2.439AspPro: 2.439 ± 0.088
2.023AspGln: 2.023 ± 0.086
3.036AspArg: 3.036 ± 0.083
3.155AspSer: 3.155 ± 0.108
2.882AspThr: 2.882 ± 0.103
4.005AspVal: 4.005 ± 0.133
0.867AspTrp: 0.867 ± 0.052
2.166AspTyr: 2.166 ± 0.094
0.0AspXaa: 0.0 ± 0.0
Glu
5.603GluAla: 5.603 ± 0.172
0.701GluCys: 0.701 ± 0.053
3.583GluAsp: 3.583 ± 0.101
4.955GluGlu: 4.955 ± 0.189
2.599GluPhe: 2.599 ± 0.098
3.99GluGly: 3.99 ± 0.11
1.45GluHis: 1.45 ± 0.069
4.266GluIle: 4.266 ± 0.125
4.323GluLys: 4.323 ± 0.158
7.481GluLeu: 7.481 ± 0.193
1.762GluMet: 1.762 ± 0.076
2.481GluAsn: 2.481 ± 0.084
2.234GluPro: 2.234 ± 0.096
2.974GluGln: 2.974 ± 0.108
3.85GluArg: 3.85 ± 0.113
3.577GluSer: 3.577 ± 0.102
3.794GluThr: 3.794 ± 0.118
4.566GluVal: 4.566 ± 0.128
0.799GluTrp: 0.799 ± 0.048
1.958GluTyr: 1.958 ± 0.079
0.0GluXaa: 0.0 ± 0.0
Phe
3.197PheAla: 3.197 ± 0.11
0.674PheCys: 0.674 ± 0.049
2.846PheAsp: 2.846 ± 0.094
2.498PheGlu: 2.498 ± 0.094
1.702PhePhe: 1.702 ± 0.093
2.796PheGly: 2.796 ± 0.098
0.865PheHis: 0.865 ± 0.049
2.192PheIle: 2.192 ± 0.089
1.726PheLys: 1.726 ± 0.074
4.138PheLeu: 4.138 ± 0.122
0.98PheMet: 0.98 ± 0.059
1.393PheAsn: 1.393 ± 0.061
1.735PhePro: 1.735 ± 0.076
1.173PheGln: 1.173 ± 0.068
2.139PheArg: 2.139 ± 0.088
3.004PheSer: 3.004 ± 0.099
2.314PheThr: 2.314 ± 0.087
2.576PheVal: 2.576 ± 0.093
0.523PheTrp: 0.523 ± 0.037
1.182PheTyr: 1.182 ± 0.061
0.0PheXaa: 0.0 ± 0.0
Gly
5.324GlyAla: 5.324 ± 0.154
1.176GlyCys: 1.176 ± 0.061
4.046GlyAsp: 4.046 ± 0.131
4.551GlyGlu: 4.551 ± 0.117
2.92GlyPhe: 2.92 ± 0.096
5.368GlyGly: 5.368 ± 0.193
1.604GlyHis: 1.604 ± 0.071
4.86GlyIle: 4.86 ± 0.123
5.012GlyLys: 5.012 ± 0.167
6.254GlyLeu: 6.254 ± 0.144
2.133GlyMet: 2.133 ± 0.073
2.965GlyAsn: 2.965 ± 0.131
2.136GlyPro: 2.136 ± 0.088
2.647GlyGln: 2.647 ± 0.089
3.868GlyArg: 3.868 ± 0.141
4.43GlySer: 4.43 ± 0.162
4.759GlyThr: 4.759 ± 0.204
4.554GlyVal: 4.554 ± 0.14
0.957GlyTrp: 0.957 ± 0.059
2.418GlyTyr: 2.418 ± 0.102
0.003GlyXaa: 0.003 ± 0.003
His
1.782HisAla: 1.782 ± 0.075
0.359HisCys: 0.359 ± 0.035
1.325HisAsp: 1.325 ± 0.067
1.364HisGlu: 1.364 ± 0.062
1.061HisPhe: 1.061 ± 0.048
1.693HisGly: 1.693 ± 0.065
0.556HisHis: 0.556 ± 0.044
1.352HisIle: 1.352 ± 0.059
1.007HisLys: 1.007 ± 0.053
2.204HisLeu: 2.204 ± 0.083
0.535HisMet: 0.535 ± 0.041
0.778HisAsn: 0.778 ± 0.047
1.144HisPro: 1.144 ± 0.053
0.793HisGln: 0.793 ± 0.061
1.37HisArg: 1.37 ± 0.065
1.182HisSer: 1.182 ± 0.059
1.129HisThr: 1.129 ± 0.054
1.325HisVal: 1.325 ± 0.074
0.342HisTrp: 0.342 ± 0.035
0.755HisTyr: 0.755 ± 0.051
0.0HisXaa: 0.0 ± 0.0
Ile
5.196IleAla: 5.196 ± 0.139
1.013IleCys: 1.013 ± 0.053
3.633IleAsp: 3.633 ± 0.117
4.186IleGlu: 4.186 ± 0.133
2.374IlePhe: 2.374 ± 0.093
4.569IleGly: 4.569 ± 0.136
1.411IleHis: 1.411 ± 0.065
3.375IleIle: 3.375 ± 0.111
3.012IleLys: 3.012 ± 0.104
5.588IleLeu: 5.588 ± 0.17
1.215IleMet: 1.215 ± 0.071
2.418IleAsn: 2.418 ± 0.081
2.947IlePro: 2.947 ± 0.096
2.219IleGln: 2.219 ± 0.085
3.607IleArg: 3.607 ± 0.13
4.192IleSer: 4.192 ± 0.113
3.711IleThr: 3.711 ± 0.137
3.889IleVal: 3.889 ± 0.126
0.603IleTrp: 0.603 ± 0.042
1.723IleTyr: 1.723 ± 0.064
0.0IleXaa: 0.0 ± 0.0
Lys
4.851LysAla: 4.851 ± 0.171
0.564LysCys: 0.564 ± 0.039
3.087LysAsp: 3.087 ± 0.103
4.097LysGlu: 4.097 ± 0.152
1.616LysPhe: 1.616 ± 0.081
3.924LysGly: 3.924 ± 0.123
1.283LysHis: 1.283 ± 0.063
3.045LysIle: 3.045 ± 0.114
4.192LysLys: 4.192 ± 0.174
5.615LysLeu: 5.615 ± 0.15
1.673LysMet: 1.673 ± 0.078
2.213LysAsn: 2.213 ± 0.091
2.267LysPro: 2.267 ± 0.099
2.255LysGln: 2.255 ± 0.099
3.428LysArg: 3.428 ± 0.132
3.122LysSer: 3.122 ± 0.114
3.134LysThr: 3.134 ± 0.104
3.725LysVal: 3.725 ± 0.119
0.674LysTrp: 0.674 ± 0.053
1.649LysTyr: 1.649 ± 0.082
0.0LysXaa: 0.0 ± 0.0
Leu
8.544LeuAla: 8.544 ± 0.195
1.325LeuCys: 1.325 ± 0.071
5.466LeuAsp: 5.466 ± 0.137
6.334LeuGlu: 6.334 ± 0.158
3.806LeuPhe: 3.806 ± 0.134
6.765LeuGly: 6.765 ± 0.149
2.088LeuHis: 2.088 ± 0.074
5.838LeuIle: 5.838 ± 0.161
5.466LeuLys: 5.466 ± 0.136
9.628LeuLeu: 9.628 ± 0.216
2.323LeuMet: 2.323 ± 0.082
3.74LeuAsn: 3.74 ± 0.108
4.902LeuPro: 4.902 ± 0.133
3.731LeuGln: 3.731 ± 0.119
5.897LeuArg: 5.897 ± 0.153
6.506LeuSer: 6.506 ± 0.171
5.945LeuThr: 5.945 ± 0.173
6.156LeuVal: 6.156 ± 0.138
1.04LeuTrp: 1.04 ± 0.069
2.371LeuTyr: 2.371 ± 0.101
0.0LeuXaa: 0.0 ± 0.0
Met
2.534MetAla: 2.534 ± 0.086
0.211MetCys: 0.211 ± 0.024
1.575MetAsp: 1.575 ± 0.07
1.563MetGlu: 1.563 ± 0.071
0.9MetPhe: 0.9 ± 0.05
1.714MetGly: 1.714 ± 0.078
0.505MetHis: 0.505 ± 0.039
1.679MetIle: 1.679 ± 0.078
1.607MetLys: 1.607 ± 0.069
2.501MetLeu: 2.501 ± 0.081
0.618MetMet: 0.618 ± 0.047
1.135MetAsn: 1.135 ± 0.056
1.337MetPro: 1.337 ± 0.059
0.882MetGln: 0.882 ± 0.049
1.337MetArg: 1.337 ± 0.057
1.56MetSer: 1.56 ± 0.067
1.628MetThr: 1.628 ± 0.067
1.771MetVal: 1.771 ± 0.08
0.19MetTrp: 0.19 ± 0.022
0.526MetTyr: 0.526 ± 0.04
0.0MetXaa: 0.0 ± 0.0
Asn
2.941AsnAla: 2.941 ± 0.092
0.567AsnCys: 0.567 ± 0.037
2.198AsnAsp: 2.198 ± 0.094
2.276AsnGlu: 2.276 ± 0.078
1.301AsnPhe: 1.301 ± 0.066
3.235AsnGly: 3.235 ± 0.125
0.918AsnHis: 0.918 ± 0.052
2.228AsnIle: 2.228 ± 0.099
1.726AsnLys: 1.726 ± 0.076
3.648AsnLeu: 3.648 ± 0.1
0.847AsnMet: 0.847 ± 0.05
1.477AsnAsn: 1.477 ± 0.091
2.059AsnPro: 2.059 ± 0.094
1.384AsnGln: 1.384 ± 0.074
2.323AsnArg: 2.323 ± 0.09
2.228AsnSer: 2.228 ± 0.089
2.094AsnThr: 2.094 ± 0.105
2.599AsnVal: 2.599 ± 0.103
0.624AsnTrp: 0.624 ± 0.048
1.23AsnTyr: 1.23 ± 0.059
0.0AsnXaa: 0.0 ± 0.0
Pro
3.642ProAla: 3.642 ± 0.118
0.529ProCys: 0.529 ± 0.043
2.917ProAsp: 2.917 ± 0.095
3.687ProGlu: 3.687 ± 0.125
1.815ProPhe: 1.815 ± 0.081
3.105ProGly: 3.105 ± 0.1
0.876ProHis: 0.876 ± 0.056
2.409ProIle: 2.409 ± 0.088
2.237ProLys: 2.237 ± 0.095
3.651ProLeu: 3.651 ± 0.12
1.075ProMet: 1.075 ± 0.052
1.732ProAsn: 1.732 ± 0.066
1.586ProPro: 1.586 ± 0.071
1.506ProGln: 1.506 ± 0.075
1.839ProArg: 1.839 ± 0.073
2.32ProSer: 2.32 ± 0.091
2.478ProThr: 2.478 ± 0.086
3.342ProVal: 3.342 ± 0.112
0.597ProTrp: 0.597 ± 0.042
1.239ProTyr: 1.239 ± 0.064
0.0ProXaa: 0.0 ± 0.0
Gln
3.411GlnAla: 3.411 ± 0.11
0.38GlnCys: 0.38 ± 0.037
1.934GlnAsp: 1.934 ± 0.075
2.493GlnGlu: 2.493 ± 0.094
1.39GlnPhe: 1.39 ± 0.056
2.219GlnGly: 2.219 ± 0.091
0.817GlnHis: 0.817 ± 0.048
2.276GlnIle: 2.276 ± 0.079
2.377GlnLys: 2.377 ± 0.093
4.094GlnLeu: 4.094 ± 0.126
0.977GlnMet: 0.977 ± 0.059
1.405GlnAsn: 1.405 ± 0.066
1.477GlnPro: 1.477 ± 0.072
1.756GlnGln: 1.756 ± 0.101
2.267GlnArg: 2.267 ± 0.084
2.023GlnSer: 2.023 ± 0.08
2.059GlnThr: 2.059 ± 0.091
2.659GlnVal: 2.659 ± 0.093
0.523GlnTrp: 0.523 ± 0.044
0.986GlnTyr: 0.986 ± 0.06
0.0GlnXaa: 0.0 ± 0.0
Arg
3.96ArgAla: 3.96 ± 0.125
0.728ArgCys: 0.728 ± 0.043
2.882ArgAsp: 2.882 ± 0.104
3.731ArgGlu: 3.731 ± 0.131
2.326ArgPhe: 2.326 ± 0.089
3.134ArgGly: 3.134 ± 0.107
1.405ArgHis: 1.405 ± 0.063
3.749ArgIle: 3.749 ± 0.112
3.838ArgLys: 3.838 ± 0.117
5.915ArgLeu: 5.915 ± 0.159
1.652ArgMet: 1.652 ± 0.081
2.201ArgAsn: 2.201 ± 0.095
2.121ArgPro: 2.121 ± 0.106
2.389ArgGln: 2.389 ± 0.101
3.562ArgArg: 3.562 ± 0.125
3.161ArgSer: 3.161 ± 0.104
2.778ArgThr: 2.778 ± 0.092
3.476ArgVal: 3.476 ± 0.095
0.823ArgTrp: 0.823 ± 0.055
1.872ArgTyr: 1.872 ± 0.075
0.0ArgXaa: 0.0 ± 0.0
Ser
4.81SerAla: 4.81 ± 0.164
0.79SerCys: 0.79 ± 0.055
3.514SerAsp: 3.514 ± 0.13
3.734SerGlu: 3.734 ± 0.112
2.507SerPhe: 2.507 ± 0.091
5.172SerGly: 5.172 ± 0.19
1.331SerHis: 1.331 ± 0.075
3.731SerIle: 3.731 ± 0.107
3.087SerLys: 3.087 ± 0.1
5.746SerLeu: 5.746 ± 0.137
1.655SerMet: 1.655 ± 0.07
1.916SerAsn: 1.916 ± 0.082
2.644SerPro: 2.644 ± 0.095
2.172SerGln: 2.172 ± 0.08
3.206SerArg: 3.206 ± 0.105
4.097SerSer: 4.097 ± 0.184
3.411SerThr: 3.411 ± 0.106
4.177SerVal: 4.177 ± 0.129
0.948SerTrp: 0.948 ± 0.053
1.791SerTyr: 1.791 ± 0.073
0.0SerXaa: 0.0 ± 0.0
Thr
5.232ThrAla: 5.232 ± 0.235
0.844ThrCys: 0.844 ± 0.05
3.514ThrAsp: 3.514 ± 0.149
3.351ThrGlu: 3.351 ± 0.103
2.436ThrPhe: 2.436 ± 0.106
4.679ThrGly: 4.679 ± 0.145
1.159ThrHis: 1.159 ± 0.06
3.847ThrIle: 3.847 ± 0.167
2.79ThrLys: 2.79 ± 0.093
5.184ThrLeu: 5.184 ± 0.154
1.426ThrMet: 1.426 ± 0.07
2.195ThrAsn: 2.195 ± 0.089
3.039ThrPro: 3.039 ± 0.113
1.866ThrGln: 1.866 ± 0.075
2.727ThrArg: 2.727 ± 0.085
3.33ThrSer: 3.33 ± 0.101
3.482ThrThr: 3.482 ± 0.133
4.337ThrVal: 4.337 ± 0.204
0.657ThrTrp: 0.657 ± 0.042
1.788ThrTyr: 1.788 ± 0.087
0.0ThrXaa: 0.0 ± 0.0
Val
6.013ValAla: 6.013 ± 0.177
0.879ValCys: 0.879 ± 0.057
4.376ValAsp: 4.376 ± 0.134
4.649ValGlu: 4.649 ± 0.136
2.626ValPhe: 2.626 ± 0.102
4.245ValGly: 4.245 ± 0.132
1.245ValHis: 1.245 ± 0.064
4.239ValIle: 4.239 ± 0.12
3.262ValLys: 3.262 ± 0.096
6.586ValLeu: 6.586 ± 0.161
1.705ValMet: 1.705 ± 0.074
2.602ValAsn: 2.602 ± 0.09
2.852ValPro: 2.852 ± 0.093
2.305ValGln: 2.305 ± 0.08
3.699ValArg: 3.699 ± 0.109
4.296ValSer: 4.296 ± 0.132
4.382ValThr: 4.382 ± 0.211
4.807ValVal: 4.807 ± 0.153
0.671ValTrp: 0.671 ± 0.048
1.744ValTyr: 1.744 ± 0.077
0.0ValXaa: 0.0 ± 0.0
Trp
0.989TrpAla: 0.989 ± 0.059
0.154TrpCys: 0.154 ± 0.021
0.633TrpAsp: 0.633 ± 0.052
0.772TrpGlu: 0.772 ± 0.045
0.493TrpPhe: 0.493 ± 0.042
0.936TrpGly: 0.936 ± 0.064
0.354TrpHis: 0.354 ± 0.039
0.674TrpIle: 0.674 ± 0.045
0.838TrpLys: 0.838 ± 0.054
1.269TrpLeu: 1.269 ± 0.077
0.345TrpMet: 0.345 ± 0.031
0.511TrpAsn: 0.511 ± 0.042
0.481TrpPro: 0.481 ± 0.039
0.677TrpGln: 0.677 ± 0.046
0.737TrpArg: 0.737 ± 0.046
0.737TrpSer: 0.737 ± 0.051
0.746TrpThr: 0.746 ± 0.048
0.79TrpVal: 0.79 ± 0.043
0.19TrpTrp: 0.19 ± 0.027
0.348TrpTyr: 0.348 ± 0.033
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.175TyrAla: 2.175 ± 0.076
0.46TyrCys: 0.46 ± 0.032
1.815TyrAsp: 1.815 ± 0.079
1.78TyrGlu: 1.78 ± 0.07
1.325TyrPhe: 1.325 ± 0.062
2.133TyrGly: 2.133 ± 0.083
0.761TyrHis: 0.761 ± 0.053
1.503TyrIle: 1.503 ± 0.068
1.337TyrLys: 1.337 ± 0.07
3.223TyrLeu: 3.223 ± 0.102
0.651TyrMet: 0.651 ± 0.042
1.075TyrAsn: 1.075 ± 0.054
1.304TyrPro: 1.304 ± 0.06
1.233TyrGln: 1.233 ± 0.068
1.886TyrArg: 1.886 ± 0.075
1.967TyrSer: 1.967 ± 0.081
1.664TyrThr: 1.664 ± 0.114
1.791TyrVal: 1.791 ± 0.083
0.425TyrTrp: 0.425 ± 0.038
1.102TyrTyr: 1.102 ± 0.058
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.003XaaAsp: 0.003 ± 0.003
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1049 proteins (336607 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski